BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4208.p (312 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776739.1| 2-keto-4-pentenoate hydratase [Herbaspirillu... 400 e-109 ref|YP_004618283.1| 2-keto-4-pentenoate hydratase [Ramlibacter t... 184 2e-44 ref|YP_001770730.1| hydratase/decarboxylase [Methylobacterium sp... 179 5e-43 ref|YP_002942039.1| 2-oxopent-4-enoate hydratase [Variovorax par... 168 9e-40 ref|YP_729041.1| 2-oxopent-4-enoate hydratase [Ralstonia eutroph... 158 8e-37 >ref|YP_003776739.1| 2-keto-4-pentenoate hydratase [Herbaspirillum seropedicae SmR1] gb|ADJ64831.1| 2-keto-4-pentenoate hydratase protein [Herbaspirillum seropedicae SmR1] Length = 247 Score = 400 bits (1028), Expect = e-109, Method: Composition-based stats. Identities = 211/246 (85%), Positives = 222/246 (90%) Query: 66 MHIDAATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLRRLGPIGGWKVGSKGDGSL 125 MHIDAATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQL RLGP+GGWKVGSKGDG Sbjct: 1 MHIDAATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLLRLGPVGGWKVGSKGDGGT 60 Query: 126 PACAPLPASGVLSSGTQLSGPQWRMRGLEVELALRVGRDYAPGEQVPDEAELRTVFDAIL 185 P CAPLPASGVL SGT L+GPQWRMRGLEVELALRVGRDY PGEQ+PDEAELRTVFDAIL Sbjct: 61 PGCAPLPASGVLPSGTTLAGPQWRMRGLEVELALRVGRDYDPGEQLPDEAELRTVFDAIL 120 Query: 186 PAIEVVETRLGHPPCTNPWAGMADLQSHGALVLGQAQPLPATLSLRQIAASLHIDGQEIT 245 PAIEVVETRLGHPPCTNPWAGMADLQSHGALV G Q LP LSLRQI ASL IDGQ+++ Sbjct: 121 PAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPDLLSLRQIEASLSIDGQQVS 180 Query: 246 RTTGGNPADLWPMLGWLALHCARRGQPWCQGQVITTGSCTGLQLARAGRQIEGALAGIGA 305 TTGGNPADLWP+LGWLALHCARRGQPW GQ+ITTGSCTGLQ+AR GR++E AL GIGA Sbjct: 181 HTTGGNPADLWPLLGWLALHCARRGQPWRAGQIITTGSCTGLQMARGGRRVEAALHGIGA 240 Query: 306 VSFSFA 311 V FA Sbjct: 241 VWLDFA 246 >ref|YP_004618283.1| 2-keto-4-pentenoate hydratase [Ramlibacter tataouinensis TTB310] gb|AEG92264.1| 2-keto-4-pentenoate hydratase-like protein [Ramlibacter tataouinensis TTB310] Length = 250 Score = 184 bits (466), Expect = 2e-44, Method: Composition-based stats. Identities = 114/248 (45%), Positives = 149/248 (60%), Gaps = 2/248 (0%) Query: 65 RMHIDAATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLRRLGPIGGWKVGSKGDGS 124 R + A + L +AR G + + A AYA+QDA LR LGP GGWKVG+ + Sbjct: 3 RNDFETAGRALAEARRGGGKVPHASIPAAQATDAYAIQDATLRVLGPHGGWKVGAASAQA 62 Query: 125 LPACAPLPASGVLSSGTQLSGPQWRMRGLEVELALRVGRDYAPGEQVPDEAELRTVFDAI 184 +P CAPLP+SG+L G L+ P +RG+E+E+A+R+GRD P +V AE+ DA+ Sbjct: 63 MPNCAPLPSSGLLPGGATLARPGAALRGIELEVAVRLGRDLLPDGRVLPAAEIVAAVDAL 122 Query: 185 LPAIEVVETRLGHPPCTNPWAGMADLQSHGALVLGQAQPL-PATLSLRQIAASLHIDGQE 243 LPAIEVV+TRL + P +ADLQSH LV G A L PA L L + L DG Sbjct: 123 LPAIEVVDTRLAEGRQSPPPVQLADLQSHDVLVTGAASALKPARLDLASLRCRLWFDGSP 182 Query: 244 ITRTTGGNPA-DLWPMLGWLALHCARRGQPWCQGQVITTGSCTGLQLARAGRQIEGALAG 302 T GG+P D+W +L WLA+HC RG P GQV+TTGSCTGL A A ++ G + G Sbjct: 183 AADTVGGHPTRDIWSLLAWLAVHCEARGLPLRAGQVVTTGSCTGLLFAPASARVRGEIDG 242 Query: 303 IGAVSFSF 310 +G V+ SF Sbjct: 243 LGEVALSF 250 >ref|YP_001770730.1| hydratase/decarboxylase [Methylobacterium sp. 4-46] gb|ACA18296.1| Hydratase/decarboxylase [Methylobacterium sp. 4-46] Length = 238 Score = 179 bits (454), Expect = 5e-43, Method: Composition-based stats. Identities = 120/238 (50%), Positives = 151/238 (63%), Gaps = 5/238 (2%) Query: 75 LLDARHSGLALDWRRLAVGDAETAYAVQDAQLRRLGPIGGWKVGSKGDGSLPACAPLPAS 134 LL A G + WR +A D AYAVQDA RLGP+GGWKVG+ ++P AP PA+ Sbjct: 4 LLLAARGGARVPWRAVAPADPAGAYAVQDAVAARLGPVGGWKVGAANPEAVPNAAPCPAA 63 Query: 135 GVLSSGTQLSGPQWRMRGLEVELALRVGRDYAPGEQVPDEAELRTVFDAILPAIEVVETR 194 + SG L GP+WR+RG+E E+ LR+GRD + AEL +A+LP IEVVETR Sbjct: 64 CLRGSGAVLDGPEWRLRGIEAEVGLRLGRDL---DGAAPRAELAAAVEAVLPVIEVVETR 120 Query: 195 LGHPPCTNPWAGMADLQSHGALVLGQAQPL-PATLSLRQIAASLHIDGQEITRTTGGNPA 253 L +P A +ADLQSHGAL+LG L PA L LR +AA L +DG+ + T GGNPA Sbjct: 121 LDGWGEADPLARLADLQSHGALILGPPSRLDPAALDLRTVAADLSVDGRAVAATRGGNPA 180 Query: 254 -DLWPMLGWLALHCARRGQPWCQGQVITTGSCTGLQLARAGRQIEGALAGIGAVSFSF 310 D+W +L WLA H A RG+P GQV+TTGSCTG+ G ++ LAGIGAV+ F Sbjct: 181 EDVWRLLAWLADHAAARGRPLRAGQVVTTGSCTGMLFCAQGTRVAAELAGIGAVAMRF 238 >ref|YP_002942039.1| 2-oxopent-4-enoate hydratase [Variovorax paradoxus S110] gb|ACS16773.1| 2-oxopent-4-enoate hydratase [Variovorax paradoxus S110] Length = 254 Score = 168 bits (426), Expect = 9e-40, Method: Composition-based stats. Identities = 100/215 (46%), Positives = 134/215 (62%), Gaps = 2/215 (0%) Query: 98 AYAVQDAQLRRLGPIGGWKVGSKGDGSLPACAPLPASGVLSSGTQLSGPQWRMRGLEVEL 157 AYA+QDA L +GP+GGWKVG++ G P CAPLPA G+L G +L GP WR+RG+E E+ Sbjct: 40 AYAIQDATLAAIGPVGGWKVGARAPGQEPTCAPLPAEGLLPGGVRLQGPAWRLRGIEAEV 99 Query: 158 ALRVGRDYAPGEQVPDEAELRTVFDAILPAIEVVETRLGHPPCTNPWAGMADLQSHGALV 217 ++G D P A++ +++LP IEVVETRL + A +ADL SHGALV Sbjct: 100 GFQLGADLPPRAAPYTLADVAGAVESVLPVIEVVETRLADWFGAHANALLADLLSHGALV 159 Query: 218 LGQAQPL-PATLSLRQIAASLHIDGQEITRTTGGNPA-DLWPMLGWLALHCARRGQPWCQ 275 LGQ QP PA L++ A+LH DGQ + T G +P+ D +L WLA HCA RG Sbjct: 160 LGQRQPFAPAWFDLQRAEAALHFDGQTVAHTVGEHPSPDAGALLVWLANHCAARGAGLKA 219 Query: 276 GQVITTGSCTGLQLARAGRQIEGALAGIGAVSFSF 310 GQ++T+GSCTG+ A AG + + + V+ SF Sbjct: 220 GQIVTSGSCTGMLFASAGTAVRVEIGELAPVALSF 254 >ref|YP_729041.1| 2-oxopent-4-enoate hydratase [Ralstonia eutropha H16] emb|CAJ95676.1| 2-Oxopent-4-enoate hydratase [Ralstonia eutropha H16] Length = 254 Score = 158 bits (400), Expect = 8e-37, Method: Composition-based stats. Identities = 104/243 (42%), Positives = 141/243 (58%), Gaps = 2/243 (0%) Query: 70 AATQLLLDARHSGLALDWRRLAVGDAETAYAVQDAQLRRLGPIGGWKVGSKGDGSLPACA 129 A Q+L AR G +D L +YAVQDA L +GPIGGWKVG++G G PACA Sbjct: 12 ALAQMLCVARTRGERVDPALLHPLAHGGSYAVQDATLAMIGPIGGWKVGARGPGQEPACA 71 Query: 130 PLPASGVLSSGTQLSGPQWRMRGLEVELALRVGRDYAPGEQVPDEAELRTVFDAILPAIE 189 PLPA+G+L G L GP WR+RG+EVE+A +G P + + ++ ++PAIE Sbjct: 72 PLPAAGLLPDGVGLRGPAWRLRGIEVEVAFVLGASLPPRGRPYERRDIEAALALVVPAIE 131 Query: 190 VVETRLGHPPCTNPWAGMADLQSHGALVLGQAQPLPAT-LSLRQIAASLHIDGQEITRTT 248 VVE+RL A +ADL SHGALV G+ P L L + A+L + + T Sbjct: 132 VVESRLEDWLGAGSDAQLADLLSHGALVTGRPTPFAREWLHLGALEATLRFGRRTVAHTV 191 Query: 249 GGNPA-DLWPMLGWLALHCARRGQPWCQGQVITTGSCTGLQLARAGRQIEGALAGIGAVS 307 GG+ + D+ +L WLA HCA RG GQV+TTG+CTGL A AG ++ + G+G ++ Sbjct: 192 GGHQSPDVGALLVWLANHCAARGAGLKAGQVVTTGTCTGLLFASAGTRVSAGIEGLGTLA 251 Query: 308 FSF 310 F Sbjct: 252 AWF 254