BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4429.p (300 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776945.1| small-conductance mechanosensitive channel p... 476 e-132 ref|NP_487733.1| hypothetical protein alr3693 [Nostoc sp. PCC 71... 233 3e-59 ref|YP_324095.1| mechanosensitive ion channel MscS [Anabaena var... 228 1e-57 ref|YP_002982436.1| mechanosensitive ion channel MscS [Ralstonia... 226 2e-57 ref|YP_001866279.1| MscS mechanosensitive ion channel [Nostoc pu... 226 2e-57 >ref|YP_003776945.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] gb|ADJ65037.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] Length = 276 Score = 476 bits (1224), Expect = e-132, Method: Composition-based stats. Identities = 258/275 (93%), Positives = 264/275 (96%), Gaps = 2/275 (0%) Query: 16 MPDHLLNFETLINLYLVPFGLKVIAAIVIWIVGGMFINTLSKIVRRVLTARQFETTLVNY 75 M DHLLNF+TLINLYLVPFGLKVIAAIVIWIVGGMFINTLS+IVRRVLTARQFETTL+NY Sbjct: 1 MSDHLLNFDTLINLYLVPFGLKVIAAIVIWIVGGMFINTLSRIVRRVLTARQFETTLINY 60 Query: 76 ASSAIHVILRVLLVMGILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLR 135 ASSAIHVILRVLLVMGILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLR Sbjct: 61 ASSAIHVILRVLLVMGILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLR 120 Query: 136 PFKVGDYITAAGQTGTVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDL 195 PFKVGDYITAAGQTGTVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDL Sbjct: 121 PFKVGDYITAAGQTGTVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDL 180 Query: 196 RCQIAYSVDPQQAIAKLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQ 255 RCQIAYSVDPQQAIAKLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQ Sbjct: 181 RCQIAYSVDPQQAIAKLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQ 240 Query: 256 VYNDTNRAIAQTCAEAGWPAPATYQVSV--ANPNQ 288 VYND AIA+ C + GWPAPATYQV+V A P Q Sbjct: 241 VYNDVQNAIAEVCLKEGWPAPATYQVAVPLAQPPQ 275 >ref|NP_487733.1| hypothetical protein alr3693 [Nostoc sp. PCC 7120] dbj|BAB75392.1| alr3693 [Nostoc sp. PCC 7120] Length = 286 Score = 233 bits (593), Expect = 3e-59, Method: Composition-based stats. Identities = 125/246 (50%), Positives = 172/246 (69%) Query: 31 LVPFGLKVIAAIVIWIVGGMFINTLSKIVRRVLTARQFETTLVNYASSAIHVILRVLLVM 90 L FGL+V+ AI +WI+ + IN K++RR ++ E TL+NY + + VIL+++L++ Sbjct: 14 LTQFGLRVLGAIALWIIAQLLINFGLKLLRRAFRSKHVEPTLINYIVNIVDVILKIVLIV 73 Query: 91 GILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLRPFKVGDYITAAGQTG 150 IL GI TTSFAA++ A G+A+G AWSGLL+NFAAG FLV+ RPF VGD I AAG TG Sbjct: 74 AILGFFGIETTSFAALLAAAGIAIGAAWSGLLANFAAGAFLVIFRPFAVGDTIKAAGVTG 133 Query: 151 TVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDLRCQIAYSVDPQQAIA 210 V +IGL TT I T +N++ IIGNNK+F++NI N++ N RR DL Q+ + VD Q AI Sbjct: 134 KVEEIGLFTTTINTSDNVKTIIGNNKIFADNIQNFSANPYRRVDLEAQLHHEVDHQDAIR 193 Query: 211 KLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQVYNDTNRAIAQTCAE 270 +L +RI IP+VL PAP + I EFN GT++A+R + ++ QVY DTN+AI +T E Sbjct: 194 RLKQRISQIPHVLNNPAPDVEILEFNLLGTVLAVRPYCHHEHYWQVYFDTNKAIRETFGE 253 Query: 271 AGWPAP 276 AG+P P Sbjct: 254 AGYPIP 259 >ref|YP_324095.1| mechanosensitive ion channel MscS [Anabaena variabilis ATCC 29413] gb|ABA23200.1| MscS Mechanosensitive ion channel [Anabaena variabilis ATCC 29413] Length = 286 Score = 228 bits (581), Expect = 1e-57, Method: Composition-based stats. Identities = 127/258 (49%), Positives = 177/258 (68%), Gaps = 1/258 (0%) Query: 31 LVPFGLKVIAAIVIWIVGGMFINTLSKIVRRVLTARQFETTLVNYASSAIHVILRVLLVM 90 L FGL+V+ AI +WI+ I+ K++RR + E TLVNY + ++VIL+++L++ Sbjct: 14 LTQFGLRVLGAIALWIIAQWLIDFGLKLLRRAFRNKHVEPTLVNYIVNIVNVILKIVLIV 73 Query: 91 GILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLRPFKVGDYITAAGQTG 150 IL GI TTSFAA++ A G+A+G AWSGLL+NFAAG FL++ RPF VGD I AAG TG Sbjct: 74 AILGFFGIETTSFAALLAAAGIAIGAAWSGLLANFAAGAFLIIFRPFMVGDTIQAAGVTG 133 Query: 151 TVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDLRCQIAYSVDPQQAIA 210 TV +IGL TT I T +N++ IIGNNK+F++NI N++ N RR DL+ Q+ ++VDP AI Sbjct: 134 TVEEIGLFTTTINTLDNVKTIIGNNKIFADNIQNFSANPYRRVDLQAQLHHAVDPTDAIR 193 Query: 211 KLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQVYNDTNRAIAQTCAE 270 +L ERI IP+VL PAP + I EFN G ++A+R + N+ QVY DTN+ I +T + Sbjct: 194 RLKERISQIPHVLNNPAPDVEILEFNLAGPVLAVRPYCHNENYWQVYFDTNKTIRETFGQ 253 Query: 271 AGWPAPAT-YQVSVANPN 287 AG+P P Y S +P+ Sbjct: 254 AGYPIPEQRYMFSGQSPD 271 >ref|YP_002982436.1| mechanosensitive ion channel MscS [Ralstonia pickettii 12D] gb|ACS63764.1| MscS Mechanosensitive ion channel [Ralstonia pickettii 12D] Length = 300 Score = 226 bits (577), Expect = 2e-57, Method: Composition-based stats. Identities = 135/277 (48%), Positives = 185/277 (66%), Gaps = 1/277 (0%) Query: 23 FETLINLYLVPFGLKVIAAIVIWIVGGMFINTLSKIVRRVLTARQFETTLVNYASSAIHV 82 ++ ++ Y G+K++AAIV W+VG IN ++V L+ ++ + T++ Y S I V Sbjct: 16 YQAIVAQYAADVGMKILAAIVFWVVGRWLINLAVRMVEGSLSRQKVDPTVLRYVGSIITV 75 Query: 83 ILRVLLVMGILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLRPFKVGDY 142 L VLLV+GIL GI TT+FAA+I A GVA+G+AWSGLLSNFAAG FL+VLRPFKVGD+ Sbjct: 76 TLNVLLVIGILGYFGIQTTTFAALIAAAGVAIGMAWSGLLSNFAAGAFLIVLRPFKVGDF 135 Query: 143 ITAAGQTGTVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDLRCQIAYS 202 +TA G TGT+ +IGL T I T +N+ I+GN K+F++ I NY N RR +L+ Q+A S Sbjct: 136 VTAGGVTGTIKEIGLFATTINTPDNVVTIVGNGKIFADTIQNYTSNPFRRVELKAQLAGS 195 Query: 203 VDPQQAIAKLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQVYNDTNR 262 D + AIA L E++ AIPNVL PA + I EFN G ++A+R + N+ QVY DTNR Sbjct: 196 ADHRAAIALLKEKVAAIPNVLTEPAVDVEILEFNLVGPVLAVRPYTHNDNYWQVYFDTNR 255 Query: 263 AIAQTCAEAGWPAPATYQ-VSVANPNQPAPAASVPPS 298 I + AEAG+PAP Q +++ P Q A A+V S Sbjct: 256 TIKEALAEAGFPAPTPSQALTITTPGQLAGLAAVAQS 292 >ref|YP_001866279.1| MscS mechanosensitive ion channel [Nostoc punctiforme PCC 73102] gb|ACC81336.1| MscS Mechanosensitive ion channel [Nostoc punctiforme PCC 73102] Length = 284 Score = 226 bits (577), Expect = 2e-57, Method: Composition-based stats. Identities = 130/267 (48%), Positives = 181/267 (67%), Gaps = 1/267 (0%) Query: 31 LVPFGLKVIAAIVIWIVGGMFINTLSKIVRRVLTARQFETTLVNYASSAIHVILRVLLVM 90 L FGLKV+ AI+IW++ I+ K+VRR ++Q + TL+NY + I V L+++L++ Sbjct: 14 LTLFGLKVLGAILIWLIAQRLIDFALKLVRRAFRSQQIDPTLINYLLNIIGVTLKIVLIV 73 Query: 91 GILEVCGIPTTSFAAMIGAVGVALGVAWSGLLSNFAAGIFLVVLRPFKVGDYITAAGQTG 150 IL G+ TTSFAA++ A G+A+G AW GLL+NFAAG FL+V RPFKVGD+ITA G TG Sbjct: 74 AILGFFGVETTSFAALLAAAGIAIGAAWGGLLANFAAGAFLIVFRPFKVGDFITAGGVTG 133 Query: 151 TVSDIGLVTTIITTDNNLRVIIGNNKLFSENIINYNVNATRRTDLRCQIAYSVDPQQAIA 210 TV+++GL TT I T +N+ I+ NNK+FS+NI NY+ N RR DL QI + V +AIA Sbjct: 134 TVTELGLFTTSINTPDNVLTIVANNKIFSDNIQNYSANPYRRVDLVAQIHHDVKHNEAIA 193 Query: 211 KLSERIKAIPNVLQTPAPGIVIQEFNATGTLIALRVHAPTPNFGQVYNDTNRAIAQTCAE 270 L +I IPNVLQ PAP + IQ+FN G ++A+R + ++ QVY DTN+AI +T E Sbjct: 194 LLKAKISQIPNVLQNPAPDVEIQDFNFAGPVLAVRPYCNNDHYWQVYFDTNKAIRETFNE 253 Query: 271 AGWPAPATYQVSVANPNQPAPAASVPP 297 AG+P P Y+ +V + A +PP Sbjct: 254 AGYPVP-EYRYTVNGSSAHAINNVIPP 279