BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.5451.p (405 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777707.1| FAD dependent dehydrogenase [Herbaspirillum ... 705 0.0 ref|ZP_06064208.1| FAD dependent oxidoreductase [Acinetobacter j... 581 e-164 ref|YP_946758.1| hydroxyglutarate oxidase [Arthrobacter aurescen... 447 e-123 ref|YP_704570.1| hydroxyglutarate oxidase [Rhodococcus jostii RH... 412 e-113 ref|YP_951060.1| hydroxyglutarate oxidase [Mycobacterium vanbaal... 390 e-106 >ref|YP_003777707.1| FAD dependent dehydrogenase [Herbaspirillum seropedicae SmR1] gb|ADJ65799.1| FAD dependent dehydrogenase protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 705 bits (1820), Expect = 0.0, Method: Composition-based stats. Identities = 352/399 (88%), Positives = 372/399 (93%) Query: 1 MRPLHYAVIGGGINGLAVARQLLLDQPDVAVTVFEKEDAVARHQSSHNSGVVHAGLYYEP 60 M+PL YAVIGGGINGLAVARQLLLD PDV VTVFEKE+AVA+HQSSHNSGVVHAGLYYEP Sbjct: 1 MKPLRYAVIGGGINGLAVARQLLLDDPDVRVTVFEKEEAVAQHQSSHNSGVVHAGLYYEP 60 Query: 61 GGLKAILCRRGVQLVQSYCEQNALPYDECGKVVVALDADELPRLEAIHRKALANGVPDVE 120 GGLKA LCRRGV+LV+ YCEQNALPYDECGKVVVAL +ELPRLEAI+RKALANGVPDVE Sbjct: 61 GGLKATLCRRGVELVKRYCEQNALPYDECGKVVVALGEEELPRLEAIYRKALANGVPDVE 120 Query: 121 MIDGARLREIEPHCVGLRALYSPRTAIVSYGQIAQHMAQEIVQRGGVIRLNAAVQRVCEQ 180 MID ARLREIEP+CVGLRALYSPRTAIVSYGQIAQ MAQEI +RGG IRLNA+V+RV E+ Sbjct: 121 MIDAARLREIEPNCVGLRALYSPRTAIVSYGQIAQRMAQEIEERGGTIRLNASVRRVIER 180 Query: 181 GQHVQIELAGGEVHGDAFDFAIACCGLQSDRLAQQSGDVATPRIVPFFGQYYVIDEAFKS 240 G V IEL GE+H +AFD AIAC GLQSDRLAQQSGD ATPRIVPFFGQYYVIDEA+KS Sbjct: 181 GDQVHIELQSGEMHPEAFDSAIACSGLQSDRLAQQSGDAATPRIVPFFGQYYVIDEAYKS 240 Query: 241 HVRGLIYPVPDPRFPFLGVHFTKRIDGQMTIGPNAFISLGRENYQGDRFSLRDVADYLGY 300 HV+GLIYPVPDPRFPFLGVHFTKRIDGQMTIGPNAFISLGRENY GDRFSLRD+ADYL Y Sbjct: 241 HVKGLIYPVPDPRFPFLGVHFTKRIDGQMTIGPNAFISLGRENYHGDRFSLRDIADYLSY 300 Query: 301 PGFWKFASRNMAATLRELKTVVSERHFVREAARYVPALSEVSVTPAVRGIRAQAMEPDGR 360 PGFWKFASRN+ ATLRELKTVVSER FVREAARYVPAL+EV VTPAVRGIRAQAME +GR Sbjct: 301 PGFWKFASRNVPATLRELKTVVSERIFVREAARYVPALAEVGVTPAVRGIRAQAMESNGR 360 Query: 361 LVDDFVIRQSSRVTHIRNAPSPGATSSLAIGEHIVRNLI 399 LVDDFVIRQSSRVTHIRNAPSPGATSSLAIGEHIVRNLI Sbjct: 361 LVDDFVIRQSSRVTHIRNAPSPGATSSLAIGEHIVRNLI 399 >ref|ZP_06064208.1| FAD dependent oxidoreductase [Acinetobacter johnsonii SH046] gb|EEY95289.1| FAD dependent oxidoreductase [Acinetobacter johnsonii SH046] Length = 402 Score = 581 bits (1498), Expect = e-164, Method: Composition-based stats. Identities = 271/398 (68%), Positives = 322/398 (80%) Query: 5 HYAVIGGGINGLAVARQLLLDQPDVAVTVFEKEDAVARHQSSHNSGVVHAGLYYEPGGLK 64 HYA+IGGGINGLAVARQLLLD PD VTVFEKE VA HQSSHNSGVVHAGLYYEPGGLK Sbjct: 5 HYAIIGGGINGLAVARQLLLDYPDARVTVFEKETDVAMHQSSHNSGVVHAGLYYEPGGLK 64 Query: 65 AILCRRGVQLVQSYCEQNALPYDECGKVVVALDADELPRLEAIHRKALANGVPDVEMIDG 124 A LCRRG LVQ YC N +PYDECGKVVVAL DE RL+AI++K +AN VPDV ++ Sbjct: 65 ARLCRRGATLVQQYCTDNQIPYDECGKVVVALTKDEEGRLDAIYKKTMANQVPDVRILQA 124 Query: 125 ARLREIEPHCVGLRALYSPRTAIVSYGQIAQHMAQEIVQRGGVIRLNAAVQRVCEQGQHV 184 ++++EP+C+G +ALYSPRTAIVSYG IA+ +A+EI Q+ G + L V + EQ V Sbjct: 125 GEIQDVEPNCIGTKALYSPRTAIVSYGAIAKKIAEEIKQKNGTLVLGRKVINLTEQNNKV 184 Query: 185 QIELAGGEVHGDAFDFAIACCGLQSDRLAQQSGDVATPRIVPFFGQYYVIDEAFKSHVRG 244 + EV+ FD+ + C GLQSDRLA SGD+ATP+IVPFFGQYYVIDE FK+HV+G Sbjct: 185 SVHFDDHEVYPTQFDYVVTCAGLQSDRLASNSGDIATPKIVPFFGQYYVIDEQFKNHVKG 244 Query: 245 LIYPVPDPRFPFLGVHFTKRIDGQMTIGPNAFISLGRENYQGDRFSLRDVADYLGYPGFW 304 LIYPVPDP++PFLGVHFTKRIDGQMTIGPNAFIS GRENY G++FSL D+ D+L Y GFW Sbjct: 245 LIYPVPDPKYPFLGVHFTKRIDGQMTIGPNAFISFGRENYTGNKFSLTDIYDFLTYKGFW 304 Query: 305 KFASRNMAATLRELKTVVSERHFVREAARYVPALSEVSVTPAVRGIRAQAMEPDGRLVDD 364 KF+S+NM A +REL+TV+S+ +FV EAA+YVP+L++VSV PA RGIRAQAME DG LVDD Sbjct: 305 KFSSKNMPAAVRELRTVLSQTNFVAEAAKYVPSLADVSVEPATRGIRAQAMEADGSLVDD 364 Query: 365 FVIRQSSRVTHIRNAPSPGATSSLAIGEHIVRNLIHPH 402 FVIR+ +THIRNAPSPGATSSLAI E+IVR ++ H Sbjct: 365 FVIRKQGNITHIRNAPSPGATSSLAIAEYIVREVMSHH 402 >ref|YP_946758.1| hydroxyglutarate oxidase [Arthrobacter aurescens TC1] gb|ABM06807.1| putative FAD dependent oxidoreductase [Arthrobacter aurescens TC1] Length = 405 Score = 447 bits (1151), Expect = e-123, Method: Composition-based stats. Identities = 218/389 (56%), Positives = 284/389 (73%), Gaps = 3/389 (0%) Query: 7 AVIGGGINGLAVARQLLLDQPDVAVTVFEKEDAVARHQSSHNSGVVHAGLYYEPGGLKAI 66 AV+GGGI G+AVAR+L DV VTV+EKED +A HQ+ HNSGVVHAGLYYEPGGLKA Sbjct: 14 AVVGGGIIGIAVARELTNRLHDVQVTVYEKEDRLAAHQTGHNSGVVHAGLYYEPGGLKAK 73 Query: 67 LCRRGVQLVQSYCEQNALPYDECGKVVVALDADELPRLEAIHRKALANGVPDVEMIDGAR 126 LCRRGV+L+Q +C LPY+ CGK+V+A +E RL+ I +A ANGVP V M+ G + Sbjct: 74 LCRRGVELLQEFCTAKNLPYEACGKLVIAQTPEESQRLDNIFARATANGVPGVRMLRGEQ 133 Query: 127 LREIEPHCVGLRALYSPRTAIVSYGQIAQHMAQEIVQRGGVIRLNAAVQRVCEQGQHVQI 186 +RE+EP+ VGL AL+SP TAIV Y I +A ++ GG IRL V + +QG V + Sbjct: 134 IREVEPNAVGLSALHSPETAIVDYTAITNALADDVRGSGGAIRLGQEVTSLAQQGSGVVV 193 Query: 187 ELAGGEVHGDAFDFAIACCGLQSDRLAQQSGDVATPRIVPFFGQYYVIDEAFKSHVRGLI 246 G G+ +D + C GLQSDR+A+ +G+ ATPRIVPFFGQY+++ + + HVRGLI Sbjct: 194 TTKDG---GEHYDLVVVCAGLQSDRVAEATGEPATPRIVPFFGQYFLLGKEARDHVRGLI 250 Query: 247 YPVPDPRFPFLGVHFTKRIDGQMTIGPNAFISLGRENYQGDRFSLRDVADYLGYPGFWKF 306 YPVPDP+ PFLGVH TKRIDG+M +GPNAFIS GRE+Y ++ ++RD+ +Y +PGFW F Sbjct: 251 YPVPDPKHPFLGVHLTKRIDGEMMLGPNAFISFGRESYAWNQVNVRDILNYTLFPGFWNF 310 Query: 307 ASRNMAATLRELKTVVSERHFVREAARYVPALSEVSVTPAVRGIRAQAMEPDGRLVDDFV 366 A +N+ + +RE +TVVS++ F+REA R+VP+L +V P RG+RAQAM DG LVDDFV Sbjct: 311 ARQNVPSAVREFQTVVSKKRFIREAVRFVPSLEGATVLPGTRGVRAQAMNGDGSLVDDFV 370 Query: 367 IRQSSRVTHIRNAPSPGATSSLAIGEHIV 395 I + +RNAPSPGATSS+AI E+IV Sbjct: 371 IARRRDAVLVRNAPSPGATSSMAIAEYIV 399 >ref|YP_704570.1| hydroxyglutarate oxidase [Rhodococcus jostii RHA1] gb|ABG96412.1| conserved hypothetical protein [Rhodococcus jostii RHA1] Length = 401 Score = 412 bits (1060), Expect = e-113, Method: Composition-based stats. Identities = 214/395 (54%), Positives = 270/395 (68%), Gaps = 3/395 (0%) Query: 1 MRPLHYAVIGGGINGLAVARQLLLDQPDVAVTVFEKEDAVARHQSSHNSGVVHAGLYYEP 60 M + AVIGGGI GLAVAR+LL V VT+FEKE VA HQ+ HNSGVVHAGLYY P Sbjct: 1 MAKIRAAVIGGGIIGLAVARELLHRMDAVEVTLFEKETRVAAHQTGHNSGVVHAGLYYLP 60 Query: 61 GGLKAILCRRGVQLVQSYCEQNALPYDECGKVVVALDADELPRLEAIHRKALANGVPDVE 120 G LKA LCRRGV L+Q Y E + Y+ECGKVVVA D+ E+ R++AI +A+ANGVP + Sbjct: 61 GSLKARLCRRGVTLLQQYAENKGVAYEECGKVVVAHDSSEVARMDAIFDRAVANGVPGIR 120 Query: 121 MIDGARLREIEPHCVGLRALYSPRTAIVSYGQIAQHMAQEIVQRGGVIRLNAAVQRVCEQ 180 + G + EIEPH G+ A++SP TAI+ Y +A+ +A +I GG + L V + + Sbjct: 121 KVAGEEIPEIEPHARGVAAVHSPHTAIIDYVAVAEALAADITAAGGRVLLGREVVGLDSR 180 Query: 181 GQHVQIELAGGEVHGDAFDFAIACCGLQSDRLAQQSGDVATPRIVPFFGQYYVIDEAFKS 240 + G + FD + C GLQSDR+A SG+ +PR+VPFFG Y++++ S Sbjct: 181 TAETVVTTGQGS---EVFDLVVTCAGLQSDRVALMSGEPRSPRVVPFFGDYFLLEPERSS 237 Query: 241 HVRGLIYPVPDPRFPFLGVHFTKRIDGQMTIGPNAFISLGRENYQGDRFSLRDVADYLGY 300 V+GLIYPVPDPR+PFLGVH TKRIDG++ +GPNAF+S GRE Y +S DV +G+ Sbjct: 238 LVKGLIYPVPDPRYPFLGVHLTKRIDGRIMLGPNAFLSFGREAYDHRGWSASDVMSAVGF 297 Query: 301 PGFWKFASRNMAATLRELKTVVSERHFVREAARYVPALSEVSVTPAVRGIRAQAMEPDGR 360 PGFW+FA N AA +RE +TV+S FV+EA +YVP + VT RGIRAQAM DG Sbjct: 298 PGFWRFAVHNTAAAVREARTVLSTGQFVKEAQKYVPDVRRSDVTRGPRGIRAQAMNADGS 357 Query: 361 LVDDFVIRQSSRVTHIRNAPSPGATSSLAIGEHIV 395 L DDFVI S RV H+RNAPSPGATSSLAI EH+V Sbjct: 358 LEDDFVITGSERVIHVRNAPSPGATSSLAIAEHVV 392 >ref|YP_951060.1| hydroxyglutarate oxidase [Mycobacterium vanbaalenii PYR-1] gb|ABM11054.1| FAD dependent oxidoreductase [Mycobacterium vanbaalenii PYR-1] Length = 402 Score = 390 bits (1002), Expect = e-106, Method: Composition-based stats. Identities = 207/381 (54%), Positives = 260/381 (68%), Gaps = 3/381 (0%) Query: 19 ARQLLLDQPDVAVTVFEKEDAVARHQSSHNSGVVHAGLYYEPGGLKAILCRRGVQLVQSY 78 AR+LL VTVFEKE VARHQ+ HNSGVVHAGLYY PG LKA LCRRGV L++ Y Sbjct: 19 ARELLHSIDGARVTVFEKESGVARHQTGHNSGVVHAGLYYAPGSLKARLCRRGVHLLREY 78 Query: 79 CEQNALPYDECGKVVVALDADELPRLEAIHRKALANGVPDVEMIDGARLREIEPHCVGLR 138 Q + Y ECGK+VVA A + RL IH +ALANGVP V ++D + +IEPH G+ Sbjct: 79 LAQKDIRYSECGKIVVAHTAGDAARLAGIHERALANGVPGVRLVDAGEITDIEPHARGIS 138 Query: 139 ALYSPRTAIVSYGQIAQHMAQEIVQRGGVIRLNAAVQRVCEQGQHVQIELAGGEVHGDAF 198 AL+SP TAIV Y +A+ +A +I GG + + V + G V + A AF Sbjct: 139 ALHSPSTAIVDYPAVAEALAVDIADAGGEVLCDHGVIGMRRHGTEVVVTTAATS---GAF 195 Query: 199 DFAIACCGLQSDRLAQQSGDVATPRIVPFFGQYYVIDEAFKSHVRGLIYPVPDPRFPFLG 258 D + C GLQSDR+A+ GD ++PRIVPFFG Y+++ EA V GLIYPVPDPR+PFLG Sbjct: 196 DVVVTCAGLQSDRVARMFGDSSSPRIVPFFGDYFLLHEAKSDMVNGLIYPVPDPRYPFLG 255 Query: 259 VHFTKRIDGQMTIGPNAFISLGRENYQGDRFSLRDVADYLGYPGFWKFASRNMAATLREL 318 VH TK +DG++++GPNAF++ GRE Y +S RDVA +G+PGFW+FA RN AA +RE Sbjct: 256 VHLTKHVDGRVSLGPNAFLAPGREAYTRGGWSARDVAAAVGFPGFWRFAVRNTAAAVREA 315 Query: 319 KTVVSERHFVREAARYVPALSEVSVTPAVRGIRAQAMEPDGRLVDDFVIRQSSRVTHIRN 378 +TV+S FVREA +YVP +S V RGIRAQAM DG L DDFVI + +V H+RN Sbjct: 316 RTVMSRAAFVREAQKYVPDVSVDDVVRGPRGIRAQAMNADGTLEDDFVITGTDKVIHLRN 375 Query: 379 APSPGATSSLAIGEHIVRNLI 399 APSPGATSSLAI E+IV ++ Sbjct: 376 APSPGATSSLAIAEYIVGTVV 396