BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4822.p (332 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775918.1| D-lactate dehydrogenase [Herbaspirillum sero... 546 e-153 ref|YP_001637534.1| NAD-binding D-isomer specific 2-hydroxyacid ... 378 e-103 ref|YP_002005574.1| fermentative d-lactate dehydrogenase, NAD-de... 377 e-102 ref|YP_001866017.1| D-isomer specific 2-hydroxyacid dehydrogenas... 375 e-102 ref|YP_002420091.1| NAD-binding D-isomer specific 2-hydroxyacid ... 374 e-101 >ref|YP_003775918.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] gb|ADJ64010.1| D-lactate dehydrogenase protein [Herbaspirillum seropedicae SmR1] Length = 330 Score = 546 bits (1407), Expect = e-153, Method: Composition-based stats. Identities = 297/332 (89%), Positives = 308/332 (92%), Gaps = 2/332 (0%) Query: 1 MKTAVFSARRYDQTLLTRANAASGAAHELVFLQDRLDLDTAQLASGCQAVAAFVNDDLSA 60 MKTAVFSARRYDQTLL RANAA G HELVFLQDRL+ TAQLA+GCQAVAAFVNDDL Sbjct: 1 MKTAVFSARRYDQTLLARANAAHG--HELVFLQDRLEAGTAQLAAGCQAVAAFVNDDLGG 58 Query: 61 AVLERLAMLGVRFVTTRSTGFNHIDTQAARRLGITVARVADYSPYSVAEFAVGLLLAVNR 120 VLE+LA LGVRFVTTRSTGFNHID AAR LG+TVARVADYSPYSVAEFAVGLL AVNR Sbjct: 59 VVLEKLAELGVRFVTTRSTGFNHIDVAAARALGLTVARVADYSPYSVAEFAVGLLQAVNR 118 Query: 121 KIARASMRTREGNFELEGLMGFDLHGKTVGVIGTGKIGALFARIMAGFGCIVLGSDVHHN 180 KIARASMRTREGNFEL+GLMG DLHGKTVGVIGTGKIG LFARIMAGFGC +LGSDVH N Sbjct: 119 KIARASMRTREGNFELDGLMGVDLHGKTVGVIGTGKIGVLFARIMAGFGCQILGSDVHRN 178 Query: 181 PDFEALGGRYVRREELFAASDVISLHCPLTEATRYLVNAETLALAKPGCILVNTSRGGLV 240 PDF+ALGGRYV R ELFAASDVISLHCPLTEATRYLVNA++LALAKPGCILVNTSRGGLV Sbjct: 179 PDFDALGGRYVERTELFAASDVISLHCPLTEATRYLVNAQSLALAKPGCILVNTSRGGLV 238 Query: 241 DTEAAIAALKSGQLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAF 300 DTEAAIAALKSG+LRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAF Sbjct: 239 DTEAAIAALKSGRLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAF 298 Query: 301 FTEEAIGQIMQTTIANLNDFAAGKQLGERLVA 332 FTEEAIGQIMQTTI NL+ FAAG LGERLVA Sbjct: 299 FTEEAIGQIMQTTIENLSAFAAGDDLGERLVA 330 >ref|YP_001637534.1| NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase [Methylobacterium extorquens PA1] gb|ABY28463.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding [Methylobacterium extorquens PA1] Length = 335 Score = 378 bits (971), Expect = e-103, Method: Composition-based stats. Identities = 202/332 (60%), Positives = 249/332 (75%), Gaps = 3/332 (0%) Query: 1 MKTAVFSARRYDQTLLTRANAASGAAHELVFLQDRLDLDTAQLASGCQAVAAFVNDDLSA 60 M+ AVFSA+ Y++ LL NA G H+L + L+ TA LA+G AV+ FVND ++ Sbjct: 1 MRVAVFSAKNYERALLDDLNA--GQEHDLTYFDALLEPGTASLAAGFTAVSVFVNDSVNR 58 Query: 61 AVLERLAMLGVRFVTTRSTGFNHIDTQAARRLGITVARVADYSPYSVAEFAVGLLLAVNR 120 VL +LA G + V TR TGFNHID +AA GI V RV +YSP SVAEFAVGLLLA+NR Sbjct: 59 DVLRQLAQGGTKLVATRCTGFNHIDLEAAEEFGIRVVRVVNYSPNSVAEFAVGLLLALNR 118 Query: 121 KIARASMRTREGNFELEGLMGFDLHGKTVGVIGTGKIGALFARIMAGFGCIVLGSDVHHN 180 K+ RA RTREGNF L+GLMGFDL +TV VIGTGKIG +F RIMAGFGC V+G DVH + Sbjct: 119 KVHRAYNRTREGNFRLDGLMGFDLVDRTVAVIGTGKIGTIFGRIMAGFGCRVIGFDVHRS 178 Query: 181 PDFEALGGRYVRREELFAASDVISLHCPLTEATRYLVNAETLALAKPGCILVNTSRGGLV 240 P+FE +GGRYV + A DVIS+HCPLT T ++V+A TL K G +L+NTSRGGL+ Sbjct: 179 PEFERIGGRYVEAAGVEEA-DVISMHCPLTPETYHIVDARTLGRVKKGALLINTSRGGLI 237 Query: 241 DTEAAIAALKSGQLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAF 300 DT+AAI ALKSG+L GLAIDVYEQEA LF++DLSST+I DDVIQRL+SFPNVIVTGHQAF Sbjct: 238 DTDAAIDALKSGRLGGLAIDVYEQEAELFYRDLSSTVIPDDVIQRLISFPNVIVTGHQAF 297 Query: 301 FTEEAIGQIMQTTIANLNDFAAGKQLGERLVA 332 FT+EA+G I+ TT+ ++++ AG L + A Sbjct: 298 FTQEALGTILGTTLKSISELEAGHPLTNEVAA 329 >ref|YP_002005574.1| fermentative d-lactate dehydrogenase, NAD-dependent [Cupriavidus taiwanensis LMG 19424] emb|CAQ69507.1| fermentative D-lactate dehydrogenase, NAD-dependent [Cupriavidus taiwanensis LMG 19424] Length = 331 Score = 377 bits (969), Expect = e-102, Method: Composition-based stats. Identities = 203/332 (61%), Positives = 246/332 (74%), Gaps = 2/332 (0%) Query: 1 MKTAVFSARRYDQTLLTRANAASGAAHELVFLQDRLDLDTAQLASGCQAVAAFVNDDLSA 60 M+ AVFSA+ YD+ L ANA G H+L + + LD +T LA+G AV FVND A Sbjct: 1 MEIAVFSAKSYDRQHLDAANAVEG--HQLKYFEVPLDTETVGLAAGHGAVCIFVNDRADA 58 Query: 61 AVLERLAMLGVRFVTTRSTGFNHIDTQAARRLGITVARVADYSPYSVAEFAVGLLLAVNR 120 VLE L G R V R TGFN++D +AA+ LG+ V RV DYSP SVAE AV LL+AVNR Sbjct: 59 TVLEALGRGGTRLVALRCTGFNNVDLKAAQALGVKVVRVVDYSPNSVAEHAVALLMAVNR 118 Query: 121 KIARASMRTREGNFELEGLMGFDLHGKTVGVIGTGKIGALFARIMAGFGCIVLGSDVHHN 180 KI RA RTR+ NF LEGLMGFDL GKTV VIGTGKIG +FA+IM GFGC V+G D + + Sbjct: 119 KIHRAYNRTRDFNFSLEGLMGFDLCGKTVAVIGTGKIGRVFAKIMVGFGCNVIGYDKYPS 178 Query: 181 PDFEALGGRYVRREELFAASDVISLHCPLTEATRYLVNAETLALAKPGCILVNTSRGGLV 240 P+FEALGGRY E+ A++D ISLHCPLT T +++NAETL+ AKPG +L+NTSRGGL+ Sbjct: 179 PEFEALGGRYADEGEIGASADCISLHCPLTPETHHIINAETLSRAKPGALLINTSRGGLI 238 Query: 241 DTEAAIAALKSGQLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAF 300 DTEA IAAL+SGQL GLAIDVYEQEA LFF+DLS TII D V+Q+LV+FPNVIVTGHQAF Sbjct: 239 DTEAVIAALRSGQLGGLAIDVYEQEAGLFFRDLSGTIIDDSVLQQLVTFPNVIVTGHQAF 298 Query: 301 FTEEAIGQIMQTTIANLNDFAAGKQLGERLVA 332 T EA+ I +TT+ ++ +F GK L + A Sbjct: 299 LTREAVMTICETTLRSVTEFERGKSLTNEVSA 330 >ref|YP_001866017.1| D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding [Nostoc punctiforme PCC 73102] gb|ACC81074.1| D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding [Nostoc punctiforme PCC 73102] Length = 334 Score = 375 bits (962), Expect = e-102, Method: Composition-based stats. Identities = 187/332 (56%), Positives = 237/332 (71%), Gaps = 2/332 (0%) Query: 1 MKTAVFSARRYDQTLLTRANAASGAAHELVFLQDRLDLDTAQLASGCQAVAAFVNDDLSA 60 MK AVFS + YD+ L+ N+ + HELVF + RL+ DTA LA+G AV FV+D + A Sbjct: 1 MKVAVFSTKVYDRQFLSTVNSPT--QHELVFFEPRLNRDTAILAAGFPAVCVFVHDQVDA 58 Query: 61 AVLERLAMLGVRFVTTRSTGFNHIDTQAARRLGITVARVADYSPYSVAEFAVGLLLAVNR 120 L+ LA G R V R GFN++D QAA LGITV RV YSPY VAE AVGL+L++NR Sbjct: 59 PTLKLLASRGTRLVVLRCAGFNNVDLQAAADLGITVVRVPAYSPYGVAEHAVGLILSLNR 118 Query: 121 KIARASMRTREGNFELEGLMGFDLHGKTVGVIGTGKIGALFARIMAGFGCIVLGSDVHHN 180 KI RA R REGNF L+GL+GF+LH +TVG++GTGKIG + +IM GFGC +L DV+ N Sbjct: 119 KIHRAYNRVREGNFSLDGLLGFNLHERTVGIVGTGKIGLILGQIMKGFGCNLLAYDVYRN 178 Query: 181 PDFEALGGRYVRREELFAASDVISLHCPLTEATRYLVNAETLALAKPGCILVNTSRGGLV 240 P+ EALGG+YV ELFA SD+ISLHCPLT T +L+NAE + KPG +L+NTSRG L+ Sbjct: 179 PELEALGGKYVELPELFANSDIISLHCPLTPETHHLINAEAIEQIKPGVMLINTSRGALI 238 Query: 241 DTEAAIAALKSGQLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAF 300 DT+A I LKSG++ L +DVYEQE+ LFF+DLS II DD+ QRL +FPNV++TGHQAF Sbjct: 239 DTQAVIEGLKSGKIGYLGVDVYEQESELFFEDLSGEIIQDDIFQRLTTFPNVLITGHQAF 298 Query: 301 FTEEAIGQIMQTTIANLNDFAAGKQLGERLVA 332 FT EA+ I +TT AN+ D G+ + A Sbjct: 299 FTAEALHNIAETTFANIADVENGRPCANEIRA 330 >ref|YP_002420091.1| NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase [Methylobacterium chloromethanicum CM4] gb|ACK82163.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding [Methylobacterium chloromethanicum CM4] Length = 335 Score = 374 bits (961), Expect = e-101, Method: Composition-based stats. Identities = 200/332 (60%), Positives = 247/332 (74%), Gaps = 3/332 (0%) Query: 1 MKTAVFSARRYDQTLLTRANAASGAAHELVFLQDRLDLDTAQLASGCQAVAAFVNDDLSA 60 M+ AVFSA+ Y++ LL N G H+L + L+ TA LA+G AV+ FVND ++ Sbjct: 1 MRVAVFSAKNYERALLDDLNV--GQEHDLTYFDALLEPGTASLAAGFTAVSVFVNDSVNR 58 Query: 61 AVLERLAMLGVRFVTTRSTGFNHIDTQAARRLGITVARVADYSPYSVAEFAVGLLLAVNR 120 VL +LA G + V TR TGFNHID +AA GI V RV +YSP SVAEFAVGLLLA+NR Sbjct: 59 DVLRQLAQGGTKLVATRCTGFNHIDLEAAEEFGIRVVRVVNYSPNSVAEFAVGLLLALNR 118 Query: 121 KIARASMRTREGNFELEGLMGFDLHGKTVGVIGTGKIGALFARIMAGFGCIVLGSDVHHN 180 K+ RA RTREGNF L+GLMGFDL +TV VIGTGKIG +F RIMAGFGC V+G DVH + Sbjct: 119 KVHRAYNRTREGNFRLDGLMGFDLVDRTVAVIGTGKIGTIFGRIMAGFGCRVIGFDVHRS 178 Query: 181 PDFEALGGRYVRREELFAASDVISLHCPLTEATRYLVNAETLALAKPGCILVNTSRGGLV 240 P+FE +GGRYV + A DVIS+HCPLT T ++V+A TL K G +L+NTSRGGL+ Sbjct: 179 PEFERIGGRYVDAAGVEEA-DVISMHCPLTPETYHIVDARTLGRVKKGALLINTSRGGLI 237 Query: 241 DTEAAIAALKSGQLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAF 300 DT+AAI ALKSG+L GLAIDVYEQEA LF++DLSST+I DDVIQRL+SFPNVIVTGHQAF Sbjct: 238 DTDAAIDALKSGRLGGLAIDVYEQEAELFYRDLSSTVIPDDVIQRLISFPNVIVTGHQAF 297 Query: 301 FTEEAIGQIMQTTIANLNDFAAGKQLGERLVA 332 FT+EA+ I+ TT+ ++++ AG L + A Sbjct: 298 FTQEALETILGTTLKSISELEAGHPLTNEVAA 329