BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4643.p (597 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777092.1| AMP-binding enzyme family protein [Herbaspir... 881 0.0 ref|YP_004752546.1| acyl-coenzyme A synthetases/AMP-(fatty) acid... 505 e-141 gb|ADU90654.1| putative AMP-dependent synthetase and ligase [Col... 490 e-136 ref|YP_586515.1| AMP-dependent synthetase and ligase [Cupriavidu... 362 1e-97 ref|YP_002030302.1| AMP-dependent synthetase and ligase [Stenotr... 351 3e-94 >ref|YP_003777092.1| AMP-binding enzyme family protein [Herbaspirillum seropedicae SmR1] gb|ADJ65184.1| AMP-binding enzyme family protein [Herbaspirillum seropedicae SmR1] Length = 586 Score = 881 bits (2276), Expect = 0.0, Method: Composition-based stats. Identities = 472/586 (80%), Positives = 511/586 (87%), Gaps = 1/586 (0%) Query: 13 MNTTAQLDLLQLPFQAFDGEHVIGWRDGTALTHAAWRAEVLRWRQLITATPGERIALFHH 72 M +LDLLQLPF AF G VIGWRDG A+THA+WRAE+ RW+ L+ TPG+R+ALFHH Sbjct: 1 MTAAPRLDLLQLPFLAFAGADVIGWRDGVAVTHASWRAELRRWQHLVQTTPGQRMALFHH 60 Query: 73 DSLHFAAALFGIWLAGKTAYLPSDTLPETCRQLRTEVDAFAGDFGAHCPALWPAADAVPD 132 D+L FAAALF IWLAGKTAYLPSD LP+TCRQL+ EVDAF G+FGAHCPAL PA DA Sbjct: 61 DTLQFAAALFAIWLAGKTAYLPSDALPDTCRQLQGEVDAFLGEFGAHCPALHPAGDAAEG 120 Query: 133 DALAPWPALRADFTGLVVYTSGSTGQAQAIPKQLGQMANEVATLEALFGSIAGQADVVAT 192 D WP LRADF GLVVYTSGSTG AQAIPKQLGQMANEVATLEALFG+I G+A+V+AT Sbjct: 121 DTPDAWPTLRADFGGLVVYTSGSTGLAQAIPKQLGQMANEVATLEALFGAIVGEAEVIAT 180 Query: 193 VSHQHIYGLLFKVLWALCAGRPIHAGSAFFPEDLQRMTGPRPWLLLSSPAHLTRLPEQVT 252 VSHQHIYGLLFKVLWALCAGRP+HAGSAFFPEDLQRM G RPW+LLSSPAHL RLPEQ Sbjct: 181 VSHQHIYGLLFKVLWALCAGRPVHAGSAFFPEDLQRMAGARPWILLSSPAHLKRLPEQGA 240 Query: 253 QPDPRQLRAIFSSGGPLPAQAARDAARLLGHRPLEIYGSSETGGIAWRQPESDLLQRREH 312 QPD RQLRAIFSSGGPLPAQAA D+ARLLG RPLEIYGSSETGGIAWRQP++D LQR+EH Sbjct: 241 QPDARQLRAIFSSGGPLPAQAAADSARLLGQRPLEIYGSSETGGIAWRQPQTDPLQRQEH 300 Query: 313 WQAMPGVAVRIGAEGALEVRSPHLPDDQWFSMADRAVLVDDLPAAFELRGRSDRIVKLEE 372 WQAMPGV VR+GAEG LEVRSPHLPD WF MADRA LV P FELRGRSDRIVKLEE Sbjct: 301 WQAMPGVEVRLGAEGELEVRSPHLPDSAWFGMADRAALVPGQPGQFELRGRSDRIVKLEE 360 Query: 373 KRISLTRIEQGLAASPLVADVRVIVHQGRRARQFIAAFVVPTAQGQAVLDGQGKLALNAA 432 KRISLTRIEQ L ASPLV DVRVIVHQGRRARQFIAAFVVP+A A+L QGKLALN A Sbjct: 361 KRISLTRIEQLLNASPLVEDVRVIVHQGRRARQFIAAFVVPSAPAHAMLAAQGKLALNTA 420 Query: 433 LKASLADAVEAIALPRLWRYLPALPLNPQGKVTLSALTALLESAGAAN-ASPPTLPRVTE 491 LKA LA A+E+IALPRLWRYLPALP+NPQGKVTLSALTALLES AA S PTLP+V+E Sbjct: 421 LKAVLAGAIESIALPRLWRYLPALPVNPQGKVTLSALTALLESPAAAPVVSAPTLPQVSE 480 Query: 492 LERTAEQVQLALEIPADLLYFQGHFPGSPILPGVVQTEWSLILGRRYFSLPPRFLGLQAL 551 LER++E VQLAL+IPADLLYF+GHFPGSPILPGVVQT+WSL LGRRYF LPP FLGLQAL Sbjct: 481 LERSSEHVQLALDIPADLLYFRGHFPGSPILPGVVQTDWSLALGRRYFDLPPHFLGLQAL 540 Query: 552 KFQRVITPGMRVVLELQHDAAKSQLAFRLHSEAGQHASGRFLLGAA 597 KFQRVITPGMRV+L+LQ +A KSQL+FRLHSE GQHASGRFLLGAA Sbjct: 541 KFQRVITPGMRVLLDLQFEAGKSQLSFRLHSEGGQHASGRFLLGAA 586 >ref|YP_004752546.1| acyl-coenzyme A synthetases/AMP-(fatty) acid ligase [Collimonas fungivorans Ter331] gb|AEK61723.1| Acyl-coenzyme A synthetases/AMP-(fatty) acid ligase [Collimonas fungivorans Ter331] Length = 604 Score = 505 bits (1301), Expect = e-141, Method: Composition-based stats. Identities = 308/572 (53%), Positives = 370/572 (64%), Gaps = 12/572 (2%) Query: 32 EHVIGWRDGTALTHAAWRAEVLRWRQLITATPGERIALFHHDSLHFAAALFGIWLAGKTA 91 E WRDG L+HA A+V WR+++++ G AL+ DS FA L G W AGKT Sbjct: 35 EQPFAWRDGKPLSHACVAAQVAAWRRVLSSQAGSCFALYSSDSATFACILLGAWQAGKTV 94 Query: 92 YLPSDTLPETCRQLRTEVDAFAGDFGAHCPALW--PAADAVPDDALAPWPALRADFTGLV 149 YLP D LP TC L VD + GDF L P A D A + AL DF LV Sbjct: 95 YLPGDVLPATCANLGKLVDGYLGDFPPQYAPLTEAPLTAAEADGARPAFKALSPDFPALV 154 Query: 150 VYTSGSTGQAQAIPKQLGQMANEVATLEALFGSIAGQADVVATVSHQHIYGLLFKVLWAL 209 +YTSGSTG+ QA+PK L Q+A EVA+LE LFG G AD+VATVSHQHIYGLLFKVLW L Sbjct: 155 IYTSGSTGEPQAVPKFLSQLAAEVASLEQLFGGRMGAADIVATVSHQHIYGLLFKVLWPL 214 Query: 210 CAGRPIHAGSAFFPEDLQRMTGPRPWLLLSSPAHLTRLPEQVTQPDPRQLRAIFSSGGPL 269 AGR IHA + + Q ++G + +L+SSPAHL RLP + +R IFSSGGPL Sbjct: 215 TAGRVIHARQLEYLNEFQPLSG-KQIVLVSSPAHLKRLPAAFSSDQAAAIRVIFSSGGPL 273 Query: 270 PAQAARDAARLLGHRPLEIYGSSETGGIAWRQPESDLLQRREHWQAMPGVAVRIGA-EGA 328 P++ A A R LG P+E+YGSSETGG+AWRQ + + WQAMP VA R+ A + Sbjct: 274 PSEVAAAAGRALGSIPIEVYGSSETGGVAWRQ---RVAGADDSWQAMPAVAWRVAANDEV 330 Query: 329 LEVRSPHLPDDQWFSMADRAVLVDDLPAAFELRGRSDRIVKLEEKRISLTRIEQGLAASP 388 LE+ SPHLPD W ++AD+ VD F L+GR DRIVK+EEKRISL +E+ L A Sbjct: 331 LEISSPHLPDAGWHALADQVQAVDR--QRFRLKGRVDRIVKVEEKRISLDGLERYLKAIT 388 Query: 389 LVADVRVIV--HQGRRARQFIAAFVVPTAQGQAVLDGQGKLALNAALKASLADAVEAIAL 446 LVAD RV++ + RQ IAAFVV +A G +L QGKLALN L+ +A AVEAIAL Sbjct: 389 LVADARVVLVEQPELQQRQRIAAFVVLSASGSEMLAAQGKLALNRLLREGIAHAVEAIAL 448 Query: 447 PRLWRYLPALPLNPQGKVTLSALTALLESAGAANASPPTL-PRVTELERTAEQVQLALEI 505 PR WRYL ALP+N QGK T + L ALLE A A +P P LE+ ++V L L + Sbjct: 449 PRSWRYLDALPVNAQGKTTRADLLALLEPAAGAGPAPAPRKPGERLLEQEPQRVLLELTV 508 Query: 506 PADLLYFQGHFPGSPILPGVVQTEWSLILGRRYFSLPPRFLGLQALKFQRVITPGMRVVL 565 PADLLYF GHF G+PILPGVVQ +W++ GRRYF+L P FLG+ LKFQR ITPGM V L Sbjct: 509 PADLLYFNGHFEGAPILPGVVQLDWAISYGRRYFALAPHFLGMLGLKFQRAITPGMVVQL 568 Query: 566 ELQHDAAKSQLAFRLHSEAGQHASGRFLLGAA 597 EL HD KS LAFRL S AGQHASGR GA Sbjct: 569 ELLHDLQKSSLAFRLFSVAGQHASGRITFGAG 600 >gb|ADU90654.1| putative AMP-dependent synthetase and ligase [Collimonas sp. MPS11E8] Length = 616 Score = 490 bits (1261), Expect = e-136, Method: Composition-based stats. Identities = 298/599 (49%), Positives = 369/599 (61%), Gaps = 42/599 (7%) Query: 32 EHVIGWRDGTALTHAAWRAEVLRWRQLITATPGERIALFHHDSLHFAAALFGIWLAGKTA 91 E WRDG L++A +V WR L+ G R AL+ DS FA L G W A KT Sbjct: 23 ERWFAWRDGEPLSYAYLGGQVAAWRGLLARQSGSRFALYSSDSATFACILLGAWQADKTI 82 Query: 92 YLPSDTLPETCRQLRTEVDAFAGDFGAHCPALWPAADAVPDDALAPWPALRA---DFTGL 148 YL D LP TC L T VD + G+F PA + DD + P + DF GL Sbjct: 83 YLAGDVLPATCASLGTLVDGYLGEF----PAQYAPLTIDSDDEIQARPEFKTLLPDFPGL 138 Query: 149 VVYTSGSTGQAQAIPKQLGQMANEVATLEALFGSIAGQADVVATVSHQHIYGLLFKVLWA 208 V+YTSGS+G+ QA+PK L Q+ANEVA+LE LFG G AD+VATVSHQHIYGLLFKVLW Sbjct: 139 VIYTSGSSGEPQAVPKFLSQLANEVASLEQLFGDRIGNADIVATVSHQHIYGLLFKVLWP 198 Query: 209 LCAGRPIHAGSAFFPEDLQRMTGPRPWLLLSSPAHLTRLPEQVTQPDPRQLRAIFSSGGP 268 L GR IHA + E Q P +L+SSPAHL RLP + TQ +R IFSSGGP Sbjct: 199 LTQGRAIHARQISYLEQFQPPRDSHPVVLVSSPAHLRRLPVEFTQSQAAAIRVIFSSGGP 258 Query: 269 LPAQAARDAARLLGHRPLEIYGSSETGGIAWRQPESDLLQRREHWQAMPGVAVRIGA-EG 327 LPA+AA A RLLG P+E+YGSSETGG+AWRQ + + WQ MP +A R+ A + Sbjct: 259 LPAEAAVAAGRLLGSIPIEVYGSSETGGVAWRQ---RIAGADDSWQVMPAIAWRVAANDE 315 Query: 328 ALEVRSPHLPDDQWFSMADRAVLVDDLPAAFELRGRSDRIVKLEEKRISLTRIEQGLAAS 387 LE+RSPHL D W ++ D+ LV P F L+GR DRIVK+EEKRISL +E+ L Sbjct: 316 LLEIRSPHLADADWHALPDQVQLVG--PQRFCLKGRVDRIVKVEEKRISLDALERELKTM 373 Query: 388 PLVADVRVIVHQ------------------GRRARQFIAAFVVPTAQGQAVLDGQGKLAL 429 VAD RV++ + + RQ IAAFVV +A G +L QGK L Sbjct: 374 AQVADARVLLVEQPESQLNAQPNAQLDSQLKTQQRQRIAAFVVLSAAGMEILAAQGKPIL 433 Query: 430 NAALKASLADAVEAIALPRLWRYLPALPLNPQGKVTLSALTALLESAGAANASPPTL--- 486 N+ L+ +A AVEA+ALPR+WRY+ ++P+N QGK T +AL ALLES + + L Sbjct: 434 NSMLRDGMAHAVEAVALPRIWRYVESMPVNAQGKTTRAALLALLESPPSGSPDLAGLALK 493 Query: 487 PRVTELERTAEQVQLALEIPADLLYFQGHFPGSPILPGVVQTEWSLILGRRYFSLPPRFL 546 P V LE+ + +V L L +PADLLYF GHF G+PILPGVVQ +W++ GR+YF+L P FL Sbjct: 494 PAVRLLEQESHRVLLELTVPADLLYFNGHFDGAPILPGVVQLDWAISYGRQYFALAPHFL 553 Query: 547 GLQALKFQRVITPGMRVVLELQH--------DAAKSQLAFRLHSEAGQHASGRFLLGAA 597 G+ LKFQRVI PGM V LEL H + KS LAFR+ S AGQHASGR GA+ Sbjct: 554 GVLGLKFQRVIIPGMVVQLELLHALKHDSVQEPPKSSLAFRMFSTAGQHASGRITFGAS 612 >ref|YP_586515.1| AMP-dependent synthetase and ligase [Cupriavidus metallidurans CH34] gb|ABF11246.1| AMP-dependent synthetase and ligase [Cupriavidus metallidurans CH34] Length = 557 Score = 362 bits (929), Expect = 1e-97, Method: Composition-based stats. Identities = 247/554 (44%), Positives = 316/554 (57%), Gaps = 30/554 (5%) Query: 50 AEVLRWRQLITATPGERIALFHHDSLHFAAALFGIWLAGKTAYLPSDTLPETCRQLRTEV 109 A WR A PG R ALF FAAALFG W AGK LP D +T LR +V Sbjct: 26 ARATAWRHAFLAAPGARWALFVDSPAEFAAALFGAWHAGKHVVLPGDARRDTLAALREQV 85 Query: 110 DAFAGDFGAHCPALWPAADAVPDDALAPWPALRADFTGLVVYTSGSTGQAQAIPKQLGQM 169 D AGD L P+A P WP L A T + ++TSGSTG +A PK+L Q+ Sbjct: 86 DGMAGDLPQ---GLQPSATESP----LQWPRLDARETWITLFTSGSTGAPEAAPKRLAQL 138 Query: 170 ANEVATLEALFG-SIAGQADVVATVSHQHIYGLLFKVLWALCAGRPIHAGSAFFPEDLQR 228 EV LE FG ++ + ++ TVSHQHIYGLLF VLW L GR + F E+L Sbjct: 139 EAEVHALEQAFGPTMPRENRLLTTVSHQHIYGLLFTVLWPLAVGRSMPVARMAFHEELVA 198 Query: 229 MTGP--RPWLLLSSPAHLTRLPEQVTQPDPRQL-RAIFSSGGPLPAQAARDAARLLGHRP 285 P +L+SSPAHL R+PE + R + RA+FSSGGPLP + A D R G P Sbjct: 199 ACAEDRTPVVLVSSPAHLKRMPEALDWAATRGVVRAVFSSGGPLPPEGASDVLRHTGVSP 258 Query: 286 LEIYGSSETGGIAWRQPESDLLQRREHWQAMPGVAVRIGAEGALEVRSPHLPDDQWFSMA 345 +E++GSSETGG+AWRQ + + W +PGVA R+ EG L VRS HL DD W A Sbjct: 259 IEVFGSSETGGVAWRQRAAG----DDRWTPLPGVAWRL-QEGFLAVRSAHLADDDWQVCA 313 Query: 346 DRAVLVDDLPAAFELRGRSDRIVKLEEKRISLTRIEQGLAASPLVADVRVIVHQGRRARQ 405 DR + D AF L GR+DRIVK+EEKR+SL+ IEQ L ASPLV + RV++ + + Sbjct: 314 DRVLPTGD--GAFVLAGRADRIVKIEEKRVSLSAIEQRLQASPLVREARVLLIETAIGTR 371 Query: 406 FIAAFVVPTAQGQAVLDGQGKLALNAALKASLADAVEAIALPRLWRYLPALPLNPQGKVT 465 AA V+ A GQ++ DG+G+ AL AAL+ +LAD V+ +ALPR W + ALP N QGK T Sbjct: 372 VGAAIVLQEA-GQSMHDGKGRHALIAALRRALADGVDRLALPRRWAFPQALPCNAQGKTT 430 Query: 466 LSALTALLESAGAANASPPTLPRVTELERTAEQVQLALEIPADLLYFQGHFPGSPILPGV 525 + L LL ++P +E L+I DL F GHFPG+P++PGV Sbjct: 431 EAMLRELLRH---------SIPVARWVEADDTSATAELDIAEDLAIFDGHFPGAPVVPGV 481 Query: 526 VQTEWSLILGRRYFSLPP--RFLGLQALKFQRVITPGMRVVLELQHDAAKSQLAFRLHSE 583 Q +W + L + ++PP RF L LKFQ V+ PG V + L A LAFRL S+ Sbjct: 482 AQVDWVMALAPQRLAVPPRQRFARLDVLKFQAVMRPGAAVRMALDWQPASCTLAFRLTSD 541 Query: 584 AGQHASGRFLLGAA 597 AG HASG+ + A Sbjct: 542 AGSHASGKIVFHPA 555 >ref|YP_002030302.1| AMP-dependent synthetase and ligase [Stenotrophomonas maltophilia R551-3] gb|ACF53619.1| AMP-dependent synthetase and ligase [Stenotrophomonas maltophilia R551-3] Length = 561 Score = 351 bits (900), Expect = 3e-94, Method: Composition-based stats. Identities = 254/564 (45%), Positives = 323/564 (57%), Gaps = 24/564 (4%) Query: 35 IGWRDGTALTHAAWRAEVLRWRQLITATPGERIALFHHDSLHFAAALFGIWLAGKTAYLP 94 +G DG + HA +R VL WR A G AL+ D++ FAAALFG W AGK +L Sbjct: 20 VGVCDGEVIDHACFRQRVLAWRAAFAAAEGRDWALYFDDAVAFAAALFGAWHAGKRVFLA 79 Query: 95 SDTLPETCRQLRTEVDAFAGDFGAHCPALWPAADAVPDDALAPWPALRADFTGLVVYTSG 154 +D LP T + L+ +V FAGD A L A A D+ L AL L V+TSG Sbjct: 80 ADNLPATLQALQPQVSGFAGDVSADYRPL-AALGATSDEGLQ---ALDERACELCVFTSG 135 Query: 155 STGQAQAIPKQLGQMANEVATLEALFGSIAGQADVVATVSHQHIYGLLFKVLWALCAGRP 214 STGQ AI K++ Q+ EV L+A FG+ V TVSHQHIYGLLF+VLW L AGR Sbjct: 136 STGQPSAINKRMDQLTREVDALQAAFGTQLEGVQVHGTVSHQHIYGLLFRVLWPLAAGRL 195 Query: 215 IHAGSAFFPEDLQRMTGPRPWLLLSSPAHLTRLPEQVTQPDPR-QLRAIFSSGGPLPAQA 273 IH FF EDL +L+++PAHL RLPEQ+ +LRA+FSSGGPLP +A Sbjct: 196 IHP-RRFFHEDLVGALAGTDTVLVATPAHLKRLPEQLDWASLHGRLRAVFSSGGPLPEEA 254 Query: 274 ARDAARLLGHRPLEIYGSSETGGIAWRQPESDLLQRREHWQAMPGVAVRIGAEGALEVRS 333 AR + LG P E+YGSSETGGIAWR+ ++DL WQ +PGV RI EG L V S Sbjct: 255 ARQVRQWLGVAPTEVYGSSETGGIAWRRWDTDL----PPWQPLPGVQWRI-EEGCLAVAS 309 Query: 334 PHLPDDQWFSMADRAVLVDDLPAAFELRGRSDRIVKLEEKRISLTRIEQGLAASPLVADV 393 HL W+ DR + D F L GR+DRIVK+EE+R+SL +E+ L V DV Sbjct: 310 AHLETPDWWRTQDRVEALAD--GRFRLLGRADRIVKIEERRVSLDALERALREDAEVDDV 367 Query: 394 RVIVHQGRRARQFIAAFVVPTAQGQAVLDGQGKLALNAALKASLADAVEAIALPRLWRYL 453 RV+V G+R + +AA VVP + AL L A LA A +A+ PR WR + Sbjct: 368 RVLVLPGQREQ--LAAVVVPADPTLLEGGEAARRALGQRLGARLAQAHDAVTRPRRWRLV 425 Query: 454 PALPLNPQGKVTLSALTALLESAGAANASPPTLPRVTELERTAEQVQLALEIPADLLYFQ 513 PALP+N QGKVT +AL AL + P +P R A L + + L FQ Sbjct: 426 PALPINAQGKVTQAALAALFQ---------PLMPVPVWDHRDAASATLRMTLDPALRPFQ 476 Query: 514 GHFPGSPILPGVVQTEWSLILGRRYFSLPPRFLGLQALKFQRVITPGMRVVLELQHDAAK 573 GHFP + ILPGV Q +W++ GR+ F++P FL + A+KFQ V PG + L+L DA + Sbjct: 477 GHFPQAAILPGVAQLDWAVRFGRQVFAMPAAFLRMDAVKFQHVARPGEELTLQLDWDATR 536 Query: 574 SQLAFRLHSEAGQHASGRFLLGAA 597 S LAFR S G HASG+ + A Sbjct: 537 SVLAFRYTSSHGVHASGKVVFADA 560