BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4195.p (319 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776725.1| D-3-phosphoglycerate dehydrogenase [Herbaspi... 586 e-165 ref|YP_003409482.1| NAD-binding D-isomer specific 2-hydroxyacid ... 294 1e-77 ref|YP_003322014.1| D-isomer specific 2-hydroxyacid dehydrogenas... 292 4e-77 ref|YP_003991614.1| d-isomer specific 2-hydroxyacid dehydrogenas... 232 6e-59 ref|ZP_07930558.1| D-isomer specific 2-hydroxyacid dehydrogenase... 226 3e-57 >ref|YP_003776725.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] gb|ADJ64817.1| D-3-phosphoglycerate dehydrogenase protein [Herbaspirillum seropedicae SmR1] Length = 319 Score = 586 bits (1510), Expect = e-165, Method: Composition-based stats. Identities = 310/316 (98%), Positives = 313/316 (99%) Query: 1 MKVLITDYDFPDVDLELALYKEAGVEVVTAQCRTEEEVIAASAGCQGLLVQYAPVNAKVF 60 MKVLITDYDFPDVDLELALYKEAGVEVVTAQCRTEEEVIAASAGCQGLLVQYAPVNAKVF Sbjct: 1 MKVLITDYDFPDVDLELALYKEAGVEVVTAQCRTEEEVIAASAGCQGLLVQYAPVNAKVF 60 Query: 61 AARPEIRIASRYGAGFDTINTADAAAHGVWVANSPDYGVGEVATHALAMALDLIRNITVY 120 AARPEIRIASRYGAGFDTINTADAAAHGVWVANSPDYGVGEVATHALAMALDLIRNITVY Sbjct: 61 AARPEIRIASRYGAGFDTINTADAAAHGVWVANSPDYGVGEVATHALAMALDLIRNITVY 120 Query: 121 DRAVKAGEWHYTTAGIIPRASDLTIGIIGLGRIGKRMAHISRNLFKRVIAYDPYIIDGDF 180 DRAVKAGEWHYTTAGIIPRASDLTIGIIGLGRIGKRMAHISRNLFKRVIAYDPYIIDGDF Sbjct: 121 DRAVKAGEWHYTTAGIIPRASDLTIGIIGLGRIGKRMAHISRNLFKRVIAYDPYIIDGDF 180 Query: 181 PAYVERVSREELFRQAHVVSLHTPLNDETRNMIDASLLNLLQPQSYLVNSARGGLIKIDD 240 PAYVERV REELFRQAHVVSLHTPLNDETRNMIDASLL+LLQ QSYLVNSARGGL+KIDD Sbjct: 181 PAYVERVGREELFRQAHVVSLHTPLNDETRNMIDASLLDLLQAQSYLVNSARGGLVKIDD 240 Query: 241 LLAALENGKLKGAALDVLPVEPPQTASAIVQHPRVLLSPHAAFFSDVAARELRRKAAQNL 300 LLAALENGKLKGAALDVLPVEPPQTASAIVQHPRVLLSPHAAFFSDVAARELRRKAA+NL Sbjct: 241 LLAALENGKLKGAALDVLPVEPPQTASAIVQHPRVLLSPHAAFFSDVAARELRRKAAKNL 300 Query: 301 IDWDRNGRPSYVVVQG 316 IDWDR GRPSYVVVQG Sbjct: 301 IDWDRTGRPSYVVVQG 316 >ref|YP_003409482.1| NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase protein [Geodermatophilus obscurus DSM 43160] gb|ADB75111.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Geodermatophilus obscurus DSM 43160] Length = 322 Score = 294 bits (753), Expect = 1e-77, Method: Composition-based stats. Identities = 151/316 (47%), Positives = 210/316 (66%) Query: 1 MKVLITDYDFPDVDLELALYKEAGVEVVTAQCRTEEEVIAASAGCQGLLVQYAPVNAKVF 60 ++VL+TD D+ +D+E + AG E+ TA+CRT EVI A+ +LVQYAP+ A+V Sbjct: 6 LRVLVTDADYGVLDIEEQVLAAAGHELRTARCRTPAEVIDAARDADAVLVQYAPITAEVL 65 Query: 61 AARPEIRIASRYGAGFDTINTADAAAHGVWVANSPDYGVGEVATHALAMALDLIRNITVY 120 A P +R+ SRYG G D ++T A A GVWV N PDYG EVA HA+A+ L L+RN+ + Sbjct: 66 DALPRLRLVSRYGVGVDVVDTDAARARGVWVCNVPDYGTTEVALHAVAVLLALLRNLPEH 125 Query: 121 DRAVKAGEWHYTTAGIIPRASDLTIGIIGLGRIGKRMAHISRNLFKRVIAYDPYIIDGDF 180 DR V AG W + G + R LT+G++GLGRIG+ + F V+ +DP++ D + Sbjct: 126 DRQVHAGRWDHRLGGRLRRPDGLTLGVVGLGRIGRTTMAGAAPWFGAVVGHDPHLPDDAW 185 Query: 181 PAYVERVSREELFRQAHVVSLHTPLNDETRNMIDASLLNLLQPQSYLVNSARGGLIKIDD 240 PA VER+ ELF ++ V+LH PL +TR ++ A LL + SYLVN+ARGGL+++D Sbjct: 186 PAGVERLGLAELFARSDAVTLHLPLTADTRGLVGADLLGRMPAGSYLVNTARGGLVQLDA 245 Query: 241 LLAALENGKLKGAALDVLPVEPPQTASAIVQHPRVLLSPHAAFFSDVAARELRRKAAQNL 300 +L ALE+G+L G LDV P EPP S ++ HPR LL+PH A++S+ A ELRRKAA N+ Sbjct: 246 VLQALEDGRLAGVGLDVFPTEPPPAGSPLLAHPRALLTPHVAWYSEEAEVELRRKAAGNV 305 Query: 301 IDWDRNGRPSYVVVQG 316 + W R GRP +VVV+G Sbjct: 306 VSWARTGRPDHVVVEG 321 >ref|YP_003322014.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Thermobaculum terrenum ATCC BAA-798] gb|ACZ41192.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Thermobaculum terrenum ATCC BAA-798] Length = 324 Score = 292 bits (748), Expect = 4e-77, Method: Composition-based stats. Identities = 152/313 (48%), Positives = 213/313 (68%) Query: 2 KVLITDYDFPDVDLELALYKEAGVEVVTAQCRTEEEVIAASAGCQGLLVQYAPVNAKVFA 61 KVLITD ++PD+ E L +EAG +V AQCR EEVI A+ L+VQYAP+ + V Sbjct: 5 KVLITDAEYPDLSDEQRLLEEAGCQVSIAQCRLPEEVILAARDAFALMVQYAPITSDVIH 64 Query: 62 ARPEIRIASRYGAGFDTINTADAAAHGVWVANSPDYGVGEVATHALAMALDLIRNITVYD 121 A P +++ SR G G D+++ A GVWVAN PDYGV EVA+HA AM L L+R++ D Sbjct: 65 ALPNLKLISRAGVGVDSVDLDAAKEKGVWVANVPDYGVHEVASHAAAMILSLLRHVCFLD 124 Query: 122 RAVKAGEWHYTTAGIIPRASDLTIGIIGLGRIGKRMAHISRNLFKRVIAYDPYIIDGDFP 181 R V+ G WHY + G I R S LT+G++GLGRIG+ +A + ++F RVI YDPY+ +P Sbjct: 125 RKVREGVWHYLSTGEIHRISSLTLGVVGLGRIGRTLASMMLHVFGRVIGYDPYLPANAWP 184 Query: 182 AYVERVSREELFRQAHVVSLHTPLNDETRNMIDASLLNLLQPQSYLVNSARGGLIKIDDL 241 V+RV +LF +++VVSLH PL ET NM++ L L+ SYLVN+ARGGL+ ++DL Sbjct: 185 EGVDRVDLRDLFMKSNVVSLHLPLTKETSNMVNREFLALMPVGSYLVNTARGGLVNMEDL 244 Query: 242 LAALENGKLKGAALDVLPVEPPQTASAIVQHPRVLLSPHAAFFSDVAARELRRKAAQNLI 301 + L++G L GAALDVLP EPP I+ HP+++++PH A++S+ + +ELR K A N++ Sbjct: 245 IYFLDSGHLAGAALDVLPKEPPVAGDPIIHHPKIIITPHVAWYSEESEKELRYKWAMNVV 304 Query: 302 DWDRNGRPSYVVV 314 + +NG P Y VV Sbjct: 305 AFLKNGSPLYPVV 317 >ref|YP_003991614.1| d-isomer specific 2-hydroxyacid dehydrogenase nad-binding protein [Caldicellulosiruptor hydrothermalis 108] gb|ADQ06245.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Caldicellulosiruptor hydrothermalis 108] Length = 332 Score = 232 bits (592), Expect = 6e-59, Method: Composition-based stats. Identities = 119/314 (37%), Positives = 190/314 (60%), Gaps = 1/314 (0%) Query: 1 MKVLITDYDFPDVDLELALYKEAGVEVVTAQCRTEEEVIAASAGCQGLLVQYAPVNAKVF 60 +KV++TD+++ D+ E ++ AG+ ++ AQCRTE+E+I GLL QYA + +V Sbjct: 4 LKVVVTDFEYSDLRYEEEVFNSAGITLIPAQCRTEDELITVCKEADGLLSQYAQITRRVI 63 Query: 61 AARPEIRIASRYGAGFDTINTADAAAHGVWVANSPDYGVGEVATHALAMALDLIRNITVY 120 + P+ ++ RYG G +TI+ A G+ V N PDY + EV+ HALA+ L L R I + Sbjct: 64 ESLPKCKVIGRYGVGVNTIDIEAATEKGICVVNVPDYCMDEVSDHALALILTLARKIVLL 123 Query: 121 DRAVKAGEWHYTTAGIIPRASDLTIGIIGLGRIGKRMAHISRNLFKRVIAYDPYIIDGDF 180 + +VK G W Y + I R +G++G GRI + +A ++++ ++ YDPYI D Sbjct: 124 NNSVKKGIWDYKISKPIYRLRGKVLGLVGFGRIPRTLAQKAKSIGFNILVYDPYISPNDA 183 Query: 181 PAY-VERVSREELFRQAHVVSLHTPLNDETRNMIDASLLNLLQPQSYLVNSARGGLIKID 239 AY + VS +EL Q+ +S+H PLN ET ++I L L++P ++L+N+ARG +I Sbjct: 184 EAYGAKLVSLDELMTQSDFISIHVPLNKETYHLIGPQELKLMKPTAFLINTARGPVIDEK 243 Query: 240 DLLAALENGKLKGAALDVLPVEPPQTASAIVQHPRVLLSPHAAFFSDVAARELRRKAAQN 299 L+ L ++ GAALDV VEP Q+ + +++ V+++PH A++S+ A ELR KAAQ Sbjct: 244 ALIEVLLEKRIAGAALDVTEVEPIQSDNPLLKMDNVIITPHVAWYSEEAEAELRTKAAQG 303 Query: 300 LIDWDRNGRPSYVV 313 + D P Y+V Sbjct: 304 IADVLLGYWPRYLV 317 >ref|ZP_07930558.1| D-isomer specific 2-hydroxyacid dehydrogenase [Anaerostipes sp. 3_2_56FAA] gb|EFV23279.1| D-isomer specific 2-hydroxyacid dehydrogenase [Anaerostipes sp. 3_2_56FAA] Length = 322 Score = 226 bits (577), Expect = 3e-57, Method: Composition-based stats. Identities = 128/313 (40%), Positives = 189/313 (60%), Gaps = 1/313 (0%) Query: 1 MKVLITDYDFPDVDLELALYKEAGVEVVTAQCRTEEEVIAASAGCQGLLVQYAPVNAKVF 60 MKV+ITD D ++D+E + +AG+E RTE+EVI AG +VQYA ++ KV Sbjct: 1 MKVIITDCDHENIDIETKVLSDAGIEFKLTCARTEDEVIEQCAGADIFIVQYANISEKVM 60 Query: 61 AARPEIRIASRYGAGFDTINTADAAAHGVWVANSPDYGVGEVATHALAMALDLIRNITVY 120 A PE++ RYG G DT++ AA +G+ V N PDYG+ EVA HALA+ L ++R Sbjct: 61 KALPELKYVVRYGVGVDTVDLKAAAEYGIQVGNVPDYGMNEVADHALALLLMMLRKTDQM 120 Query: 121 DRAVKAGEWHYTTAGIIPRASDLTIGIIGLGRIGKRMAHISRNLFKRVIAYDPYIIDGDF 180 +R K W YT + I R S+ T+G++GLGRIG+ A + L RVI YDP + G+ Sbjct: 121 NRFTKTTGWDYTQSIPIRRFSEQTVGVVGLGRIGRNFAEKAAALGFRVIGYDPMVNQGER 180 Query: 181 PAYVERVSREELFRQAHVVSLHTPLNDETRNMIDASLLNLLQPQSYLVNSARGGLIKIDD 240 E VS EEL +Q+ VSLH P D +N+ D +++ + +VN+ARGG+I +D Sbjct: 181 IGNAEAVSFEELLKQSDAVSLHCPA-DGNQNLFDMEAFRVMKDSAVIVNAARGGIINEED 239 Query: 241 LLAALENGKLKGAALDVLPVEPPQTASAIVQHPRVLLSPHAAFFSDVAARELRRKAAQNL 300 L AL+ + GAALD + EP T S+I ++ ++++PH A++S+ AA EL+RKAA+ Sbjct: 240 LDRALKEKVIAGAALDCMKSEPMMTDSSIFENENLIVTPHMAWYSEEAAMELKRKAAEEA 299 Query: 301 IDWDRNGRPSYVV 313 + + + Y V Sbjct: 300 VRFAKGEAIHYPV 312