BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4545.p (303 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777036.1| Tfp pilus assembly transmembrane PilW protei... 324 1e-86 ref|YP_001100629.1| putative Tfp pilus assembly protein PilW [He... 138 1e-30 ref|YP_001354128.1| PilW type IV pilus assembly protein [Janthin... 134 3e-29 ref|YP_295005.1| transmembrane protein [Ralstonia eutropha JMP13... 122 5e-26 ref|YP_002980685.1| hypothetical protein Rpic12D_0712 [Ralstonia... 108 1e-21 >ref|YP_003777036.1| Tfp pilus assembly transmembrane PilW protein [Herbaspirillum seropedicae SmR1] gb|ADJ65128.1| Tfp pilus assembly transmembrane PilW protein [Herbaspirillum seropedicae SmR1] Length = 294 Score = 324 bits (831), Expect = 1e-86, Method: Composition-based stats. Identities = 183/287 (63%), Positives = 214/287 (74%), Gaps = 3/287 (1%) Query: 16 ETGLTLVELMIALAIGLLVVLVAGTLLQQARAAYQEMDDAGRVEETARVALDHLQQVLRQ 75 + GLTLVELM+ALA+GLLVVL AG LLQQAR +Y+++DDA RV ET R+ LDHLQQ LRQ Sbjct: 10 QAGLTLVELMVALAVGLLVVLAAGVLLQQARESYEDIDDASRVNETGRLVLDHLQQALRQ 69 Query: 76 AAHLPWETLDGKTAPTLTAGLRGLDDSGQAELLDPATGLFGSSTGDGVNHSDLLMLGFFG 135 A HLPWE L + P L AGLRG+DDS QAEL DPATG FG STGDG++HSDLLMLG FG Sbjct: 70 AGHLPWEDL--RQPPALAAGLRGVDDSRQAELHDPATGQFGGSTGDGLHHSDLLMLGLFG 127 Query: 136 APRGSHAEISHCSGAQAPVGKPEEAARHWVIYYIAPGTGDEPELRCRYQGKNGNWTSDAI 195 A S A++ +CSGA AP G EE+ARHW+IYYIA G+ EPELRCRYQG++G WTSDAI Sbjct: 128 AAPASRAQVGNCSGAPAPTGPLEESARHWMIYYIAAGSSGEPELRCRYQGRHGVWTSDAI 187 Query: 196 ARGVEAMQLRYAIDSDGDGRPDRWLDASAMPGEAWGRVILVRIALLVRGVQRRPAAPGSE 255 ARGVEAMQLRYAIDSD DG PD+W DAS M W +V+LV+IALLVRG ++RP S Sbjct: 188 ARGVEAMQLRYAIDSDSDGAPDQWRDASDMSAAQWSQVVLVQIALLVRGERQRPRDVVS- 246 Query: 256 TRSYDLFLPGRRSDPAWRVTETGQEQRRRAVFQGSVMLRNQSMAEAG 302 R Y+L + P W V E RRRAVF+ +V+LRN AG Sbjct: 247 PRRYELLAAPPPTPPGWTVDAAPGEDRRRAVFRATVLLRNLRSPGAG 293 >ref|YP_001100629.1| putative Tfp pilus assembly protein PilW [Herminiimonas arsenicoxydans] emb|CAL62508.1| Putative Tfp pilus assembly protein PilW [Herminiimonas arsenicoxydans] Length = 315 Score = 138 bits (348), Expect = 1e-30, Method: Composition-based stats. Identities = 108/319 (33%), Positives = 158/319 (49%), Gaps = 49/319 (15%) Query: 19 LTLVELMIALAIGLLVVLVAGTLLQQARAAYQEMDDAGRVEETARVALDHLQQVLRQAAH 78 +TL+ELMIA+ +GL +VL A LL ++ Y D ++ET R A++ L + +RQA + Sbjct: 1 MTLIELMIAITLGLFLVLTAIGLLVASKTGYVMQDQQAHIQETGRYAIEILSRAVRQAGY 60 Query: 79 LPWE--TLDGKTAPTLTAGLRGLDDSGQAELLDPATGLFGSSTG-DGVNHSDLLMLGFFG 135 W T+ + +A + G D A L T S T D VN SD+L L FFG Sbjct: 61 ENWHSATMPAQVEAAFSASITGRD----AMTLKKTTPELDSLTSTDVVNGSDVLALRFFG 116 Query: 136 -APRGSHAEISHCSG---AQAPVGKPEEAARHWVIYYIAPGTGDEPELRCRYQGKNGNWT 191 G I +C+G A E R W I+++A + EPELRC+YQ K +W Sbjct: 117 DDADGEEGAILNCAGLPVAATSAATGNEQERGWSIFFVAKDSAGEPELRCKYQSKT-SWN 175 Query: 192 SDAIARGVEAMQLRYAIDSDGDGRPDRWLDASA---------MPGEA------------- 229 +DAIARGVE+ Q+ Y +D+DGDG +++L+A + GE Sbjct: 176 ADAIARGVESFQVLYGLDADGDGVAEQFLNADGLNALDARLLLSGENALARAGDFNRQTH 235 Query: 230 WGRVILVRIALLVRGVQ--RRPAAPGSETRSYDLFLPG-----RRSDPAWRVTET----G 278 W ++ ++++ LLVRG Q R A G YDLF SD R++E+ Sbjct: 236 WKKITVIKLGLLVRGSQVARDDALRG----EYDLFGAAYGDMHAASDKGSRISESALPAA 291 Query: 279 QEQRRRAVFQGSVMLRNQS 297 + R R VF ++ LRN + Sbjct: 292 AKNRIRKVFTHTIQLRNAA 310 >ref|YP_001354128.1| PilW type IV pilus assembly protein [Janthinobacterium sp. Marseille] gb|ABR89296.1| PilW type IV pilus assembly protein [Janthinobacterium sp. Marseille] Length = 305 Score = 134 bits (336), Expect = 3e-29, Method: Composition-based stats. Identities = 107/312 (34%), Positives = 156/312 (50%), Gaps = 51/312 (16%) Query: 21 LVELMIALAIGLLVVLVAGTLLQQARAAYQEMDDAGRVEETARVALDHLQQVLRQAAHLP 80 ++ELMIAL++GL V+L A LL +AAY D +V+ET A++ L + +RQA Sbjct: 1 MIELMIALSLGLFVILSATGLLLSGKAAYLAQDQNAQVQETGPYAIEMLSRAIRQAG--- 57 Query: 81 WETLDGKTAPT-----LTAGLRGLDDSGQAELLDPATGLFGSSTGDGVNHSDLLMLGFFG 135 +E DG P + + G+D L L ++ VN SD++ L FF Sbjct: 58 YENRDGDGTPIRRTADFSPDIMGMD---ARSLKRTGPALAEAAPSATVNGSDVMALRFF- 113 Query: 136 APRGSHAEISHCSGAQAPVGKPEE---AARHWVIYYIAPGTGDEPELRCRYQGKNGNWTS 192 G I +C+G P ++ AR W I+Y+A +G EPELRC+Y+ + G W++ Sbjct: 114 ---GDGEVIVNCAGLIVPAAASQQHADEARGWSIFYVARDSGGEPELRCKYRSQKG-WSA 169 Query: 193 DAIARGVEAMQLRYAIDSDGDGRPDRWLDAS-------AMP--GEA-------------W 230 DAIARGVE+ Q+ Y +D DGDG DR+++AS AMP GE W Sbjct: 170 DAIARGVESFQVLYGVDVDGDGLADRFVNASDIDALDDAMPLTGENALARAADKNRRTYW 229 Query: 231 GRVILVRIALLVRGVQRRPAAPGSETRSYDLFLPG---RRSDPAWRVTET----GQEQRR 283 R+ +R++LL+R Q AA G R R D R+ E ++R Sbjct: 230 KRIRSIRVSLLMRSEQ---AARGDVPREVHYLFGSAYDRIDDKGTRIDEANLSPAVQKRI 286 Query: 284 RAVFQGSVMLRN 295 R VF +++LRN Sbjct: 287 RKVFTQTILLRN 298 >ref|YP_295005.1| transmembrane protein [Ralstonia eutropha JMP134] gb|AAZ60161.1| probable transmembrane protein [Ralstonia eutropha JMP134] Length = 310 Score = 122 bits (307), Expect = 5e-26, Method: Composition-based stats. Identities = 107/313 (34%), Positives = 150/313 (47%), Gaps = 34/313 (10%) Query: 1 MPARSPFPARAMRLHETGLTLVELMIALAIGLLVVLVAGTLLQQARAAYQEMDDAGRVEE 60 M +R P R G TL E M+ LA+G+LV VAGT RAAY + +EE Sbjct: 5 MRSRMPAGYRRSGPRMEGFTLTETMVGLALGVLVTAVAGTAFLLCRAAYLSAAERTLLEE 64 Query: 61 TARVALDHLQQVLRQAAHLPWETLDGKTAPTLTAGLRGLDDSGQAELLDPATGLFGSSTG 120 + AL+ L ++RQ+ +P D P + G DD GQ P+ + Sbjct: 65 RGQRALNILSALIRQSGWVP----DATGMPHAKPAISGADDCGQ-----PSIATVPACGK 115 Query: 121 DGVNHSDLLMLGFFGAPRGSHAEIS-----HCSGAQAPVGKPEEAARHWVI----YYIAP 171 GV SD L++ F G+ + A + CSG + + A + Y+A Sbjct: 116 SGVTGSDALLVRFSGSGQAIDASLPDQTMIDCSGYAVAARQSDSPADAGYVTANLLYVAT 175 Query: 172 GTGDEPELRCRYQG-KNG-----NWTSDAIARGVEAMQLRYAIDSDGDGRPDRWLDA--- 222 + DEP+L CRY +NG WTS AI RGVE+MQ+RY +DS GD PD +L A Sbjct: 176 ASDDEPQLLCRYPARRNGRIDGSGWTSGAIVRGVESMQIRYGVDSRGDRLPDTFLRADQV 235 Query: 223 SAMPGEAWGRVILVRIALLVRGVQRRPAAPGSETRSYDLFLPGRRSDPAWRVTETGQEQR 282 +AM +AW RV+ V+IAL VR +R A G + G +D R++ G+ Sbjct: 236 TAMGDDAWHRVVAVQIALAVRLGRRGAAQSGMQVAG-----AGAMTDLYDRLSPQGRYTS 290 Query: 283 RRAVFQGSVMLRN 295 R +F +V LRN Sbjct: 291 R--IFATTVRLRN 301 >ref|YP_002980685.1| hypothetical protein Rpic12D_0712 [Ralstonia pickettii 12D] ref|ZP_07675566.1| prepilin-type N [Ralstonia sp. 5_7_47FAA] gb|ACS62013.1| conserved hypothetical protein [Ralstonia pickettii 12D] gb|EFP66163.1| prepilin-type N [Ralstonia sp. 5_7_47FAA] Length = 310 Score = 108 bits (270), Expect = 1e-21, Method: Composition-based stats. Identities = 100/298 (33%), Positives = 140/298 (46%), Gaps = 26/298 (8%) Query: 18 GLTLVELMIALAIGLLVVLVAGTLLQQARAAYQEMDDAGRVEETARVALDHLQQVLRQAA 77 G TL EL+I +GL+V+ +A L ARA+YQ + D +E AL+ L L+ A Sbjct: 10 GATLAELLIGTLVGLIVLGIALQLTLLARASYQRVADDALIESRGTQALELLTMALQHAG 69 Query: 78 HLPWETLDGKTAPT--LTAGLRGLDDSGQAELLDPATGLFGSSTGDGVNHSDLLMLGFFG 135 W T +P L G P T + G + G G+ +SD +++ F G Sbjct: 70 ---WATDTPAASPERRWPDAKATLSIQGADSCTPPETEVLGCN-GSGIRNSDAVLVRFAG 125 Query: 136 A-----PRGSHAEISHCS--GAQAPVGKPEEAARH-WVIYYIAPGTGDEPELRCRY---- 183 P+ + I CS G PV + R +++ Y+A T EP+L CR Sbjct: 126 RSMHANPQRADDSIIDCSGYGVAEPVQNGGDDQRAGYMLLYLARSTDQEPQLMCRSRRHQ 185 Query: 184 --QGKNGNWTSDAIARGVEAMQLRYAIDSDGDGRPDRWLDASAMPGEAWGRVILVRIALL 241 Q G WTS+++ RGVE MQL Y + S G G L A ++ E W +V V IAL+ Sbjct: 186 AGQTVGGRWTSNSMVRGVETMQLLYVVRSPG-GTSSTTLSARSLTTEQWRQVQAVHIALV 244 Query: 242 VRGVQRRPAAPGSETRSYDLFLPGRRSDPA----WRVTETGQEQRRRAVFQGSVMLRN 295 VRG P + T + LF +R + A T T + QRRRAVF +V LRN Sbjct: 245 VRGEYNTPRSANKATSTLALFPDLKRVEGADAQDLHFTPT-EPQRRRAVFTATVRLRN 301