BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.5158.p (391 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777371.1| vanillate O-demethylase oxygenase subunit ox... 701 0.0 gb|ACP17982.1| putative vanillate-O-demethylase oxidoreductase a... 623 e-176 sp|O05616|VANA_PSEUH RecName: Full=Vanillate O-demethylase oxyge... 609 e-172 ref|YP_004476099.1| vanillate monooxygenase [Pseudomonas fulva 1... 600 e-169 ref|YP_004354294.1| Vanillate monooxygenase [Pseudomonas brassic... 595 e-168 >ref|YP_003777371.1| vanillate O-demethylase oxygenase subunit oxidoreductase [Herbaspirillum seropedicae SmR1] gb|ADJ65463.1| vanillate O-demethylase oxygenase subunit oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 701 bits (1810), Expect = 0.0, Method: Composition-based stats. Identities = 329/349 (94%), Positives = 341/349 (97%) Query: 43 MFPKNAWYVACTPDEIESKPLGRQICGEKIVFYRGAEGKVAAVEDFCPHRGAPLSLGFVR 102 MFPKNAWYVACTPDEI+SKPLGRQICGEKIVFYRGA GKVAAVEDFCPHRGAPLSLGFVR Sbjct: 1 MFPKNAWYVACTPDEIDSKPLGRQICGEKIVFYRGAGGKVAAVEDFCPHRGAPLSLGFVR 60 Query: 103 EGQLVCGYHGLEMGCDGQTRSMPGQRVRGFPCIRSFPVVERYGFVWVWPGDKEQADPAQI 162 EGQLVCGYHGLEMGCDG+ SMPGQRVRGFPCIRS+PV ERYGF+WVWPGDKEQADPAQI Sbjct: 61 EGQLVCGYHGLEMGCDGKAISMPGQRVRGFPCIRSYPVEERYGFIWVWPGDKEQADPAQI 120 Query: 163 HHLEWAENPEWAYGGGLYHINCDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPVNTRVE 222 HHLEWA+NPEWAYGGGLYHINCDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPVNTRVE Sbjct: 121 HHLEWADNPEWAYGGGLYHINCDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPVNTRVE 180 Query: 223 GEQVITSRFMENIMPPPFWRMALRGNGLADEVPVDRWQICRFSPPSHVMIEVGVAHAGKG 282 GEQVITSRFM+NIMPPPFWRMALRGNGLAD+VPVDRWQICRFSPPSHVMIEVGVAHAG G Sbjct: 181 GEQVITSRFMDNIMPPPFWRMALRGNGLADDVPVDRWQICRFSPPSHVMIEVGVAHAGHG 240 Query: 283 GYEAGPEHKASSIVVDFITPETETSHWYFWGMARHFRPEDKELTNTIREGQGKIFGEDRE 342 GYEAGPEHKASSIVVDFITPETETSHWYFWGMARHFRP+DKELT+TIREGQGKIFGEDRE Sbjct: 241 GYEAGPEHKASSIVVDFITPETETSHWYFWGMARHFRPQDKELTDTIREGQGKIFGEDRE 300 Query: 343 MLELQQANLLKYPERRLLALNIDAGGVQSRKVLDKWLAREAESGKAAAA 391 MLELQQANLL++PERRLLALNIDAGGVQSRKVLDKWLAREA G+ AAA Sbjct: 301 MLELQQANLLRHPERRLLALNIDAGGVQSRKVLDKWLAREAGDGQPAAA 349 >gb|ACP17982.1| putative vanillate-O-demethylase oxidoreductase a-subunit [Pseudomonas nitroreducens] Length = 354 Score = 623 bits (1606), Expect = e-176, Method: Composition-based stats. Identities = 284/347 (81%), Positives = 317/347 (91%) Query: 43 MFPKNAWYVACTPDEIESKPLGRQICGEKIVFYRGAEGKVAAVEDFCPHRGAPLSLGFVR 102 MFPKNAWYVACTPDEI KPLGRQIC EKIVFYRGAEG+VAAVEDFCPHRGAPLSLGFVR Sbjct: 1 MFPKNAWYVACTPDEIADKPLGRQICNEKIVFYRGAEGRVAAVEDFCPHRGAPLSLGFVR 60 Query: 103 EGQLVCGYHGLEMGCDGQTRSMPGQRVRGFPCIRSFPVVERYGFVWVWPGDKEQADPAQI 162 +G+L+CGYHGLEMGC+G+T +MPGQRV+GFPCI+S+ V ERYGF+WVWPGD+E ADPA I Sbjct: 61 DGKLICGYHGLEMGCEGKTLAMPGQRVQGFPCIKSYAVEERYGFIWVWPGDRELADPALI 120 Query: 163 HHLEWAENPEWAYGGGLYHINCDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPVNTRVE 222 HHLEWA+NPEWAYGGGLYHI CDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPV+TRVE Sbjct: 121 HHLEWADNPEWAYGGGLYHIACDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPVSTRVE 180 Query: 223 GEQVITSRFMENIMPPPFWRMALRGNGLADEVPVDRWQICRFSPPSHVMIEVGVAHAGKG 282 G+ VITSR+M+N+M PPFWR ALRGNGLAD+VPVDRWQICRF+PPSHV+IEVGVAHAGKG Sbjct: 181 GDTVITSRYMDNVMAPPFWRAALRGNGLADDVPVDRWQICRFAPPSHVLIEVGVAHAGKG 240 Query: 283 GYEAGPEHKASSIVVDFITPETETSHWYFWGMARHFRPEDKELTNTIREGQGKIFGEDRE 342 GY+A E+KA SIVVDFITPE++TS WYFWGMAR+FRP+D ELT TIR GQGKIF ED + Sbjct: 241 GYDAPAEYKAGSIVVDFITPESDTSIWYFWGMARNFRPQDAELTETIRVGQGKIFAEDLD 300 Query: 343 MLELQQANLLKYPERRLLALNIDAGGVQSRKVLDKWLAREAESGKAA 389 MLE QQ NLL YPER+LL LNIDAGGVQSR+V+D+ LA E E+ AA Sbjct: 301 MLEQQQRNLLAYPERQLLKLNIDAGGVQSRRVIDRILAAEQETADAA 347 >sp|O05616|VANA_PSEUH RecName: Full=Vanillate O-demethylase oxygenase subunit; AltName: Full=4-hydroxy-3-methoxybenzoate demethylase emb|CAA72287.1| a-subunit oxygenase [Pseudomonas sp.] Length = 354 Score = 609 bits (1571), Expect = e-172, Method: Composition-based stats. Identities = 277/337 (82%), Positives = 309/337 (91%) Query: 43 MFPKNAWYVACTPDEIESKPLGRQICGEKIVFYRGAEGKVAAVEDFCPHRGAPLSLGFVR 102 MFPKNAWYVACTPDEI KPLGRQIC EKIVFYRG EG+VAAVEDFCPHRGAPLSLGFVR Sbjct: 1 MFPKNAWYVACTPDEIADKPLGRQICNEKIVFYRGPEGRVAAVEDFCPHRGAPLSLGFVR 60 Query: 103 EGQLVCGYHGLEMGCDGQTRSMPGQRVRGFPCIRSFPVVERYGFVWVWPGDKEQADPAQI 162 +G+L+CGYHGLEMGC+G+T +MPGQRV+GFPCI+S+ V ERYGF+WVWPGD+E ADPA I Sbjct: 61 DGKLICGYHGLEMGCEGKTLAMPGQRVQGFPCIKSYAVEERYGFIWVWPGDRELADPALI 120 Query: 163 HHLEWAENPEWAYGGGLYHINCDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPVNTRVE 222 HHLEWA+NPEWAYGGGLYHI CDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPV+TRVE Sbjct: 121 HHLEWADNPEWAYGGGLYHIACDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPVSTRVE 180 Query: 223 GEQVITSRFMENIMPPPFWRMALRGNGLADEVPVDRWQICRFSPPSHVMIEVGVAHAGKG 282 G+ VITSR+M+N+M PPFWR ALRGNGLAD+VPVDRWQICRF+PPSHV+IEVGVAHAGKG Sbjct: 181 GDTVITSRYMDNVMAPPFWRAALRGNGLADDVPVDRWQICRFAPPSHVLIEVGVAHAGKG 240 Query: 283 GYEAGPEHKASSIVVDFITPETETSHWYFWGMARHFRPEDKELTNTIREGQGKIFGEDRE 342 GY+A E+KA SIVVDFITPE++TS WYFWGMAR+FRP+ ELT TIR GQGKIF ED + Sbjct: 241 GYDAPAEYKAGSIVVDFITPESDTSIWYFWGMARNFRPQGTELTETIRVGQGKIFAEDLD 300 Query: 343 MLELQQANLLKYPERRLLALNIDAGGVQSRKVLDKWL 379 MLE QQ NLL YPER+LL LNIDAGGVQSR+V+D+ L Sbjct: 301 MLEQQQRNLLAYPERQLLKLNIDAGGVQSRRVIDRIL 337 >ref|YP_004476099.1| vanillate monooxygenase [Pseudomonas fulva 12-X] gb|AEF24005.1| Vanillate monooxygenase [Pseudomonas fulva 12-X] Length = 354 Score = 600 bits (1547), Expect = e-169, Method: Composition-based stats. Identities = 278/340 (81%), Positives = 306/340 (90%) Query: 43 MFPKNAWYVACTPDEIESKPLGRQICGEKIVFYRGAEGKVAAVEDFCPHRGAPLSLGFVR 102 M+PKNAWYVACTPDEI KPLGRQICGEKIVFYRGAEG+V A+EDFCPHRGAPLSLG+V Sbjct: 1 MYPKNAWYVACTPDEIAEKPLGRQICGEKIVFYRGAEGQVVALEDFCPHRGAPLSLGYVE 60 Query: 103 EGQLVCGYHGLEMGCDGQTRSMPGQRVRGFPCIRSFPVVERYGFVWVWPGDKEQADPAQI 162 G L+CGYHGLEMGC+G+T SMPGQRVRGFP IR+F VERYGF+WVW GD+ ADPA I Sbjct: 61 NGNLICGYHGLEMGCEGKTVSMPGQRVRGFPGIRAFAAVERYGFIWVWTGDQALADPATI 120 Query: 163 HHLEWAENPEWAYGGGLYHINCDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPVNTRVE 222 HHLEWAENP+WAYGGGL+HINCDYRLMIDNLMDLTHETYVHASSIGQKEIDEAP T+VE Sbjct: 121 HHLEWAENPDWAYGGGLFHINCDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPPVTKVE 180 Query: 223 GEQVITSRFMENIMPPPFWRMALRGNGLADEVPVDRWQICRFSPPSHVMIEVGVAHAGKG 282 G++VITSR MENIM PPFWRMALRGNGLAD+VPVDRWQICRF+PPSHVMIEVGVAHAG G Sbjct: 181 GDEVITSRHMENIMAPPFWRMALRGNGLADDVPVDRWQICRFNPPSHVMIEVGVAHAGHG 240 Query: 283 GYEAGPEHKASSIVVDFITPETETSHWYFWGMARHFRPEDKELTNTIREGQGKIFGEDRE 342 GYEA E KASSIVVDFITPE+ETS WYFWGMAR F+PED+ LT++IR+GQGKIF ED E Sbjct: 241 GYEAPAEVKASSIVVDFITPESETSIWYFWGMARSFKPEDQALTDSIRQGQGKIFSEDLE 300 Query: 343 MLELQQANLLKYPERRLLALNIDAGGVQSRKVLDKWLARE 382 MLE QQ NLL PER+LL LNIDAGGVQSR+++D+ LA E Sbjct: 301 MLERQQRNLLWQPERQLLKLNIDAGGVQSRRIIDRLLAEE 340 >ref|YP_004354294.1| Vanillate monooxygenase [Pseudomonas brassicacearum subsp. brassicacearum NFM421] gb|AEA69290.1| Vanillate monooxygenase [Pseudomonas brassicacearum subsp. brassicacearum NFM421] Length = 361 Score = 595 bits (1533), Expect = e-168, Method: Composition-based stats. Identities = 274/343 (79%), Positives = 304/343 (88%) Query: 43 MFPKNAWYVACTPDEIESKPLGRQICGEKIVFYRGAEGKVAAVEDFCPHRGAPLSLGFVR 102 M+PKNAWYVACTPDEI KPLGRQICGEK+VFYRG EGKVAAVEDFCPHRGAPLSLG V Sbjct: 1 MYPKNAWYVACTPDEIADKPLGRQICGEKMVFYRGHEGKVAAVEDFCPHRGAPLSLGHVE 60 Query: 103 EGQLVCGYHGLEMGCDGQTRSMPGQRVRGFPCIRSFPVVERYGFVWVWPGDKEQADPAQI 162 G LVCGYHGL MGCDG+T MPGQRVRGFPC ++F V ER+GF+WVWPGD+ ADPA I Sbjct: 61 NGNLVCGYHGLVMGCDGKTVEMPGQRVRGFPCNKTFAVQERHGFIWVWPGDQALADPALI 120 Query: 163 HHLEWAENPEWAYGGGLYHINCDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPVNTRVE 222 HHLEWAE+ EWAYGGGL+HI CDYRLMIDNLMDLTHETYVHASSIGQKEIDEAP T V+ Sbjct: 121 HHLEWAESDEWAYGGGLFHIQCDYRLMIDNLMDLTHETYVHASSIGQKEIDEAPPVTTVD 180 Query: 223 GEQVITSRFMENIMPPPFWRMALRGNGLADEVPVDRWQICRFSPPSHVMIEVGVAHAGKG 282 G++V+T+R MENIM PPFWRMALRGN LAD+VPVDRWQICRF+PPSHV+IEVGVAHAG G Sbjct: 181 GDEVVTARHMENIMAPPFWRMALRGNNLADDVPVDRWQICRFTPPSHVLIEVGVAHAGNG 240 Query: 283 GYEAGPEHKASSIVVDFITPETETSHWYFWGMARHFRPEDKELTNTIREGQGKIFGEDRE 342 GY A P+ KASSIVVDFITPETETS WYFWGMARHF+P+D+ LT +IREGQGKIF ED E Sbjct: 241 GYHAAPQFKASSIVVDFITPETETSIWYFWGMARHFQPQDEALTASIREGQGKIFSEDLE 300 Query: 343 MLELQQANLLKYPERRLLALNIDAGGVQSRKVLDKWLAREAES 385 MLE QQ NLL +P+R LL LNIDAGGVQSR+VL++W+ARE E+ Sbjct: 301 MLERQQRNLLDHPQRNLLKLNIDAGGVQSRRVLERWIAREREA 343