BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.5376.p (411 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777636.1| transmembrane protein [Herbaspirillum serope... 766 0.0 ref|YP_003751971.1| hypothetical protein RPSI07_1318 [Ralstonia ... 564 e-159 emb|CCA84482.1| conserved membrane hypothetical protein, DUF989 ... 557 e-156 emb|CBJ37455.1| conserved membrane protein of unknown function, ... 550 e-154 ref|YP_002981885.1| hypothetical protein Rpic12D_1932 [Ralstonia... 543 e-152 >ref|YP_003777636.1| transmembrane protein [Herbaspirillum seropedicae SmR1] gb|ADJ65728.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 766 bits (1978), Expect = 0.0, Method: Composition-based stats. Identities = 400/411 (97%), Positives = 408/411 (99%) Query: 1 MEAYLLDWLNLLLRWLHVITAIAWIGSSFYFVWLDNSLVAPTDPELKEKGVGGELWAVHG 60 MEAY+LDWLNLLLRWLHVITAIAWIGSSFYFVWLDNSLVAPTDPELKEKGVGGELWAVHG Sbjct: 1 MEAYILDWLNLLLRWLHVITAIAWIGSSFYFVWLDNSLVAPTDPELKEKGVGGELWAVHG 60 Query: 61 GGFYNPQKYLLAPKVLPEHLHWFYWESYSTWLSGFALFSLLYLFNANVFLVDRNVFDMSA 120 GGFYNPQKYLLAPKVLPEHLHWFYWESYSTWLSGFALFSLLYLFNANVFLVDRNVFDMSA Sbjct: 61 GGFYNPQKYLLAPKVLPEHLHWFYWESYSTWLSGFALFSLLYLFNANVFLVDRNVFDMSA 120 Query: 121 SMAGGTAIAFLVFNWLIYDAICSTIGDRPGADRLVNILVGLQVIVSVWAACHLFSGRAAF 180 SMAGG AIAFL+FNWLIYDAICSTIGDRPGADRLVN+LVGLQV+VSVWAACHLFSGRAAF Sbjct: 121 SMAGGAAIAFLIFNWLIYDAICSTIGDRPGADRLVNLLVGLQVVVSVWAACHLFSGRAAF 180 Query: 181 LISGASLATIMSANVLVWIIPGQRKMVASMRAGQAPDPIHGKRGKQRSVHNTYFTLPVLF 240 LISGASLATIMSANVLVWIIPGQRKMVASMRAGQAPDPIHGKRGKQRSVHNTYFTLPVLF Sbjct: 181 LISGASLATIMSANVLVWIIPGQRKMVASMRAGQAPDPIHGKRGKQRSVHNTYFTLPVLF 240 Query: 241 AMLSNHYSMTYSAPHNWIVLLCIMAAGALIRQFFVLRHKGVYNWWYPGVAVALLLAVILW 300 AMLSNHYSMTY+APHNW+VLL IMAAGALIRQFFVLRHKGVYNWWYPG+AV LLLAVI+W Sbjct: 241 AMLSNHYSMTYNAPHNWVVLLVIMAAGALIRQFFVLRHKGVYNWWYPGIAVGLLLAVIVW 300 Query: 301 MSPLMQARPVVVAASSSSSSSSSSSEGGAFAQVQQVIQARCIQCHAAQPTLMPVPGKGVL 360 MSPLMQARPVVVAASSSSSSSSSSSEGGAFAQVQQVIQARCIQCHAAQPTLMPVPGKGVL Sbjct: 301 MSPLMQARPVVVAASSSSSSSSSSSEGGAFAQVQQVIQARCIQCHAAQPTLMPVPGKGVL 360 Query: 361 LDSPEHISQHAQQIYQQAVVQKAMPLGNMTHITEEERAVIGKWFEAGAAVK 411 LDSPEHISQHAQQIYQQAVVQKAMPLGNMTHITEEERAVIGKWFEAGAAVK Sbjct: 361 LDSPEHISQHAQQIYQQAVVQKAMPLGNMTHITEEERAVIGKWFEAGAAVK 411 >ref|YP_003751971.1| hypothetical protein RPSI07_1318 [Ralstonia solanacearum PSI07] emb|CBJ50678.1| conserved membrane protein of unknown function, DUF989 [Ralstonia solanacearum PSI07] emb|CCA81951.1| conserved membrane hypothetical protein,DUF989 [blood disease bacterium R229] Length = 407 Score = 564 bits (1454), Expect = e-159, Method: Composition-based stats. Identities = 281/408 (68%), Positives = 336/408 (82%), Gaps = 5/408 (1%) Query: 1 MEAYLLDWLNLLLRWLHVITAIAWIGSSFYFVWLDNSLVAPTDPELKEKGVGGELWAVHG 60 ME YLLDW NLLLRWLHVITAIAWIGSSFYFVWLDNSL PT P+L EKGV GELWAVHG Sbjct: 1 MEGYLLDWANLLLRWLHVITAIAWIGSSFYFVWLDNSLTRPTAPDLLEKGVDGELWAVHG 60 Query: 61 GGFYNPQKYLLAPKVLPEHLHWFYWESYSTWLSGFALFSLLYLFNANVFLVDRNVFDMSA 120 GGFY+PQKYLLAP+ LP+HLHWFYWESY+TW+SGFAL +++YLFNANV+L+DR+VFDM+A Sbjct: 61 GGFYHPQKYLLAPRQLPDHLHWFYWESYTTWMSGFALLTVVYLFNANVYLIDRSVFDMTA 120 Query: 121 SMAGGTAIAFLVFNWLIYDAICSTIGDRPGADRLVNILVGLQVIVSVWAACHLFSGRAAF 180 + A A+AFL WL+YDAIC G +DRLV +LV + V+++ +AACHLFSGRAAF Sbjct: 121 TTAVLLALAFLAVGWLVYDAICRVFGK---SDRLVGVLVAIYVVIAAYAACHLFSGRAAF 177 Query: 181 LISGASLATIMSANVLVWIIPGQRKMVASMRAGQAPDPIHGKRGKQRSVHNTYFTLPVLF 240 L+ GA +ATIMSANV WIIPGQRK+VA+++AG+ PDPIHGKRGKQRSVHNTYFTLPVLF Sbjct: 178 LLIGAMIATIMSANVFFWIIPGQRKVVAALKAGEKPDPIHGKRGKQRSVHNTYFTLPVLF 237 Query: 241 AMLSNHYSMTYSAPHNWIVLLCIMAAGALIRQFFVLRHKGVYNWWYPGVAVALLLAVILW 300 AMLSNHYSMTYS NWIVL+ IM AG LIRQFF+L+HKGV+NWWYP VALLL +W Sbjct: 238 AMLSNHYSMTYSHTSNWIVLVLIMLAGVLIRQFFILKHKGVWNWWYPAGGVALLLGTAVW 297 Query: 301 MSPLMQARPVVVAASSSSSSSSSSSEGGAFAQVQQVIQARCIQCHAAQPTLMPVPGKGVL 360 ++P+ RP A+++S + ++S G FA+VQ V+ ARC QCH+A PTLMP P KGVL Sbjct: 298 LAPVQ--RPAAPEANAASVTVPAASGGSDFAKVQAVVTARCYQCHSAHPTLMPSPAKGVL 355 Query: 361 LDSPEHISQHAQQIYQQAVVQKAMPLGNMTHITEEERAVIGKWFEAGA 408 LD+PE ++ HAQ +YQQAV QK MPLGN+T +T+EER +I KWFE GA Sbjct: 356 LDTPEQLAAHAQLVYQQAVQQKLMPLGNVTQMTDEERTLIAKWFEGGA 403 >emb|CCA84482.1| conserved membrane hypothetical protein, DUF989 [Ralstonia syzygii R24] Length = 407 Score = 557 bits (1435), Expect = e-156, Method: Composition-based stats. Identities = 280/408 (68%), Positives = 336/408 (82%), Gaps = 5/408 (1%) Query: 1 MEAYLLDWLNLLLRWLHVITAIAWIGSSFYFVWLDNSLVAPTDPELKEKGVGGELWAVHG 60 ME YLLDW NLLLRWLHVITAIAWIGSSFYFVWLDNSL PT P+L EKGV GELWAVHG Sbjct: 1 MEGYLLDWANLLLRWLHVITAIAWIGSSFYFVWLDNSLTRPTAPDLLEKGVDGELWAVHG 60 Query: 61 GGFYNPQKYLLAPKVLPEHLHWFYWESYSTWLSGFALFSLLYLFNANVFLVDRNVFDMSA 120 GGFY+PQKYLLAP+ LPEHLHWFYWESY+TW+SGFAL +++YLFNANV+L+DR+VFDM+A Sbjct: 61 GGFYHPQKYLLAPRQLPEHLHWFYWESYTTWMSGFALLTVVYLFNANVYLIDRSVFDMTA 120 Query: 121 SMAGGTAIAFLVFNWLIYDAICSTIGDRPGADRLVNILVGLQVIVSVWAACHLFSGRAAF 180 + A A+AFL WL+YDAIC G +DRLV +LV + V+++ +AACHLFSGRAAF Sbjct: 121 TTAVLLALAFLAVGWLVYDAICRVFGK---SDRLVGVLVAIYVVIAAYAACHLFSGRAAF 177 Query: 181 LISGASLATIMSANVLVWIIPGQRKMVASMRAGQAPDPIHGKRGKQRSVHNTYFTLPVLF 240 L+ GA +ATIMSANV WIIPGQRK+VA+++AG+ PDPIHGKRGKQRSVHNTYFTLPVLF Sbjct: 178 LLIGAMIATIMSANVFFWIIPGQRKVVAALKAGEKPDPIHGKRGKQRSVHNTYFTLPVLF 237 Query: 241 AMLSNHYSMTYSAPHNWIVLLCIMAAGALIRQFFVLRHKGVYNWWYPGVAVALLLAVILW 300 AMLSNHYSMTYS NW+VL+ IM AG LIRQFF+L+HKGV+NWWYP VALLL +W Sbjct: 238 AMLSNHYSMTYSHTSNWVVLVLIMLAGVLIRQFFILKHKGVWNWWYPAGGVALLLGTAVW 297 Query: 301 MSPLMQARPVVVAASSSSSSSSSSSEGGAFAQVQQVIQARCIQCHAAQPTLMPVPGKGVL 360 ++P+ RP A++++ + ++S G FA+VQ V+ ARC QCH+A PTLMP P KGVL Sbjct: 298 LAPVQ--RPAAPEANAAAVTVPAASGGSDFAKVQAVVTARCYQCHSAHPTLMPSPAKGVL 355 Query: 361 LDSPEHISQHAQQIYQQAVVQKAMPLGNMTHITEEERAVIGKWFEAGA 408 LD+PE ++ HAQ +YQQAV QK MPLGN+T +T+EER +I KWFE GA Sbjct: 356 LDTPEQLAAHAQLVYQQAVQQKLMPLGNVTQMTDEERTLIAKWFEGGA 403 >emb|CBJ37455.1| conserved membrane protein of unknown function, DUF989 [Ralstonia solanacearum CMR15] Length = 400 Score = 550 bits (1416), Expect = e-154, Method: Composition-based stats. Identities = 278/408 (68%), Positives = 328/408 (80%), Gaps = 12/408 (2%) Query: 1 MEAYLLDWLNLLLRWLHVITAIAWIGSSFYFVWLDNSLVAPTDPELKEKGVGGELWAVHG 60 ME YLLDW NLLLRWLHVITAIAWIGSSFYFVWLDNSL PT P+L +KGV GELWAVHG Sbjct: 1 MEGYLLDWANLLLRWLHVITAIAWIGSSFYFVWLDNSLTRPTTPDLLDKGVDGELWAVHG 60 Query: 61 GGFYNPQKYLLAPKVLPEHLHWFYWESYSTWLSGFALFSLLYLFNANVFLVDRNVFDMSA 120 GGFY+PQKYLLAPK LPEHLHWFYWESYSTW+SGFAL +++YLFNANV+L+DR+VFDM+A Sbjct: 61 GGFYHPQKYLLAPKQLPEHLHWFYWESYSTWMSGFALLTVVYLFNANVYLIDRSVFDMTA 120 Query: 121 SMAGGTAIAFLVFNWLIYDAICSTIGDRPGADRLVNILVGLQVIVSVWAACHLFSGRAAF 180 + A A+AFL WL+YD IC G R DRLV LV + V+++ +AACHLF+GRAAF Sbjct: 121 TTAVLLALAFLAVGWLVYDTICRVFGKR---DRLVGALVAVYVVIAAYAACHLFAGRAAF 177 Query: 181 LISGASLATIMSANVLVWIIPGQRKMVASMRAGQAPDPIHGKRGKQRSVHNTYFTLPVLF 240 L+ GA +ATIMSANV WIIPGQRK+VA+++AG+ PDPIHGKRGKQRSVHNTYFTLPVLF Sbjct: 178 LLIGAMIATIMSANVFFWIIPGQRKVVAALKAGEQPDPIHGKRGKQRSVHNTYFTLPVLF 237 Query: 241 AMLSNHYSMTYSAPHNWIVLLCIMAAGALIRQFFVLRHKGVYNWWYPGVAVALLLAVILW 300 AMLSNHYSMTY+ P NW+VL+ IM AG LIRQFF+L+HKGV+N WYP VALLL +W Sbjct: 238 AMLSNHYSMTYAHPSNWVVLVLIMLAGVLIRQFFILKHKGVWNGWYPAGGVALLLGTAVW 297 Query: 301 MSPLMQARPVVVAASSSSSSSSSSSEGGAFAQVQQVIQARCIQCHAAQPTLMPVPGKGVL 360 ++P+ RP +++S S FA+VQ V+ ARC QCH+ PTLMP P KGVL Sbjct: 298 LAPVQ--RPAAQGGAAASGGSD-------FAKVQAVVTARCYQCHSTHPTLMPSPAKGVL 348 Query: 361 LDSPEHISQHAQQIYQQAVVQKAMPLGNMTHITEEERAVIGKWFEAGA 408 LD+PE ++ HAQ +YQQAV QK MPLGN+T +T+EER VI KWFE GA Sbjct: 349 LDTPEQLAAHAQLVYQQAVQQKLMPLGNITQMTDEERTVIAKWFEDGA 396 >ref|YP_002981885.1| hypothetical protein Rpic12D_1932 [Ralstonia pickettii 12D] gb|ACS63213.1| protein of unknown function DUF989 [Ralstonia pickettii 12D] Length = 425 Score = 543 bits (1398), Expect = e-152, Method: Composition-based stats. Identities = 277/426 (65%), Positives = 337/426 (79%), Gaps = 22/426 (5%) Query: 1 MEAYLLDWLNLLLRWLHVITAIAWIGSSFYFVWLDNSLVAPTDPELKEKGVGGELWAVHG 60 ME Y+LDW NLLLRWLHVI AIAWIGSSFYFVWLDNSL PT P+LK+KGV GELWAVHG Sbjct: 1 MEGYILDWANLLLRWLHVIVAIAWIGSSFYFVWLDNSLTKPTAPDLKDKGVDGELWAVHG 60 Query: 61 GGFYNPQKYLLAPKVLPEHLHWFYWESYSTWLSGFALFSLLYLFNANVFLVDRNVFDMSA 120 GGFYNPQKYLLAPK LP+HLHWFYWESYSTW+SGFAL +++YLFNANV+++DR+VFDM+A Sbjct: 61 GGFYNPQKYLLAPKQLPDHLHWFYWESYSTWMSGFALLTVVYLFNANVYMIDRSVFDMTA 120 Query: 121 SMAGGTAIAFLVFNWLIYDAICSTIGDRPGADRLVNILVGLQVIVSVWAACHLFSGRAAF 180 + A A+ FLV WL+YD IC G DR+V ILV + V+++ +AACHLFSGRAAF Sbjct: 121 TTAVLLALGFLVVGWLVYDTICRVFGKN---DRVVGILVAIYVVIAAFAACHLFSGRAAF 177 Query: 181 LISGASLATIMSANVLVWIIPGQRKMVASMRAGQAPDPIHGKRGKQRSVHNTYFTLPVLF 240 L+ GA +ATIMSANV WIIPGQRK+VAS++AG+ PDPIHGKRGKQRSVHNTYFTLPVLF Sbjct: 178 LLIGAMIATIMSANVFFWIIPGQRKVVASLKAGEKPDPIHGKRGKQRSVHNTYFTLPVLF 237 Query: 241 AMLSNHYSMTYSAPHNWIVLLCIMAAGALIRQFFVLRHKGVYNWWYPGVAVALLLAVILW 300 AMLSNHYSMTY+A +NW+VL+ IM AG LIRQFF+L+HKGV NW YP VA+LL V +W Sbjct: 238 AMLSNHYSMTYAAKYNWVVLVLIMLAGVLIRQFFILKHKGVVNWGYPAAGVAVLLGVAVW 297 Query: 301 MSPLMQARPVVVAASSSSSSSSSSSEGGA------------------FAQVQQVIQARCI 342 ++P+ + VA + ++++ ++++EG A FA+VQ V+ ARC Sbjct: 298 LAPVSRPA-APVAEAPAATAPAAAAEGSASTPAAAGQASAAAAGSSDFAKVQAVVTARCY 356 Query: 343 QCHAAQPTLMPVPGKGVLLDSPEHISQHAQQIYQQAVVQKAMPLGNMTHITEEERAVIGK 402 QCH+A PTLMP P KGVLLD+PE + +HAQ +YQQ V QK MPLGN+T IT++ER +I K Sbjct: 357 QCHSAHPTLMPSPAKGVLLDTPEELGKHAQLVYQQVVQQKLMPLGNVTQITDDERTIIAK 416 Query: 403 WFEAGA 408 WFE GA Sbjct: 417 WFEGGA 422