BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4848.p (416 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775896.1| iron-regulated membrane protein [Herbaspiril... 734 0.0 ref|YP_004753024.1| iron-uptake factor PiuB [Collimonas fungivor... 579 e-163 ref|YP_003527301.1| PepSY-associated TM helix domain-containing ... 514 e-143 ref|YP_003050193.1| PepSY-associated TM helix domain-containing ... 513 e-143 ref|YP_004038856.1| pepsy-associated tm helix domain-containing ... 513 e-143 >ref|YP_003775896.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] gb|ADJ63988.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 416 Score = 734 bits (1896), Expect = 0.0, Method: Composition-based stats. Identities = 363/414 (87%), Positives = 388/414 (93%) Query: 1 MRALLVLLHRWFGLAVAVFLFIAGVTGALISWDHELDAWLNPQLFHARSADRPDAGQPMP 60 MRA LVLLHRWFGLA AVFLFIAG+TGA+ISWDHELDAWLNPQLF ARSA +P AG PMP Sbjct: 1 MRATLVLLHRWFGLAAAVFLFIAGLTGAVISWDHELDAWLNPQLFDARSATQPGAGAPMP 60 Query: 61 GLELAQRVEAAEPKLRVTYVLTEAEPGHTISMMVEPRLDPATGKLPVLDYNQIAVDPVTG 120 GLELA+RVE AEP+LRVTYVLT+AEPGHTI MMVEPR+DPATGKL LDYNQIAVDP T Sbjct: 61 GLELARRVEQAEPRLRVTYVLTQAEPGHTIGMMVEPRVDPATGKLYTLDYNQIAVDPFTA 120 Query: 121 EIQGRRMWGAISLARENLLPFLYKLHYTMHLPDMGGIETGIWLMGIIGIVWALDCFIALY 180 EIQGRRMWGAISL+RENLLPFLYKLHYTMHLPD+GG+ETGI LMGIIGIVWALDC IALY Sbjct: 121 EIQGRRMWGAISLSRENLLPFLYKLHYTMHLPDLGGLETGILLMGIIGIVWALDCLIALY 180 Query: 181 LSFPNWRSWRKSLAFRWREGGHKLNFDLHRSGGVWIWGLLLVLAVTSVSMNLERQVMRPI 240 LSFPNWRSWRKSLAFRWR+GGHKLNFDLHRSGGVW+WGLLL+LAVTSVSMNLERQVMRP+ Sbjct: 181 LSFPNWRSWRKSLAFRWRDGGHKLNFDLHRSGGVWVWGLLLILAVTSVSMNLERQVMRPL 240 Query: 241 LQSVSTLTAGPFDTRTPIAPDKVPEPRISREEAVALGRAQGEKLGLTEPVGGVFYSGMFG 300 LQ++STLT PFDTRTP AP+KV EPRI+RE+AVALG+AQ K+GLTEPVGGVFYSGMFG Sbjct: 241 LQTLSTLTPSPFDTRTPTAPEKVAEPRITREQAVALGQAQARKMGLTEPVGGVFYSGMFG 300 Query: 301 VYGVGFFQPGNDHGDVGLGNAWLYFDAMSGAYEGGKIPGRGSAGDIFLQAQFPLHSGRIL 360 VYGVG+FQPGNDHGD+GLGNAWLYFDA SGAYEGGKIPGRGSAGDIFLQAQFPLHSGRIL Sbjct: 301 VYGVGYFQPGNDHGDIGLGNAWLYFDANSGAYEGGKIPGRGSAGDIFLQAQFPLHSGRIL 360 Query: 361 GLTGRVLISLMGVLVAMLSVTGVIIWVRKRRARLQQRERLAAQKTPAREGPVVE 414 GL GR+LIS MG LVAMLSVTGVIIWVRKR+ARLQQRER AAQKTPAREGPV E Sbjct: 361 GLPGRILISFMGALVAMLSVTGVIIWVRKRKARLQQRERTAAQKTPAREGPVAE 414 >ref|YP_004753024.1| iron-uptake factor PiuB [Collimonas fungivorans Ter331] gb|AEK62201.1| Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB [Collimonas fungivorans Ter331] Length = 421 Score = 579 bits (1493), Expect = e-163, Method: Composition-based stats. Identities = 300/418 (71%), Positives = 344/418 (82%), Gaps = 5/418 (1%) Query: 2 RALLVLLHRWFGLAVAVFLFIAGVTGALISWDHELDAWLNPQLFHARSADRPDAGQPMPG 61 R +V+LHRWFGLA AVFLFIAG TGA+ISWDHELDAWLNPQLFHA SAD+P +G+ +PG Sbjct: 4 RPFMVILHRWFGLAAAVFLFIAGATGAVISWDHELDAWLNPQLFHAYSADQPLSGKALPG 63 Query: 62 LELAQRVEAAEPKLRVTYVLTEAEPGHTISMMVEPRLDPATGKLPVLDYNQIAVDPVTGE 121 LELA RVEA +P+LRVTYV+TE+EPGHT SMMVE R+DPA+GK L +NQ+AVDPV+ E Sbjct: 64 LELANRVEAQDPRLRVTYVMTESEPGHTSSMMVEGRIDPASGKPYDLGFNQVAVDPVSAE 123 Query: 122 IQGRRMWGAISLARENLLPFLYKLHYTMHLPDMGGIETGIWLMGIIGIVWALDCFIALYL 181 IQG+RMWGAISL+RENLLPFLYKLHYTMHLP++GGIE G+WLMG+IGIVW LDCFIAL+L Sbjct: 124 IQGKRMWGAISLSRENLLPFLYKLHYTMHLPEVGGIELGVWLMGLIGIVWVLDCFIALWL 183 Query: 182 SFPNWRSWRKSLAFRWREGGHKLNFDLHRSGGVWIWGLLLVLAVTSVSMNLERQVMRPIL 241 SFPNWRSWRKS AFRWR+GGHK NFDLHRSGGVW+W LLL+LAVTSVSMNL VMRPI+ Sbjct: 184 SFPNWRSWRKSFAFRWRQGGHKFNFDLHRSGGVWLWMLLLLLAVTSVSMNLYNPVMRPIV 243 Query: 242 QSVSTLTAGPFDTRTPIAPDKVP--EPRISREEAVALGRAQGEKLGLTEPVGGVFYSGMF 299 Q+ STLT PFD+R P+AP EP +SRE L R G++ G PVGGVFYS F Sbjct: 244 QTFSTLTPDPFDSRKPVAPALAAKTEPAVSRERVAQLAREDGQRRGWMVPVGGVFYSSAF 303 Query: 300 GVYGVGFFQPGNDHGDVGLGNAWLYFDAMSGAYEGGKIPGRGSAGDIFLQAQFPLHSGRI 359 G+YGVGFF+ GNDHGDVGLGN+WLY+DA G G KIPG GSAGDIF+QAQFPLHSGRI Sbjct: 304 GLYGVGFFEAGNDHGDVGLGNSWLYYDAADGHAAGAKIPGSGSAGDIFMQAQFPLHSGRI 363 Query: 360 LGLTGRVLISLMGVLVAMLSVTGVIIWVRKRRARL---QQRERLAAQKTPAREGPVVE 414 LGLTGR+LISLMG +VA+LS TGVIIW RKRRAR+ + +LA K R V E Sbjct: 364 LGLTGRILISLMGAVVALLSATGVIIWARKRRARMLAARHAAQLALPKASRRRTAVAE 421 >ref|YP_003527301.1| PepSY-associated TM helix domain-containing protein [Nitrosococcus halophilus Nc4] gb|ADE14914.1| PepSY-associated TM helix domain protein [Nitrosococcus halophilus Nc4] Length = 416 Score = 514 bits (1323), Expect = e-143, Method: Composition-based stats. Identities = 260/412 (63%), Positives = 321/412 (77%), Gaps = 3/412 (0%) Query: 1 MRALLVLLHRWFGLAVAVFLFIAGVTGALISWDHELDAWLNPQLFHARSADRPDAGQPMP 60 MR LVLLHRW GL +AVFL IAG+TGA+ISWDHELD WLNPQLF ARS +AG+ + Sbjct: 1 MRLFLVLLHRWLGLFIAVFLLIAGLTGAVISWDHELDEWLNPQLFKARSGSE-EAGESLS 59 Query: 61 GLELAQRVEAAEPKLRVTYVLTEAEPGHTISMMVEPRLDPATGKLPVLDYNQIAVDPVTG 120 + LA R EAA+P++RVTY+ AEPGHT+ + V+PR+DPATG+ YNQ+A+DP+TG Sbjct: 60 PVALAARAEAADPRVRVTYLPLAAEPGHTVVLFVQPRIDPATGEAFEPGYNQVALDPMTG 119 Query: 121 EIQGRRMWGAISLARENLLPFLYKLHYTMHLPDMGGIETGIWLMGIIGIVWALDCFIALY 180 EIQGRR+WG I RENLLPFLYKLHY++H+PD G+E GI LMGI+GI+W LD FIAL+ Sbjct: 120 EIQGRRLWGEIGFNRENLLPFLYKLHYSLHIPDGWGLELGILLMGIVGIIWVLDSFIALW 179 Query: 181 LSFPNWRSWRKSLAFRWREGGHKLNFDLHRSGGVWIWGLLLVLAVTSVSMNLERQVMRPI 240 ++FP+ +WRKS AFRWR+GG+KLNFDLHRSGGVW+WGLL ++AVTSVSMNLE QVMRP+ Sbjct: 180 IAFPSAGAWRKSFAFRWRQGGYKLNFDLHRSGGVWVWGLLFIMAVTSVSMNLEFQVMRPV 239 Query: 241 LQSVSTLTAGPFDTRTPIAPDKVPEPRISREEAVALGRAQGEKLGLTEPVGGVFYSGMFG 300 + S L+ PF+TRT P++ EPRI R A+ + + + ++ G T P GG+FYS +G Sbjct: 240 VSVFSDLSPSPFETRTASPPNQPVEPRIDRARALEIAQTEADRRGWTVPAGGIFYSPAYG 299 Query: 301 VYGVGFFQPGNDHGDVGLGNAWLYFDAMSGAYEGGKIPGRGSAGDIFLQAQFPLHSGRIL 360 VYGVGFF PGNDHGD GLGN Y D M G+ G ++PG GS GDIF+QAQFPLHSGRIL Sbjct: 300 VYGVGFFTPGNDHGDGGLGNPRFYVDGMDGSLAGAEVPGSGSFGDIFMQAQFPLHSGRIL 359 Query: 361 GLTGRVLISLMGVLVAMLSVTGVIIWVRKRRARLQQRERLAAQKTPAREGPV 412 GL GR+LIS+MG++VAMLSVTG++IWVRKRRARL R Q PA GP+ Sbjct: 360 GLPGRILISVMGLVVAMLSVTGIVIWVRKRRARLVAGVR--KQNAPAATGPL 409 >ref|YP_003050193.1| PepSY-associated TM helix domain-containing protein [Methylovorus glucosetrophus SIP3-4] gb|ACT49666.1| PepSY-associated TM helix domain protein [Methylovorus glucosetrophus SIP3-4] Length = 419 Score = 513 bits (1322), Expect = e-143, Method: Composition-based stats. Identities = 252/409 (61%), Positives = 307/409 (75%), Gaps = 3/409 (0%) Query: 1 MRALLVLLHRWFGLAVAVFLFIAGVTGALISWDHELDAWLNPQLFHARSADRPDAGQPMP 60 MR L LLHRWFGLA A+FLFIAG+TGA+ISWDHELDA LNP LF + P G P P Sbjct: 1 MRKPLALLHRWFGLATAIFLFIAGLTGAVISWDHELDAALNPHLFELEA---PAHGIPQP 57 Query: 61 GLELAQRVEAAEPKLRVTYVLTEAEPGHTISMMVEPRLDPATGKLPVLDYNQIAVDPVTG 120 L+LA +EAA P ++VTY+ E GH + + V+PRL+PATGK D+NQ+AV+P TG Sbjct: 58 SLQLAAEIEAANPSIQVTYLPLATEAGHALQLFVQPRLNPATGKPYTPDFNQMAVNPYTG 117 Query: 121 EIQGRRMWGAISLARENLLPFLYKLHYTMHLPDMGGIETGIWLMGIIGIVWALDCFIALY 180 E+QG+RMWG ISLA ENLLPFLYKLHY++H+PD +E G+ MGII IVW +D IAL+ Sbjct: 118 EVQGKRMWGEISLAPENLLPFLYKLHYSLHIPDGFNLELGVLFMGIIAIVWTIDSLIALW 177 Query: 181 LSFPNWRSWRKSLAFRWREGGHKLNFDLHRSGGVWIWGLLLVLAVTSVSMNLERQVMRPI 240 +SFPN SWRKS AFRW++GG+ LNFDLHRSGGVW+WGLLL++AVTS+SMNL +VMRP+ Sbjct: 178 ISFPNLASWRKSFAFRWQKGGYALNFDLHRSGGVWVWGLLLIMAVTSISMNLGTEVMRPL 237 Query: 241 LQSVSTLTAGPFDTRTPIAPDKVPEPRISREEAVALGRAQGEKLGLTEPVGGVFYSGMFG 300 + +S LTA PFDT TP DK P ISR+ + ++ K G P G +F+S FG Sbjct: 238 VNRISPLTASPFDTITPSPLDKPLTPVISRQAVLDRATSEARKRGWEAPPGAIFWSPQFG 297 Query: 301 VYGVGFFQPGNDHGDVGLGNAWLYFDAMSGAYEGGKIPGRGSAGDIFLQAQFPLHSGRIL 360 VYGVGFF+ NDHGD GLGN WLYFD +G Y G +PG GSAGDIF+QAQFPLHSGRIL Sbjct: 298 VYGVGFFEADNDHGDGGLGNPWLYFDGKTGEYAGAIVPGTGSAGDIFMQAQFPLHSGRIL 357 Query: 361 GLTGRVLISLMGVLVAMLSVTGVIIWVRKRRARLQQRERLAAQKTPARE 409 GL GR+LISLMGV+V MLSVTG+IIW RKR++RL Q +R A+ R+ Sbjct: 358 GLPGRILISLMGVVVTMLSVTGIIIWARKRKSRLHQSQRAASTNVSKRQ 406 >ref|YP_004038856.1| pepsy-associated tm helix domain-containing protein [Methylovorus sp. MP688] gb|ADQ83620.1| PepSY-associated TM helix domain protein [Methylovorus sp. MP688] Length = 419 Score = 513 bits (1320), Expect = e-143, Method: Composition-based stats. Identities = 252/409 (61%), Positives = 308/409 (75%), Gaps = 3/409 (0%) Query: 1 MRALLVLLHRWFGLAVAVFLFIAGVTGALISWDHELDAWLNPQLFHARSADRPDAGQPMP 60 MR L LLHRWFGLA A+FLFIAG+TGA+ISWDHELDA LNP LF ++ P G P P Sbjct: 1 MRKPLALLHRWFGLATAIFLFIAGLTGAVISWDHELDAALNPHLFELQA---PAHGIPQP 57 Query: 61 GLELAQRVEAAEPKLRVTYVLTEAEPGHTISMMVEPRLDPATGKLPVLDYNQIAVDPVTG 120 L+LA +EAA P ++VTY+ E GH + + V+PRL+PATGK D+NQ+AV+P TG Sbjct: 58 SLQLAAEIEAANPSIQVTYLPLATEAGHALQLFVQPRLNPATGKPYTPDFNQMAVNPYTG 117 Query: 121 EIQGRRMWGAISLARENLLPFLYKLHYTMHLPDMGGIETGIWLMGIIGIVWALDCFIALY 180 E+QG+RMWG ISLA ENLLPFLYKLHY++H+PD +E G+ MGII IVW +D IAL+ Sbjct: 118 EVQGKRMWGEISLAPENLLPFLYKLHYSLHIPDGFNLELGVLFMGIIAIVWTIDSLIALW 177 Query: 181 LSFPNWRSWRKSLAFRWREGGHKLNFDLHRSGGVWIWGLLLVLAVTSVSMNLERQVMRPI 240 +SFPN SWRKS AFRW++GG+ LNFDLHRSGGVW+WGLLL++AVTS+SMNL +VMRP+ Sbjct: 178 ISFPNLASWRKSFAFRWQKGGYALNFDLHRSGGVWVWGLLLIMAVTSISMNLGTEVMRPL 237 Query: 241 LQSVSTLTAGPFDTRTPIAPDKVPEPRISREEAVALGRAQGEKLGLTEPVGGVFYSGMFG 300 + +S LTA PFDT TP DK P ISR+ + ++ K G P G +F+S FG Sbjct: 238 VNRISPLTASPFDTITPSPLDKPLTPVISRQAVLDRATSEARKRGWEAPPGAIFWSPQFG 297 Query: 301 VYGVGFFQPGNDHGDVGLGNAWLYFDAMSGAYEGGKIPGRGSAGDIFLQAQFPLHSGRIL 360 VYGVGFF+ NDHGD GLGN WLYFD +G Y G +PG GSAGDIF+QAQFPLHSGRIL Sbjct: 298 VYGVGFFEADNDHGDGGLGNPWLYFDGKTGEYAGAIVPGTGSAGDIFMQAQFPLHSGRIL 357 Query: 361 GLTGRVLISLMGVLVAMLSVTGVIIWVRKRRARLQQRERLAAQKTPARE 409 GL GR+LISLMGV+V MLSVTG+IIW RKR++RL Q +R A+ R+ Sbjct: 358 GLPGRILISLMGVVVTMLSVTGIIIWARKRKSRLHQSQRAASTDVSKRQ 406