BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4318.p (353 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776833.1| 2-nitropropane dioxygenase [Herbaspirillum s... 471 e-131 ref|YP_001890410.1| 2-nitropropane dioxygenase NPD [Burkholderia... 334 1e-89 ref|ZP_07047166.1| 2-nitropropane dioxygenase, NPD [Comamonas te... 328 8e-88 ref|YP_003276254.1| 2-nitropropane dioxygenase [Comamonas testos... 325 6e-87 ref|YP_001579734.1| 2-nitropropane dioxygenase NPD [Burkholderia... 319 4e-85 >ref|YP_003776833.1| 2-nitropropane dioxygenase [Herbaspirillum seropedicae SmR1] gb|ADJ64925.1| 2-nitropropane dioxygenase protein [Herbaspirillum seropedicae SmR1] Length = 356 Score = 471 bits (1212), Expect = e-131, Method: Composition-based stats. Identities = 288/348 (82%), Positives = 313/348 (89%) Query: 5 LLQRLGLAHPIIQAPMAGSSTVTMAAAASNAGALGSLALGASSVAQAREALAQLHALTDK 64 L+QRLGLAHPIIQAPMAGSSTV MAAAASNAGALGSLALGAS+ QAREALAQLHALT+K Sbjct: 7 LMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQLHALTEK 66 Query: 65 PFNVNFFCHQPAVLDAARDAAWISHLQGHLDEFGGSISLPLKEIYQSFIVDTGMQQLVLE 124 P+N+N FCHQPAVLD+ RD AW++HLQ LDEFGG I+ PLKEIYQSF DT MQQ+VLE Sbjct: 67 PYNINVFCHQPAVLDSERDRAWLAHLQPLLDEFGGRIAAPLKEIYQSFNADTQMQQVVLE 126 Query: 125 LPPAVVSFHFGLPDAGVIQSLKNAGVFLMATATNLDEARQIEAAGLDAIVAQGIEAGGHR 184 PAVVSFHFGLP A VI +L+ AG+ LMATATNLDEARQIEAAGLDAIVAQG EAGGHR Sbjct: 127 HAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGGHR 186 Query: 185 GIFDEHQDEEIGTFALVRLLAQHSRLPIIAAGGIMDGQGIAAALRLGAQAVQLGTAFLLT 244 GIFD +DE+IG+FAL+RLL QH+RLP+IAAGGIMDG GIAAAL+LGAQAVQ+GTAFLLT Sbjct: 187 GIFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGHGIAAALQLGAQAVQMGTAFLLT 246 Query: 245 RESGANAAYRNLLKSSRARHTRVTAAISGRRARGIVNRFMEEIDISPATPIPAYPAAYDA 304 RES ANA+YR LLKS RA+HTRVTAAISGR ARGIVNRFMEEID A PIPAYPAAYDA Sbjct: 247 RESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAYPAAYDA 306 Query: 305 GKQLHAMATAREVHEFGAHWAGQGAALIRDGYTVGELVHLLVQEWQAQ 352 GKQLHA+A AR+VHEFGAHWAGQGAALIRDGYTV ELVHLLVQEWQAQ Sbjct: 307 GKQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQAQ 354 >ref|YP_001890410.1| 2-nitropropane dioxygenase NPD [Burkholderia phytofirmans PsJN] gb|ACD21039.1| 2-nitropropane dioxygenase NPD [Burkholderia phytofirmans PsJN] Length = 367 Score = 334 bits (857), Expect = 1e-89, Method: Composition-based stats. Identities = 201/349 (57%), Positives = 239/349 (68%) Query: 5 LLQRLGLAHPIIQAPMAGSSTVTMAAAASNAGALGSLALGASSVAQAREALAQLHALTDK 64 LQ +G HPIIQAPMAG ST +AAA SNAGALGS+A+GAS+ QARE + ALT K Sbjct: 7 FLQAIGAQHPIIQAPMAGISTPALAAAVSNAGALGSIAVGASTAQQARELIVATRALTSK 66 Query: 65 PFNVNFFCHQPAVLDAARDAAWISHLQGHLDEFGGSISLPLKEIYQSFIVDTGMQQLVLE 124 PFNVN FCH+PA D R+A W++HLQ EFG + LKEIY SF+VD + Q+++E Sbjct: 67 PFNVNVFCHRPAYSDPLREATWLAHLQPLFAEFGARTPVALKEIYTSFVVDEALLQVLIE 126 Query: 125 LPPAVVSFHFGLPDAGVIQSLKNAGVFLMATATNLDEARQIEAAGLDAIVAQGIEAGGHR 184 PAVVSFHFGLP I L AG+ L AT EARQIE AGLDAIVAQG EAGGHR Sbjct: 127 ERPAVVSFHFGLPSREWINELHRAGILLFGCATTPHEARQIEQAGLDAIVAQGAEAGGHR 186 Query: 185 GIFDEHQDEEIGTFALVRLLAQHSRLPIIAAGGIMDGQGIAAALRLGAQAVQLGTAFLLT 244 G+F+ D IGT ALVR++A+ S LP+IAAGGIMDGQ +A AL LGA AVQ+GTAF+L Sbjct: 187 GVFNPDDDAMIGTLALVRMIARQSTLPVIAAGGIMDGQSVAGALLLGASAVQMGTAFVLC 246 Query: 245 RESGANAAYRNLLKSSRARHTRVTAAISGRRARGIVNRFMEEIDISPATPIPAYPAAYDA 304 ESGA+AAYR L + RA T VTAAISGR ARG+ NR ++D A P+P YP AYDA Sbjct: 247 PESGADAAYRTALTTQRAYRTAVTAAISGRPARGLSNRLYIDVDSPDAPPLPDYPIAYDA 306 Query: 305 GKQLHAMATAREVHEFGAHWAGQGAALIRDGYTVGELVHLLVQEWQAQG 353 K L+A A A+ EF WAGQGA L R + L+ + +AQG Sbjct: 307 AKALNAAARAKGNTEFAVQWAGQGAPLARALPAAQLVATLMSELAEAQG 355 >ref|ZP_07047166.1| 2-nitropropane dioxygenase, NPD [Comamonas testosteroni S44] gb|EFI59197.1| 2-nitropropane dioxygenase, NPD [Comamonas testosteroni S44] Length = 355 Score = 328 bits (841), Expect = 8e-88, Method: Composition-based stats. Identities = 195/343 (56%), Positives = 244/343 (71%), Gaps = 2/343 (0%) Query: 9 LGLAHPIIQAPMAGSSTVTMAAAASNAGALGSLALGASSVAQAREALAQLHALTDKPFNV 68 LGL HPIIQAPMAG+ST +AAA SNA ALGS+ +GA + QAR+ + Q ALTD+PFNV Sbjct: 11 LGLQHPIIQAPMAGTSTPQLAAAVSNAYALGSIGIGAYGLEQARQHIEQTRALTDRPFNV 70 Query: 69 NFFCHQPAVLDAARDAAWISHLQGHLDEFGGSISLPLKEIYQSFIVDTGMQQLVLELPPA 128 N FCH+ V DA R+A W+ +L + +G S L+ IY+S + + +Q ++LEL PA Sbjct: 71 NLFCHRAPVADAEREAQWLRYLAPEFERYGASAPPSLRCIYESALGNERLQAMLLELRPA 130 Query: 129 VVSFHFGLPDAGVIQSLKNAGVFLMATATNLDEARQIEAAGLDAIVAQGIEAGGHRGIFD 188 VVSFHFGLP+ G + +L AG+ +A AT+LDEARQIE AG+D IVAQG+EAGGHRG F Sbjct: 131 VVSFHFGLPEQGFVDALCAAGITTLACATSLDEARQIEQAGVDVIVAQGMEAGGHRGAFV 190 Query: 189 EHQDEEIGTFALVRLLAQHSRLPIIAAGGIMDGQGIAAALRLGAQAVQLGTAFLLTRESG 248 QD +GT ALVRLLA+ SRLP++AAGGIMDG GIAAAL+LGA AVQ+GTAF+L ES Sbjct: 191 PEQDRLMGTLALVRLLARESRLPVVAAGGIMDGAGIAAALQLGACAVQMGTAFILCPESA 250 Query: 249 ANAAYRNLLKSSRARHTRVTAAISGRRARGIVNRFMEEIDISPATPIPAYPAAYDAGKQL 308 A+ AYR L+ A+HT +TAAISGR ARG+ NR + ++ A +PAYP YDAGK L Sbjct: 251 ASQAYRAELQGDSAQHTAITAAISGRPARGMFNR-LHQLGRDYAGALPAYPMVYDAGKAL 309 Query: 309 HAMATAREVHEFGAHWAGQGAALIRDGYTVGELVHLLVQEWQA 351 H A+A+ ++ AHWAGQGA L R ELV L QE A Sbjct: 310 HQAASAQGSSDYAAHWAGQGAPLAR-AMPAAELVRTLAQELDA 351 >ref|YP_003276254.1| 2-nitropropane dioxygenase [Comamonas testosteroni CNB-2] gb|ACY30958.1| 2-nitropropane dioxygenase, NPD [Comamonas testosteroni CNB-2] Length = 348 Score = 325 bits (834), Expect = 6e-87, Method: Composition-based stats. Identities = 193/342 (56%), Positives = 246/342 (71%), Gaps = 2/342 (0%) Query: 9 LGLAHPIIQAPMAGSSTVTMAAAASNAGALGSLALGASSVAQAREALAQLHALTDKPFNV 68 LGL HPIIQAPMAG+ST +AAA SNA ALGS+ +GA + QAR+ + Q ALT++PFNV Sbjct: 4 LGLQHPIIQAPMAGTSTPQLAAAVSNAYALGSVGIGAYGLEQARQHIEQTRALTNRPFNV 63 Query: 69 NFFCHQPAVLDAARDAAWISHLQGHLDEFGGSISLPLKEIYQSFIVDTGMQQLVLELPPA 128 N FCH+ V DA R+A W+ +L + +G S+ L+ IY+S + + +Q ++LEL PA Sbjct: 64 NLFCHRAPVADAEREAQWLRYLAPEFERYGASVPSGLRCIYESALGNERLQAMLLELRPA 123 Query: 129 VVSFHFGLPDAGVIQSLKNAGVFLMATATNLDEARQIEAAGLDAIVAQGIEAGGHRGIFD 188 VVSFHFGLP+ G + +L+ AG+ +A AT+LDEARQIE AG+D IVAQG+EAGGHRG F Sbjct: 124 VVSFHFGLPEQGFVDALRAAGITTLACATSLDEARQIEQAGVDVIVAQGMEAGGHRGAFV 183 Query: 189 EHQDEEIGTFALVRLLAQHSRLPIIAAGGIMDGQGIAAALRLGAQAVQLGTAFLLTRESG 248 QD +GT ALVRLLA+ SRLP++AAGGIMDG GIAAAL+LGA AVQ+GTAF+L ES Sbjct: 184 PEQDTLMGTLALVRLLARESRLPVVAAGGIMDGAGIAAALQLGACAVQMGTAFILCPESA 243 Query: 249 ANAAYRNLLKSSRARHTRVTAAISGRRARGIVNRFMEEIDISPATPIPAYPAAYDAGKQL 308 A+ AYR L+ A+HT +TAAISGR ARG+ NR + ++ A +PAYP YDAGK L Sbjct: 244 ASQAYRAELQGDSAQHTAITAAISGRPARGMFNR-LHQLGRDYAGALPAYPMVYDAGKAL 302 Query: 309 HAMATAREVHEFGAHWAGQGAALIRDGYTVGELVHLLVQEWQ 350 H A+A+ ++ AHWAGQGA L R ELV L QE + Sbjct: 303 HQAASAQGSSDYAAHWAGQGAPLAR-AMPAAELVRTLAQELE 343 >ref|YP_001579734.1| 2-nitropropane dioxygenase NPD [Burkholderia multivorans ATCC 17616] ref|YP_001946151.1| 2-nitropropane dioxygenase [Burkholderia multivorans ATCC 17616] gb|ABX15237.1| 2-nitropropane dioxygenase NPD [Burkholderia multivorans ATCC 17616] dbj|BAG43615.1| 2-nitropropane dioxygenase [Burkholderia multivorans ATCC 17616] Length = 366 Score = 319 bits (817), Expect = 4e-85, Method: Composition-based stats. Identities = 201/349 (57%), Positives = 241/349 (69%), Gaps = 2/349 (0%) Query: 4 ALLQRLGLAHPIIQAPMAGSSTVTMAAAASNAGALGSLALGASSVAQAREALAQLHALTD 63 ALL++LG+ PIIQAPMAG ST +AAA SNAG LGSL +GA++ AR+ + ALTD Sbjct: 9 ALLRQLGIRVPIIQAPMAGVSTPALAAAVSNAGGLGSLGVGATNADGARKMIRDTRALTD 68 Query: 64 KPFNVNFFCHQPAVLDAARDAAWISHLQGHLDEFGGSISLPLKEIYQSFIVDTGMQQLVL 123 +PFN+N FCHQPA DAA + AW+ L E+G + L EIY SF+ D+ MQ +++ Sbjct: 69 RPFNINVFCHQPARADAAIERAWLDWLAPAFREYGATPPASLSEIYTSFVADSAMQAMLV 128 Query: 124 ELPPAVVSFHFGLPDAGVIQSLKNAGVFLMATATNLDEARQIEAAGLDAIVAQGIEAGGH 183 E PAVVSFHFGLP A VI +LK AGV L A+ATNLDEARQI AG+DAIVAQG+EAGGH Sbjct: 129 EEKPAVVSFHFGLPSADVIAALKRAGVTLFASATNLDEARQIADAGIDAIVAQGVEAGGH 188 Query: 184 RGIFD-EHQDEEIGTFALVRLLAQHSRLPIIAAGGIMDGQGIAAALRLGAQAVQLGTAFL 242 RG+FD +D+ +GTFAL RLL + LP+IAAGGIMDG+GIAA L LGAQA QLGTAF+ Sbjct: 189 RGVFDPAARDDRLGTFALTRLLVRECALPVIAAGGIMDGEGIAAVLALGAQAAQLGTAFV 248 Query: 243 LTRESGANAAYRNLLKSSRARHTRVTAAISGRRARGIVNRFMEEIDISPATPIPAYPAAY 302 E+ + YR + S RHT TAAISGR ARGI NRF D A PAYP AY Sbjct: 249 ACPETSIDDGYRRAILSDATRHTTFTAAISGRVARGIANRFTALGDDPRAPDTPAYPIAY 308 Query: 303 DAGKQLHAMATAREVHEFGAHWAGQGAALIRDGYTVGELVHLLVQEWQA 351 DAGK LHA A A+ +GA WAGQ A L+R ELV L +E +A Sbjct: 309 DAGKALHAAAKAKGEFGYGAQWAGQAAPLVR-ALPAAELVATLERETRA 356