BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4442.p (456 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004751487.1| membrane spanning protein involved in lipopo... 209 6e-52 ref|YP_294983.1| O-antigen polymerase [Ralstonia eutropha JMP134... 184 3e-44 ref|YP_004686613.1| membrane spanning protein involved in lipopo... 177 3e-42 ref|YP_727325.1| membrane spanning protein involved in lipopolys... 176 1e-41 ref|YP_002981958.1| O-antigen polymerase [Ralstonia pickettii 12... 170 5e-40 >ref|YP_004751487.1| membrane spanning protein involved in lipopolysaccharide biosynthesis [Collimonas fungivorans Ter331] gb|AEK60664.1| membrane spanning protein involved in lipopolysaccharide biosynthesis [Collimonas fungivorans Ter331] Length = 417 Score = 209 bits (533), Expect = 6e-52, Method: Composition-based stats. Identities = 136/357 (38%), Positives = 201/357 (56%), Gaps = 6/357 (1%) Query: 75 FKDLMRRYWYVHLGMAALFIATVIGAGFNGFNSPKVFDEPSRLAVFPLVLWMLLSLSPRQ 134 F L++ YW + L M A IA +G + +D P RLA+F LV W++ L R Sbjct: 52 FAQLVKDYWPLGLAMCAPLIAVFANQAASGNFLGRTYDPPFRLAMFVLVFWIVSFLPIRY 111 Query: 135 VKVAQAGYVLGAIVGAVLIFVATKGGENRNNIHGAPIIVFSNIVLLLGMLSVATSTSSQS 194 +K Q V+GA + A+ I++ T GG +R PII+F+ + LLLG+ SV + ++ Sbjct: 112 MKHVQWAIVIGAFLSAIKIYILTDGGASRYGTDFIPIIIFAEMALLLGLFSVFSIAWNKP 171 Query: 195 ESWPWRVLRIVAALAGMYASYLSQARGSWIAIPFLGLFASYLWQ--PNKRYVTTVVAAVL 252 + ++ A AG+Y +YLSQ+RG W+ IP + A + + P ++ +V V+ Sbjct: 172 GNKLAIFIKFAALCAGLYVAYLSQSRGVWLTIPVFIILAFLITKNIPTSYKLSVIVFFVI 231 Query: 253 LTGALVLAWSSPMVKSRFLQVETETASFRSGTDLDTSLGTRFQLWHGSMVLFTEHPLLGV 312 L G ++ +VK R E++ + G ++DTSLG R QLW GS VLFTEHP+ GV Sbjct: 232 LIGG--VSHYGQIVKERVSVAESDMTQYSGGENVDTSLGIRLQLWRGSWVLFTEHPVFGV 289 Query: 313 GPGKFTAGLRELKERKIITPAAASGFYHSHNDVLFNMSTMGLLGLLAILMTYLAPAVLFW 372 G F L L ER IITP AA+ + HSHN+VLF MS +GL GL A+L Y PA F Sbjct: 290 GVEGFPKALEGLAERNIITPVAAT-YPHSHNEVLFMMSRLGLFGLFALLALYFVPAYYFL 348 Query: 373 RYLRHEDACLRRYAGGGVMLVMGYVIFGLTD-CFFWGLSYTFYCIAVATLAAPLIQS 428 R +++ D +R AG GV L +G+V+ GL D F W Y +Y +++A +++S Sbjct: 349 RDIQNRDHEIRYTAGMGVALCLGFVVLGLVDVVFLWWEIYPYYVLSIAFFLTYIMKS 405 >ref|YP_294983.1| O-antigen polymerase [Ralstonia eutropha JMP134] gb|AAZ60139.1| O-antigen polymerase [Ralstonia eutropha JMP134] Length = 430 Score = 184 bits (467), Expect = 3e-44, Method: Composition-based stats. Identities = 132/417 (31%), Positives = 224/417 (53%), Gaps = 7/417 (1%) Query: 27 LFDAFVTLLLLLVFPAL-LMMTRGGNAGFYLLLVCALVHVFL-VRRHRIPFKDLMRRYWY 84 LF + V+ LLL +PA+ L + GNA +LLL+ ++ + + ++ + F +L++ YW Sbjct: 16 LFQSLVSALLL-AYPAIALFVHNAGNAILFLLLILSVTAMTIRLQPDGVGFGELLKAYWP 74 Query: 85 VHLGMAALFIATVIGAGFNGFNSPKVFDEPSRLAVFPLVLWMLLSLSPRQVKVAQAGYVL 144 +HL MA+ +A I + G + + +D RLA+FP V W+L + R +K+ Q ++ Sbjct: 75 LHLAMASGCLAIFINQLWTGEFAARDYDRALRLAIFPFVFWILFFVPIRHLKLLQWSFLA 134 Query: 145 GAIVGAVLIFVATKGGENRN-NIHGAPIIVFSNIVLLLGMLSVATSTSSQSESWPWRVLR 203 A++ ++ T GG+ R+ NI II +S+I LL G+L V + ++ + +++ Sbjct: 135 AAVIAMAKAYIFTNGGDVRDGNIGFISIIAYSDIALLAGVLCVVSFGWNEGKLKLVLLVK 194 Query: 204 IVAALAGMYASYLSQARGSWIAIPFLGLFASYLWQPNKRYVTTVVAAVLLTGALVLAWSS 263 I+A +AG+Y S L+ RGSW+AIP +F + + R VA+ ++ L+L + Sbjct: 195 ILACVAGLYTSVLTATRGSWLAIPLFLIFFLFFARLEVRRKWLWVASFVVVVGLMLTLNQ 254 Query: 264 PMVKSRFLQVETETASFRSGTDLDTSLGTRFQLWHGSMVLFTEHPLLGVGPGKFTAGLRE 323 +RFL +++ A++ G TS G R QLW ++ L P+ G+G + + ++E Sbjct: 255 N-AANRFLDTQSDLAAYSHGGGKSTSTGHRLQLWGAALTLIAREPVFGIGRENYDSSIKE 313 Query: 324 LKERKIITPAAASGFYHSHNDVLFNMSTMGLLGLLAILMTYLAPAVLFWRYLRHEDACLR 383 + +K IT + F HSHN++LF+ G+ GLL+ L YL P F R L + +R Sbjct: 314 MARKKEIT-GELTRFAHSHNEILFSTVVSGVFGLLSALALYLVPGYFFARELSNTAPDIR 372 Query: 384 RYAGGGVMLVMGYVIFGLTDC-FFWGLSYTFYCIAVATLAAPLIQSTLQQRAATRQG 439 A G LV+G+ FGLTD FFW + +Y I + +I+S Q + Q Sbjct: 373 TAARMGCALVIGFFSFGLTDLMFFWPVLGGYYSIMIGAFLVFIIRSKAQPSQLSPQA 429 >ref|YP_004686613.1| membrane spanning protein involved in lipopolysaccharide biosynthesis [Cupriavidus necator N-1] gb|AEI78132.1| membrane spanning protein involved in lipopolysaccharide biosynthesis [Cupriavidus necator N-1] Length = 429 Score = 177 bits (449), Expect = 3e-42, Method: Composition-based stats. Identities = 137/395 (34%), Positives = 209/395 (52%), Gaps = 7/395 (1%) Query: 36 LLLVFPALLMMTRGGNAGFYLLLV--CALVHVFLVRRHRIPFKDLMRRYWYVHLGMAALF 93 +L FPAL+ + + Y LL+ A+ V R R L+RRYW L MAAL Sbjct: 26 VLAAFPALMFVKPTVSNLCYGLLLGWSAITLVAGGRAGRAECGALLRRYWLFMLAMAALP 85 Query: 94 IATVIGAGFNGFNSPKVFDEPSRLAVFPLVLWMLLSLSPRQVKVAQAGYVLGAIVGAVLI 153 +A ++ G P V R A+F +++ ++ L ++ Q G+V+ A++ A I Sbjct: 86 LAVLLQQLLAGAEEPHVSYLYLRFALFIPLVFGMVRLGRGGMRSVQWGFVVCALLSAAWI 145 Query: 154 FVATKGGENRNNIHGAPIIVFSNIVLLLGMLSVATSTSSQSESWPWRVLRIVAALAGMYA 213 AT G + + + II SN+ +L+G+L++ + + + L+++A AG+YA Sbjct: 146 HEATAAGRPAH-VGFSNIIPLSNLSMLMGILALISIGWDRPRGYLQSGLKLLAGSAGLYA 204 Query: 214 SYLSQARGSWIAIPFLGLFASYLWQPNKRYVTTVVAAVLLTGALVLAW-SSPMVKSRFLQ 272 SY S RG W+AIP L L A + R V V A L G +VL W SP V+ R Sbjct: 205 SYTSGTRGGWLAIPVLALIALAASRRLTR-VHKVAALAGLAGLMVLTWFGSPFVQQRTTD 263 Query: 273 VETETASFRSGTDLDTSLGTRFQLWHGSMVLFTEHPLLGVGPGKFTAGLRELKERKIITP 332 V +E + F LDTS+G R QLW S+ + HP GVGP + L+ L + +IT Sbjct: 264 VVSELSQFDHHVTLDTSIGIRLQLWRTSLQMVQAHPWTGVGPDNYEQALQALAAQHVITA 323 Query: 333 AAASGFYHSHNDVLFNMSTMGLLGLLAILMTYLAPAVLFWRYLRHEDACLRRYAGGGVML 392 AA+ H+HND+L +T+G+ GLLA+LM YL PA F R+LR++D + + G+ L Sbjct: 324 EAAT-MPHTHNDILHAAATLGIPGLLAVLMLYLVPAAFFLRHLRNDDRDTQVASAMGLAL 382 Query: 393 VMGYVIFGLTDCFF-WGLSYTFYCIAVATLAAPLI 426 G+++FGLT+ F L FY + +A A ++ Sbjct: 383 CCGFMVFGLTEVMFATTLVNAFYSLIMAVCFAYVV 417 >ref|YP_727325.1| membrane spanning protein involved in lipopolysaccharide biosynthesis [Ralstonia eutropha H16] emb|CAJ93957.1| membrane spanning protein involved in lipopolysaccharide biosynthesis [Ralstonia eutropha H16] Length = 429 Score = 176 bits (445), Expect = 1e-41, Method: Composition-based stats. Identities = 138/407 (33%), Positives = 211/407 (51%), Gaps = 9/407 (2%) Query: 36 LLLVFPALLMMTRGGNAGFYLLLV--CALVHVFLVRRHRIPFKDLMRRYWYVHLGMAALF 93 +L FPAL+ + + Y LL+ A+ V R R L+RRYW+ L MAAL Sbjct: 26 VLAAFPALMFVKPTVSNLCYGLLLGWSAITLVAGGREGRTECGGLLRRYWFFMLAMAALP 85 Query: 94 IATVIGAGFNGFNSPKVFDEPSRLAVFPLVLWMLLSLSPRQVKVAQAGYVLGAIVGAVLI 153 +A ++ +G P V R A+F +++ ++ L ++ Q G+V+ A++ A I Sbjct: 86 LAVLLQQLLSGAEEPHVSYLYLRFALFIPLVFGMVRLGRGGMRSVQWGFVVCALLSAAWI 145 Query: 154 FVATKGGENRNNIHGAPIIVFSNIVLLLGMLSVATSTSSQSESWPWRVLRIVAALAGMYA 213 AT G + + + II SN+ +L+G+L++ + + + L+++A AG+YA Sbjct: 146 HEATAAGRPVH-VGSSNIIPLSNLSMLMGILALISIGWDRPRDYLQSGLKLLAGFAGLYA 204 Query: 214 SYLSQARGSWIAIPFLGLFASYLWQPNKRYVTTVVAAVLLTGALVLAW-SSPMVKSRFLQ 272 SY+S RG W+AIP L A R + A V L G + LAW S V+ R Sbjct: 205 SYMSGTRGGWLAIPVLAFIALAASHRLTR-MHKAAAMVGLAGLMGLAWLGSARVQQRTAD 263 Query: 273 VETETASFRSGTDLDTSLGTRFQLWHGSMVLFTEHPLLGVGPGKFTAGLRELKERKIITP 332 V +E + F DT G R QLW S+ L HP GVGP F L+ L+ + +ITP Sbjct: 264 VVSEVSQFAQHVTPDTPTGIRLQLWRASLELIQAHPWTGVGPENFERTLQTLEAQHVITP 323 Query: 333 AAASGFYHSHNDVLFNMSTMGLLGLLAILMTYLAPAVLFWRYLRHEDACLRRYAGGGVML 392 AA+ HSHN++L +T+G+ GLLAIL YL PA F R+L + D + + G+ L Sbjct: 324 LAAT-MPHSHNELLHATATLGIPGLLAILALYLVPAAFFLRHLGNADRGTQVASAMGLAL 382 Query: 393 VMGYVIFGLTDCFF-WGLSYTFYCIAVATLAAPLI--QSTLQQRAAT 436 G+++FGLT+ F L FY + +A A ++ + L RAA+ Sbjct: 383 CCGFMVFGLTEVMFATTLVNAFYSLIMAVCFAYVVARKDALPARAAS 429 >ref|YP_002981958.1| O-antigen polymerase [Ralstonia pickettii 12D] gb|ACS63286.1| O-antigen polymerase [Ralstonia pickettii 12D] Length = 426 Score = 170 bits (431), Expect = 5e-40, Method: Composition-based stats. Identities = 134/367 (36%), Positives = 196/367 (53%), Gaps = 17/367 (4%) Query: 79 MRRYWYVH----LGMAALFIATVIGAGFNGFNSPKVFDEPSRLAVFPLVLWMLLSLSPRQ 134 +R W+ H L MAA+ A +I A N V RL +F L L+ LL L P Q Sbjct: 64 LRAMWHEHRWLILAMAAMPTAMLISAQLNPAAPRSVPFAYGRLWLFGLALFALLQLRPTQ 123 Query: 135 VKVAQAGYVLGAIVGAVLIFVATKGGENRNNIHGAPIIVFSNIVLLLGMLSVATSTSSQS 194 ++ Q G V GA+ V ++ + G A I F N+ LL+G+ S+ + S+ Sbjct: 124 LESVQWGCVAGALASIVWAYLELRVGRPDMIGAFANTIPFGNLSLLMGLFSLISIGWSKG 183 Query: 195 ESWPWRVLRIVAALAGMYASYLSQARGSWIAIPFLGLFA-----SYLWQPNKRYVTTVVA 249 + W +LR+VA AG+YASYLSQ+RG WIAIP L L A W+ KR +V Sbjct: 184 DGWLLIILRVVAGGAGLYASYLSQSRGGWIAIPVLLLIAVATLRHVAWK--KRVTALLVF 241 Query: 250 AVLLTGALVLAWSSPMVKSRFLQVETETASFRSGTDLDTSLGTRFQLWHGSMVLFTEHPL 309 LL V SS +V++RF Q ++ ++++G +TS+G RFQLW + ++ T HPL Sbjct: 242 FALLAAVCV---SSSVVRARFDQAVSDLRAYQAG-QTNTSIGIRFQLWKAAALMLTRHPL 297 Query: 310 LGVGPGKFTAGLRELKERKIITPAAASGFYHSHNDVLFNMSTMGLLGLLAILMTYLAPAV 369 GVG G+F L+ + +IT A+ H+HN+ L+N +T+G+LG+ A+L YL PAV Sbjct: 298 AGVGRGQFEPALKAIHAEGLIT-QEATAVEHAHNEFLYNGATLGVLGIGALLAMYLVPAV 356 Query: 370 LFWRYLRHEDACLRRYAGGGVMLVMGYVIFGLTDCFF-WGLSYTFYCIAVATLAAPLIQS 428 F R +D LR G+ L +G+V+FGLT+ + FY + VA A + + Sbjct: 357 YFVRAALCDDGILRTTGAMGLTLCVGFVLFGLTEVMLIIAQTVVFYSVMVAIFTAHIHRR 416 Query: 429 TLQQRAA 435 Q AA Sbjct: 417 RQQLGAA 423