BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4758.p (369 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775965.1| poly(3-hydroxybutyrate) depolymerase [Herbas... 506 e-141 ref|YP_004271386.1| EF hand repeat-containing protein [Planctomy... 180 3e-43 ref|YP_003371725.1| phospholipase/carboxylesterase [Pirellula st... 168 1e-39 ref|ZP_05843057.1| polyhydroxybutyrate depolymerase [Rhodobacter... 150 4e-34 ref|YP_004316121.1| phospholipase/carboxylesterase [Sphingobacte... 148 1e-33 >ref|YP_003775965.1| poly(3-hydroxybutyrate) depolymerase [Herbaspirillum seropedicae SmR1] gb|ADJ64057.1| poly(3-hydroxybutyrate) depolymerase protein [Herbaspirillum seropedicae SmR1] Length = 369 Score = 506 bits (1304), Expect = e-141, Method: Composition-based stats. Identities = 274/339 (80%), Positives = 297/339 (87%), Gaps = 8/339 (2%) Query: 1 MTNRFPSR----PPDRA--RRLRRHLLAALCALPLLANLP--AQAIDLSLKTIRVGNLSR 52 MT R SR PP R+ R RR L+A L L L P AQA+DLSLKTIRVGNLSR Sbjct: 1 MTIRLLSRLRPFPPLRSLPRLSRRGLMACLLGLGLGVAAPPSAQAVDLSLKTIRVGNLSR 60 Query: 53 AYFSERNAVGEPQPLIVALHASGSSGSLMARATGLTEIAEAAGYMIVYPNGTGLAIDART 112 AYFSER +VGE +PLI+ALHASGSSGSLMARATGLTEIAEAAGYMI YPNGTGLAIDART Sbjct: 61 AYFSERTSVGEARPLIIALHASGSSGSLMARATGLTEIAEAAGYMIAYPNGTGLAIDART 120 Query: 113 WNSGGCCGYAQMHKVDDVAFLRALIDKLVKDGLADPQRIYLVGVSNGGMMAYRMAAEAPE 172 WNSGGCCGYAQMHKVDDVAFLRAL++KL KDGLADPQR+YLVGVSNGGMMAYRMAAEAP Sbjct: 121 WNSGGCCGYAQMHKVDDVAFLRALVEKLAKDGLADPQRVYLVGVSNGGMMAYRMAAEAPG 180 Query: 173 LFKAVAVVSAVLDVPPEAVKAPMPLLHIHGSDDPFIPFLGGIGKQEVSQLPRISVARSIE 232 LFKAVAVVSAVLDVPPE VKAPMP+LHIHGS+DPFIPFLGGIGKQEVSQLPR+SVAR+IE Sbjct: 181 LFKAVAVVSAVLDVPPETVKAPMPVLHIHGSEDPFIPFLGGIGKQEVSQLPRLSVARTIE 240 Query: 233 AFVKADGANPRPTVTDIPDTANDGTTIRQYTYASKSDPQAVVLYEVKGGGHAWPGGVVPI 292 +VKA+GA PRP VTDI DTANDGTTIRQYTY SK+DPQ+VVLYEVKGGGHAWPG V+PI Sbjct: 241 TWVKANGAEPRPAVTDIADTANDGTTIRQYTYRSKTDPQSVVLYEVKGGGHAWPGAVIPI 300 Query: 293 VNGGKSSQNLDTSRNVINFFNAHGGGRKSEPEDDDLPPG 331 +NGGKSSQN+D SR V+ FFNAHGGGR E D++LPPG Sbjct: 301 INGGKSSQNMDASRTVVEFFNAHGGGRSDEAVDENLPPG 339 >ref|YP_004271386.1| EF hand repeat-containing protein [Planctomyces brasiliensis DSM 5305] gb|ADY61364.1| EF hand repeat-containing protein [Planctomyces brasiliensis DSM 5305] Length = 391 Score = 180 bits (457), Expect = 3e-43, Method: Composition-based stats. Identities = 109/293 (37%), Positives = 161/293 (54%), Gaps = 18/293 (6%) Query: 37 AIDLSLKTIRVGNLSRAY---FSERNAVGEPQPLIVALHASGSSGSLMARATGLTEIAEA 93 AI SL+ ++VG+L R Y + + P+++A H G + M + +GL + ++ Sbjct: 98 AIGDSLRIVKVGDLERRYRVHVPQSYDANQATPVVIAFHGGGGNPESMIQFSGLDKKSDE 157 Query: 94 AGYMIVYPNGTGLAID-ARTWNSGGCCGYAQMHKVDDVAFLRALIDKLVKDGLADPQRIY 152 AG+++VYP GTG D T+N GGCCGYA+ +DD+ F+ AL+D L + D RI+ Sbjct: 158 AGFIVVYPYGTGQQKDRGLTFNGGGCCGYAKNRGIDDIGFVNALLDDLERAAAVDTSRIF 217 Query: 153 LVGVSNGGMMAYRMAAEAPELFKAVAVVSAVLDVPPEAVKAPMPLLHIHGSDDPFIPFLG 212 G+SNGG+M Y +A+E A+A V + K P+P++H HG+ D PF G Sbjct: 218 ATGISNGGIMTYYVASELSHRIAAIAPVGGPMMTDTCNPKRPVPVIHFHGTADGLAPFEG 277 Query: 213 GIGKQEVSQLPRI------SVARSIEAFVKADGANPRPTVTDIPDTANDGTTIRQYTYAS 266 G GK +P SV SI +++A+ +P+P V +PDTA+DG T+ + T+ + Sbjct: 278 GKGKGS-PGVPAFFRPEFNSVEYSINHWIRANDCDPQPVVEALPDTADDGMTVTRKTWGN 336 Query: 267 KSDPQAVVLYEVKGGGHAWPG--GVVPIVNGGKSSQNLDTSRNVI--NFFNAH 315 +D VVL E+K GGH WPG V P V G LD S N + FF H Sbjct: 337 GTDGAEVVLIEIKNGGHTWPGMKSVSPEVLG---ESTLDISANDLMWEFFQKH 386 >ref|YP_003371725.1| phospholipase/carboxylesterase [Pirellula staleyi DSM 6068] gb|ADB17865.1| phospholipase/Carboxylesterase [Pirellula staleyi DSM 6068] Length = 291 Score = 168 bits (426), Expect = 1e-39, Method: Composition-based stats. Identities = 90/250 (36%), Positives = 145/250 (58%), Gaps = 3/250 (1%) Query: 66 PLIVALHASGSSGSLMARATGLTEIAEAAGYMIVYPNGTGLAIDARTWNSGGC--CGYAQ 123 PL++ LH +G+ G +M TG++E+A AG+ ++YP+GTG +WN+ +A Sbjct: 36 PLLLMLHGAGTHGGVMPAFTGMSELATIAGFSVMYPSGTGRTPTTCSWNASRTRYPNFAS 95 Query: 124 MHKVDDVAFLRALIDKLVKDGLADPQRIYLVGVSNGGMMAYRMAAEAPELFKAVAVVSA- 182 VDDV F+ ID + + P+ I+ G SNGGM AYR+A E P LF+AVA V+ Sbjct: 96 RAAVDDVQFIITAIDHVREHASLAPRAIFAAGFSNGGMFAYRLAVELPSLFRAVASVAGP 155 Query: 183 VLDVPPEAVKAPMPLLHIHGSDDPFIPFLGGIGKQEVSQLPRISVARSIEAFVKADGANP 242 +LD V +P+P+ HIHG D +PF GGIG++ +S++ +V ++E + + + +P Sbjct: 156 MLDAEDAPVTSPIPVCHIHGEHDEHVPFEGGIGRKSLSKMQFPAVRETLEFWKRTNRVHP 215 Query: 243 RPTVTDIPDTANDGTTIRQYTYASKSDPQAVVLYEVKGGGHAWPGGVVPIVNGGKSSQNL 302 P + DGT++ + + + + +++Y + G GH WPG P GKS++NL Sbjct: 216 VPATAILAPLEEDGTSVTRLHFRGATTREQLLVYTIAGCGHTWPGRSSPFTILGKSTRNL 275 Query: 303 DTSRNVINFF 312 D SR + +FF Sbjct: 276 DASRAIWDFF 285 >ref|ZP_05843057.1| polyhydroxybutyrate depolymerase [Rhodobacter sp. SW2] gb|EEW25973.1| polyhydroxybutyrate depolymerase [Rhodobacter sp. SW2] Length = 291 Score = 150 bits (379), Expect = 4e-34, Method: Composition-based stats. Identities = 104/257 (40%), Positives = 143/257 (55%), Gaps = 6/257 (2%) Query: 60 AVGEPQPLIVALHASGSSGSLMARATGLTEIAEAAGYMIVYPNGTGLAIDARTWNSGGCC 119 AV P PLI+ALH G S AR GLT A AAGY + YP G+ + RTWN G CC Sbjct: 39 AVANP-PLIIALHGGGGSPRQYARDAGLTAKALAAGYAVAYPAGSARFGNLRTWNVGYCC 97 Query: 120 GYAQMHKVDDVAFL-RALIDKLVKDGLADPQRIYLVGVSNGGMMAYRMAAEAPELFKAVA 178 YA ++DD+ FL R + D + G+ +P+R+YL G+SNG MMA AA+ P+ +AVA Sbjct: 98 AYAAAARIDDIGFLDRVIADAASRYGI-NPRRVYLTGMSNGAMMAQTYAAKRPDAVRAVA 156 Query: 179 VVSAVLDVPPEAVKAPMPLLHIHGSDDPFIPFLGGIGKQEVSQLPRISVARSIEAFVKAD 238 V+ +DV + P+PLLHIHGS D +PF GG G + +V + AF +A Sbjct: 157 SVAGTMDVAHIRPQGPVPLLHIHGSADSHVPFAGGFGPDSKVETIFAAVDDVLAAFRRAQ 216 Query: 239 GANPRPTVTDIPDTANDGTTIRQYTYASKSDPQAVVLYEVKGGGHAWPGGVVPIVNGGKS 298 A TD D A DG ++ + + ++ VVL +V GGGH WPGG G + Sbjct: 217 -AGRLLAATDTIDPAPDGMSVSRTQWRDRNGVAQVVLLKVIGGGHHWPGGKRSDRRG--A 273 Query: 299 SQNLDTSRNVINFFNAH 315 +Q++D + + FF H Sbjct: 274 TQDIDAATEALRFFAQH 290 >ref|YP_004316121.1| phospholipase/carboxylesterase [Sphingobacterium sp. 21] gb|ADZ77451.1| phospholipase/Carboxylesterase [Sphingobacterium sp. 21] Length = 322 Score = 148 bits (374), Expect = 1e-33, Method: Composition-based stats. Identities = 90/250 (36%), Positives = 130/250 (52%), Gaps = 12/250 (4%) Query: 66 PLIVALHASGSSGSLMARATGLTEIAEAAGYMIVYPNG--TGLAIDARTWNSGGCCGYAQ 123 PL++ LH +G S R L+E A+ AG++ VYPNG + + RTWN+G CC +A Sbjct: 76 PLVIGLHGTGGSAVQFDRHYHLSEKADEAGFVAVYPNGVRSDGRLGIRTWNAGSCCDFAM 135 Query: 124 MHKVDDVAFLRALIDKLVKDGLADPQRIYLVGVSNGGMMAYRMAAEAPELFKAVAVVSAV 183 VDDV F+ LID+L++ D +RIY+ G+SNGGM+AYR+AAE PE A+A VS Sbjct: 136 RENVDDVEFISELIDRLIETYRIDAKRIYVTGMSNGGMLAYRLAAEIPEKIAAIAPVSCT 195 Query: 184 LDV-PPEAVKAPMPLLHIHGSDDPFIPFLGGIGKQEVSQLPRISVARSIEAFVKADGANP 242 + PP P+P++H+H D +P+ GG P SV + + K +G P Sbjct: 196 MVFDPPAEQSRPLPIIHLHSVLDEIVPYEGGTNSLGYHFAPVDSV---LNVWAKRNGCLP 252 Query: 243 RPTVTDIPDTANDGTTIRQYTYASKSDPQAVVLYEVKGGGHAWPGGVVPIVNGGKSSQNL 302 V ND T Q + ++ ++ Y GGH+WPGG S + Sbjct: 253 NAQVV----VDNDRYT--QMQWVDETGSPLIMHYVTADGGHSWPGGEQVRPGAAPPSAAI 306 Query: 303 DTSRNVINFF 312 D + + NFF Sbjct: 307 DANDLLWNFF 316