BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4665.p (419 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777107.1| maleylacetate reductase [Herbaspirillum sero... 543 e-152 ref|YP_548891.1| iron-containing alcohol dehydrogenase [Polaromo... 469 e-130 ref|YP_001789874.1| iron-containing alcohol dehydrogenase [Lepto... 459 e-127 ref|YP_729126.1| 2-chloromaleylacetate/maleylacetate reductase [... 458 e-127 gb|AAS87585.1| maleylacetate reductase [Ralstonia sp. SJ98] 448 e-123 >ref|YP_003777107.1| maleylacetate reductase [Herbaspirillum seropedicae SmR1] gb|ADJ65199.1| maleylacetate reductase protein [Herbaspirillum seropedicae SmR1] Length = 356 Score = 543 bits (1399), Expect = e-152, Method: Composition-based stats. Identities = 311/351 (88%), Positives = 328/351 (93%) Query: 68 HSFTYTSSAARVIFGAGSLQHLVRETDLLGARRALVLCTPGQRAEAERVAALIGERCAGI 127 +FTYTS+AARVIFG GSLQHLVRET+LLGA+RALVLCTPGQRAEAERVAALIG RCAGI Sbjct: 2 QAFTYTSAAARVIFGHGSLQHLVRETELLGAQRALVLCTPGQRAEAERVAALIGARCAGI 61 Query: 128 FDQAVMHVPIETARHAREVARELGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYAG 187 FDQAVMHVPIETARHAREVAR LGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYAG Sbjct: 62 FDQAVMHVPIETARHAREVARALGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYAG 121 Query: 188 SEMTPIYGITEAGLKKTGRDARVLPRTVIYDPELSAGLPLKLSVTSGINAMAHAAEGLYA 247 SEMTPIYG+TEAG KKTGRDARVLPRTVIYDP LS GLPL LSVTSGINAMAHAAEGLYA Sbjct: 122 SEMTPIYGLTEAGRKKTGRDARVLPRTVIYDPALSLGLPLDLSVTSGINAMAHAAEGLYA 181 Query: 248 PDANPIMSLLAEEGLRALAEGLPQLVARSADLAARSQCLYGAWLCGTVLGNVGMSLHHKL 307 PDANPIMSLLAEEGLRALAEGLPQL AR+ D+ ARS+CLYGAWLCGTVLGNVGMSLHHKL Sbjct: 182 PDANPIMSLLAEEGLRALAEGLPQLAARADDIDARSRCLYGAWLCGTVLGNVGMSLHHKL 241 Query: 308 CHTLGGSYNLPHAETHTVILPHALAYNAPAAPEAMQRIARALGTDDAAQGVHALAARLQA 367 CHTLGGSYNLPHA+THTVILPHALAYNA AAP+AMQRIARALG +DAAQGV+ALA L A Sbjct: 242 CHTLGGSYNLPHAQTHTVILPHALAYNAAAAPQAMQRIARALGVEDAAQGVYALARALHA 301 Query: 368 PLSLRELGMPYEELDRCTDIALQNAYANPRPLERTALRALLENAWQGRAPG 418 PLSL+ELGMPYE L +CT+IALQNAYANPRPL+R AL ALLE+AW GR P Sbjct: 302 PLSLKELGMPYEALAQCTEIALQNAYANPRPLQREALHALLEDAWHGRPPA 352 >ref|YP_548891.1| iron-containing alcohol dehydrogenase [Polaromonas sp. JS666] gb|ABE43993.1| maleylacetate reductase [Polaromonas sp. JS666] Length = 352 Score = 469 bits (1206), Expect = e-130, Method: Composition-based stats. Identities = 255/349 (73%), Positives = 291/349 (83%) Query: 70 FTYTSSAARVIFGAGSLQHLVRETDLLGARRALVLCTPGQRAEAERVAALIGERCAGIFD 129 F YTS+ ARV+FGAGSLQ+L RE D LGAR+ LVLCTPGQR AERVAAL+ ERCAG+FD Sbjct: 4 FAYTSNPARVVFGAGSLQYLGREIDALGARKVLVLCTPGQRPLAERVAALLAERCAGVFD 63 Query: 130 QAVMHVPIETARHAREVARELGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYAGSE 189 A MHVPIETAR AREVA LGADCA+A+GGGST+GLGKAIAL+S LPILAIPTTYAGSE Sbjct: 64 GAAMHVPIETARQAREVASRLGADCAVAVGGGSTIGLGKAIALDSGLPILAIPTTYAGSE 123 Query: 190 MTPIYGITEAGLKKTGRDARVLPRTVIYDPELSAGLPLKLSVTSGINAMAHAAEGLYAPD 249 MTPIYGITEAGLKKTG+DARVLPRTV+YDPEL+ LP+ +SVTSG+NA+AHAAEGLYA D Sbjct: 124 MTPIYGITEAGLKKTGKDARVLPRTVLYDPELTTSLPVAVSVTSGLNAIAHAAEGLYAVD 183 Query: 250 ANPIMSLLAEEGLRALAEGLPQLVARSADLAARSQCLYGAWLCGTVLGNVGMSLHHKLCH 309 ANPIMSL+AEEG+ A+ LP + + DL ARS LYGAWLCGTVLG+VGM+LHHKLCH Sbjct: 184 ANPIMSLMAEEGIAAIGRSLPAIKRKPDDLQARSDALYGAWLCGTVLGHVGMALHHKLCH 243 Query: 310 TLGGSYNLPHAETHTVILPHALAYNAPAAPEAMQRIARALGTDDAAQGVHALAARLQAPL 369 TLGGS+NLPHAETHT++LPHALAYNA AAP AMQRIARAL D A V LA AP+ Sbjct: 244 TLGGSFNLPHAETHTIVLPHALAYNAKAAPVAMQRIARALKADSAPHAVFDLARDNGAPV 303 Query: 370 SLRELGMPYEELDRCTDIALQNAYANPRPLERTALRALLENAWQGRAPG 418 +L+++GM +LDR DIALQN Y NPRPLER ALR LL++A+ G P Sbjct: 304 ALKDIGMQLADLDRACDIALQNQYPNPRPLERAALRQLLQDAFDGARPA 352 >ref|YP_001789874.1| iron-containing alcohol dehydrogenase [Leptothrix cholodnii SP-6] gb|ACB33109.1| iron-containing alcohol dehydrogenase [Leptothrix cholodnii SP-6] Length = 356 Score = 459 bits (1180), Expect = e-127, Method: Composition-based stats. Identities = 248/352 (70%), Positives = 286/352 (81%) Query: 68 HSFTYTSSAARVIFGAGSLQHLVRETDLLGARRALVLCTPGQRAEAERVAALIGERCAGI 127 H F +T+ ARV+F GSL L +E + LGA R LVLCTP QR +AE A L+G G+ Sbjct: 5 HDFIFTAQPARVVFARGSLSRLPQEVEALGAHRVLVLCTPQQRDQAEAAAKLLGALSVGV 64 Query: 128 FDQAVMHVPIETARHAREVARELGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYAG 187 +D A MHVPIE AR ARE AR +GADCA+AIGGGST+GLGKAIALES LPI+A+PTTYAG Sbjct: 65 YDGAQMHVPIEGARQAREHARAVGADCAVAIGGGSTIGLGKAIALESSLPIIAVPTTYAG 124 Query: 188 SEMTPIYGITEAGLKKTGRDARVLPRTVIYDPELSAGLPLKLSVTSGINAMAHAAEGLYA 247 SEMTPIYGITEAGLKKTGRDARVLPRTV+YDP+LS GLP+ +SVTSGINA+AHAAEGLYA Sbjct: 125 SEMTPIYGITEAGLKKTGRDARVLPRTVLYDPDLSVGLPVGMSVTSGINAIAHAAEGLYA 184 Query: 248 PDANPIMSLLAEEGLRALAEGLPQLVARSADLAARSQCLYGAWLCGTVLGNVGMSLHHKL 307 D NP+MSL+AEEG+RALA GL +V +DLAAR CLYGAWLCGTVLG+VGM+LHHKL Sbjct: 185 RDGNPVMSLMAEEGIRALAIGLRGVVRSPSDLAARGDCLYGAWLCGTVLGHVGMALHHKL 244 Query: 308 CHTLGGSYNLPHAETHTVILPHALAYNAPAAPEAMQRIARALGTDDAAQGVHALAARLQA 367 CHTLGGS+NLPHAETHT++LPHALAYNA AAP AM RIA AL AA GV+ LA +L A Sbjct: 245 CHTLGGSFNLPHAETHTIVLPHALAYNASAAPIAMMRIAHALKVPSAASGVYDLAKQLGA 304 Query: 368 PLSLRELGMPYEELDRCTDIALQNAYANPRPLERTALRALLENAWQGRAPGD 419 P++LR++GMP ELDR DIAL N Y NPRP+E A+R LL NAW+GR P D Sbjct: 305 PMALRDIGMPESELDRAVDIALANPYWNPRPIEAQAIRELLRNAWEGRRPRD 356 >ref|YP_729126.1| 2-chloromaleylacetate/maleylacetate reductase [Ralstonia eutropha H16] emb|CAJ95761.1| 2-Chloromaleylacetate/maleylacetate reductase [Ralstonia eutropha H16] Length = 354 Score = 458 bits (1179), Expect = e-127, Method: Composition-based stats. Identities = 246/348 (70%), Positives = 290/348 (83%) Query: 70 FTYTSSAARVIFGAGSLQHLVRETDLLGARRALVLCTPGQRAEAERVAALIGERCAGIFD 129 F Y ++ ARV+FGAGSLQHL RE LLGA+RALVLCTP QR A+ + A +G+R AG+FD Sbjct: 7 FVYQAAPARVVFGAGSLQHLEREIGLLGAQRALVLCTPEQRDGAQAIMASLGQRAAGLFD 66 Query: 130 QAVMHVPIETARHAREVARELGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYAGSE 189 +AVMHVPI+TAR ARE+A ADCAIA GGGST+GLGKAIALES +PILAIPTTYAGSE Sbjct: 67 RAVMHVPIDTAREARELALACNADCAIAFGGGSTIGLGKAIALESSIPILAIPTTYAGSE 126 Query: 190 MTPIYGITEAGLKKTGRDARVLPRTVIYDPELSAGLPLKLSVTSGINAMAHAAEGLYAPD 249 MTPIYG+TE G+K+TG D RVLPR VIYDPEL+ LP+ LSVTSGINA+AHAAEGLYA + Sbjct: 127 MTPIYGVTEGGVKRTGSDMRVLPRAVIYDPELTLTLPVDLSVTSGINAIAHAAEGLYASN 186 Query: 250 ANPIMSLLAEEGLRALAEGLPQLVARSADLAARSQCLYGAWLCGTVLGNVGMSLHHKLCH 309 ANPI SL+AEEG+RAL +GLP + +AD+ AR Q LYGAWLCG VL NVGM+LHHKLCH Sbjct: 187 ANPITSLIAEEGIRALGQGLPGIHRDAADIVARGQALYGAWLCGMVLSNVGMALHHKLCH 246 Query: 310 TLGGSYNLPHAETHTVILPHALAYNAPAAPEAMQRIARALGTDDAAQGVHALAARLQAPL 369 TLGGS+NLPHA+THTV+LPHALA+NA AP+AMQRIARALGTD+AA+GVH LA++L AP Sbjct: 247 TLGGSFNLPHAQTHTVVLPHALAFNADFAPQAMQRIARALGTDNAARGVHDLASKLGAPT 306 Query: 370 SLRELGMPYEELDRCTDIALQNAYANPRPLERTALRALLENAWQGRAP 417 SLRELGM + +LDR +IA N Y NPRP+ R LRALL++A+ G P Sbjct: 307 SLRELGMAHADLDRAAEIASANPYWNPRPISREDLRALLQDAFDGTRP 354 >gb|AAS87585.1| maleylacetate reductase [Ralstonia sp. SJ98] Length = 351 Score = 448 bits (1152), Expect = e-123, Method: Composition-based stats. Identities = 240/350 (68%), Positives = 285/350 (81%) Query: 68 HSFTYTSSAARVIFGAGSLQHLVRETDLLGARRALVLCTPGQRAEAERVAALIGERCAGI 127 SF Y +RV+FGAGS++H+ RE + LGARRAL+L TP QR +AE +A IG R AG+ Sbjct: 2 QSFVYQGMPSRVVFGAGSIEHVEREIERLGARRALILSTPPQRDQAEALAERIGHRAAGV 61 Query: 128 FDQAVMHVPIETARHAREVARELGADCAIAIGGGSTMGLGKAIALESELPILAIPTTYAG 187 F +AVMHVP+ETAR ARE A +GADCAIAIGGGST GLGKAIAL S LPILA+PTTYAG Sbjct: 62 FAEAVMHVPVETARAAREYAERIGADCAIAIGGGSTTGLGKAIALTSSLPILAVPTTYAG 121 Query: 188 SEMTPIYGITEAGLKKTGRDARVLPRTVIYDPELSAGLPLKLSVTSGINAMAHAAEGLYA 247 SEMTPIYG+TEAGLKKTGRD RVLP+TVIYDP+L+ LP LSVTSGINA+AHAAEGLYA Sbjct: 122 SEMTPIYGLTEAGLKKTGRDMRVLPKTVIYDPQLTVSLPASLSVTSGINAIAHAAEGLYA 181 Query: 248 PDANPIMSLLAEEGLRALAEGLPQLVARSADLAARSQCLYGAWLCGTVLGNVGMSLHHKL 307 DANPI+SL+AEEG+RALA+G+ ++ D+ ARS CLYGAWLCG VLG+VGMSLHHKL Sbjct: 182 QDANPIVSLMAEEGIRALAQGIGKVARNPEDMNARSDCLYGAWLCGAVLGSVGMSLHHKL 241 Query: 308 CHTLGGSYNLPHAETHTVILPHALAYNAPAAPEAMQRIARALGTDDAAQGVHALAARLQA 367 CHTLGG++NLPHAETHT++LPHALAYN AAP AM+RIARAL DDAAQG LA A Sbjct: 242 CHTLGGTFNLPHAETHTIVLPHALAYNRDAAPGAMKRIARALNADDAAQGTFELARTNGA 301 Query: 368 PLSLRELGMPYEELDRCTDIALQNAYANPRPLERTALRALLENAWQGRAP 417 P +L+++GM ++D DIAL++ Y NPRP+ER LRAL+E A++GR P Sbjct: 302 PTALKDIGMKESDIDIAVDIALKSPYWNPRPVERAPLRALIEAAYEGRRP 351