BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4701.p (436 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777133.1| major facilitator superfamily permease [Herb... 774 0.0 ref|YP_004233524.1| major facilitator superfamily protein [Acido... 578 e-163 ref|YP_969429.1| major facilitator superfamily transporter [Acid... 575 e-162 ref|YP_004683888.1| proline/betaine transporter ProP [Cupriavidu... 549 e-154 ref|YP_002943101.1| major facilitator superfamily protein [Vario... 545 e-153 >ref|YP_003777133.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] gb|ADJ65225.1| permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 436 Score = 774 bits (1998), Expect = 0.0, Method: Composition-based stats. Identities = 423/436 (97%), Positives = 431/436 (98%) Query: 1 MTTSAEFTGALNESQHRPLTSSDYRTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADM 60 MTTSAEFTGALNESQHRPL+SSDY+TLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADM Sbjct: 1 MTTSAEFTGALNESQHRPLSSSDYKTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADM 60 Query: 61 PEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTY 120 PEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTY Sbjct: 61 PEWLRMLQTFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTY 120 Query: 121 ASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLG 180 ASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLG Sbjct: 121 ASIGVAAPILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLG 180 Query: 181 SLMGIFINSVYAPAEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKQSHE 240 SLMGIFINS + PAEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRK+++E Sbjct: 181 SLMGIFINSAFEPAEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKEANE 240 Query: 241 LPLKTVLREHRGASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHLPAKLVMQANCAGTLM 300 LPLKTVLREHR ASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHL KLVMQANCAGTLM Sbjct: 241 LPLKTVLREHRAASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHLSPKLVMQANCAGTLM 300 Query: 301 LTIGCLAIGWLCDRIGTRASMIIGWGGLGLSTWWFYGQLPGSPESLIWGYALVGLFVGSI 360 LTIGCLAIGWLCDRIGTRASMIIGWGGLGLSTWWFYGQLPGSPESL+WGYALVGLFVGSI Sbjct: 301 LTIGCLAIGWLCDRIGTRASMIIGWGGLGLSTWWFYGQLPGSPESLVWGYALVGLFVGSI 360 Query: 361 SLTPIVSTRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGLSIL 420 SLTPIVSTRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGLSIL Sbjct: 361 SLTPIVSTRAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGLSIL 420 Query: 421 GVLLAFVPMSSKGYKA 436 GVLLAFVPMSSKGY+A Sbjct: 421 GVLLAFVPMSSKGYRA 436 >ref|YP_004233524.1| major facilitator superfamily protein [Acidovorax avenae subsp. avenae ATCC 19860] gb|ADX44957.1| major facilitator superfamily MFS_1 [Acidovorax avenae subsp. avenae ATCC 19860] Length = 442 Score = 578 bits (1489), Expect = e-163, Method: Composition-based stats. Identities = 311/420 (74%), Positives = 360/420 (85%) Query: 16 HRPLTSSDYRTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADMPEWLRMLQTFGIFAA 75 HRPLT DY+TLGLSALGGTLEFYDFVIYVFFA VV LFFPADMP+WLR LQTFGIFAA Sbjct: 17 HRPLTRGDYKTLGLSALGGTLEFYDFVIYVFFATVVSGLFFPADMPDWLRQLQTFGIFAA 76 Query: 76 GYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAPILLLLMR 135 GYLARP+GGI+IAH+GD +GRKRMF+LSIFLMAVPTLVIGLLPTYASIGVAAP+LLL MR Sbjct: 77 GYLARPIGGIVIAHFGDKLGRKRMFSLSIFLMAVPTLVIGLLPTYASIGVAAPLLLLAMR 136 Query: 136 TLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLGSLMGIFINSVYAPAE 195 LQGAAIGGEMPGAWVFVAEHVP NRY GVG+LTAGITGGILLGSL+ I IN Y PAE Sbjct: 137 VLQGAAIGGEMPGAWVFVAEHVPKNRYGLGVGTLTAGITGGILLGSLVAIAINRTYTPAE 196 Query: 196 VAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKQSHELPLKTVLREHRGASV 255 V G+AWRIPFILGGVFGLVSVYLRR+L ETPVF+ELAARR + ELPLKT+ R+H+GA + Sbjct: 197 VQGFAWRIPFILGGVFGLVSVYLRRFLHETPVFQELAARRAVARELPLKTIFRDHKGAML 256 Query: 256 LTAGMTWCLSGAVVVVILMTPSYLQTVHHLPAKLVMQANCAGTLMLTIGCLAIGWLCDRI 315 L A MTW LS AVVVV+L+TPSYLQ VHHLPA L ++AN TL LTIGC+ +GW DR Sbjct: 257 LVALMTWVLSTAVVVVVLITPSYLQKVHHLPATLALEANAVATLTLTIGCVVMGWATDRF 316 Query: 316 GTRASMIIGWGGLGLSTWWFYGQLPGSPESLIWGYALVGLFVGSISLTPIVSTRAFPPPV 375 GTRA M++GWGGL L+++ FY LPG+PE+L+ YALVG FVGSI+L PIV RAFPP V Sbjct: 317 GTRAVMLVGWGGLFLTSYLFYLGLPGTPETLVRNYALVGFFVGSIALLPIVGVRAFPPEV 376 Query: 376 RFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGLSILGVLLAFVPMSSKGYK 435 RF+GLSF+YNMAYAVFGG+TPVL+++W++ DP PA YV + +LG +L FVP++S+G++ Sbjct: 377 RFSGLSFSYNMAYAVFGGITPVLLTVWMQRDPMAPAHYVAAMGLLGFVLGFVPLASRGWQ 436 >ref|YP_969429.1| major facilitator superfamily transporter [Acidovorax citrulli AAC00-1] gb|ABM31655.1| major facilitator superfamily MFS_1 [Acidovorax citrulli AAC00-1] Length = 442 Score = 575 bits (1481), Expect = e-162, Method: Composition-based stats. Identities = 311/420 (74%), Positives = 359/420 (85%) Query: 16 HRPLTSSDYRTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADMPEWLRMLQTFGIFAA 75 HRPLT SDY+TLGLSALGGTLEFYDFVIYVFFA VV LFFPADMP+WLR LQTFGIFAA Sbjct: 17 HRPLTRSDYKTLGLSALGGTLEFYDFVIYVFFATVVSGLFFPADMPDWLRQLQTFGIFAA 76 Query: 76 GYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAPILLLLMR 135 GYLARP+GGI+IAH+GD +GRKRMF+LSIFLMAVPTLVIGLLPTYASIGVAAP+LLL MR Sbjct: 77 GYLARPIGGIVIAHFGDKLGRKRMFSLSIFLMAVPTLVIGLLPTYASIGVAAPLLLLAMR 136 Query: 136 TLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLGSLMGIFINSVYAPAE 195 LQGAAIGGEMPGAWVFVAEHVP NRY GVG+LTAGITGGILLGSL+ I IN Y PAE Sbjct: 137 VLQGAAIGGEMPGAWVFVAEHVPKNRYGLGVGTLTAGITGGILLGSLVAIAINRTYTPAE 196 Query: 196 VAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKQSHELPLKTVLREHRGASV 255 V G+AWRIPFILGGVFGLVSVYLRR+L ETPVF+ELAARR + ELPLKT+ R+H+GA + Sbjct: 197 VQGFAWRIPFILGGVFGLVSVYLRRFLHETPVFQELAARRAVARELPLKTIFRDHKGAML 256 Query: 256 LTAGMTWCLSGAVVVVILMTPSYLQTVHHLPAKLVMQANCAGTLMLTIGCLAIGWLCDRI 315 L A MTW LS AVVVV+L+TPSYLQ V HLPA L ++AN TL LTIGC+ +GW DR Sbjct: 257 LVALMTWVLSTAVVVVVLITPSYLQKVFHLPATLALEANAVATLTLTIGCVVMGWATDRF 316 Query: 316 GTRASMIIGWGGLGLSTWWFYGQLPGSPESLIWGYALVGLFVGSISLTPIVSTRAFPPPV 375 GTRA M+ GWGGL L+++ FY LPG+PE+L+ YALVG FVGSI+L PIV RAFPP V Sbjct: 317 GTRAVMLAGWGGLFLTSYLFYLGLPGTPETLVRNYALVGFFVGSIALLPIVGVRAFPPDV 376 Query: 376 RFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGLSILGVLLAFVPMSSKGYK 435 RF+GLSF+YNMAYAVFGG+TPVL+++W++ DP PA YV + +LG +L FVP++S+G++ Sbjct: 377 RFSGLSFSYNMAYAVFGGITPVLLTVWMQRDPMAPAHYVAAMGLLGFVLGFVPLASRGWQ 436 >ref|YP_004683888.1| proline/betaine transporter ProP [Cupriavidus necator N-1] gb|AEI75407.1| proline/betaine transporter ProP [Cupriavidus necator N-1] Length = 462 Score = 549 bits (1415), Expect = e-154, Method: Composition-based stats. Identities = 284/426 (66%), Positives = 346/426 (81%) Query: 9 GALNESQHRPLTSSDYRTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADMPEWLRMLQ 68 G + + R L + DY+TLGL+ALGGTLEFYDFVIYVFF V+G LFFP +MP+WLR LQ Sbjct: 22 GGVLPAPARTLAARDYKTLGLAALGGTLEFYDFVIYVFFVTVLGALFFPPNMPDWLRQLQ 81 Query: 69 TFGIFAAGYLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAP 128 FGIFAAGYLARP+GGI+IAH+GD +GRKRMFTLSI LMA PTL+IGLLPTYASIGVAAP Sbjct: 82 AFGIFAAGYLARPLGGIVIAHFGDRLGRKRMFTLSILLMAAPTLMIGLLPTYASIGVAAP 141 Query: 129 ILLLLMRTLQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLGSLMGIFIN 188 +LLL MR LQGAAIGGEMPGAWVFV EHVP+ Y G+G+LT+GITGGILLGSL+ + IN Sbjct: 142 LLLLAMRVLQGAAIGGEMPGAWVFVGEHVPSRHYGLGIGTLTSGITGGILLGSLVAVAIN 201 Query: 189 SVYAPAEVAGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKQSHELPLKTVLR 248 Y P +V+ +AWRIPF+LGGVFGLVSVYLRR+L ETPVFRELA R + ELP++TVLR Sbjct: 202 RHYTPEQVSDFAWRIPFLLGGVFGLVSVYLRRFLHETPVFRELADRSNLARELPIRTVLR 261 Query: 249 EHRGASVLTAGMTWCLSGAVVVVILMTPSYLQTVHHLPAKLVMQANCAGTLMLTIGCLAI 308 EHR AS+ A +TW LS ++VVV+L TP+YLQ +HH+PA L ++ N TL LTIGC+ + Sbjct: 262 EHRSASLFVALLTWVLSTSIVVVVLYTPAYLQKIHHIPAALALETNAFATLTLTIGCVIV 321 Query: 309 GWLCDRIGTRASMIIGWGGLGLSTWWFYGQLPGSPESLIWGYALVGLFVGSISLTPIVST 368 GW CDRIGTRA M+IGWGGL ++ + FYG LPG+P +L W Y L+GLFVG+I+ PI Sbjct: 322 GWACDRIGTRAVMLIGWGGLLMTAYLFYGSLPGTPGTLFWHYGLLGLFVGTIATVPIAGV 381 Query: 369 RAFPPPVRFTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGLSILGVLLAFVP 428 RAFP PVRFTGLSFAYNM+YAVFGGLTP++++LW++ D PA YV L+ +G LLAF P Sbjct: 382 RAFPAPVRFTGLSFAYNMSYAVFGGLTPMILTLWLQHDTMAPAHYVAVLAAVGSLLAFWP 441 Query: 429 MSSKGY 434 ++ +G+ Sbjct: 442 LARRGH 447 >ref|YP_002943101.1| major facilitator superfamily protein [Variovorax paradoxus S110] gb|ACS17835.1| major facilitator superfamily MFS_1 [Variovorax paradoxus S110] Length = 447 Score = 545 bits (1403), Expect = e-153, Method: Composition-based stats. Identities = 296/418 (70%), Positives = 352/418 (84%) Query: 17 RPLTSSDYRTLGLSALGGTLEFYDFVIYVFFAAVVGKLFFPADMPEWLRMLQTFGIFAAG 76 R L + DY+TL LSALGGTLEFYDFVIYVFFA V+G LFFPADMP+WLR LQTFGIFAAG Sbjct: 17 RTLDARDYKTLALSALGGTLEFYDFVIYVFFATVLGALFFPADMPDWLRQLQTFGIFAAG 76 Query: 77 YLARPVGGIIIAHYGDVIGRKRMFTLSIFLMAVPTLVIGLLPTYASIGVAAPILLLLMRT 136 YLARPVGGI+IAH+GD++GRKRMFTLSIFLMA PTLVIGLLPTYASIG+AAP+LLL MR Sbjct: 77 YLARPVGGIVIAHFGDLLGRKRMFTLSIFLMAAPTLVIGLLPTYASIGIAAPLLLLAMRV 136 Query: 137 LQGAAIGGEMPGAWVFVAEHVPANRYSFGVGSLTAGITGGILLGSLMGIFINSVYAPAEV 196 LQGAAIGGEMPGAWVF+ EHVPA+RY FG+G+LT+GITGGILLGSL+ + IN Y+PAEV Sbjct: 137 LQGAAIGGEMPGAWVFIGEHVPAHRYGFGIGALTSGITGGILLGSLVAVAINRHYSPAEV 196 Query: 197 AGYAWRIPFILGGVFGLVSVYLRRYLEETPVFRELAARRKQSHELPLKTVLREHRGASVL 256 +G+AWRIPF+LGGVFGLVSVYLRR+L ETPVF+ELA R+ + ELPL+TVLREHR ASV Sbjct: 197 SGFAWRIPFVLGGVFGLVSVYLRRFLHETPVFKELAGRKTVARELPLRTVLREHRAASVY 256 Query: 257 TAGMTWCLSGAVVVVILMTPSYLQTVHHLPAKLVMQANCAGTLMLTIGCLAIGWLCDRIG 316 A +TW LS A+VVV+L TP+YLQ VHH+PA L ++AN TL LTIGC+ GW DRIG Sbjct: 257 VALLTWVLSAAIVVVVLYTPTYLQKVHHIPAALALEANALATLTLTIGCVLAGWASDRIG 316 Query: 317 TRASMIIGWGGLGLSTWWFYGQLPGSPESLIWGYALVGLFVGSISLTPIVSTRAFPPPVR 376 TRA M++GWGGL + + FY LPG+P +L W Y+LVGLFVG+I+ PI RAFP PVR Sbjct: 317 TRAVMLVGWGGLFATAYLFYTGLPGTPATLFWHYSLVGLFVGTIATVPIAGVRAFPAPVR 376 Query: 377 FTGLSFAYNMAYAVFGGLTPVLISLWIKADPQGPALYVIGLSILGVLLAFVPMSSKGY 434 FTGLSFAYNM+YAVFGGLTPV+++LW++ D PA YV L+ LG +LA P++++G+ Sbjct: 377 FTGLSFAYNMSYAVFGGLTPVILTLWLQHDTMAPAHYVAVLAGLGFVLALWPLAARGH 434