BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.5273.p (296 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777517.1| drug/metabolite transporter (DMT) superfamil... 372 e-101 ref|YP_004152564.1| hypothetical protein Varpa_0231 [Variovorax ... 184 2e-44 emb|CAZ87491.1| putative Permease of the drug/metabolite transpo... 180 2e-43 ref|YP_003642332.1| protein of unknown function DUF6 transmembra... 179 6e-43 ref|YP_002942156.1| hypothetical protein Vapar_0227 [Variovorax ... 171 1e-40 >ref|YP_003777517.1| drug/metabolite transporter (DMT) superfamily permease [Herbaspirillum seropedicae SmR1] gb|ADJ65609.1| permease of the drug/metabolite transporter (DMT) superfamily protein [Herbaspirillum seropedicae SmR1] Length = 295 Score = 372 bits (956), Expect = e-101, Method: Composition-based stats. Identities = 239/296 (80%), Positives = 263/296 (88%), Gaps = 1/296 (0%) Query: 1 MQHLPKPAVAALLLNTFIWGLSWTGTRALEGMGLHPVWATAAIFSGCALLLLTLRHRELP 60 MQ LPK AVAALL+NT IWGLSWT TRALEGMGLHPVW TAAIF C LLL+ LR+R+LP Sbjct: 1 MQVLPKSAVAALLVNTTIWGLSWTATRALEGMGLHPVWTTAAIFGLCTLLLVALRYRDLP 60 Query: 61 LLWRHPALLGVGLATGLTNASFNMAVAYGDVVRVILLFYLMPVWTVILARLVLHEAITPR 120 LWRHPALL VGLATGLTN++FN+AVAYGDVVRVILLFYLMPVWTVILARLVLHEAITPR Sbjct: 61 QLWRHPALLWVGLATGLTNSTFNVAVAYGDVVRVILLFYLMPVWTVILARLVLHEAITPR 120 Query: 121 SLARVALGLGGAFIVLYQPRLGLPLPQSLPDWMAVASGLLFAFTNVLLRRMHGLSSDGAR 180 SL RVALGL GAFIVLYQPRLGLPLPQSLPDWMAVA+GLLFA TNVLLRR+ GL SD AR Sbjct: 121 SLTRVALGLSGAFIVLYQPRLGLPLPQSLPDWMAVAAGLLFAITNVLLRRLQGL-SDSAR 179 Query: 181 AVAMLAGSGLLATLLGLLLSLLGMIAWPLQVSAAAAPVVGVWALLFLFSNLCLQYGVSRL 240 AVAMLAGSGLLA +LG++L+L G IAWPL V A A P+VGVWALLFL SNLCLQYGV+RL Sbjct: 180 AVAMLAGSGLLAGVLGVMLALAGAIAWPLHVPATAWPLVGVWALLFLVSNLCLQYGVARL 239 Query: 241 PANITAVVMLTEILVASLSSWALGAAELRVQDLVGGVLIIAAPWIVRERQVSAQAA 296 PA IT+V+ML EILVASLS+W GAAELR QDL+GG+LIIAAPW+VR+ + AQ+A Sbjct: 240 PATITSVIMLAEILVASLSAWWFGAAELRPQDLIGGILIIAAPWLVRDSRPVAQSA 295 >ref|YP_004152564.1| hypothetical protein Varpa_0231 [Variovorax paradoxus EPS] gb|ADU34453.1| protein of unknown function DUF6 transmembrane [Variovorax paradoxus EPS] Length = 296 Score = 184 bits (466), Expect = 2e-44, Method: Composition-based stats. Identities = 126/274 (45%), Positives = 171/274 (62%), Gaps = 9/274 (3%) Query: 11 ALLLNTFIWGLSWTGTRALEGMGLHPVWATAAIFSGCALLLLTLRHRELPLLWRHPALLG 70 AL+ N F+WGLSW R +E GLHP+W T I+ A ++ +R +P L Sbjct: 5 ALIFNAFVWGLSWFPFRHIESHGLHPLWTTCLIYLAIATVMGIVRRHAWKSFTAYPMLAV 64 Query: 71 VGLATGLTNASFNMAVAYGDVVRVILLFYLMPVWTVILARLVLHEAITPRSLARVALGLG 130 +GLA G TN FN AV GDVVRV+LLFYLMP+W+V+L L+L E T +LARVAL L Sbjct: 65 LGLAAGFTNVGFNWAVTQGDVVRVVLLFYLMPLWSVLLGWLLLGERPTGGALARVALALT 124 Query: 131 GAFIVLYQPRLGLPLPQSLPDWMAVASGLLFAFTNVLLRRMHGLSSDGARAVAMLAGSGL 190 G +VL P P+P SLPDW+ +A+G FA TN++LR + + +RA+AM G Sbjct: 125 GVVVVLKAPGTDWPVPSSLPDWLGLAAGFSFAVTNIVLRHLRPAPGE-SRALAMFCGCTF 183 Query: 191 LATLLGLLLSLLGMIAWPLQVSAAAAPVVGVWALL----FLFSNLCLQYGVSRLPANITA 246 +A + L+ + LG IA PLQ A P WA+L F+ N+CLQYG +RL A+ T+ Sbjct: 184 VAGIAALVGTSLGAIASPLQ----ATPGWLGWAVLLGAGFVIGNVCLQYGAARLAASATS 239 Query: 247 VVMLTEILVASLSSWALGAAELRVQDLVGGVLII 280 V+ML+E+L AS+SS ALGA+ + + L GG LI+ Sbjct: 240 VIMLSEVLFASVSSVALGASTMDPRILTGGALIV 273 >emb|CAZ87491.1| putative Permease of the drug/metabolite transporter (DMT) superfamily [Thiomonas sp. 3As] Length = 312 Score = 180 bits (457), Expect = 2e-43, Method: Composition-based stats. Identities = 123/269 (45%), Positives = 167/269 (62%), Gaps = 1/269 (0%) Query: 8 AVAALLLNTFIWGLSWTGTRALEGMGLHPVWATAAIFSGCALLLLTLRHRELPLLWRHPA 67 A+ L+LN F+WG+SW R G+GL+P+WATAA+++ L+++ R + LWRHP Sbjct: 11 AIGGLMLNAFVWGVSWWPFRHFNGLGLNPLWATAAMYAVSTLIIVAARPSAVRALWRHPP 70 Query: 68 LLGVGLATGLTNASFNMAVAYGDVVRVILLFYLMPVWTVILARLVLHEAITPRSLARVAL 127 L G+ +A+G TNA+FN V GDVVRV+LLFYLMPVW +LAR +L+E +T R+ L Sbjct: 71 LWGILIASGATNAAFNWGVTEGDVVRVVLLFYLMPVWATLLARWLLNEKLTRLVGLRLTL 130 Query: 128 GLGGAFIVLYQPRLGLPLPQSLPDWMAVASGLLFAFTNVLLRRMHGLSSDGARAVAMLAG 187 L GA +VL+ GLPLPQ DW+ V G+ FA NVLLR+ + + AR +AM AG Sbjct: 131 ALAGAMVVLWPQGGGLPLPQHPADWLGVIGGMFFALNNVLLRK-YAHTPPEARGLAMFAG 189 Query: 188 SGLLATLLGLLLSLLGMIAWPLQVSAAAAPVVGVWALLFLFSNLCLQYGVSRLPANITAV 247 ++A + LL+ G +A + V + AL FL +NL LQYG RLP+ +TAV Sbjct: 190 GVIVAGGVAALLTASGAVAPMPAPAPGWLMGVVLMALAFLAANLGLQYGAERLPSTLTAV 249 Query: 248 VMLTEILVASLSSWALGAAELRVQDLVGG 276 VM EI+ AS S+ LG L Q L GG Sbjct: 250 VMTVEIVFASGSAVLLGVERLSAQALWGG 278 >ref|YP_003642332.1| protein of unknown function DUF6 transmembrane [Thiomonas intermedia K12] gb|ADG30002.1| protein of unknown function DUF6 transmembrane [Thiomonas intermedia K12] Length = 312 Score = 179 bits (453), Expect = 6e-43, Method: Composition-based stats. Identities = 124/269 (46%), Positives = 166/269 (61%), Gaps = 1/269 (0%) Query: 8 AVAALLLNTFIWGLSWTGTRALEGMGLHPVWATAAIFSGCALLLLTLRHRELPLLWRHPA 67 A+ L+LN F+WG+SW R G+GL+P+WATAA+++ L+++ R L LWRHP Sbjct: 11 AIGGLMLNAFVWGVSWWPFRHFNGLGLNPLWATAAMYAVSTLIIVAARPSALRALWRHPP 70 Query: 68 LLGVGLATGLTNASFNMAVAYGDVVRVILLFYLMPVWTVILARLVLHEAITPRSLARVAL 127 L G+ +A+G TNA+FN V GDVVRV+LLFYLMPVW +LAR +L+E +T R+ L Sbjct: 71 LWGILIASGATNAAFNWGVTEGDVVRVVLLFYLMPVWATLLARWLLNEKLTRLVGLRLTL 130 Query: 128 GLGGAFIVLYQPRLGLPLPQSLPDWMAVASGLLFAFTNVLLRRMHGLSSDGARAVAMLAG 187 L GA +VL+ GLPLPQ DW+ V G+ FA NVLLR+ + + AR +AM AG Sbjct: 131 ALAGAMVVLWPQGGGLPLPQHPADWLGVIGGMFFALNNVLLRK-YAHTPPEARGLAMFAG 189 Query: 188 SGLLATLLGLLLSLLGMIAWPLQVSAAAAPVVGVWALLFLFSNLCLQYGVSRLPANITAV 247 ++A LL+ G +A + V + AL FL +NL LQYG RLP+ +TAV Sbjct: 190 GVIVAGGAAALLTASGAVAPMPAPAPGWLMGVVLMALAFLAANLGLQYGAERLPSTLTAV 249 Query: 248 VMLTEILVASLSSWALGAAELRVQDLVGG 276 VM EI+ AS S+ LG L Q L GG Sbjct: 250 VMTVEIVFASGSAVLLGVERLSAQALWGG 278 >ref|YP_002942156.1| hypothetical protein Vapar_0227 [Variovorax paradoxus S110] gb|ACS16890.1| protein of unknown function DUF6 transmembrane [Variovorax paradoxus S110] Length = 296 Score = 171 bits (434), Expect = 1e-40, Method: Composition-based stats. Identities = 121/272 (44%), Positives = 165/272 (60%), Gaps = 1/272 (0%) Query: 11 ALLLNTFIWGLSWTGTRALEGMGLHPVWATAAIFSGCALLLLTLRHRELPLLWRHPALLG 70 AL+ N F+WG+SW R +E GLHPVW T I+ +L + R +P L+ Sbjct: 5 ALIFNAFVWGVSWFPFRHIESHGLHPVWTTCIIYVAISLAMGFFRRHAWRGFAAYPMLVL 64 Query: 71 VGLATGLTNASFNMAVAYGDVVRVILLFYLMPVWTVILARLVLHEAITPRSLARVALGLG 130 +GLA G TN FN AV GDVVRV+LLFYLMP+W+V+L VL E T +LARVAL L Sbjct: 65 LGLAAGFTNLGFNWAVTQGDVVRVVLLFYLMPLWSVLLGWAVLGERPTGGALARVALALT 124 Query: 131 GAFIVLYQPRLGLPLPQSLPDWMAVASGLLFAFTNVLLRRMHGLSSDGARAVAMLAGSGL 190 G +VL P + P+P SLPDW+ +A+G FA TN++LR + + +RA+AM G Sbjct: 125 GVAVVLKTPGVEWPVPSSLPDWLGLAAGFSFAVTNIVLRHLRDAPGE-SRALAMFCGCAA 183 Query: 191 LATLLGLLLSLLGMIAWPLQVSAAAAPVVGVWALLFLFSNLCLQYGVSRLPANITAVVML 250 +A + +LLG PL + F+ +N+CLQYG +RL A+ TAV+ML Sbjct: 184 VAGIAAAAGTLLGDFGSPLAAEPGWIGWAALLGTGFVLANICLQYGAARLAASATAVIML 243 Query: 251 TEILVASLSSWALGAAELRVQDLVGGVLIIAA 282 +E+L AS+SS ALGAA++ + L GG LI+ A Sbjct: 244 SEVLFASVSSVALGAAQMDPRILAGGALIVLA 275