BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4785.p (342 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775947.1| sphingosine kinase/eukaryotic diacylglycerol... 526 e-147 ref|YP_001619945.1| hypothetical protein sce9292 [Sorangium cell... 228 2e-57 ref|NP_109525.1| hypothetical protein mll9677 [Mesorhizobium lot... 192 7e-47 ref|NP_108258.1| hypothetical protein mll8080 [Mesorhizobium lot... 184 3e-44 ref|ZP_06898350.1| diacylglycerol kinase catalytic region protei... 179 5e-43 >ref|YP_003775947.1| sphingosine kinase/eukaryotic diacylglycerol kinase [Herbaspirillum seropedicae SmR1] gb|ADJ64039.1| sphingosine kinase/eukaryotic diacylglycerol kinase protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 526 bits (1356), Expect = e-147, Method: Composition-based stats. Identities = 277/344 (80%), Positives = 297/344 (86%), Gaps = 7/344 (2%) Query: 1 MTEQDTTQELPASHGHPDAARRPDVVAAINARAGGGHAEELAAHITEEFARHGLHAVVHL 60 M +Q+TT LP P + RPDVVA INA+AGGGHAEELAA I+ EFARHG HA V L Sbjct: 1 MPDQNTTSPLP-----PHSQARPDVVAVINAKAGGGHAEELAARISAEFARHGRHARVRL 55 Query: 61 ARSGEDMLQAARNAVRDKIAVVAVGGGDGSVNAVASTLLADPHCESALGVLPLGTLNHFA 120 A SGEDML AR AVRD++ VVAVGGGDGSVNAVASTLLAD C+SALGVLPLGTLNHFA Sbjct: 56 AASGEDMLATARQAVRDRVPVVAVGGGDGSVNAVASTLLADAQCQSALGVLPLGTLNHFA 115 Query: 121 KDLNIPLTLDEAIANIATGRKLRVDSGEVNGAPFINNSSLGLYPDIVREREKQQARLGRG 180 KDLNIPLTL+ AIANIATGR+LRVDS EVNG FINNSSLGLYPDIVREREKQQARLGRG Sbjct: 116 KDLNIPLTLEGAIANIATGRRLRVDSAEVNGVAFINNSSLGLYPDIVREREKQQARLGRG 175 Query: 181 KWLAFTWAAMGALRRYPFLRVRLSIDGKEHWRRTPFVFIGNNEYLMSGLDIGKRSTLTDG 240 KWLAFTWAAMGALRRYPFLRVRLSIDG+EHWRRTPFVFIGNN YLMSGLDIGKRS+LT+G Sbjct: 176 KWLAFTWAAMGALRRYPFLRVRLSIDGQEHWRRTPFVFIGNNPYLMSGLDIGKRSSLTEG 235 Query: 241 QLSLYVCHRTGRLGLLRLALHALFGRLREAHDFDVLTCSEIHIETHKKRMRVATDGEVNL 300 LSLYVCH TGR GLLRLALHALFGRLR+AHDFD+LT ++IHIETHK+RMRVATDGEV L Sbjct: 236 CLSLYVCHHTGRWGLLRLALHALFGRLRQAHDFDMLTATDIHIETHKRRMRVATDGEVQL 295 Query: 301 MQTPLHYRIRPASLTVIVPQVADPQAPSTSEP--GLLDKLLGEN 342 MQTPL YRIRPASL VIVPQ A + S P GLLDKL GE+ Sbjct: 296 MQTPLSYRIRPASLEVIVPQAPASAATTASPPTGGLLDKLWGES 339 >ref|YP_001619945.1| hypothetical protein sce9292 [Sorangium cellulosum 'So ce 56'] emb|CAN99465.1| hypothetical protein sce9292 [Sorangium cellulosum 'So ce 56'] Length = 374 Score = 228 bits (580), Expect = 2e-57, Method: Composition-based stats. Identities = 123/309 (39%), Positives = 180/309 (58%), Gaps = 3/309 (0%) Query: 11 PASHGHPDAARRPDVVAAINARAGGGHAEELAAHITEEFARHGLHAVVHLARSGEDMLQA 70 P H + + +N + A A + E GL A V G+ + + Sbjct: 45 PLPHAGRATVKHRSMAVLLNCSSRSNRAAAQRARLGELLKESGLAAEVLCTEKGQQLGEL 104 Query: 71 ARNAVRDKIAVVAVGGGDGSVNAVASTLLADPHCESALGVLPLGTLNHFAKDLNIPLTLD 130 AR R +V GGGDG+VN VA+ + + +GVLPLGT+NHFAK L +PL L+ Sbjct: 105 ARRVARGPCDLVVAGGGDGTVNTVAAAIAG---TDKTMGVLPLGTVNHFAKHLGVPLALE 161 Query: 131 EAIANIATGRKLRVDSGEVNGAPFINNSSLGLYPDIVREREKQQARLGRGKWLAFTWAAM 190 A+ + GR++RVD GEVNG F+NNS+LG+YP IVR RE + R G KW+A + + Sbjct: 162 GAVRTLVEGRRVRVDVGEVNGRVFVNNSTLGIYPGIVRAREALRKRRGLNKWIALALSTV 221 Query: 191 GALRRYPFLRVRLSIDGKEHWRRTPFVFIGNNEYLMSGLDIGKRSTLTDGQLSLYVCHRT 250 +RR+ +R+RL +DG+E TPFVF+ NN++ + +DIG++ G+L + V H Sbjct: 222 SGIRRHERVRLRLVLDGQEVELETPFVFVANNDFKLGDMDIGRQRPPCSGELGVCVAHAE 281 Query: 251 GRLGLLRLALHALFGRLREAHDFDVLTCSEIHIETHKKRMRVATDGEVNLMQTPLHYRIR 310 RL L+L A+ GR+ +++DF +L +E+ +E +R+ VA DGEV M PL YR+R Sbjct: 282 SRLSTLKLIASAVVGRIHQSNDFSILRTTELRVEAPHRRLHVAADGEVLRMAPPLCYRVR 341 Query: 311 PASLTVIVP 319 PA+L VIVP Sbjct: 342 PAALRVIVP 350 >ref|NP_109525.1| hypothetical protein mll9677 [Mesorhizobium loti MAFF303099] dbj|BAB54864.1| mll9677 [Mesorhizobium loti MAFF303099] Length = 480 Score = 192 bits (488), Expect = 7e-47, Method: Composition-based stats. Identities = 120/313 (38%), Positives = 174/313 (55%), Gaps = 12/313 (3%) Query: 19 AARRPDVVAAINARAGGGHAE-ELAAHITEEFARHGLHAVVHLARSGEDMLQAARNAVRD 77 A+ V+A IN RAG E ++ + + FA G+ A V L E A R + Sbjct: 163 GAKDLKVMALINKRAGAAAREGDIERAVRDGFAERGVEADVRLVEGREVGELAQRFVAEN 222 Query: 78 KIA-----VVAVGGGDGSVNAVASTLLADPHCESALGVLPLGTLNHFAKDLNIPLTLDEA 132 K + ++ VGGGDG++ + AS L + LGVLPLGTLNHFAKDL +PL L A Sbjct: 223 KSSSGHGSILVVGGGDGTLGSAASALAG---TDVVLGVLPLGTLNHFAKDLGVPLDLTAA 279 Query: 133 IANIATGRKLRVDSGEVNGAPFINNSSLGLYPDIVREREKQQARLGRGKWLAFTWAAMGA 192 I IATG+ + VD EVNG F+NNSS+G+YP V +R +Q R G GK A A M Sbjct: 280 IDTIATGKPVAVDVAEVNGRVFLNNSSIGIYPFFVAKRSAEQRRRGLGKLAAIGPALMRT 339 Query: 193 LRRYPFLRVRLSIDGKEHWRRTPFVFIGNNEYLMSGLDIGKRSTLTDGQLSLYVCHRTGR 252 LR + V ++ G RTP VF+GNN Y ++ D+G+R +L+ +L +YV + Sbjct: 340 LRFASWQAVHVAAQGTRERLRTPCVFVGNNFYDIA--DLGRRRSLSSKELCVYVVKQQSW 397 Query: 253 LGLLRLALHALFGRLREAHDFDVLTCSEIHIETHKKRMRVATDGEVNLMQTPLHYRIRPA 312 GL L FG + D ++ + + I +H++ M ++ DGE + TPL+++IRPA Sbjct: 398 FGLALLPFKIAFGMVDATRDLEIYRTNSLQITSHRRAMLISLDGEAVSINTPLNFQIRPA 457 Query: 313 SLTVIVP-QVADP 324 +L V P ++ +P Sbjct: 458 ALQVFAPAKIPEP 470 >ref|NP_108258.1| hypothetical protein mll8080 [Mesorhizobium loti MAFF303099] dbj|BAB53719.1| mll8080 [Mesorhizobium loti MAFF303099] Length = 285 Score = 184 bits (466), Expect = 3e-44, Method: Composition-based stats. Identities = 111/276 (40%), Positives = 154/276 (55%), Gaps = 10/276 (3%) Query: 49 FARHGLHAVVHLARSGEDMLQAARNAVRDKIA-----VVAVGGGDGSVNAVASTLLADPH 103 FA G+ A V L E A R +K + ++ VGGGDG++ + AS L Sbjct: 5 FAECGVEADVRLVEGREVGELAQRLVSENKSSSGHGSILVVGGGDGTLGSAASALAG--- 61 Query: 104 CESALGVLPLGTLNHFAKDLNIPLTLDEAIANIATGRKLRVDSGEVNGAPFINNSSLGLY 163 + LGVLPLGTLNHFAKDL +PL L AI IATG+ + VD EVNG F+NNSS+G+Y Sbjct: 62 TDVVLGVLPLGTLNHFAKDLGVPLDLTAAIDTIATGKPVAVDVAEVNGRVFLNNSSIGIY 121 Query: 164 PDIVREREKQQARLGRGKWLAFTWAAMGALRRYPFLRVRLSIDGKEHWRRTPFVFIGNNE 223 P V +R +Q R G GK A A M LR + V +S G RTP VF+GNN Sbjct: 122 PFFVAKRSAEQRRRGFGKLAAIGPALMRTLRFASWQAVHVSAQGARERLRTPCVFVGNNF 181 Query: 224 YLMSGLDIGKRSTLTDGQLSLYVCHRTGRLGLLRLALHALFGRLREAHDFDVLTCSEIHI 283 Y ++ D+G+R +L +L +YV + GL L FG + D ++ + + I Sbjct: 182 YDIA--DLGRRRSLPSKELCVYVVKQQSWFGLALLPFKIAFGMVDATRDLEIYRTNSLQI 239 Query: 284 ETHKKRMRVATDGEVNLMQTPLHYRIRPASLTVIVP 319 +H++ M ++ DGE + TPL+++IRPA+L V P Sbjct: 240 TSHRRAMLISLDGEAVSINTPLNFQIRPATLQVFAP 275 >ref|ZP_06898350.1| diacylglycerol kinase catalytic region protein [Roseomonas cervicalis ATCC 49957] gb|EFH09961.1| diacylglycerol kinase catalytic region protein [Roseomonas cervicalis ATCC 49957] Length = 298 Score = 179 bits (454), Expect = 5e-43, Method: Composition-based stats. Identities = 126/291 (43%), Positives = 168/291 (57%), Gaps = 7/291 (2%) Query: 29 INARAGGGHAEELAAHITEEFARHGLHAVVHLARSGEDMLQAARNAVRDKIAVVAVGGGD 88 +NA G + L I FA G+ A V L GE++ +AA+ D VVA GG D Sbjct: 7 LNAAGGSAGDDTLPDRIASAFAEAGVEARV-LTPPGEELAEAAQTPGYD--GVVACGG-D 62 Query: 89 GSVNAVASTLLADPHCESALGVLPLGTLNHFAKDLNIPLTLDEAIANIATGRKLRVDSGE 148 G++NAVA+ L+ + LGVLPLGTLNHFA+DL +P+ +EA+ IA G+ R+D GE Sbjct: 63 GTINAVANALVGG---DVPLGVLPLGTLNHFARDLGLPVAWEEAVGVIAAGQTRRIDVGE 119 Query: 149 VNGAPFINNSSLGLYPDIVREREKQQARLGRGKWLAFTWAAMGALRRYPFLRVRLSIDGK 208 VNG F+NNSS+GLY ++V ER++Q+ R G K A A LRR+ R+ + +G Sbjct: 120 VNGRIFVNNSSVGLYAEMVTERDRQRRRRGWRKGPAMLLAGWRTLRRFTRRRLVIRAEGA 179 Query: 209 EHWRRTPFVFIGNNEYLMSGLDIGKRSTLTDGQLSLYVCHRTGRLGLLRLALHALFGRLR 268 R+P VFIGNN Y S + G R L G+L L V R GLLR L A+ G+LR Sbjct: 180 RAVLRSPLVFIGNNLYETSLPNPGTRHALDKGELCLAVTRHQSRWGLLRSGLRAMMGQLR 239 Query: 269 EAHDFDVLTCSEIHIETHKKRMRVATDGEVNLMQTPLHYRIRPASLTVIVP 319 E DF + S + I++ +RV+ DGEV M PL YR RP +L V P Sbjct: 240 EDRDFTLTRVSAVDIDSPLPMLRVSYDGEVRSMAPPLRYRTRPGALLVFAP 290