BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.5268.p (447 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777512.1| phenylacetyl-CoA-ligase [Herbaspirillum sero... 782 0.0 ref|YP_002008252.1| phenylacetyl-CoA ligase, phenylacetic acid d... 738 0.0 ref|YP_004681511.1| phenylacetate-coenzyme A ligase PaaK [Cupria... 732 0.0 ref|YP_297943.1| phenylacetate-CoA ligase [Ralstonia eutropha JM... 729 0.0 ref|YP_840876.1| phenylacetate-CoA ligase [Ralstonia eutropha H1... 729 0.0 >ref|YP_003777512.1| phenylacetyl-CoA-ligase [Herbaspirillum seropedicae SmR1] gb|ADJ65604.1| phenylacetyl-CoA-ligase protein [Herbaspirillum seropedicae SmR1] Length = 446 Score = 782 bits (2019), Expect = 0.0, Method: Composition-based stats. Identities = 387/437 (88%), Positives = 413/437 (94%), Gaps = 2/437 (0%) Query: 1 MTTRTPQPGDLEPIETASRDELQALQLQRLKWSLQHAYDNVAHYRNAFDQAGVHPDDLKS 60 MT R PQPG+LEPIETASRDELQALQLQRLKWSL+HAYDNV HYRNAFDQAGVHPDDLKS Sbjct: 1 MTIRIPQPGELEPIETASRDELQALQLQRLKWSLRHAYDNVPHYRNAFDQAGVHPDDLKS 60 Query: 61 LSDLSKFPFTTKQDLRDHYPFGMFAVPREQVVRVHASSGTTGKPTVVGYTQNDIDTWATV 120 LSDLS+FPFTTK+DLRDHYPFGMFAVPREQVVRVHASSGTTGKPTVVGYTQNDIDTWATV Sbjct: 61 LSDLSRFPFTTKKDLRDHYPFGMFAVPREQVVRVHASSGTTGKPTVVGYTQNDIDTWATV 120 Query: 121 VARSIRAAGGRRGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDF 180 VARSIRAAGGRRGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDF Sbjct: 121 VARSIRAAGGRRGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDF 180 Query: 181 KPSIIMVTPSYMLNVIEEFQRQGLDAAASSLKVGIFGAEPWTDAMRREIQQRSGIDAVDI 240 KPSIIMVTPSYMLNVIEEFQRQG+D A+SSLKVGIFGAEPWTDAMRREIQQR+G+DAVDI Sbjct: 181 KPSIIMVTPSYMLNVIEEFQRQGMDPASSSLKVGIFGAEPWTDAMRREIQQRAGLDAVDI 240 Query: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIIDPDTGEVLPDGEEGELVFTSLTKEAL 300 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIIDPDTGEVLPDG EGELVFTSL+KEAL Sbjct: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIIDPDTGEVLPDGVEGELVFTSLSKEAL 300 Query: 301 PMIRYRTRDLTRLLPPTSRAMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQLAPQ 360 PMIRYRTRDLTRLLPPTSR+MRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQLAPQ Sbjct: 301 PMIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQLAPQ 360 Query: 361 YQLVVGRNGHLDTLGVTVELRPEVALSAGDIDALARELQHHIKTHVGVTTAVQVVASGKI 420 YQL + R+GH+D++ V ELR +V S ++AL +ELQH IKT+VG++T V VV +G I Sbjct: 361 YQLQIERHGHMDSMSVFAELREDV--SNEHVEALEKELQHSIKTYVGISTKVTVVPAGMI 418 Query: 421 ERSAVGKAKRVVDQRPK 437 ER++VGKAKRV+D R K Sbjct: 419 ERTSVGKAKRVIDNRKK 435 >ref|YP_002008252.1| phenylacetyl-CoA ligase, phenylacetic acid degradation [Cupriavidus taiwanensis LMG 19424] emb|CAQ72198.1| phenylacetyl-CoA ligase, phenylacetic acid degradation [Cupriavidus taiwanensis LMG 19424] Length = 449 Score = 738 bits (1905), Expect = 0.0, Method: Composition-based stats. Identities = 371/449 (82%), Positives = 398/449 (88%), Gaps = 2/449 (0%) Query: 1 MTTRTPQPGDLEPIETASRDELQALQLQRLKWSLQHAYDNVAHYRNAFDQAGVHPDDLKS 60 M R P P +LEPIE ASRDELQALQLQRLKWS++HAYDNV HYR AF AGVHPDDLKS Sbjct: 1 MVQRNPNPNELEPIERASRDELQALQLQRLKWSVRHAYDNVPHYRKAFQVAGVHPDDLKS 60 Query: 61 LSDLSKFPFTTKQDLRDHYPFGMFAVPREQVVRVHASSGTTGKPTVVGYTQNDIDTWATV 120 LSDLSKFPF TKQDLRD+YPFGMFAVPREQV RVHASSGTTGKPTVVGYT DIDTWA+V Sbjct: 61 LSDLSKFPFLTKQDLRDNYPFGMFAVPREQVTRVHASSGTTGKPTVVGYTAKDIDTWASV 120 Query: 121 VARSIRAAGGRRGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDF 180 VARSIRAAGGR GD+V +SYGYGLFTGGLGAHYGAEK GCTVIPMSGGQTEKQVQLIR+F Sbjct: 121 VARSIRAAGGRAGDLVHVSYGYGLFTGGLGAHYGAEKAGCTVIPMSGGQTEKQVQLIREF 180 Query: 181 KPSIIMVTPSYMLNVIEEFQRQGLDAAASSLKVGIFGAEPWTDAMRREIQQRSGIDAVDI 240 +P IIMVTPSYMLN+IEE +RQG+D + SSLKVGIFGAEPWTDAMR EI+ R+GIDAVDI Sbjct: 181 QPDIIMVTPSYMLNLIEEMERQGMDPSESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI 240 Query: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIIDPDTGEVLPDGEEGELVFTSLTKEAL 300 YGLSEVMGPGVA ECIESKDGPVIWEDHFY EIIDP TGE+LPDG EGELVFTSLTKEAL Sbjct: 241 YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEILPDGSEGELVFTSLTKEAL 300 Query: 301 PMIRYRTRDLTRLLPPTSRAMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQLAPQ 360 P+IRYRTRDLTRLLPPTSR+MRRIGKITGRSDDMLIIRGVNVFP+QIEELILK P LAPQ Sbjct: 301 PVIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPSQIEELILKAPALAPQ 360 Query: 361 YQLVVGRNGHLDTLGVTVELRPE--VALSAGDIDALARELQHHIKTHVGVTTAVQVVASG 418 YQLVV R+GHLD L V VE RPE +LSA D AL R+L+ IKT+VGVTT VQVVA+ Sbjct: 361 YQLVVTRDGHLDKLEVRVEARPERSASLSADDRAALERDLKDQIKTYVGVTTRVQVVAAE 420 Query: 419 KIERSAVGKAKRVVDQRPKVVHEAPAGAI 447 IER+ VGKA+RVVD RPKV HE P GAI Sbjct: 421 GIERTTVGKARRVVDMRPKVTHEQPVGAI 449 >ref|YP_004681511.1| phenylacetate-coenzyme A ligase PaaK [Cupriavidus necator N-1] gb|AEI80279.1| phenylacetate-coenzyme A ligase PaaK [Cupriavidus necator N-1] Length = 449 Score = 732 bits (1889), Expect = 0.0, Method: Composition-based stats. Identities = 367/449 (81%), Positives = 396/449 (88%), Gaps = 2/449 (0%) Query: 1 MTTRTPQPGDLEPIETASRDELQALQLQRLKWSLQHAYDNVAHYRNAFDQAGVHPDDLKS 60 M R P P +LEPIE ASRDELQALQL+RLKWS++HAYDNV HYR AF AGVHPDDL+S Sbjct: 1 MVQRIPNPNELEPIERASRDELQALQLERLKWSVRHAYDNVPHYRKAFHAAGVHPDDLQS 60 Query: 61 LSDLSKFPFTTKQDLRDHYPFGMFAVPREQVVRVHASSGTTGKPTVVGYTQNDIDTWATV 120 LSDLSKFPF TKQDLRD+YPFGMFAVPRE+V RVHASSGTTGKPTVVGYT DIDTWA+V Sbjct: 61 LSDLSKFPFLTKQDLRDNYPFGMFAVPREKVARVHASSGTTGKPTVVGYTAKDIDTWASV 120 Query: 121 VARSIRAAGGRRGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDF 180 VARSIRAAGGR GD+V +SYGYGLFTGGLGAHYGAEK GCTVIPMSGGQTEKQVQLIR+F Sbjct: 121 VARSIRAAGGRAGDLVHVSYGYGLFTGGLGAHYGAEKAGCTVIPMSGGQTEKQVQLIREF 180 Query: 181 KPSIIMVTPSYMLNVIEEFQRQGLDAAASSLKVGIFGAEPWTDAMRREIQQRSGIDAVDI 240 +P+IIMVTPSYMLN+IEE +RQG+D + SSLKVGIFGAEPWTDAMR EI+ R+GIDAVDI Sbjct: 181 QPNIIMVTPSYMLNLIEEMERQGMDPSESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI 240 Query: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIIDPDTGEVLPDGEEGELVFTSLTKEAL 300 YGLSEVMGPGVA ECIESKDGPVIWEDHFY EIIDP TGEVLPDGEEGELVFTSLTKEAL Sbjct: 241 YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEVLPDGEEGELVFTSLTKEAL 300 Query: 301 PMIRYRTRDLTRLLPPTSRAMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQLAPQ 360 P+IRYRTRDLTRLLPPTSR+MRRIGKITGRSDDMLIIRGVNVFP+QIEELILK P LAPQ Sbjct: 301 PVIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPSQIEELILKTPALAPQ 360 Query: 361 YQLVVGRNGHLDTLGVTVELR--PEVALSAGDIDALARELQHHIKTHVGVTTAVQVVASG 418 YQLVV R+GHLD L V VE R P AL A D L REL+ IKT+VGVTT VQVV + Sbjct: 361 YQLVVTRDGHLDKLEVRVEARPGPSAALCADDRATLERELKAQIKTYVGVTTRVQVVDAD 420 Query: 419 KIERSAVGKAKRVVDQRPKVVHEAPAGAI 447 IER+ VGKA+RV+D RPKV HE P GAI Sbjct: 421 GIERTTVGKARRVLDMRPKVTHEQPVGAI 449 >ref|YP_297943.1| phenylacetate-CoA ligase [Ralstonia eutropha JMP134] gb|AAZ63099.1| phenylacetate-CoA ligase [Ralstonia eutropha JMP134] Length = 448 Score = 729 bits (1881), Expect = 0.0, Method: Composition-based stats. Identities = 365/448 (81%), Positives = 398/448 (88%), Gaps = 1/448 (0%) Query: 1 MTTRTPQPGDLEPIETASRDELQALQLQRLKWSLQHAYDNVAHYRNAFDQAGVHPDDLKS 60 M RT P +LEPIE ASRDELQALQL+RLKWS++HAYDNV HYR +FD AGVHPDDLKS Sbjct: 1 MVQRTSHPDELEPIERASRDELQALQLERLKWSVRHAYDNVPHYRKSFDAAGVHPDDLKS 60 Query: 61 LSDLSKFPFTTKQDLRDHYPFGMFAVPREQVVRVHASSGTTGKPTVVGYTQNDIDTWATV 120 LSDLSKFPF KQDLRD+YPFGMFAVPRE+V RVHASSGTTGKPTVVGYT DIDTWA+V Sbjct: 61 LSDLSKFPFLAKQDLRDNYPFGMFAVPREKVARVHASSGTTGKPTVVGYTARDIDTWASV 120 Query: 121 VARSIRAAGGRRGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDF 180 VARSIRAAGGR GD+V +SYGYGLFTGGLGAHYGAEK GCTVIPMSGGQTEKQVQLIR+F Sbjct: 121 VARSIRAAGGRPGDLVHVSYGYGLFTGGLGAHYGAEKAGCTVIPMSGGQTEKQVQLIREF 180 Query: 181 KPSIIMVTPSYMLNVIEEFQRQGLDAAASSLKVGIFGAEPWTDAMRREIQQRSGIDAVDI 240 +P+IIMVTPSYMLN+IEE RQG+D A SSLKVGIFGAEPWTDAMR EI+ R+GIDAVDI Sbjct: 181 QPNIIMVTPSYMLNLIEEMMRQGMDPAESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI 240 Query: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIIDPDTGEVLPDGEEGELVFTSLTKEAL 300 YGLSEVMGPGVA ECIESKDGPVIWEDHFY EIIDP TGEVLPDGEEGELVFTSLTKEAL Sbjct: 241 YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEVLPDGEEGELVFTSLTKEAL 300 Query: 301 PMIRYRTRDLTRLLPPTSRAMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQLAPQ 360 P+IRYRTRDLTRLLPPTSR+MRRIGKITGRSDDMLIIRGVNVFP+QIEELILK P LAPQ Sbjct: 301 PVIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPSQIEELILKAPALAPQ 360 Query: 361 YQLVVGRNGHLDTLGVTVELRPEVA-LSAGDIDALARELQHHIKTHVGVTTAVQVVASGK 419 YQLVV R+GHLD L V VE RP+ + LS D AL ++L+ IKT+VGVTT VQV+ + Sbjct: 361 YQLVVTRDGHLDKLEVRVEARPQSSGLSGNDRAALEQQLKEQIKTYVGVTTRVQVLDAEG 420 Query: 420 IERSAVGKAKRVVDQRPKVVHEAPAGAI 447 IER++VGKA+RV+D RPKV HE P GAI Sbjct: 421 IERTSVGKARRVLDMRPKVTHEQPVGAI 448 >ref|YP_840876.1| phenylacetate-CoA ligase [Ralstonia eutropha H16] emb|CAJ96146.1| Phenylacetate-CoA ligase [Ralstonia eutropha H16] Length = 449 Score = 729 bits (1881), Expect = 0.0, Method: Composition-based stats. Identities = 365/449 (81%), Positives = 394/449 (87%), Gaps = 2/449 (0%) Query: 1 MTTRTPQPGDLEPIETASRDELQALQLQRLKWSLQHAYDNVAHYRNAFDQAGVHPDDLKS 60 M R P P +LEPIE ASRDELQALQL+RLKWS++HAYDNV HYR AF AGVHPDDL+S Sbjct: 1 MVQRIPNPNELEPIERASRDELQALQLERLKWSVRHAYDNVPHYRKAFHAAGVHPDDLQS 60 Query: 61 LSDLSKFPFTTKQDLRDHYPFGMFAVPREQVVRVHASSGTTGKPTVVGYTQNDIDTWATV 120 LSDLSKFPF TKQDLRD+YPFGMFAVPRE+V RVHASSGTTGKPTVVGYT DIDTWA+V Sbjct: 61 LSDLSKFPFLTKQDLRDNYPFGMFAVPREKVARVHASSGTTGKPTVVGYTAKDIDTWASV 120 Query: 121 VARSIRAAGGRRGDIVQISYGYGLFTGGLGAHYGAEKLGCTVIPMSGGQTEKQVQLIRDF 180 VARSIRAAGGR GD+V +SYGYGLFTGGLGAHYGAEK GCTVIPMSGGQTEKQVQLIR+F Sbjct: 121 VARSIRAAGGRAGDLVHVSYGYGLFTGGLGAHYGAEKAGCTVIPMSGGQTEKQVQLIREF 180 Query: 181 KPSIIMVTPSYMLNVIEEFQRQGLDAAASSLKVGIFGAEPWTDAMRREIQQRSGIDAVDI 240 +P+IIMVTPSYMLN+IEE +RQG+D + SSLKVGIFGAEPWTDAMR EI+ R+GIDAVDI Sbjct: 181 EPNIIMVTPSYMLNLIEEMERQGMDPSESSLKVGIFGAEPWTDAMRAEIEARAGIDAVDI 240 Query: 241 YGLSEVMGPGVASECIESKDGPVIWEDHFYPEIIDPDTGEVLPDGEEGELVFTSLTKEAL 300 YGLSEVMGPGVA ECIESKDGPVIWEDHFY EIIDP TGEVLPDGEEGELVFTSLTKEAL Sbjct: 241 YGLSEVMGPGVACECIESKDGPVIWEDHFYAEIIDPVTGEVLPDGEEGELVFTSLTKEAL 300 Query: 301 PMIRYRTRDLTRLLPPTSRAMRRIGKITGRSDDMLIIRGVNVFPTQIEELILKLPQLAPQ 360 P+IRYRTRDLTRLLPPTSR+MRRIGKITGRSDDMLIIRGVNVFP+QIEELILK P LAPQ Sbjct: 301 PVIRYRTRDLTRLLPPTSRSMRRIGKITGRSDDMLIIRGVNVFPSQIEELILKTPALAPQ 360 Query: 361 YQLVVGRNGHLDTLGVTVELRP--EVALSAGDIDALARELQHHIKTHVGVTTAVQVVASG 418 YQLVV R+GHLD L V VE RP L A D L REL+ IKT+VGVTT VQVV + Sbjct: 361 YQLVVTRDGHLDKLEVRVEARPGGSAPLCADDRAMLERELKDQIKTYVGVTTRVQVVDAD 420 Query: 419 KIERSAVGKAKRVVDQRPKVVHEAPAGAI 447 IER+ VGKA+RV+D RPK HE P GAI Sbjct: 421 GIERTTVGKARRVLDMRPKFTHEQPVGAI 449