BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.5173.p (264 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777385.1| enoyl-CoA hydratase/carnithine racemase [Her... 462 e-128 ref|YP_001203115.1| enoyl-CoA hydratase [Bradyrhizobium sp. ORS ... 316 3e-84 ref|YP_001242882.1| enoyl-CoA hydratase [Bradyrhizobium sp. BTAi... 314 1e-83 ref|YP_004619617.1| unsaturated acyl-CoA hydratase [Ramlibacter ... 313 1e-83 ref|YP_001790030.1| enoyl-CoA hydratase [Leptothrix cholodnii SP... 313 2e-83 >ref|YP_003777385.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] gb|ADJ65477.1| enoyl-CoA hydratase/carnithine racemase protein [Herbaspirillum seropedicae SmR1] Length = 259 Score = 462 bits (1189), Expect = e-128, Method: Composition-based stats. Identities = 240/258 (93%), Positives = 248/258 (96%) Query: 6 MNQHSDLLDIQVHGAIAEIALNRAAKRNALNDPLVRAIRDCFQDLPEGVKVAIIHGIGEH 65 MNQHS+LLDIQ HGAIAEI LNRAAKRNALNDPLVRAIRDCF++LPEGVKVAI+ G GEH Sbjct: 1 MNQHSELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEH 60 Query: 66 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELASAAHI 125 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELA+AAHI Sbjct: 61 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHI 120 Query: 126 RVAEPSAFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLTAEEGHQAGISQY 185 RVAEPS FYGLPEGQRGLFVGGGGSVRISRLIGVARM DMMLTGRVL+AEEGHQAGI QY Sbjct: 121 RVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQY 180 Query: 186 SVAAGTGLDKARELAERIARNAPMTNYGIMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD 245 SV AG G+DKARELAERIARNAPMTNYG+MHVLPRIHDQSIQDGLMTESLMAAVAGSDPD Sbjct: 181 SVDAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD 240 Query: 246 TKGRLAAFLDHKQNKVKP 263 TKGRLAAFLD KQNKVKP Sbjct: 241 TKGRLAAFLDLKQNKVKP 258 >ref|YP_001203115.1| enoyl-CoA hydratase [Bradyrhizobium sp. ORS 278] emb|CAL74878.1| putative 3-hydroxybutyryl-CoA dehydratase [Bradyrhizobium sp. ORS 278] Length = 266 Score = 316 bits (809), Expect = 3e-84, Method: Composition-based stats. Identities = 160/253 (63%), Positives = 193/253 (76%) Query: 12 LLDIQVHGAIAEIALNRAAKRNALNDPLVRAIRDCFQDLPEGVKVAIIHGIGEHFCAGLD 71 LL I+ GA+ I LNR AKRNALND ++ AI +CF LP+ ++ +IHG+G++F +GLD Sbjct: 14 LLQIEQQGAVLTIGLNRPAKRNALNDGIILAIGECFTSLPDDIRCVVIHGLGDNFSSGLD 73 Query: 72 LSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELASAAHIRVAEPS 131 LSELRERD TE L HS+MWH F++I + RVPVIA L GAVIGGGLELA AAHIRVAEPS Sbjct: 74 LSELRERDATEGLVHSQMWHRVFDRIQYCRVPVIAALKGAVIGGGLELACAAHIRVAEPS 133 Query: 132 AFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLTAEEGHQAGISQYSVAAGT 191 A++ LPEGQRG+FVGGGGSVR+ RLIGVARM DMMLTGRV +A EG G +QY V AG Sbjct: 134 AYFALPEGQRGIFVGGGGSVRLPRLIGVARMMDMMLTGRVYSATEGSSYGFAQYLVEAGA 193 Query: 192 GLDKARELAERIARNAPMTNYGIMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRLA 251 KA ELA +IA NAP+TN+ ++ LP I + + Q GL+ ESLMA VA SD + KGR+ Sbjct: 194 ATTKALELAAKIANNAPLTNFAVLQALPMIAEANPQTGLLMESLMATVAQSDKEAKGRIR 253 Query: 252 AFLDHKQNKVKPT 264 AFLDHK KVKPT Sbjct: 254 AFLDHKTAKVKPT 266 >ref|YP_001242882.1| enoyl-CoA hydratase [Bradyrhizobium sp. BTAi1] gb|ABQ38976.1| trans-feruloyl-CoA hydratase / vanillin synthase [Bradyrhizobium sp. BTAi1] Length = 267 Score = 314 bits (804), Expect = 1e-83, Method: Composition-based stats. Identities = 162/253 (64%), Positives = 194/253 (76%) Query: 12 LLDIQVHGAIAEIALNRAAKRNALNDPLVRAIRDCFQDLPEGVKVAIIHGIGEHFCAGLD 71 LL I+ GA+ I LNR AKRNALND ++ AI DCF LP+ V+V +IHG+G++F +GLD Sbjct: 15 LLQIEQQGAVLTIGLNRPAKRNALNDGIILAIGDCFAGLPDDVRVVVIHGLGDNFSSGLD 74 Query: 72 LSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELASAAHIRVAEPS 131 LSELRERD TE L HS+MWH F++I + RVPVIA L GAVIGGGLELA AAHIRVAEPS Sbjct: 75 LSELRERDATEGLVHSQMWHRVFDRIQYCRVPVIAALKGAVIGGGLELACAAHIRVAEPS 134 Query: 132 AFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLTAEEGHQAGISQYSVAAGT 191 A++ LPEGQRG+FVGGGGSVR+ RLIGVARM DMMLTGRV +A EG G +QY V AG Sbjct: 135 AYFALPEGQRGIFVGGGGSVRLPRLIGVARMMDMMLTGRVYSATEGSSYGFAQYLVEAGA 194 Query: 192 GLDKARELAERIARNAPMTNYGIMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRLA 251 + KA ELA +IA NAP+TN+ ++ LP I + + Q GL+ ESLMA VA SD + K R+ Sbjct: 195 SMTKAMELAGKIASNAPLTNFAVLQALPMIAEANPQTGLLMESLMATVAQSDQEAKRRIR 254 Query: 252 AFLDHKQNKVKPT 264 AFLDHK KVKPT Sbjct: 255 AFLDHKTAKVKPT 267 >ref|YP_004619617.1| unsaturated acyl-CoA hydratase [Ramlibacter tataouinensis TTB310] gb|AEG93598.1| Unsaturated acyl-CoA hydratase [Ramlibacter tataouinensis TTB310] Length = 278 Score = 313 bits (803), Expect = 1e-83, Method: Composition-based stats. Identities = 162/259 (62%), Positives = 199/259 (76%), Gaps = 1/259 (0%) Query: 4 DSMNQHSDLLDIQVHGAIAEIALNRAAKRNALNDPLVRAIRDCFQDLPEGVKVAIIHGIG 63 +SM DL DI + GA+A I LNR AKRNAL+D LV A+RD F++LP V+ A++ G G Sbjct: 16 NSMTPSPDL-DIDIRGAVAVIRLNRPAKRNALSDALVLAVRDAFENLPATVRAAVLGGSG 74 Query: 64 EHFCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELASAA 123 EHFCAGLDLSEL+ERD LHHSRMWHAA E++ +G VPV+A LHGAV+GGGLELA+A Sbjct: 75 EHFCAGLDLSELKERDAGAGLHHSRMWHAALERVQYGPVPVVAALHGAVVGGGLELAAAC 134 Query: 124 HIRVAEPSAFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLTAEEGHQAGIS 183 HIRVA+ +AFY LPEG RG+FVGGGG+VRI RLIG ARM DMMLTGRV AEEG +AG + Sbjct: 135 HIRVADDTAFYALPEGSRGIFVGGGGAVRIPRLIGAARMTDMMLTGRVYNAEEGERAGFA 194 Query: 184 QYSVAAGTGLDKARELAERIARNAPMTNYGIMHVLPRIHDQSIQDGLMTESLMAAVAGSD 243 QY V AG DKA LA+R+A NAP+TNY ++H LPRI +Q G +TE+LMAA+A S Sbjct: 195 QYRVPAGQAFDKALALAQRVAENAPLTNYALVHALPRIAEQPADQGFLTEALMAAIAQSA 254 Query: 244 PDTKGRLAAFLDHKQNKVK 262 P+ K R+ AFL+ + +KVK Sbjct: 255 PEAKARVRAFLEGRADKVK 273 >ref|YP_001790030.1| enoyl-CoA hydratase [Leptothrix cholodnii SP-6] gb|ACB33265.1| Enoyl-CoA hydratase/isomerase [Leptothrix cholodnii SP-6] Length = 273 Score = 313 bits (801), Expect = 2e-83, Method: Composition-based stats. Identities = 159/251 (63%), Positives = 193/251 (76%) Query: 11 DLLDIQVHGAIAEIALNRAAKRNALNDPLVRAIRDCFQDLPEGVKVAIIHGIGEHFCAGL 70 DLL I +G + I +NR AKRNAL+D L++ + F +LP+GV A+I G GEHFCAGL Sbjct: 20 DLLQISCNGKVLHICINRPAKRNALSDTLIQQLHTAFVNLPDGVAAAVISGAGEHFCAGL 79 Query: 71 DLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELASAAHIRVAEP 130 DLSEL ERD + HSRMWHAAF+ I FG+VPV+A LHGAV+GGGLELASA H+RVA+ Sbjct: 80 DLSELVERDIGSGVKHSRMWHAAFDLIQFGQVPVVAALHGAVVGGGLELASACHVRVADE 139 Query: 131 SAFYGLPEGQRGLFVGGGGSVRISRLIGVARMADMMLTGRVLTAEEGHQAGISQYSVAAG 190 S ++ LPEGQRG+FVGGGGSVRI RL+GVARM D+MLTGRV A EG AG++QY V G Sbjct: 140 STYFALPEGQRGIFVGGGGSVRIPRLMGVARMTDLMLTGRVYDAAEGVAAGLAQYLVPQG 199 Query: 191 TGLDKARELAERIARNAPMTNYGIMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTKGRL 250 T +D+A LA+RIA NAP++NY +MH LPRI DQS+ DGL TESLMAAVA S PD + RL Sbjct: 200 TSVDRAIALAQRIATNAPLSNYAVMHALPRIADQSMSDGLFTESLMAAVAESTPDAQDRL 259 Query: 251 AAFLDHKQNKV 261 AFL+ + KV Sbjct: 260 RAFLEKRAGKV 270