BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4302.p (567 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776820.1| 2-isopropylmalate synthase [Herbaspirillum s... 1140 0.0 ref|YP_004752177.1| 2-isopropylmalate synthase [Collimonas fungi... 954 0.0 ref|YP_001352888.1| 2-isopropylmalate synthase [Janthinobacteriu... 877 0.0 emb|CAL61215.2| 2-isopropylmalate synthase (Alpha-isopropylmalat... 863 0.0 ref|YP_728252.1| 2-isopropylmalate synthase [Ralstonia eutropha ... 833 0.0 >ref|YP_003776820.1| 2-isopropylmalate synthase [Herbaspirillum seropedicae SmR1] gb|ADJ64912.1| 2-isopropylmalate synthase protein [Herbaspirillum seropedicae SmR1] Length = 567 Score = 1140 bits (2948), Expect = 0.0, Method: Composition-based stats. Identities = 559/567 (98%), Positives = 567/567 (100%) Query: 1 MMLSNPAAKYRPFPAIDLPDRTWPSKVIDTPPIWMSTDLRDGNQALIEPMNAERKLRFFE 60 MMLSNPAAKYRPFPAIDLPDRTWPSKVI+TPPIWMSTDLRDGNQALIEPMNAERKLRFFE Sbjct: 1 MMLSNPAAKYRPFPAIDLPDRTWPSKVINTPPIWMSTDLRDGNQALIEPMNAERKLRFFE 60 Query: 61 LLLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSREELIRRTVESLEG 120 LLLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSREELIRRT+ESLEG Sbjct: 61 LLLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSREELIRRTIESLEG 120 Query: 121 AKKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESF 180 AKKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESF Sbjct: 121 AKKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESF 180 Query: 181 STTELDFSKDICDAVCETWGATPERKIILNLPSTVECATPNVYADQIEWMCRNLKDRAST 240 STTELDFSK+ICDAVCETWGA+PERK+ILNLPSTVECATPNVYADQIEWMCRNLKDRAST Sbjct: 181 STTELDFSKEICDAVCETWGASPERKVILNLPSTVECATPNVYADQIEWMCRNLKDRAST 240 Query: 241 IISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGL 300 IISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGL Sbjct: 241 IISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGL 300 Query: 301 DFSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPY 360 DFSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPY Sbjct: 301 DFSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPY 360 Query: 361 LPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGK 420 LPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGK Sbjct: 361 LPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGK 420 Query: 421 EIAASDIHAIFQREYLERVEPYVYRAHRMSEDTAKAESINIEVDIVRNGQPVTVRGSGNG 480 EIAASDIHAIFQREYLERVEPYVYRAHRMSED++KAESINIEVDIVRNGQPVTVRGSGNG Sbjct: 421 EIAASDIHAIFQREYLERVEPYVYRAHRMSEDSSKAESINIEVDIVRNGQPVTVRGSGNG 480 Query: 481 PIDAFVHALGLDIKLMDFHEHAIGAGADAKAASYIELRLNEAPTGFGVGIDANIVTASFK 540 PIDAFVHALGLDIKLMDFHEHAIGAGADAKAASYIELRLNEAPTGFGVGIDANIVTASFK Sbjct: 481 PIDAFVHALGLDIKLMDFHEHAIGAGADAKAASYIELRLNEAPTGFGVGIDANIVTASFK 540 Query: 541 AVLSAVNRQIAISESANQAGSAQAKAA 567 AVLSAVNRQIAISESANQAGS+QAKAA Sbjct: 541 AVLSAVNRQIAISESANQAGSSQAKAA 567 >ref|YP_004752177.1| 2-isopropylmalate synthase [Collimonas fungivorans Ter331] gb|AEK61354.1| 2-isopropylmalate synthase [Collimonas fungivorans Ter331] Length = 568 Score = 954 bits (2467), Expect = 0.0, Method: Composition-based stats. Identities = 460/568 (80%), Positives = 515/568 (90%), Gaps = 1/568 (0%) Query: 1 MMLSNPAAKYRPFPAIDLPDRTWPSKVIDTPPIWMSTDLRDGNQALIEPMNAERKLRFFE 60 MML NPA KYRPFP I L DRTWP++VI PPIWMSTDLRDGNQALIEPM+A+RKLRFF+ Sbjct: 1 MMLHNPAVKYRPFPPIPLSDRTWPNQVISAPPIWMSTDLRDGNQALIEPMDADRKLRFFK 60 Query: 61 LLLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSREELIRRTVESLEG 120 LL+K GLKEIEVGFPS SQTDFDFVRKLI EN IPDDVTIIVLTQSRE+LIRRTV+SL+ Sbjct: 61 LLVKIGLKEIEVGFPSGSQTDFDFVRKLIEENHIPDDVTIIVLTQSREDLIRRTVDSLQN 120 Query: 121 AKKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESF 180 AK+AIVHLYN +APA+R+IVF+ SR+E+K IAV+GTRL+KELTDARP T+WR+EYSPE+F Sbjct: 121 AKQAIVHLYNPIAPAWRRIVFDKSRDEVKEIAVSGTRLIKELTDARPETQWRYEYSPETF 180 Query: 181 STTELDFSKDICDAVCETWGATPERKIILNLPSTVECATPNVYADQIEWMCRNLKDRAST 240 S ELDFSK++CDAV E W A+P RK+ILNLP+TVEC+TPNV+ADQIEWM RNL R +T Sbjct: 181 SMAELDFSKEVCDAVVEAWHASPTRKVILNLPTTVECSTPNVFADQIEWMHRNLARRDAT 240 Query: 241 IISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGL 300 IISVHPHNDRGT VA+AELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGL Sbjct: 241 IISVHPHNDRGTGVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGL 300 Query: 301 DFSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPY 360 DFSDID VRQ VE+CNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFA Q+ DAIWE+PY Sbjct: 301 DFSDIDEVRQCVEDCNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAAQKADAIWEIPY 360 Query: 361 LPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGK 420 LPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLA+PRRLQIEFSRAIQ+ ADATGK Sbjct: 361 LPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLAMPRRLQIEFSRAIQKAADATGK 420 Query: 421 EIAASDIHAIFQREYLERVEPYVYRAHRMSEDTAKAESINIEVDIVRNGQPVTVRGSGNG 480 EIAASDI+ IF++EYL++ PYVYR HRMSED+++A+ + IE+DIVR+GQ T G GNG Sbjct: 421 EIAASDIYNIFKQEYLDKQTPYVYRGHRMSEDSSQAQPVRIEIDIVRDGQAHTSSGHGNG 480 Query: 481 PIDAFVHALGLDIKLMDFHEHAIGAGADAKAASYIELRLNEAPTGFGVGIDANIVTASFK 540 PIDAFV+ALGLDIKLMDFHEHAI AGADA+AASYIELRLN+APTGFGVGIDAN +TASFK Sbjct: 481 PIDAFVNALGLDIKLMDFHEHAISAGADAQAASYIELRLNDAPTGFGVGIDANTLTASFK 540 Query: 541 AVLSAVNRQIAIS-ESANQAGSAQAKAA 567 A+LSAVNRQI I+ ++ANQ A AA Sbjct: 541 AILSAVNRQIEIAGQAANQEIGGNAAAA 568 >ref|YP_001352888.1| 2-isopropylmalate synthase [Janthinobacterium sp. Marseille] gb|ABR88384.1| 2-isopropylmalate synthase [Janthinobacterium sp. Marseille] Length = 552 Score = 877 bits (2265), Expect = 0.0, Method: Composition-based stats. Identities = 422/553 (76%), Positives = 487/553 (88%), Gaps = 2/553 (0%) Query: 1 MMLSNPAAKYRPFPAIDLPDRTWPSKVIDTPPIWMSTDLRDGNQALIEPMNAERKLRFFE 60 MML+NPAAKYR FP + L DRTWP++VI TPPIWMSTDLRDGNQALIEPMN E+KLRFF+ Sbjct: 1 MMLTNPAAKYRAFPPVQLKDRTWPNRVISTPPIWMSTDLRDGNQALIEPMNHEKKLRFFD 60 Query: 61 LLLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSREELIRRTVESLEG 120 +LLK GLKEIEV FPSASQTDFDFVRKLI E RIPDDVTI VLTQ+RE+LIRRTV+S+ G Sbjct: 61 MLLKIGLKEIEVAFPSASQTDFDFVRKLIDEKRIPDDVTIEVLTQAREDLIRRTVDSVVG 120 Query: 121 AKKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESF 180 +++AIVH+YN+ APAFR+IVFNMS+EE+K +AV GT+L++ELTDA P TEW F+YSPE+F Sbjct: 121 SRRAIVHMYNATAPAFRRIVFNMSKEEVKALAVAGTKLIRELTDAHPETEWVFQYSPETF 180 Query: 181 STTELDFSKDICDAVCETWGATPERKIILNLPSTVECATPNVYADQIEWMCRNLKDRAST 240 S TELDF+K++CDAV E W TPERK+I+NLP TVE +TPNVYADQ+EWM RNL R S Sbjct: 181 SMTELDFAKEVCDAVSEAWQPTPERKMIINLPGTVEASTPNVYADQVEWMHRNLARRDSI 240 Query: 241 IISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGL 300 I+SVHPHNDRG AVA+AELAVMAGADR+EGCLFGNGERTGNVDLVTLALNLY+QG+NPGL Sbjct: 241 ILSVHPHNDRGCAVAAAELAVMAGADRIEGCLFGNGERTGNVDLVTLALNLYSQGINPGL 300 Query: 301 DFSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPY 360 DFSDID +RQ VEECNQIPVHPRHPYVGDLV+TAFSGSHQDAIKKGFAKQ+PD IWEVPY Sbjct: 301 DFSDIDEIRQCVEECNQIPVHPRHPYVGDLVYTAFSGSHQDAIKKGFAKQKPDEIWEVPY 360 Query: 361 LPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGK 420 +P+DPADLGRSYDAVIRVNSQSGKGGM YLLEQEYGL LPRRLQIEFSRA+QR D TG Sbjct: 361 MPVDPADLGRSYDAVIRVNSQSGKGGMTYLLEQEYGLVLPRRLQIEFSRAVQRITDETGG 420 Query: 421 EIAASDIHAIFQREYLERVEPYVYRAHRMSEDTAKAESINIEVDIVRNGQPVTVRGSGNG 480 E+AA+DIH IF EYL V PY YR H + D +K + I+V++ ++G+PV+++G GNG Sbjct: 421 EVAANDIHTIFNSEYLNAVTPYHYRNHHI--DDSKDGEVQIKVELEKDGKPVSMQGVGNG 478 Query: 481 PIDAFVHALGLDIKLMDFHEHAIGAGADAKAASYIELRLNEAPTGFGVGIDANIVTASFK 540 PIDAF++ALGL+IKLMDFHEHAIGAGA A AASY+ELR+N T FGVGID NIVTASF+ Sbjct: 479 PIDAFINALGLNIKLMDFHEHAIGAGASANAASYVELRVNNGVTRFGVGIDGNIVTASFR 538 Query: 541 AVLSAVNRQIAIS 553 AVLSAVNR IA++ Sbjct: 539 AVLSAVNRDIALA 551 >emb|CAL61215.2| 2-isopropylmalate synthase (Alpha-isopropylmalate synthase) (Alpha-IPM synthetase) [Herminiimonas arsenicoxydans] Length = 553 Score = 863 bits (2230), Expect = 0.0, Method: Composition-based stats. Identities = 418/553 (75%), Positives = 481/553 (86%), Gaps = 1/553 (0%) Query: 1 MMLSNPAAKYRPFPAIDLPDRTWPSKVIDTPPIWMSTDLRDGNQALIEPMNAERKLRFFE 60 MMLSNP KYR FP + L DR WP++VI PIWMSTDLRDGNQ+LIEPMN ++KLRFF+ Sbjct: 1 MMLSNPEQKYRAFPPVRLTDRNWPNQVISKAPIWMSTDLRDGNQSLIEPMNHDKKLRFFD 60 Query: 61 LLLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSREELIRRTVESLEG 120 +L+K GLKEIEV FPSASQTDFDFVR+LI E RIPDDVTI VLT +RE+LIRRTVES+ G Sbjct: 61 MLVKIGLKEIEVAFPSASQTDFDFVRRLIEEKRIPDDVTIEVLTPAREDLIRRTVESVVG 120 Query: 121 AKKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESF 180 A++AI+HLYN+ APAFR+IVFNMSR+E+K +AV G +LV+ELTDA P TEW F+YSPE+F Sbjct: 121 ARRAIIHLYNATAPAFRRIVFNMSRDEVKALAVAGAKLVRELTDAHPETEWVFQYSPEAF 180 Query: 181 STTELDFSKDICDAVCETWGATPERKIILNLPSTVECATPNVYADQIEWMCRNLKDRAST 240 S TEL+FSK+ICDAV TW TPERK+I NLP TVE +TPNVYADQ+EWM RNL R S Sbjct: 181 SMTELEFSKEICDAVSATWQPTPERKMIFNLPGTVEASTPNVYADQVEWMHRNLARRDSI 240 Query: 241 IISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGL 300 +ISVHPHNDRG AVA+AELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLY+QG++PGL Sbjct: 241 LISVHPHNDRGCAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYSQGISPGL 300 Query: 301 DFSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPY 360 DFSDID VRQ VEECNQIPVHPRHPYVGDLV+TAFSGSHQDAIKKGFA+QQPDAIWEVPY Sbjct: 301 DFSDIDAVRQCVEECNQIPVHPRHPYVGDLVYTAFSGSHQDAIKKGFAQQQPDAIWEVPY 360 Query: 361 LPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGK 420 +P+DPADLGRSYDAVIRVNSQSGKGGM YLLEQEYGL LPRRLQIEFSRA+QR D TG Sbjct: 361 MPVDPADLGRSYDAVIRVNSQSGKGGMTYLLEQEYGLVLPRRLQIEFSRAVQRITDETGG 420 Query: 421 EIAASDIHAIFQREYLERVEPYVYRAHRMSEDTAKAESINIEVDIVRNGQPVTVRGSGNG 480 E+AA+DIH IF EYL V PY YR+H + E AE + I V++ ++G+PVT++G+GNG Sbjct: 421 EVAANDIHTIFNNEYLNAVTPYQYRSHHIDESQGNAE-VRIRVELEKDGKPVTLQGAGNG 479 Query: 481 PIDAFVHALGLDIKLMDFHEHAIGAGADAKAASYIELRLNEAPTGFGVGIDANIVTASFK 540 PIDAF++ALG+DI+LMDFHEHAIGAGA+A AASY+ELR+N T FGVGID NIVTAS + Sbjct: 480 PIDAFINALGMDIRLMDFHEHAIGAGANANAASYVELRVNNGSTRFGVGIDGNIVTASLR 539 Query: 541 AVLSAVNRQIAIS 553 AVLSAVNR IA++ Sbjct: 540 AVLSAVNRDIALA 552 >ref|YP_728252.1| 2-isopropylmalate synthase [Ralstonia eutropha H16] emb|CAJ94887.1| 2-Isopropylmalate synthase [Ralstonia eutropha H16] Length = 565 Score = 833 bits (2151), Expect = 0.0, Method: Composition-based stats. Identities = 402/567 (70%), Positives = 470/567 (82%), Gaps = 3/567 (0%) Query: 2 MLSNPAAKYRPFPAIDLPDRTWPSKVIDTPPIWMSTDLRDGNQALIEPMNAERKLRFFEL 61 MLSNPA KY P +D+PDRTWP++ I P WMSTDLRDGNQALIEPMN RKLRFFE Sbjct: 1 MLSNPATKYHPAATVDIPDRTWPARTITRAPRWMSTDLRDGNQALIEPMNPARKLRFFEQ 60 Query: 62 LLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPDDVTIIVLTQSREELIRRTVESLEGA 121 L+K GLKEIEV FP+ASQTDFDFVR LI E RIP+DV I+VLTQSRE+LIRRTVES+ GA Sbjct: 61 LVKIGLKEIEVAFPAASQTDFDFVRMLIEEQRIPEDVNIVVLTQSREDLIRRTVESVRGA 120 Query: 122 KKAIVHLYNSVAPAFRKIVFNMSREEIKNIAVTGTRLVKELTDARPGTEWRFEYSPESFS 181 +A VHLYN +APA+R+IVFN SREEIK +AV+GTRL++ LTDA PGT W +EYSPE+FS Sbjct: 121 ARATVHLYNPIAPAWRRIVFNASREEIKEVAVSGTRLIRSLTDAMPGTAWAYEYSPETFS 180 Query: 182 TTELDFSKDICDAVCETWGATPERKIILNLPSTVECATPNVYADQIEWMCRNLKDRASTI 241 ELDFS ++ DAV W A P+R +ILNLP+TVEC+TPNV+ADQ+EWM R L R Sbjct: 181 LAELDFSLEVSDAVSAAWQAGPDRPMILNLPTTVECSTPNVFADQVEWMHRRLARREHIA 240 Query: 242 ISVHPHNDRGTAVASAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVNPGLD 301 +SVHPHNDRGTAVA+AELA+MAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGV PGLD Sbjct: 241 LSVHPHNDRGTAVAAAELALMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVAPGLD 300 Query: 302 FSDIDVVRQVVEECNQIPVHPRHPYVGDLVFTAFSGSHQDAIKKGFAKQQPDAIWEVPYL 361 FSDID VRQ VE CNQ+PVHPRHPYVGDLVFTAFSGSHQDAI+KGFA+QQPD IWEVPYL Sbjct: 301 FSDIDAVRQCVEHCNQLPVHPRHPYVGDLVFTAFSGSHQDAIRKGFAQQQPDGIWEVPYL 360 Query: 362 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQEYGLALPRRLQIEFSRAIQREADATGKE 421 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQ +G+ +PRRLQIEFSRA+Q D TG E Sbjct: 361 PIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQVHGIYMPRRLQIEFSRAVQAMTDDTGLE 420 Query: 422 IAASDIHAIFQREYLERVEPYVYRAHRMSEDTAKAESINIEVDIVRNGQPVTVRGSGNGP 481 +A D+H +FQREYL + P Y +H++ D+ A IEV +VR+G+P +VRG+GNGP Sbjct: 421 ASADDLHGLFQREYLAQQTPLRYVSHQLVSDSGGA--TEIEVQMVRDGKPCSVRGTGNGP 478 Query: 482 IDAFVHALGLDIKLMDFHEHAIGAGADAKAASYIELRLNEAPTGFGVGIDANIVTASFKA 541 IDAFV+AL L +++MD+HEHA+ AGADA+AA Y+E+R+ ++PTGFG GIDAN+VTAS +A Sbjct: 479 IDAFVNALDLPVRVMDYHEHAMSAGADARAACYVEVRVGDSPTGFGAGIDANLVTASLRA 538 Query: 542 VLSAVNRQIAISESANQA-GSAQAKAA 567 V+S VNR + A QA SA+A A Sbjct: 539 VISGVNRHLQAGFGAAQAPQSAEAADA 565