BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4585.p (225 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_03522550.1| 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldola... 228 7e-58 ref|ZP_08638467.1| 2-dehydro-3-deoxyglucarate aldolase [Halomona... 183 2e-44 ref|ZP_02155176.1| 2-dehydro-3-deoxyglucarate aldolase [Oceanibu... 157 1e-36 ref|YP_002495202.1| 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldol... 157 1e-36 ref|ZP_07952486.1| HpcH/HpaI aldolase/citrate lyase family prote... 155 3e-36 >ref|ZP_03522550.1| 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase [Rhizobium etli GR56] Length = 267 Score = 228 bits (580), Expect = 7e-58, Method: Composition-based stats. Identities = 122/212 (57%), Positives = 152/212 (71%), Gaps = 5/212 (2%) Query: 1 MEHVPLEFTDVQNLLRAVDCGSAMPIVRLASNDHTLAKRALDMGATTLMFPFVQTVEQAR 60 MEHVP+E +DV +LRA+ C A +VRLA ND L KR LD GA T+M PFVQT E+AR Sbjct: 47 MEHVPVEVSDVAGILRAIGCTPAEAVVRLAWNDQVLVKRVLDAGARTVMLPFVQTAEEAR 106 Query: 61 AAAGRGAAAAMRFPPQGTRGFAAMHRASRYGTWSEFGRRADEAVSCIVQLETPEAIARLE 120 AAA + R+PPQG RG AA+HR S++GT ++ +RA+ V IVQLETPEAI RL Sbjct: 107 AAA-----SYTRYPPQGIRGVAAVHRGSKFGTIPDYLKRANGEVCTIVQLETPEAIERLP 161 Query: 121 EIAAVPGVDALFLGPGDLSAAMGKIGNLADAEVRAAIADVARRAHAVGKPVGIVGPTPLM 180 +I+AV G+DALF+GPGDLSAAMG IGN+A EV+ I A+ AHA GKPVGIVGP P M Sbjct: 162 QISAVDGIDALFVGPGDLSAAMGHIGNIAHPEVQRLIEKAAKDAHAAGKPVGIVGPNPDM 221 Query: 181 VREFVAMGYDYVAIASDMGMMMRQANAFIAEF 212 V+ F+ GYD+ AIASD+ MM +AN ++ Sbjct: 222 VKRFLGYGYDFAAIASDIAMMTGRANDWLGSL 253 >ref|ZP_08638467.1| 2-dehydro-3-deoxyglucarate aldolase [Halomonas sp. TD01] gb|EGP18248.1| 2-dehydro-3-deoxyglucarate aldolase [Halomonas sp. TD01] Length = 267 Score = 183 bits (464), Expect = 2e-44, Method: Composition-based stats. Identities = 103/223 (46%), Positives = 148/223 (66%), Gaps = 6/223 (2%) Query: 1 MEHVPLEFTDVQNLLRAVDCGSAMPIVRLASNDHTLAKRALDMGATTLMFPFVQTVEQAR 60 MEHVP++ +++AV A +VRL ND + KR LD GA +LMFP++Q E+A Sbjct: 48 MEHVPVDVPQAIAIMQAVAGTPAELVVRLPWNDPVMVKRVLDSGAQSLMFPYIQNAEEAA 107 Query: 61 AAAGRGAAAAMRFPPQGTRGFAAMHRASRYGTWSEFGRRADEAVSCIVQLETPEAIARLE 120 A + R+P +G RG AA+HRASRYGT +++ +RADE ++ I+QLETPEA+A + Sbjct: 108 QAVN-----STRYPGKGNRGVAAVHRASRYGTVADYLQRADEEIAVILQLETPEALAAMN 162 Query: 121 EIAAVPGVDALFLGPGDLSAAMGKIGNLADAEVRAAIADVARRAHAVGKPVGIVGPTPLM 180 +IAAV +D LF+GPGDLSAAMG+IG++ AEV++AI+ + +G P GIVG P + Sbjct: 163 DIAAVENIDGLFVGPGDLSAAMGRIGDIRHAEVQSAISAAVADSQRLGLPCGIVGAEPGL 222 Query: 181 VREFVAMGYDYVAIASDMGMMMRQANAFIAEFD-QTAPQANAG 222 V + A G+ +VA+ SDM MMM +A ++A Q A A++G Sbjct: 223 VSSYRAQGFSFVAVGSDMSMMMSRAKEYVAALKGQVADTADSG 265 >ref|ZP_02155176.1| 2-dehydro-3-deoxyglucarate aldolase [Oceanibulbus indolifex HEL-45] gb|EDQ03304.1| 2-dehydro-3-deoxyglucarate aldolase [Oceanibulbus indolifex HEL-45] Length = 266 Score = 157 bits (397), Expect = 1e-36, Method: Composition-based stats. Identities = 93/220 (42%), Positives = 129/220 (58%), Gaps = 11/220 (5%) Query: 1 MEHVPLEFTDVQNLLRAVDCGSAMP----IVRLASNDHTLAKRALDMGATTLMFPFVQTV 56 MEH P + + + + LRA GSA P +VR+ S+D T+ KR LD+G LMFP +Q Sbjct: 44 MEHAPNDLSQIIDHLRAA--GSANPDAQMVVRMPSSDQTMTKRLLDIGVRNLMFPMIQNA 101 Query: 57 EQARAAAGRGAAAAMRFPPQGTRGFAAMHRASRYGTWSEFGRRADEAVSCIVQLETPEAI 116 E AR A A R+PP+G RG +A RA+ Y +E+ +A IVQ+ETPEA+ Sbjct: 102 EDARRAM-----AWTRYPPEGLRGISATIRANNYARQTEYLNTYADAQCVIVQVETPEAV 156 Query: 117 ARLEEIAAVPGVDALFLGPGDLSAAMGKIGNLADAEVRAAIADVARRAHAVGKPVGIVGP 176 A + IAAVPGVDA+F+GPGDLS+AMG GN +V+ I + HA G P GI+G Sbjct: 157 AEISAIAAVPGVDAIFIGPGDLSSAMGHTGNPMHPDVQDKIREAVDAIHAAGLPAGILGY 216 Query: 177 TPLMVREFVAMGYDYVAIASDMGMMMRQANAFIAEFDQTA 216 + R + G ++VAIA D ++ RQ +A + Q + Sbjct: 217 GADLARRYFDGGIEFVAIAGDAWLLTRQMDALVQTMAQPS 256 >ref|YP_002495202.1| 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase [Methylobacterium nodulans ORS 2060] gb|ACL62710.1| 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase [Methylobacterium nodulans ORS 2060] Length = 255 Score = 157 bits (397), Expect = 1e-36, Method: Composition-based stats. Identities = 93/211 (44%), Positives = 132/211 (62%), Gaps = 5/211 (2%) Query: 2 EHVPLEFTDVQNLLRAVDCGSAMPIVRLASNDHTLAKRALDMGATTLMFPFVQTVEQARA 61 EH P + V L+AV P+VR ASND L KR LD+GA TL+ P+VQ +A+A Sbjct: 47 EHSPGDPLTVLAQLQAVAPYDVAPVVRPASNDTVLIKRFLDLGAQTLLIPYVQDAHEAQA 106 Query: 62 AAGRGAAAAMRFPPQGTRGFAAMHRASRYGTWSEFGRRADEAVSCIVQLETPEAIARLEE 121 A AA R+PP+G RG + + RA+R+G + + +RA E + +VQ+ET EA+ RLEE Sbjct: 107 AV-----AATRYPPEGVRGVSGLTRATRFGRVAAYAKRASEEICVLVQVETREALDRLEE 161 Query: 122 IAAVPGVDALFLGPGDLSAAMGKIGNLADAEVRAAIADVARRAHAVGKPVGIVGPTPLMV 181 IAAV GVD +F+GP DL+A++G +G EV +A+ D RR A GKP GI+ P P Sbjct: 162 IAAVEGVDGIFVGPADLAASLGHVGEPGHPEVVSAVEDAVRRIRASGKPAGILTPDPDFA 221 Query: 182 REFVAMGYDYVAIASDMGMMMRQANAFIAEF 212 R +A+G + A+A D+G++ R A + +F Sbjct: 222 RRCIALGTTFTAVAIDVGVLARGTEAVMRQF 252 >ref|ZP_07952486.1| HpcH/HpaI aldolase/citrate lyase family protein [Enterobacteriaceae bacterium 9_2_54FAA] gb|EFV39186.1| HpcH/HpaI aldolase/citrate lyase family protein [Enterobacteriaceae bacterium 9_2_54FAA] Length = 263 Score = 155 bits (393), Expect = 3e-36, Method: Composition-based stats. Identities = 91/211 (43%), Positives = 129/211 (61%), Gaps = 7/211 (3%) Query: 2 EHVPLEFTDVQNLLRAVDCGSA--MPIVRLASNDHTLAKRALDMGATTLMFPFVQTVEQA 59 EHV ++ D + RA+ + P+ RLA N+ L KRALD GA +LM PF+ TVE+A Sbjct: 43 EHVAVDTLDAIRIDRAIRSVNPRITPLYRLAGNETVLIKRALDGGAMSLMIPFINTVEEA 102 Query: 60 RAAAGRGAAAAMRFPPQGTRGFAAMHRASRYGTWSEFGRRADEAVSCIVQLETPEAIARL 119 + A + +PP G RGFAAMHRAS +G+ +F + + + I+Q+ETPEAI + Sbjct: 103 QKAI-----ESAYYPPYGKRGFAAMHRASIWGSNPDFVAQGRDDLCLILQMETPEAIGNI 157 Query: 120 EEIAAVPGVDALFLGPGDLSAAMGKIGNLADAEVRAAIADVARRAHAVGKPVGIVGPTPL 179 EIA + GV F+GPGDLSAAMG GN + EV+A I +GKP GIVG P Sbjct: 158 AEIAKLEGVTGFFIGPGDLSAAMGYPGNPSHPEVQAMIKKGVDACVRLGKPCGIVGGNPE 217 Query: 180 MVREFVAMGYDYVAIASDMGMMMRQANAFIA 210 + ++ +MG+ +VA+ASDM ++ +A +A Sbjct: 218 LANKYQSMGFRFVALASDMSFLLARAKEQLA 248