BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4851.p (374 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775893.1| D-isomer specific 2-hydroxyacid dehydrogenas... 395 e-108 ref|YP_001566649.1| NAD-binding D-isomer specific 2-hydroxyacid ... 359 3e-97 ref|YP_004486349.1| hydroxypyruvate reductase [Delftia sp. Cs1-4... 358 1e-96 ref|ZP_08529222.1| 2-hydroxyacid dehydrogenase [Agrobacterium sp... 314 2e-83 ref|NP_396409.2| 2-hydroxyacid dehydrogenase [Agrobacterium tume... 313 2e-83 >ref|YP_003775893.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Herbaspirillum seropedicae SmR1] gb|ADJ63985.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 319 Score = 395 bits (1014), Expect = e-108, Method: Composition-based stats. Identities = 246/312 (78%), Positives = 268/312 (85%) Query: 63 APIPFVSQSDAAAVAAWLQALRAAMPEEEIIAFSELDEDQRAQCRVAIVANPDPAQLKLL 122 +PI FVSQ+ A+ V W+ AL+AAMPEE I+ +LD DQ AQCRVAIVANPDPAQLK + Sbjct: 8 SPIAFVSQAGASEVQQWISALQAAMPEERILPCEQLDADQLAQCRVAIVANPDPAQLKRM 67 Query: 123 PQLQWVHSVWAGVERLVADLGQSDLQITRLVDPRLAATMAEAVLAWTLYLHRDMPHYARQ 182 P L WVHSVWAGVERLVADLGQSDLQ+TRLVDPRLAATMAEAVLAWTLYLHRDMP YAR+ Sbjct: 68 PNLLWVHSVWAGVERLVADLGQSDLQLTRLVDPRLAATMAEAVLAWTLYLHRDMPLYARR 127 Query: 183 QQARQWRPLDYVLPEQRRVGILGLGALGEAAAVALRQARFPVLGWSRRARNVPDVRTYSG 242 Q+ W YVLPEQRRV +LGLGALGEAAA ALRQARF V GWSR AR V+T+ G Sbjct: 128 QREHHWEAQPYVLPEQRRVSLLGLGALGEAAAAALRQARFAVNGWSRSAREAEGVQTFHG 187 Query: 243 MDGLAEMLAQTDILVCLLPLTAETRGLLDAARLALLPPQADLINFARGPIIDDEALRQAL 302 GLA+MLAQTDILVCLLPLTAETRGL+DAA LA+LPPQA LINFARGPIIDD AL+ AL Sbjct: 188 PAGLAQMLAQTDILVCLLPLTAETRGLIDAAHLAMLPPQAQLINFARGPIIDDAALKAAL 247 Query: 303 DAGKLRHAVLDVFATEPLPPEAWPWSHPAVTVLPHCSAPTDSGTASAIVAVNIRRYRNTG 362 DAG+LRHAVLDVFA EPLPP AWPWSHPAVTVLPHCSAPTD TASAIVA NIRRYR++G Sbjct: 248 DAGQLRHAVLDVFAVEPLPPAAWPWSHPAVTVLPHCSAPTDRQTASAIVAANIRRYRHSG 307 Query: 363 EIAANVDLKRGY 374 EIAANVDL+RGY Sbjct: 308 EIAANVDLRRGY 319 >ref|YP_001566649.1| NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase [Delftia acidovorans SPH-1] gb|ABX38264.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding [Delftia acidovorans SPH-1] Length = 323 Score = 359 bits (922), Expect = 3e-97, Method: Composition-based stats. Identities = 208/311 (66%), Positives = 235/311 (75%) Query: 64 PIPFVSQSDAAAVAAWLQALRAAMPEEEIIAFSELDEDQRAQCRVAIVANPDPAQLKLLP 123 P+PFV A AWLQAL+AA+PEE ++A LD DQRA C VAIVANP PA L+ LP Sbjct: 13 PLPFVCAPGYPAAQAWLQALQAALPEERVVALDTLDADQRAACEVAIVANPQPADLRTLP 72 Query: 124 QLQWVHSVWAGVERLVADLGQSDLQITRLVDPRLAATMAEAVLAWTLYLHRDMPHYARQQ 183 +LQWVHSVWAGVERLVADLG +DL+I RLVDP+LA TMAEAVLAWTLYLHRDMP YARQQ Sbjct: 73 RLQWVHSVWAGVERLVADLGDTDLRIVRLVDPQLADTMAEAVLAWTLYLHRDMPRYARQQ 132 Query: 184 QARQWRPLDYVLPEQRRVGILGLGALGEAAAVALRQARFPVLGWSRRARNVPDVRTYSGM 243 ARQW D V + R V +LGLGALG+AAA L A F V GWSR R++ V+ ++G Sbjct: 133 AARQWLAHDGVRAQDRTVSLLGLGALGKAAAQRLLSAGFQVRGWSRTPRHMDGVQCHAGD 192 Query: 244 DGLAEMLAQTDILVCLLPLTAETRGLLDAARLALLPPQADLINFARGPIIDDEALRQALD 303 L MLAQTDILVCLLPLT +TRGL+ A LA LP A LINFARGPI+DD ALR ALD Sbjct: 193 AELPAMLAQTDILVCLLPLTPQTRGLVGAQALAALPAHASLINFARGPIVDDAALRAALD 252 Query: 304 AGKLRHAVLDVFATEPLPPEAWPWSHPAVTVLPHCSAPTDSGTASAIVAVNIRRYRNTGE 363 G+L HAVLDVF EPLP +W W+HPAVTVLPH SAPTD TASAIVA NIRR+R +GE Sbjct: 253 GGRLAHAVLDVFEQEPLPEASWQWAHPAVTVLPHISAPTDRATASAIVAGNIRRWRASGE 312 Query: 364 IAANVDLKRGY 374 + VD RGY Sbjct: 313 LPQAVDAARGY 323 >ref|YP_004486349.1| hydroxypyruvate reductase [Delftia sp. Cs1-4] gb|AEF87994.1| Hydroxypyruvate reductase [Delftia sp. Cs1-4] Length = 320 Score = 358 bits (918), Expect = 1e-96, Method: Composition-based stats. Identities = 206/308 (66%), Positives = 234/308 (75%) Query: 67 FVSQSDAAAVAAWLQALRAAMPEEEIIAFSELDEDQRAQCRVAIVANPDPAQLKLLPQLQ 126 FV A AWLQAL+AA+PEE ++A LD DQRA C VAIVANP PA L+ LP+LQ Sbjct: 13 FVCAPGYPAAQAWLQALQAALPEERVVALDTLDADQRAACEVAIVANPQPADLRTLPRLQ 72 Query: 127 WVHSVWAGVERLVADLGQSDLQITRLVDPRLAATMAEAVLAWTLYLHRDMPHYARQQQAR 186 WVHSVWAGVERLVADLG +DL+I RLVDP+LAATMAEAVLAWTLYLHRDMP YARQQ AR Sbjct: 73 WVHSVWAGVERLVADLGDTDLRIVRLVDPQLAATMAEAVLAWTLYLHRDMPRYARQQAAR 132 Query: 187 QWRPLDYVLPEQRRVGILGLGALGEAAAVALRQARFPVLGWSRRARNVPDVRTYSGMDGL 246 QW D V + R V +LGLGALG+AAA L A F V GWSR R++ V+ ++G L Sbjct: 133 QWLAHDGVRAQDRTVSLLGLGALGKAAAQRLLSAGFQVRGWSRTPRHMESVQCHAGDAEL 192 Query: 247 AEMLAQTDILVCLLPLTAETRGLLDAARLALLPPQADLINFARGPIIDDEALRQALDAGK 306 MLAQTDILVCLLPLT +TRG++ A LA LP A LINFARGPI+DD ALR ALD+G+ Sbjct: 193 PAMLAQTDILVCLLPLTPQTRGIVGAQALAALPAHASLINFARGPIVDDAALRAALDSGR 252 Query: 307 LRHAVLDVFATEPLPPEAWPWSHPAVTVLPHCSAPTDSGTASAIVAVNIRRYRNTGEIAA 366 L HAVLDVF EPLP +W W+HPAVTVLPH SAPTD TASAIVA NIRR+R +GE+ Sbjct: 253 LAHAVLDVFEQEPLPEASWQWAHPAVTVLPHISAPTDRATASAIVAGNIRRWRASGELPQ 312 Query: 367 NVDLKRGY 374 VD RGY Sbjct: 313 AVDAARGY 320 >ref|ZP_08529222.1| 2-hydroxyacid dehydrogenase [Agrobacterium sp. ATCC 31749] gb|EGL64173.1| 2-hydroxyacid dehydrogenase [Agrobacterium sp. ATCC 31749] Length = 311 Score = 314 bits (804), Expect = 2e-83, Method: Composition-based stats. Identities = 177/310 (57%), Positives = 222/310 (71%) Query: 65 IPFVSQSDAAAVAAWLQALRAAMPEEEIIAFSELDEDQRAQCRVAIVANPDPAQLKLLPQ 124 I V++ A WL +L MP E I+ F +LDE R + +AIVANPDPA + LP Sbjct: 2 IVLVTRMSEDAERLWLTSLSRKMPGETIVPFRQLDEAAREKADIAIVANPDPADIARLPN 61 Query: 125 LQWVHSVWAGVERLVADLGQSDLQITRLVDPRLAATMAEAVLAWTLYLHRDMPHYARQQQ 184 L+W+HSVWAGVERLVA+LG+S + I RLVDP+L+ TMAEAVLAWT YL RDMP YA Q+ Sbjct: 62 LKWIHSVWAGVERLVAELGESRMPIVRLVDPQLSQTMAEAVLAWTYYLFRDMPAYANFQR 121 Query: 185 ARQWRPLDYVLPEQRRVGILGLGALGEAAAVALRQARFPVLGWSRRARNVPDVRTYSGMD 244 R W+ L Y P+ +G+LGLGALG +A LR A F V+GWSR + + V+T+ G Sbjct: 122 QRVWQQLAYRRPQDVTIGLLGLGALGAESAARLRHAGFNVIGWSRSHKEIDGVQTFHGDR 181 Query: 245 GLAEMLAQTDILVCLLPLTAETRGLLDAARLALLPPQADLINFARGPIIDDEALRQALDA 304 GL E+L +++ILVCLLPLT ETRGL+DA RL++LP A LINFARGPI+ + L +ALD+ Sbjct: 182 GLEELLGRSEILVCLLPLTGETRGLIDAVRLSMLPEGASLINFARGPIVVADDLIRALDS 241 Query: 305 GKLRHAVLDVFATEPLPPEAWPWSHPAVTVLPHCSAPTDSGTASAIVAVNIRRYRNTGEI 364 G ++HAVLDVF EPLPP+ W HPA+TVLPH SAPTD TA+AIVA NI YR++G + Sbjct: 242 GAMKHAVLDVFDVEPLPPDNKLWGHPAITVLPHISAPTDRETAAAIVAGNILTYRSSGAL 301 Query: 365 AANVDLKRGY 374 VDL RGY Sbjct: 302 PPTVDLARGY 311 >ref|NP_396409.2| 2-hydroxyacid dehydrogenase [Agrobacterium tumefaciens str. C58] gb|AAK90850.2| 2-hydroxyacid dehydrogenase [Agrobacterium tumefaciens str. C58] Length = 311 Score = 313 bits (803), Expect = 2e-83, Method: Composition-based stats. Identities = 179/310 (57%), Positives = 222/310 (71%) Query: 65 IPFVSQSDAAAVAAWLQALRAAMPEEEIIAFSELDEDQRAQCRVAIVANPDPAQLKLLPQ 124 I V++ A WL +L MP E I+ F +LDE R + +AIVANPDPA + LP Sbjct: 2 IVLVTRMSEDAERLWLTSLSRKMPGETIVPFRQLDEAAREKADIAIVANPDPADIARLPN 61 Query: 125 LQWVHSVWAGVERLVADLGQSDLQITRLVDPRLAATMAEAVLAWTLYLHRDMPHYARQQQ 184 L+W+HSVWAGVERLVA+LG+S L I RLVDP+L+ TMAEAVLAWT YL RDMP YA Q+ Sbjct: 62 LKWIHSVWAGVERLVAELGESRLPIVRLVDPQLSQTMAEAVLAWTYYLFRDMPAYANFQR 121 Query: 185 ARQWRPLDYVLPEQRRVGILGLGALGEAAAVALRQARFPVLGWSRRARNVPDVRTYSGMD 244 R W+ L Y P+ +G+LGLGALG +A LR A F V+GWSR + + V+T+ G Sbjct: 122 QRVWQQLAYRRPQDVTIGLLGLGALGAESAARLRYAGFNVIGWSRSHKEIDGVQTFHGDR 181 Query: 245 GLAEMLAQTDILVCLLPLTAETRGLLDAARLALLPPQADLINFARGPIIDDEALRQALDA 304 GL E+L +++ILVCLLPLT ETRGL+DA RL+LLP A LINFARGPI+ + L +ALD+ Sbjct: 182 GLEELLGRSEILVCLLPLTDETRGLIDAVRLSLLPEGASLINFARGPIVVADDLIRALDS 241 Query: 305 GKLRHAVLDVFATEPLPPEAWPWSHPAVTVLPHCSAPTDSGTASAIVAVNIRRYRNTGEI 364 G ++HAVLDVF EPLPP+ W HPA+TVLPH SAPTD TA+AIVA NI YR++G + Sbjct: 242 GAMKHAVLDVFDVEPLPPDNKLWDHPAITVLPHISAPTDRETAAAIVAGNILTYRSSGAL 301 Query: 365 AANVDLKRGY 374 VDL RGY Sbjct: 302 PPTVDLARGY 311