BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4233.p (518 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAQ17383.1| typeIsecretion system protein [Ralstonia solanac... 281 3e-73 ref|YP_002220723.1| type I secretion membrane fusion protein, Hl... 202 8e-50 ref|NP_772932.1| hemolysin secretion protein D [Bradyrhizobium j... 201 4e-49 ref|NP_927980.1| hypothetical protein plu0635 [Photorhabdus lumi... 200 6e-49 ref|YP_236147.1| Type I secretion membrane fusion protein, HlyD ... 191 3e-46 >emb|CAQ17383.1| typeIsecretion system protein [Ralstonia solanacearum MolK2] Length = 488 Score = 281 bits (718), Expect = 3e-73, Method: Composition-based stats. Identities = 175/446 (39%), Positives = 261/446 (58%), Gaps = 19/446 (4%) Query: 85 PVPG-RPVLWGLLCLLVVLSIWAYLARIDVAASAAGKLVPEGKTQLLHATEMSLVRAIHA 143 P P R V+W ++ L+V+ +W+ + +I V A+A GK +P+G+ + + E S ++AIH Sbjct: 50 PSPAHRKVMWSIVGLVVISIVWSCIGQIAVVATAPGKFIPDGRIKQIQPLEASTIKAIHV 109 Query: 144 REGQRVKAGDLIVELDDTINRAEYKAVTQKLIQLRLERERLQAELDEREPVFDVAD---- 199 +EGQ V+AGDL++ELD I+ A +A + K RLE+ RL AEL P + AD Sbjct: 110 QEGQHVRAGDLLLELDPAISGAALQASSDKYAYNRLEQARLAAELTHAAPDYRNADPAWV 169 Query: 200 ALPESI-AAQRALFTAQRHAHQSRVAEAERNRQHRLMQQAAATALIAKLQANLQSARERV 258 AL ES AA+ A +TA+R +S+V E + H L AAA A + K + N + ER Sbjct: 170 ALQESTRAAREAAYTAKREQARSQVEE----KTHSL---AAAQATLTKYRENTAISEERE 222 Query: 259 ERVRPYAGE-SIAHFEYLRLQDNANSLAGELASQRASAQAARQEHLAAEQRLQFLEAEHR 317 RP +I+ +YL+L+ S +LA+Q + R A++RL +E +H+ Sbjct: 223 SSARPLVESGAISRVDYLQLKQELTSNRNDLAAQSKTVDEQRAALQEAQKRLAEVEHDHQ 282 Query: 318 SSLLAQTQEKAALMVAAQSDQARIEQLLRQKRLRSPVDGQVQNLSVATLGGAVTPGQVVA 377 + + Q +K A + D + QL K L++PVDG VQ ++V T+GG V P Q + Sbjct: 283 AEVYGQLSDKVANEPGLRGDMEKSRQLHDLKWLKAPVDGWVQKINVTTVGGVVNPAQTLM 342 Query: 378 SIVPEGMPLLIEAALSNEDAGFVRVGQRVDIKVDAFPYQQYGTLPGVVTWISPDAEPRGA 437 +IVP+GMPL++EA LSN+D G+V+VGQ V++KVD FP+Q+YGTL G + W+SPDAE + + Sbjct: 343 TIVPDGMPLVVEATLSNDDIGYVKVGQPVELKVDTFPFQKYGTLKGTLVWVSPDAEEKSS 402 Query: 438 GGNDAAAVATGMEPSSP-----PARGGLMYRMHIKPESTSLMRNGARHLVGIGMSVQADI 492 A G +P + G +Y++ I+ E + + +G + GM+VQADI Sbjct: 403 ASISDENGAGGSQPEKAIQNVKSVKDGYVYKVRIRAERSGFVVDGKPAALQAGMTVQADI 462 Query: 493 ITDRRRVIEFLLAPIIKNLDEGLKAR 518 TDRRRVIEF L+PIIK L E R Sbjct: 463 ATDRRRVIEFFLSPIIKYLSESTTVR 488 >ref|YP_002220723.1| type I secretion membrane fusion protein, HlyD family [Acidithiobacillus ferrooxidans ATCC 53993] ref|YP_002427069.1| type I secretion membrane fusion protein, HlyD family [Acidithiobacillus ferrooxidans ATCC 23270] gb|ACH84516.1| type I secretion membrane fusion protein, HlyD family [Acidithiobacillus ferrooxidans ATCC 53993] gb|ACK79070.1| type I secretion membrane fusion protein, HlyD family [Acidithiobacillus ferrooxidans ATCC 23270] Length = 465 Score = 202 bits (515), Expect = 8e-50, Method: Composition-based stats. Identities = 148/441 (33%), Positives = 222/441 (50%), Gaps = 20/441 (4%) Query: 80 PGPWEPVPGRPVLWGLLCLLVVLSIWAYLARIDVAASAAGKLVPEGKTQLLHATEMSLVR 139 P PW+ +LW LL LL V +W+YL +I++ A+A G+ +P+G+ +++ + V Sbjct: 43 PAPWQ----HWILWTLLALLFVALLWSYLGKINIVATATGQFIPDGRVKVVQPLSTATVE 98 Query: 140 AIHAREGQRVKAGDLIVELDDTINRAEYKAVTQKLIQLRLERERLQAELDEREPVFDVAD 199 I+ +G+ V G L+V L+ I +A +A Q+L L++ R+ A+L R + + Sbjct: 99 RIYVHDGETVHKGQLLVSLNPAITQANLQATQQELAFRNLQQARIHAQLSGRSAIAPASG 158 Query: 200 ALPESIAAQRALFTAQRHAHQSRVAEAERNRQHRLMQQAAATALIAKLQANLQSARERVE 259 PE I ++AL AQ +QSR+ A+ +A AL LQ +V Sbjct: 159 TNPEYIRMEKALQAAQEADYQSRLQAAQSRADQNATALNSARALAQSLQNQEALLHPQVV 218 Query: 260 RVRPYAGE-SIAHFEYLRLQDNANSLAGELASQRASAQAARQEHLAAEQRLQFLEAEHRS 318 A +I EY + L +A + Q + Q ++ ++A + + Sbjct: 219 ADASLAQSGAIPRMEYENARQKELGLQSRIAQNQGDIARYLQRQVEYRQDIEGIKANYHA 278 Query: 319 SLLAQTQEKAALMVAAQSDQARIEQLLRQKRLRSPVDGQVQNLSVATLGGAVTPGQVVAS 378 +LL E A +++ Q+ Q L LRSPVDG VQ+L+V +G VT Q V S Sbjct: 279 TLLHSLGENAQSLISLQAQQTHASHDLSLHELRSPVDGIVQDLAVRNVGEVVTAAQPVVS 338 Query: 379 IVPEGMPLLIEAALSNEDAGFVRVGQRVDIKVDAFPYQQYGTLPGVVTWISPDA-EPRGA 437 IVP G PL++EA L + FV VGQ +KV A+P++QYG L GVV ISPDA P G+ Sbjct: 339 IVPSGTPLVVEAYLPDAHMAFVHVGQTARVKVSAYPFEQYGALQGVVQKISPDAVSPSGS 398 Query: 438 GGNDAAAVATGMEPSSPPARGGLMYRMHIKPESTSLMRNGARHLVGIGMSVQADIITDRR 497 G TG GL YR+ I LM +G + GM+V A+I T RR Sbjct: 399 G-----KALTGE---------GLSYRVRITVPHPYLMVHGVSVKMRPGMTVSANINTGRR 444 Query: 498 RVIEFLLAPIIKNLDEGLKAR 518 R+I+F L P+ ++ DEGL R Sbjct: 445 RIIQFFLGPLFRDWDEGLSVR 465 >ref|NP_772932.1| hemolysin secretion protein D [Bradyrhizobium japonicum USDA 110] dbj|BAC51557.1| hlyD [Bradyrhizobium japonicum USDA 110] Length = 513 Score = 201 bits (510), Expect = 4e-49, Method: Composition-based stats. Identities = 154/470 (32%), Positives = 241/470 (51%), Gaps = 15/470 (3%) Query: 56 RHRLRRRAQSVGGSGNTREAWKNLPGPWEPVPGRPVLWGLLCLLVVLSIWAYLARIDVAA 115 R +RRR + E ++ P P GR + ++ + V +WA L +D+ A Sbjct: 52 RAEVRRREHEIAFLPAALEITESPPSPI----GRAIGGSIIAVFCVALLWASLGSVDIVA 107 Query: 116 SAAGKLVPEGKTQLLHATEMSLVRAIHAREGQRVKAGDLIVELDDTINRAEYKAVTQKLI 175 +A GK+VP G+T+L+ E +VRAIH R+GQ VKAGD+++ELD T+ A+ + L+ Sbjct: 108 TATGKIVPGGRTKLIQPFETGVVRAIHVRDGQSVKAGDVLIELDPTMTEADQERQRSDLV 167 Query: 176 QLRLERERLQAELDEREPVFDVADALPESIAA---QRALFTAQRHAHQSRVAEAERNRQH 232 L+ RL+A L +P+ S+A R +QR +++AE ER + Sbjct: 168 AAELDVARLRAALTS-DPLAAFRSPQGASVAEVEMHRQFLISQRAEQSAKLAEIERQQGQ 226 Query: 233 RLMQQAAATALIAKLQANLQSARERVERVRPYAGESIA-HFEYLRLQDNANSLAGELASQ 291 + ++A +A +AKLQA + +ERV+ + +++A YL L +L Q Sbjct: 227 KEAERATTSASVAKLQATIPVLQERVDIRKGLVDKALASKVIYLSEYQELVGLQQDLVLQ 286 Query: 292 RASAQAARQEHLAAEQRLQFLEAEHRSSL---LAQTQEKAALMVAAQSDQARIEQLLRQK 348 ++ A ++ + AE+R + LA+ ++KAA +A + + E+ + + Sbjct: 287 QSRLGEADAAIALLKETKERTAAEYRRATYDALAKAEQKAA---SAAQEVVKAERRTKLQ 343 Query: 349 RLRSPVDGQVQNLSVATLGGAVTPGQVVASIVPEGMPLLIEAALSNEDAGFVRVGQRVDI 408 L +PVDG VQ L+V T+GG VTP Q +A +VP L IEA LSN D GFV GQ+ +I Sbjct: 344 HLTAPVDGVVQQLAVHTVGGVVTPAQALAIVVPSESQLEIEAMLSNRDIGFVHPGQKAEI 403 Query: 409 KVDAFPYQQYGTLPGVVTWISPDAEPRGAGGNDAAAVATGMEPSSPPARGGLMYRMHIKP 468 KVD F + +YG L G V +S DA R G + + SS P L Y I Sbjct: 404 KVDTFNFTRYGLLNGDVLSVSTDAITRDRQGGPNDRASGTAQGSSEPKGQELEYAARISL 463 Query: 469 ESTSLMRNGARHLVGIGMSVQADIITDRRRVIEFLLAPIIKNLDEGLKAR 518 + T + +G GM+V +I T RR+I +LL+P+ + E L+ R Sbjct: 464 DRTHMQVEDKLVKLGPGMAVTVEIKTGARRIISYLLSPLARYKQESLRER 513 >ref|NP_927980.1| hypothetical protein plu0635 [Photorhabdus luminescens subsp. laumondii TTO1] emb|CAE12930.1| unnamed protein product [Photorhabdus luminescens subsp. laumondii TTO1] Length = 471 Score = 200 bits (508), Expect = 6e-49, Method: Composition-based stats. Identities = 147/447 (32%), Positives = 235/447 (52%), Gaps = 33/447 (7%) Query: 82 PWEPVPGRPVLWGLLCLLVVLSIWAYLARIDVAASAAGKLVPEGKTQLLHATEMSLVRAI 141 P P P R W +L V+ +WAY+ +I+V A A GK+VP GKT+L+ ++E ++VRAI Sbjct: 48 PVHPAP-RIFTWTILGFAVLALLWAYIGKIEVVAIAPGKVVPNGKTKLIQSSETAVVRAI 106 Query: 142 HAREGQRVKAGDLIVELDDTINRAEYKAVTQKLIQLRLERERLQAELD----EREP---V 194 H +GQ VK G+L+VELD T A+ + +L+ R++ R A LD ++ P V Sbjct: 107 HVNDGQSVKVGELLVELDPTAASADVSRIQSELLAARIDSARSAAMLDAINQQKSPASLV 166 Query: 195 FDVADALPESIAAQRALFTAQRHAHQSRVAEAERNRQHRLMQQAAATALIAKLQANLQSA 254 + +A PE + + + Q ++S + + R + +A A +A L+ L A Sbjct: 167 GTIDNANPEQVLSAQRWLQGQYQEYRSNLELVDAEILQRSAEIQSAQAQVASLRKTLPIA 226 Query: 255 RERVERV-RPYAGESIAHFEYLRLQDNANSLAGELASQRASAQAARQEHLAAEQRLQFLE 313 E R + ++ EYL + L +L+ Q+AS A +R + + Sbjct: 227 TRLAEDYQRLLKKQYVSRHEYLEKEQTRLDLQRQLSVQQASVLQHAAAQDEARRRREAVV 286 Query: 314 AEHRSSLLAQTQE--KAALMVAAQSDQARIEQLLRQKRLRSPVDGQVQNLSVATLGGAVT 371 A++R ++L QE K + + +AR ++ L L++PV G VQ L++ T+GG VT Sbjct: 287 AQNRRAMLDLQQEANKKVATLVQELAKARYQETLTS--LKAPVAGTVQQLAIHTVGGVVT 344 Query: 372 PGQVVASIVPEGMPLLIEAALSNEDAGFVRVGQRVDIKVDAFPYQQYGTLPGVVTWISPD 431 P Q + IVP + +EA L N+D GFV VGQ V +KV+ F + +YGT+ G V +S D Sbjct: 345 PAQPLMVIVPADQSVEVEARLENKDVGFVHVGQPVTVKVETFTFTKYGTVDGEVLNVSND 404 Query: 432 AEPRGAGGNDAAAVATGMEPSSPPARGGLMYRMHIKPESTSLMRNGARHLVGIGMSVQAD 491 A + GL+Y I+ +S +M NG R + GMS+ A+ Sbjct: 405 A--------------------IEDEKHGLIYSSRIRLKSDHVMVNGQRVPLSPGMSITAE 444 Query: 492 IITDRRRVIEFLLAPIIKNLDEGLKAR 518 I TD+RRVI++ L+P+ +++DE L+ R Sbjct: 445 IKTDQRRVIDYFLSPLQQHIDESLRER 471 >ref|YP_236147.1| Type I secretion membrane fusion protein, HlyD [Pseudomonas syringae pv. syringae B728a] gb|AAY38109.1| Type I secretion membrane fusion protein, HlyD [Pseudomonas syringae pv. syringae B728a] Length = 473 Score = 191 bits (485), Expect = 3e-46, Method: Composition-based stats. Identities = 148/448 (33%), Positives = 229/448 (51%), Gaps = 35/448 (7%) Query: 82 PWEPVPGRPVLWGLLCLLVVLSIWAYLARIDVAASAAGKLVPEGKTQLLHATEMSLVRAI 141 P P P R +W ++ + +WA L +IDV A+A+GK++P GKT+ + ++E ++V+AI Sbjct: 50 PSHPAP-RVFMWAIMFFAALALLWACLGKIDVVATASGKIIPSGKTKTIQSSETAVVKAI 108 Query: 142 HAREGQRVKAGDLIVELDDTINRAEYKAVTQKLIQLRLERERLQAELD---EREPVFD-- 196 H R+GQ VKAG L++ELD T A+ V L+ R++ R A LD +R+P D Sbjct: 109 HVRDGQSVKAGQLLLELDSTSADADVGRVRSDLLAARIDSARAAAMLDAINQRKPPRDLT 168 Query: 197 --VADALPESIAAQRALFTAQRHAHQSRVAEAERNRQHRLMQQAAATALIAKLQANLQSA 254 + DA P + A Q ++S + + Q R AA + LQ L A Sbjct: 169 GTILDADPMHVLAAERWLQGQYQEYRSSLDLVDAEIQQRQADIQAARIQVMSLQKTLPIA 228 Query: 255 RERVERVRPY-AGESIAHFEYLRLQDNANSLAGELASQRAS---AQAARQEHLAAEQRLQ 310 + + IA YL + L +L+ Q+AS + AARQE AE+R + Sbjct: 229 TKLASDYENLLKKQYIARHAYLEKEQARLDLERQLSVQQASVLQSTAARQE---AERRRE 285 Query: 311 FLEAEHRSSLLAQTQEKAALMVAAQSDQARIEQLLRQKRLRSPVDGQVQNLSVATLGGAV 370 + A+ R ++L Q+ + + D + L +PVDG VQ L+V T+GG V Sbjct: 286 GVVAQTRRAMLDLLQQADQKIASFNQDLTKARYQEDLTTLEAPVDGTVQQLAVHTVGGVV 345 Query: 371 TPGQVVASIVPEGMPLLIEAALSNEDAGFVRVGQRVDIKVDAFPYQQYGTLPGVVTWISP 430 TP Q + +VP+G P+ +EA L N+D GFVR GQ V +KV+ F + +YGT+ G V +S Sbjct: 346 TPAQPLMVLVPDGQPVEVEAMLENKDVGFVRAGQAVTVKVETFTFTKYGTIEGEVISVSN 405 Query: 431 DAEPRGAGGNDAAAVATGMEPSSPPARGGLMYRMHIKPESTSLMRNGARHLVGIGMSVQA 490 DA + GL+Y I+ S +L NG + GM+V A Sbjct: 406 DA--------------------IEDEKRGLIYSSKIRLNSDTLNVNGVDIKLSPGMAVTA 445 Query: 491 DIITDRRRVIEFLLAPIIKNLDEGLKAR 518 ++ T++RRVIE+ L+P+ ++ E + R Sbjct: 446 EVTTNKRRVIEYFLSPLQQHASESFRER 473