BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4316.p (346 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776832.1| thymidylate synthetase [Herbaspirillum serop... 654 0.0 ref|YP_001352883.1| thymidylate synthase [Janthinobacterium sp. ... 552 e-155 ref|YP_001099362.1| thymidylate synthase [Herminiimonas arsenico... 543 e-152 ref|ZP_02378966.1| Thymidylate synthase [Burkholderia ubonensis Bu] 530 e-148 ref|YP_368440.1| thymidylate synthase [Burkholderia sp. 383] >gi... 528 e-148 >ref|YP_003776832.1| thymidylate synthetase [Herbaspirillum seropedicae SmR1] gb|ADJ64924.1| thymidylate synthetase protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 654 bits (1688), Expect = 0.0, Method: Composition-based stats. Identities = 306/323 (94%), Positives = 313/323 (96%) Query: 24 MKQYQDLIQDVLTNGSWQDNRTGIRTLSIPGAMMRFDLQKGFPAVTTKRLAFKSVVGELC 83 MKQYQDLIQDVL NGSWQDNRTGIRTLSIPGAMMRFDLQKGFPAVTTKRLAFKSVVGELC Sbjct: 1 MKQYQDLIQDVLENGSWQDNRTGIRTLSIPGAMMRFDLQKGFPAVTTKRLAFKSVVGELC 60 Query: 84 GFLRATRSAADFRALGCKVWDQNANENADWLANPFRTGTDDLGPVYGVQWREWPAYKLID 143 GFLRATRSAADFRALGCKVWDQNANENADWLANPFR+G DDLGPVYGVQWR+WPAYKLID Sbjct: 61 GFLRATRSAADFRALGCKVWDQNANENADWLANPFRSGADDLGPVYGVQWRQWPAYKLID 120 Query: 144 AAHTAQIDAARAKGFAIVSSLQDNGVEKVLLYKAVDQLRECLDTIVKNPGSRRILFHGWN 203 AAHT QI+AAR+KGFAIVSSLQD GVEKVLLYKAVDQLRECLDTIV NPGSRRILFHGWN Sbjct: 121 AAHTTQIEAARSKGFAIVSSLQDEGVEKVLLYKAVDQLRECLDTIVNNPGSRRILFHGWN 180 Query: 204 CAVLDEIALPACHLLYQFLPNQSTGEISLCLYVRSNDIGLGTPFNLAEGAALLHLVARLT 263 CAVLDE+ALPACHLLYQFLPNQSTGEISLCLYVRSNDIGLGTPFNLAEGAALLHLVARLT Sbjct: 181 CAVLDEVALPACHLLYQFLPNQSTGEISLCLYVRSNDIGLGTPFNLAEGAALLHLVARLT 240 Query: 264 GYKPRWFSYFVGDAHIYENHIDMVKEQLTREPYPLPQLAIAERVPEFARTREYAPQWLEL 323 GYKPRWFSYFVGDAHIYENHIDMVKEQLTR PYPLPQL IA+RVPEFART Y PQWLEL Sbjct: 241 GYKPRWFSYFVGDAHIYENHIDMVKEQLTRAPYPLPQLEIADRVPEFARTGRYEPQWLEL 300 Query: 324 VEPTDFSLVNYQHHAPLTAPMAV 346 VEP+DFSLVNYQHHAPLTAPMAV Sbjct: 301 VEPSDFSLVNYQHHAPLTAPMAV 323 >ref|YP_001352883.1| thymidylate synthase [Janthinobacterium sp. Marseille] gb|ABR88728.1| thymidylate synthase [Janthinobacterium sp. Marseille] Length = 324 Score = 552 bits (1423), Expect = e-155, Method: Composition-based stats. Identities = 247/324 (76%), Positives = 288/324 (88%), Gaps = 1/324 (0%) Query: 24 MKQYQDLIQDVLTNGSWQDNRTGIRTLSIPGAMMRFDLQK-GFPAVTTKRLAFKSVVGEL 82 MKQY DL++ VL G+WQ+NRTGIRT+SIPGAM+RFDLQK GFPA+TTKRLAFKSV+GEL Sbjct: 1 MKQYLDLVRTVLDTGTWQENRTGIRTISIPGAMLRFDLQKDGFPAITTKRLAFKSVIGEL 60 Query: 83 CGFLRATRSAADFRALGCKVWDQNANENADWLANPFRTGTDDLGPVYGVQWREWPAYKLI 142 GFLRATRSAA+FR LGCKVWDQNANEN +WL NP+RTG DDLGPVYGVQWR+WPAYKL+ Sbjct: 61 IGFLRATRSAAEFRELGCKVWDQNANENTEWLNNPYRTGPDDLGPVYGVQWRQWPAYKLL 120 Query: 143 DAAHTAQIDAARAKGFAIVSSLQDNGVEKVLLYKAVDQLRECLDTIVKNPGSRRILFHGW 202 DA AQID A+ +GF +VS++ D G +KVLLYKA+DQLR+CLDTI+KNPGSRRILFHGW Sbjct: 121 DADQQAQIDDAQKRGFRVVSTVNDEGKDKVLLYKAIDQLRDCLDTIIKNPGSRRILFHGW 180 Query: 203 NCAVLDEIALPACHLLYQFLPNQSTGEISLCLYVRSNDIGLGTPFNLAEGAALLHLVARL 262 NCA LD IALPACHLLYQF+PN +T EISLCLY+RSNDIGLGTPFNLAE AALLH+V RL Sbjct: 181 NCADLDAIALPACHLLYQFIPNATTKEISLCLYIRSNDIGLGTPFNLAEAAALLHVVGRL 240 Query: 263 TGYKPRWFSYFVGDAHIYENHIDMVKEQLTREPYPLPQLAIAERVPEFARTREYAPQWLE 322 TGY P+WF+YF+GDAHIYENH+DM+ EQ+TR P+P PQL +++R+P+FA T +Y P+WLE Sbjct: 241 TGYTPKWFTYFIGDAHIYENHLDMLNEQMTRAPHPAPQLVLSDRIPDFAVTGKYEPEWLE 300 Query: 323 LVEPTDFSLVNYQHHAPLTAPMAV 346 +EP DFSL NYQHHAPLTAPMAV Sbjct: 301 KLEPGDFSLENYQHHAPLTAPMAV 324 >ref|YP_001099362.1| thymidylate synthase [Herminiimonas arsenicoxydans] emb|CAL61235.1| thymidylate synthase (TS) (TSase) [Herminiimonas arsenicoxydans] Length = 324 Score = 543 bits (1400), Expect = e-152, Method: Composition-based stats. Identities = 240/324 (74%), Positives = 288/324 (88%), Gaps = 1/324 (0%) Query: 24 MKQYQDLIQDVLTNGSWQDNRTGIRTLSIPGAMMRFDLQK-GFPAVTTKRLAFKSVVGEL 82 MKQY DL++ VL +G+WQ+NRTGIRT+SIPGAM+RFDLQK GFPA+TTKRLAFKSV+GEL Sbjct: 1 MKQYLDLVRTVLDSGTWQENRTGIRTISIPGAMLRFDLQKDGFPAITTKRLAFKSVIGEL 60 Query: 83 CGFLRATRSAADFRALGCKVWDQNANENADWLANPFRTGTDDLGPVYGVQWREWPAYKLI 142 GFLRA+RSAA+FRALGCKVWDQNANENA+WL NP+R G DDLGPVYGVQWR+WPAYKL+ Sbjct: 61 IGFLRASRSAAEFRALGCKVWDQNANENAEWLNNPYRLGEDDLGPVYGVQWRQWPAYKLL 120 Query: 143 DAAHTAQIDAARAKGFAIVSSLQDNGVEKVLLYKAVDQLRECLDTIVKNPGSRRILFHGW 202 DA + QI A+ +GF ++S++ D G++KVL+YKA+DQLR+CLDTI+ NP SRRILFHGW Sbjct: 121 DADNKLQIQDAQKRGFRLISAVNDEGIDKVLMYKAIDQLRDCLDTIINNPASRRILFHGW 180 Query: 203 NCAVLDEIALPACHLLYQFLPNQSTGEISLCLYVRSNDIGLGTPFNLAEGAALLHLVARL 262 NCA LD IALPACHLLYQFLPNQ+T EISLCLY+RSNDIGLGTPFNLAE AALLH+V RL Sbjct: 181 NCAELDAIALPACHLLYQFLPNQTTKEISLCLYIRSNDIGLGTPFNLAEAAALLHVVGRL 240 Query: 263 TGYKPRWFSYFVGDAHIYENHIDMVKEQLTREPYPLPQLAIAERVPEFARTREYAPQWLE 322 TGY P+WF+YF+GDAHIYENH+DM+ EQ+TREPY P+L +++R+P++ T +Y P+WLE Sbjct: 241 TGYTPKWFTYFIGDAHIYENHLDMLNEQMTREPYAAPRLVLSDRIPDYRVTGKYEPEWLE 300 Query: 323 LVEPTDFSLVNYQHHAPLTAPMAV 346 +EP DFSL NYQHHAPL+APMAV Sbjct: 301 KLEPGDFSLENYQHHAPLSAPMAV 324 >ref|ZP_02378966.1| Thymidylate synthase [Burkholderia ubonensis Bu] Length = 323 Score = 530 bits (1364), Expect = e-148, Method: Composition-based stats. Identities = 242/323 (74%), Positives = 279/323 (86%) Query: 24 MKQYQDLIQDVLTNGSWQDNRTGIRTLSIPGAMMRFDLQKGFPAVTTKRLAFKSVVGELC 83 MKQY DL++ +L G+WQ+NRTGIRT+S+PGAM+RFDLQ+GFPAVTTK+LAFKS +GEL Sbjct: 1 MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELV 60 Query: 84 GFLRATRSAADFRALGCKVWDQNANENADWLANPFRTGTDDLGPVYGVQWREWPAYKLID 143 GFLRA+RSAADFRALGCKVWD NANENA WLANP R G DDLG VYGVQWR WP YK++D Sbjct: 61 GFLRASRSAADFRALGCKVWDANANENAQWLANPHRQGVDDLGDVYGVQWRRWPGYKVLD 120 Query: 144 AAHTAQIDAARAKGFAIVSSLQDNGVEKVLLYKAVDQLRECLDTIVKNPGSRRILFHGWN 203 A AQI A +GF IV+ ++G KVLLYKA+DQLRECLDTI+++PGSRRILFHGWN Sbjct: 121 AGADAQIADATGRGFRIVARFDEDGAPKVLLYKAIDQLRECLDTIMQDPGSRRILFHGWN 180 Query: 204 CAVLDEIALPACHLLYQFLPNQSTGEISLCLYVRSNDIGLGTPFNLAEGAALLHLVARLT 263 AVLDEIALPACHLLYQFLPN + EISLCLY+RSND+GLGTPFNL EGAALL LV RLT Sbjct: 181 PAVLDEIALPACHLLYQFLPNVAKREISLCLYIRSNDVGLGTPFNLTEGAALLSLVGRLT 240 Query: 264 GYKPRWFSYFVGDAHIYENHIDMVKEQLTREPYPLPQLAIAERVPEFARTREYAPQWLEL 323 GY PRWF+YFVGDAHIYEN +DM+++QL REPY P+LAIA+RVPE+A+T YAP+WLE Sbjct: 241 GYTPRWFTYFVGDAHIYENQLDMLQQQLAREPYESPRLAIADRVPEYAQTGVYAPEWLEK 300 Query: 324 VEPTDFSLVNYQHHAPLTAPMAV 346 VEP+DFSLV Y+HH PLTAPMAV Sbjct: 301 VEPSDFSLVGYRHHEPLTAPMAV 323 >ref|YP_368440.1| thymidylate synthase [Burkholderia sp. 383] gb|ABB07796.1| thymidylate synthase [Burkholderia sp. 383] Length = 323 Score = 528 bits (1360), Expect = e-148, Method: Composition-based stats. Identities = 238/323 (73%), Positives = 278/323 (86%) Query: 24 MKQYQDLIQDVLTNGSWQDNRTGIRTLSIPGAMMRFDLQKGFPAVTTKRLAFKSVVGELC 83 MKQY DL++ +L G+WQ+NRTGIRT+S+PGAM+RFDLQ+GFPAVTTK+LAFKS +GEL Sbjct: 1 MKQYLDLVRTILDTGTWQENRTGIRTISMPGAMLRFDLQQGFPAVTTKKLAFKSAIGELV 60 Query: 84 GFLRATRSAADFRALGCKVWDQNANENADWLANPFRTGTDDLGPVYGVQWREWPAYKLID 143 GFLRA+RSAADFRALGCKVWD NANENA WLANP+R G DDLG VYGVQWR+WP YK++D Sbjct: 61 GFLRASRSAADFRALGCKVWDANANENAQWLANPYRQGVDDLGDVYGVQWRQWPGYKVLD 120 Query: 144 AAHTAQIDAARAKGFAIVSSLQDNGVEKVLLYKAVDQLRECLDTIVKNPGSRRILFHGWN 203 A AQID A +GF IV+ ++G KVLLYKA+DQLR+CLDTI++NP RRILFH WN Sbjct: 121 AGADAQIDDATRRGFRIVTRFDEDGAPKVLLYKAIDQLRQCLDTIMENPADRRILFHAWN 180 Query: 204 CAVLDEIALPACHLLYQFLPNQSTGEISLCLYVRSNDIGLGTPFNLAEGAALLHLVARLT 263 AVLD+IALPACHLLYQFLPN + EISLCLY+RSND+GLGTPFNL EGAALL LV RLT Sbjct: 181 PAVLDQIALPACHLLYQFLPNATKREISLCLYIRSNDVGLGTPFNLTEGAALLELVGRLT 240 Query: 264 GYKPRWFSYFVGDAHIYENHIDMVKEQLTREPYPLPQLAIAERVPEFARTREYAPQWLEL 323 GY PRWF+YF+GDAHIYEN +DM++ QLTREPY P+L IAERVPE+A+T YAP+WLE Sbjct: 241 GYTPRWFTYFIGDAHIYENQLDMLQRQLTREPYDSPRLEIAERVPEYAKTGVYAPEWLEQ 300 Query: 324 VEPTDFSLVNYQHHAPLTAPMAV 346 +EP+DFSLV Y+HH PLTAPMAV Sbjct: 301 IEPSDFSLVGYRHHEPLTAPMAV 323