BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4968.p (357 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777212.1| glycolate oxidase iron-sulfur subunit oxidor... 639 0.0 ref|YP_004751397.1| glycolate dehydrogenase, FAD-binding subunit... 415 e-114 ref|YP_001352026.1| glycolate oxidase, subunit GlcE [Janthinobac... 393 e-107 ref|YP_004686845.1| 2-hydroxy-acid oxidase, FAD-binding subunit ... 380 e-103 ref|YP_002006563.1| glycolate oxidase fad binding subunit [Cupri... 380 e-103 >ref|YP_003777212.1| glycolate oxidase iron-sulfur subunit oxidoreductase [Herbaspirillum seropedicae SmR1] gb|ADJ65304.1| glycolate oxidase iron-sulfur subunit oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 359 Score = 639 bits (1648), Expect = 0.0, Method: Composition-based stats. Identities = 333/356 (93%), Positives = 345/356 (96%) Query: 2 QAQQDMDAVLASFRERILSGRALQIRGGGTKDWYGQDPCGDLLDTRPYSGIIDYEPTELV 61 QAQQD+DAVLASFRERIL+GR LQIRGGGTKDWYGQ P G+LLDTR YSGIIDYEPTELV Sbjct: 4 QAQQDLDAVLASFRERILAGRPLQIRGGGTKDWYGQTPSGELLDTRAYSGIIDYEPTELV 63 Query: 62 ITARCGTSLAEIDAALAQHNQMLAWEPPRFGGAATVGGMVAAGLSGPSRASAGAVRDFVL 121 ITARCGT L+EIDAALAQHNQMLAWEPP FGGAATVGGMVAAGLSGP+RASAGAVRDFVL Sbjct: 64 ITARCGTPLSEIDAALAQHNQMLAWEPPSFGGAATVGGMVAAGLSGPARASAGAVRDFVL 123 Query: 122 GAVLMDAQGEQLHFGGQVMKNVAGYDISRLLAGSLGTLGLILQVSLKVLPRPIASSTRVF 181 GAVLMDAQGE LHFGGQVMKNVAGYDISRLLAGSLGTLGLIL+VSLKVLPRPIASSTRVF Sbjct: 124 GAVLMDAQGEPLHFGGQVMKNVAGYDISRLLAGSLGTLGLILEVSLKVLPRPIASSTRVF 183 Query: 182 EINQEGALRLLNQSAGQPLPLVASAWVNNVLTIRLSGAQAAVDSAIKKMGGTELSNPEDY 241 EINQEGALRLLNQSAGQPLPLVASAWV NVLTIRLSGAQAAVDSAI K+GGTELSNP+DY Sbjct: 184 EINQEGALRLLNQSAGQPLPLVASAWVGNVLTIRLSGAQAAVDSAIAKLGGTELSNPDDY 243 Query: 242 WTDLREQTHPFFADSQAPLWRLSLPSVAPPVDLPGRQLIEWGGAQRWLRPEGNVDAVAIR 301 WTDLREQTHPFFAD++APLWRLSLPSVA P+DLPGRQLIEWGGAQRWLRPEGNVDAV IR Sbjct: 244 WTDLREQTHPFFADAEAPLWRLSLPSVAAPIDLPGRQLIEWGGAQRWLRPEGNVDAVTIR 303 Query: 302 AAALAVGGHASLHRGGDKAIGVFHPLQPAVETIHRRLKQQFDPQGIFNPGRLYASL 357 AAALAVGGHASLHRGGDKAIGVFHPLQPAVET+HRRLKQQFDPQGIFNPGRLYASL Sbjct: 304 AAALAVGGHASLHRGGDKAIGVFHPLQPAVETLHRRLKQQFDPQGIFNPGRLYASL 359 >ref|YP_004751397.1| glycolate dehydrogenase, FAD-binding subunit [Collimonas fungivorans Ter331] gb|AEK60574.1| Glycolate dehydrogenase, FAD-binding subunit [Collimonas fungivorans Ter331] Length = 370 Score = 415 bits (1066), Expect = e-114, Method: Composition-based stats. Identities = 237/359 (66%), Positives = 281/359 (78%), Gaps = 10/359 (2%) Query: 4 QQDMDAVLAS--FRERILSG----RALQIRGGGTKDWYGQDPCGDLLDTRPYSGIIDYEP 57 Q +MD L +E+IL+ R L+IRGGG+K WYGQ G+LLDTR YSG+IDYEP Sbjct: 11 QNNMDPELFKQLAKEQILAAAAQRRTLEIRGGGSKRWYGQLVQGELLDTRAYSGVIDYEP 70 Query: 58 TELVITARCGTSLAEIDAALAQHNQMLAWEPPRFGGAATVGGMVAAGLSGPSRASAGAVR 117 TELVITARCGT LAEI+A LAQHNQ+L +EPP FG AAT+GGMVA+GLSGPSR + GA+R Sbjct: 71 TELVITARCGTPLAEIEALLAQHNQVLPFEPPHFGSAATLGGMVASGLSGPSRQAVGALR 130 Query: 118 DFVLGAVLMDAQGEQLHFGGQVMKNVAGYDISRLLAGSLGTLGLILQVSLKVLPRPIASS 177 DFVLGAVLMD +G+ LHFGGQVMKNVAGYD+SRLLAGSLGTLGLIL+VSLKVLPRP+ASS Sbjct: 131 DFVLGAVLMDGKGDILHFGGQVMKNVAGYDVSRLLAGSLGTLGLILEVSLKVLPRPLASS 190 Query: 178 TRVFEINQEGALRLLNQSAGQPLPLVASAWVNNVLTIRLSGAQAAVDSAIKKMGGTELSN 237 +R F + + A+R LNQ G PLP+ AS W + LT+RLSGAQAAV +A + +GG +++ Sbjct: 191 SRRFAMGEAEAIRSLNQWGGLPLPISASCWQDGQLTLRLSGAQAAVSAAEQNLGGEAVAD 250 Query: 238 PEDYWTDLREQTHPFFAD--SQAPLWRLSLPSVAPPVDLPGRQLIEWGGAQRWLRPEGNV 295 +D+W LREQTH FFA +Q PLWRL+LPS A P+ LPG LIEWGGAQRWL + Sbjct: 251 ADDFWQALREQTHAFFAPAAAQKPLWRLALPSTAAPLALPGAVLIEWGGAQRWLFTD--T 308 Query: 296 DAVAIRAAALAVGGHASLHRGGDKAIGVFHPLQPAVETIHRRLKQQFDPQGIFNPGRLY 354 DA AIR+AA A GGHA+L+RGGDK GVFHPL PA+ IHRRLK FDP IFN GR+Y Sbjct: 309 DAQAIRSAAAAAGGHATLYRGGDKDAGVFHPLAPALAQIHRRLKASFDPAAIFNIGRMY 367 >ref|YP_001352026.1| glycolate oxidase, subunit GlcE [Janthinobacterium sp. Marseille] gb|ABR88928.1| glycolate oxidase, subunit GlcE [Janthinobacterium sp. Marseille] Length = 351 Score = 393 bits (1010), Expect = e-107, Method: Composition-based stats. Identities = 217/353 (61%), Positives = 273/353 (77%), Gaps = 8/353 (2%) Query: 7 MDAVLASFRERILSG----RALQIRGGGTKDWYGQDPCGDLLDTRPYSGIIDYEPTELVI 62 M+ V+ F+ERIL+ + L+IRGGGTKDWYGQ P G+LLDTR YSG++ Y+PTELVI Sbjct: 1 MEQVIEQFKERILAAASNKQPLRIRGGGTKDWYGQTPVGELLDTRAYSGVVAYDPTELVI 60 Query: 63 TARCGTSLAEIDAALAQHNQMLAWEPPRFGGAATVGGMVAAGLSGPSRASAGAVRDFVLG 122 T R GT LAE++A LA+ NQMLA+EPP FG AT+GGMVA+GLSGP R + GA RDFVLG Sbjct: 61 TVRSGTPLAEVEAILAEQNQMLAFEPPHFGPGATIGGMVASGLSGPGRVAVGATRDFVLG 120 Query: 123 AVLMDAQGEQLHFGGQVMKNVAGYDISRLLAGSLGTLGLILQVSLKVLPRPIASSTRVFE 182 AVLMD +GE LHFGGQVMKNVAGYD+SR+LAGSLGTLGL+L++S+KVLP+P A ++ E Sbjct: 121 AVLMDGKGESLHFGGQVMKNVAGYDVSRMLAGSLGTLGLLLELSVKVLPKPYAQTSLRLE 180 Query: 183 INQEGALRLLNQSAGQPLPLVASAWVNNVLTIRLSGAQAAVDSAIKKMGGTELSNPEDYW 242 ++++ A+R LN+ GQPLP+ ASAW + L +RLSGA+AA+ +A + +GG E+ +D+W Sbjct: 181 MDEKDAIRRLNEWGGQPLPISASAWCDGALMVRLSGAKAALAAAQQTIGGEEVDG-DDFW 239 Query: 243 TDLREQTHPFFADSQAPLWRLSLPSVAPPVDLPGRQLIEWGGAQRWLRPEGNVDAVAIRA 302 +REQ++ FF S LWRLS+PS P+ L G+QLIEWGGAQRWLR N A IR Sbjct: 240 IAMREQSNEFFTQSDV-LWRLSVPSTTAPIALNGQQLIEWGGAQRWLR--SNESAETIRT 296 Query: 303 AALAVGGHASLHRGGDKAIGVFHPLQPAVETIHRRLKQQFDPQGIFNPGRLYA 355 AA GGHA+L RGGDKA+GVFHPL PA+ IH+ +K FDP GIFNPGR+YA Sbjct: 297 AAALAGGHATLFRGGDKAVGVFHPLPPALGRIHQNMKNNFDPDGIFNPGRMYA 349 >ref|YP_004686845.1| 2-hydroxy-acid oxidase, FAD-binding subunit [Cupriavidus necator N-1] gb|AEI78364.1| 2-hydroxy-acid oxidase, FAD-binding subunit [Cupriavidus necator N-1] Length = 367 Score = 380 bits (977), Expect = e-103, Method: Composition-based stats. Identities = 217/366 (59%), Positives = 265/366 (72%), Gaps = 12/366 (3%) Query: 4 QQDMDAVLASFRERILSGRALQIRGGGTKDWYGQDPCGDLLDTRPYSGIIDYEPTELVIT 63 Q +DA+ + R+ + AL++RGGG+KD+YGQ G LLDTR Y+GI++Y+P ELVIT Sbjct: 2 QATLDAIRDAVRQATETRTALRLRGGGSKDFYGQPAAGQLLDTRAYAGIVEYDPAELVIT 61 Query: 64 ARCGTSLAEIDAALAQHNQMLAWEPPRFG-----GAATVGGMVAAGLSGPSRASAGAVRD 118 ARCGT L EI+AALA+ QMLA+EPP F AT+GG VAAGLSGP R S GA+RD Sbjct: 62 ARCGTPLTEIEAALAEKRQMLAFEPPHFALPGQPSTATLGGAVAAGLSGPRRQSVGALRD 121 Query: 119 FVLGAVLMDAQGEQLHFGGQVMKNVAGYDISRLLAGSLGTLGLILQVSLKVLPRPIASST 178 F+LGA LMD +GE + FGGQVMKNVAGYD+SRLLAGSLGTLGLILQVS+KVLP P +T Sbjct: 122 FMLGAQLMDGRGEVMDFGGQVMKNVAGYDVSRLLAGSLGTLGLILQVSVKVLPAPFDEAT 181 Query: 179 RVFEINQEGALRLLNQSAGQPLPLVASAWVNNVLTIRLSGAQAAVDSAIKKMGGTELSNP 238 FE+ Q A+R LNQ GQPLPL +SAW VL +RL+GA AAV +A ++GG + Sbjct: 182 LRFELAQADAIRQLNQWGGQPLPLASSAWHAGVLHVRLAGATAAVRAACARLGGERVDAA 241 Query: 239 E--DYWTDLREQTHPFFADSQA--PLWRLSLPSVAPPVDLPGRQLIEWGGAQRWLRPE-- 292 E W LREQTHPFFA +QA LWRL++P++A P++LPG QLIEWGG QRW PE Sbjct: 242 EAAALWQTLREQTHPFFAPAQAGRALWRLAVPTIAAPLELPGSQLIEWGGGQRWWLPEDG 301 Query: 293 -GNVDAVAIRAAALAVGGHASLHRGGDKAIGVFHPLQPAVETIHRRLKQQFDPQGIFNPG 351 DA ++RA A A GGHA+L R GDK++GVF PL + IHRRLK+ FDP GIFNP Sbjct: 302 TDKTDAESVRAVAQAAGGHATLFRNGDKSVGVFTPLATPLAAIHRRLKETFDPAGIFNPQ 361 Query: 352 RLYASL 357 R+Y L Sbjct: 362 RMYPGL 367 >ref|YP_002006563.1| glycolate oxidase fad binding subunit [Cupriavidus taiwanensis LMG 19424] emb|CAQ70502.1| Glycolate oxidase subunit glcE [Cupriavidus taiwanensis LMG 19424] Length = 367 Score = 380 bits (976), Expect = e-103, Method: Composition-based stats. Identities = 218/366 (59%), Positives = 262/366 (71%), Gaps = 12/366 (3%) Query: 4 QQDMDAVLASFRERILSGRALQIRGGGTKDWYGQDPCGDLLDTRPYSGIIDYEPTELVIT 63 Q +DA + R+ + AL++RGGG+KD+YGQ P G LLDTR Y+GI+DY+P ELVIT Sbjct: 2 QATLDAFRDAVRQATETRTALRLRGGGSKDFYGQPPQGQLLDTRAYTGIVDYDPAELVIT 61 Query: 64 ARCGTSLAEIDAALAQHNQMLAWEPPRFG-----GAATVGGMVAAGLSGPSRASAGAVRD 118 ARCGT LAEI+AALA+ Q+LA+EPP F AT+GG VAAGLSGP R S GA+RD Sbjct: 62 ARCGTPLAEIEAALAEKRQILAFEPPHFALPGRPSVATLGGAVAAGLSGPRRQSVGALRD 121 Query: 119 FVLGAVLMDAQGEQLHFGGQVMKNVAGYDISRLLAGSLGTLGLILQVSLKVLPRPIASST 178 FVLGA LMD +GE + FGGQVMKNVAGYD+SRLL GSLGTLGLILQVS+KVLP P +T Sbjct: 122 FVLGAQLMDGRGEVMDFGGQVMKNVAGYDVSRLLTGSLGTLGLILQVSVKVLPAPFDEAT 181 Query: 179 RVFEINQEGALRLLNQSAGQPLPLVASAWVNNVLTIRLSGAQAAVDSAIKKMGGTEL--S 236 FE+ Q A+R LNQ GQPLPL +S W VL +RLSGA AAV +A ++GG L + Sbjct: 182 LRFEMTQAEAIRQLNQWGGQPLPLASSVWHAGVLHVRLSGASAAVRAACARLGGERLDPA 241 Query: 237 NPEDYWTDLREQTHPFFADSQA--PLWRLSLPSVAPPVDLPGRQLIEWGGAQRWLRPE-- 292 W LREQTHPFFA + A LWRL++P+VA P+ LPG QL+EWGG QRW PE Sbjct: 242 PAAALWQALREQTHPFFAPAHAGRALWRLAVPTVAAPLALPGEQLVEWGGGQRWWLPEEG 301 Query: 293 -GNVDAVAIRAAALAVGGHASLHRGGDKAIGVFHPLQPAVETIHRRLKQQFDPQGIFNPG 351 DA ++RA A A GGHA+L R GDK++GVF PL + IHRRLK+ FDP GIFNP Sbjct: 302 SAKADAESVRAVAQAAGGHATLFRHGDKSMGVFTPLASPLAAIHRRLKETFDPAGIFNPQ 361 Query: 352 RLYASL 357 R+Y L Sbjct: 362 RMYPGL 367