BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4161.p (335 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_001901123.1| dienelactone hydrolase [Ralstonia pickettii ... 211 1e-52 ref|YP_002983187.1| dienelactone hydrolase [Ralstonia pickettii ... 206 5e-51 ref|ZP_07675117.1| carboxymethylenebutenolidase [Ralstonia sp. 5... 205 1e-50 ref|YP_001206576.1| putative alpha/beta-hydrolases superfamily p... 163 4e-38 ref|YP_587410.1| alpha/beta-hydrolase [Cupriavidus metallidurans... 160 3e-37 >ref|YP_001901123.1| dienelactone hydrolase [Ralstonia pickettii 12J] gb|ACD28691.1| dienelactone hydrolase [Ralstonia pickettii 12J] Length = 349 Score = 211 bits (537), Expect = 1e-52, Method: Composition-based stats. Identities = 129/298 (43%), Positives = 175/298 (58%), Gaps = 14/298 (4%) Query: 41 FDSVTPKRIMDFVERA-ANEKVSITGRLTFPDAVTSPVPAVVILHGSSGVNPGEK---VW 96 F+SVTPK + V R A + G L P+ + PVPA+VI HGS+G P +K W Sbjct: 46 FNSVTPKSKWELVHRKFAKTPTPVWGTLYLPENLQGPVPAMVISHGSAG--PQQKDVDRW 103 Query: 97 AQRLNAMGYASFVVDSFTGRGVSNTEADQTQLSMSAGIADAYFALRMLAADPRIDRKKIS 156 + N MG A+FVVDSF RG++NT +Q+QL+ +A ADA FAL++L+ DPRID K+I Sbjct: 104 VRLFNQMGMAAFVVDSFGPRGIANTMDNQSQLNPAANDADALFALKLLSTDPRIDPKRIG 163 Query: 157 VMGFSRGGIAALYSALDPFRRAVIDDDLHFAAHIAFYPGCGIHY---ASAHLDGAPILML 213 +GFSRGG +L LD FR+ VIDDD FAA I FYPGC + L GAP+++ Sbjct: 164 QIGFSRGGGVSLDMTLDAFRKGVIDDDTRFAALIGFYPGCSALWWEIPPPPLTGAPLMLA 223 Query: 214 LGGKDNYTPAAPCVEYADALRKQGAQVTLKTYPDAYHGFDRPTRLHV--IKSATSARDCH 271 LG KD+YTPA C A+ +++ G V + YP AYH FD TR + AT+ARDC Sbjct: 224 LGEKDDYTPAQFCRNAAEIMQRDGQTVEMHVYPGAYHDFDN-TRAYFKYFPGATTARDCP 282 Query: 272 GTY-DLDSNQFVMRKGDQVLSGVDA-TAEARRCLSYGVMLGGDALAREQAPLDVAAFL 327 T D+ + + G + + + A A+ RC + GV G + ++A DV AFL Sbjct: 283 QTLIDVQHDAYYRLPGGEKYANLQAMQADYTRCTTRGVSTGANLEQAKRAEADVNAFL 340 >ref|YP_002983187.1| dienelactone hydrolase [Ralstonia pickettii 12D] gb|ACS64515.1| dienelactone hydrolase [Ralstonia pickettii 12D] Length = 349 Score = 206 bits (524), Expect = 5e-51, Method: Composition-based stats. Identities = 127/298 (42%), Positives = 174/298 (58%), Gaps = 14/298 (4%) Query: 41 FDSVTPKRIMDFVERAANEK-VSITGRLTFPDAVTSPVPAVVILHGSSGVNPGEK---VW 96 F+SVTPK + R ++ I G L P+ + PVPA+VI HGS+G P +K W Sbjct: 46 FNSVTPKSKWELAHRKFDKTPTPIWGTLYLPENLQGPVPAMVISHGSAG--PQQKDVDRW 103 Query: 97 AQRLNAMGYASFVVDSFTGRGVSNTEADQTQLSMSAGIADAYFALRMLAADPRIDRKKIS 156 + N MG A+FVVDSF RG++NT +Q+QL+ +A ADA FAL++L+ DPRID K+I Sbjct: 104 VRLFNQMGMAAFVVDSFGPRGIANTMDNQSQLNPAANDADALFALKLLSTDPRIDPKRIG 163 Query: 157 VMGFSRGGIAALYSALDPFRRAVIDDDLHFAAHIAFYPGCGIHY---ASAHLDGAPILML 213 +GFSRGG +L LD FR+ VI DD FAA I FYPGC + L GAP+++ Sbjct: 164 QIGFSRGGGVSLDMTLDAFRKGVIGDDTRFAALIGFYPGCSALWWEIPPPPLTGAPLMLA 223 Query: 214 LGGKDNYTPAAPCVEYADALRKQGAQVTLKTYPDAYHGFDRPTRLHV--IKSATSARDCH 271 LG KD+YTPA C A+ +++ G V + YP AYH FD TR + AT+ARDC Sbjct: 224 LGEKDDYTPAQLCRNAAEIMQRDGQTVEVHVYPGAYHDFDN-TRAYFKYFPGATTARDCP 282 Query: 272 GTY-DLDSNQFVMRKGDQVLSGVDA-TAEARRCLSYGVMLGGDALAREQAPLDVAAFL 327 T D+ + + G + + + A A+ RC + GV G + ++A DV AFL Sbjct: 283 QTLIDVQHDAYYRLPGGEKYANLQAMQADYTRCTTRGVSTGANLEQAKRAEADVNAFL 340 >ref|ZP_07675117.1| carboxymethylenebutenolidase [Ralstonia sp. 5_7_47FAA] gb|EFP66715.1| carboxymethylenebutenolidase [Ralstonia sp. 5_7_47FAA] Length = 328 Score = 205 bits (521), Expect = 1e-50, Method: Composition-based stats. Identities = 126/298 (42%), Positives = 176/298 (59%), Gaps = 14/298 (4%) Query: 41 FDSVTPKRIMDFVERAANEK-VSITGRLTFPDAVTSPVPAVVILHGSSGVNPGEK---VW 96 F+SVTPK + V R ++ + G L P+ + PVPA+VI HGS+G P +K W Sbjct: 25 FNSVTPKSKWELVHRKFDKTPTPVWGTLYLPENLQGPVPAMVISHGSAG--PQQKDVDRW 82 Query: 97 AQRLNAMGYASFVVDSFTGRGVSNTEADQTQLSMSAGIADAYFALRMLAADPRIDRKKIS 156 + N +G A+FVVDSF RG++NT +Q+QL+ +A ADA FAL++L+ DPRID K+I Sbjct: 83 VRLFNQIGMAAFVVDSFGPRGIANTMDNQSQLNPAANDADALFALKLLSTDPRIDPKRIG 142 Query: 157 VMGFSRGGIAALYSALDPFRRAVIDDDLHFAAHIAFYPGCGIHY---ASAHLDGAPILML 213 +GFSRGG +L LD FR+ VIDDD FAA I FYPGC + L GAP+++ Sbjct: 143 QIGFSRGGGVSLDMTLDAFRKGVIDDDTRFAALIGFYPGCSALWWEIPPPPLTGAPLMLA 202 Query: 214 LGGKDNYTPAAPCVEYADALRKQGAQVTLKTYPDAYHGFDRPTRLHV--IKSATSARDCH 271 LG KD+YTPA C A+ +++ G V + YP AYH FD TR + AT+ARDC Sbjct: 203 LGEKDDYTPAQLCRNAAEIMQRDGQTVEVHVYPGAYHDFDN-TRAYFKYFPGATTARDCP 261 Query: 272 GTY-DLDSNQFV-MRKGDQVLSGVDATAEARRCLSYGVMLGGDALAREQAPLDVAAFL 327 T D+ + + + G++ + A+ RC + GV G + ++A DV AFL Sbjct: 262 QTLIDVQHDAYYRLPDGEKYANLQAMQADYTRCTTRGVSTGANLEQAKRAEADVNAFL 319 >ref|YP_001206576.1| putative alpha/beta-hydrolases superfamily protein [Bradyrhizobium sp. ORS 278] emb|CAL78357.1| conserved hypothetical protein; putative alpha/beta-Hydrolases superfamily protein [Bradyrhizobium sp. ORS 278] Length = 335 Score = 163 bits (412), Expect = 4e-38, Method: Composition-based stats. Identities = 99/275 (36%), Positives = 142/275 (51%), Gaps = 13/275 (4%) Query: 61 VSITGRLTFPDAVTSPVPAVVILHGSSGVNPGEKVWAQRLNAMGYASFVVDSFTGRGVSN 120 V++ G L + +P VV++HGS GV W + NAMG ++FV+D FTGRG++ Sbjct: 65 VTVVGELRVAQG-SGRLPVVVLMHGSGGVGANIAAWTRTFNAMGISTFVIDGFTGRGITA 123 Query: 121 TEADQTQLSMSAGIADAYFALRMLAADPRIDRKKISVMGFSRGGIAALYSALDPFRRAVI 180 T +Q QL I D Y +L +LA PR+D ++I++MGFSRGG AALY++L+ F + Sbjct: 124 TSTNQAQLGRLNLIMDIYHSLDVLAKHPRVDPERIALMGFSRGGQAALYASLERFHKRWN 183 Query: 181 DDDLHFAAHIAFYPGCGIHY-ASAHLDGAPILMLLGGKDNYTPAAPCVEYADALRKQGAQ 239 L FAA+I FYP C Y + PI + GG D+Y P C Y + L+ G Sbjct: 184 TSGLQFAAYIPFYPDCSTTYRGDTDVAARPIRIFHGGPDDYNPVHTCKAYVERLKAAGRD 243 Query: 240 VTLKTYPDAYHGFD----RPTRLHVIKSATSARDCHGTYDLDSNQFVMRKGDQVLSGVDA 295 V L YPD+ HGFD L + A + R+C T + D + + Q + DA Sbjct: 244 VELTEYPDSPHGFDIGLLGANMLAIASKAQTVRNCRIT-EGDGGMLMNAETQQPFTYQDA 302 Query: 296 TAEARRCLSYGVMLGGDALAREQAPLDVAAFLKAV 330 C+ +GG+ E A V FL+A+ Sbjct: 303 ------CVELDPHVGGNPATAEAARQAVTDFLRAL 331 >ref|YP_587410.1| alpha/beta-hydrolase [Cupriavidus metallidurans CH34] gb|ABF12141.1| conserved hypothetical protein; putative alpha/beta-hydrolases superfamily protein [Cupriavidus metallidurans CH34] Length = 334 Score = 160 bits (405), Expect = 3e-37, Method: Composition-based stats. Identities = 100/276 (36%), Positives = 142/276 (51%), Gaps = 10/276 (3%) Query: 62 SITGRLTFPDAVTSPVPAVVILHGSSGVNPGEKVWAQRLNAMGYASFVVDSFTGRGVSNT 121 +I G L P A +P V++LHGS G+ W Q L MG A+FV+DSF+ RG+++T Sbjct: 58 TIAGELRLPKAGADRLPLVILLHGSGGIGSSVTDWEQDLLGMGVATFVLDSFSARGITST 117 Query: 122 EADQTQLSMSAGIADAYFALRMLAADPRIDRKKISVMGFSRGGIAALYSALDPFRRAVID 181 DQTQL A + DA+ AL +L+ PRID +I ++GFSRGG ALY++L F R Sbjct: 118 INDQTQLPRLAQMEDAFRALAILSRHPRIDPTRIMLVGFSRGGQNALYASLKRFHRLHAS 177 Query: 182 DDLHFAAHIAFYPGCGIHY-ASAHLDGAPILMLLGGKDNYTPAAPCVEYADALRKQGAQV 240 FAA++AFYP C Y L P+ +L G DNY P PC Y D L+ +G Sbjct: 178 PGTEFAAYVAFYPDCSYTYREDDDLVAKPLRILQGSADNYDPVGPCRAYVDRLKAKGNDA 237 Query: 241 TLKTYPDAYHGFDRPTRLHVIK--SATSARDCHGTYDLDSNQFVMRKGDQVLSGVDATAE 298 L YP A H FD ++ A + R C T +L++ V + + + D Sbjct: 238 MLIEYPKANHAFDSRALASAVQLPRAQTTRHCR-TMELENGLLVNAQTSKPFTYDDP--- 293 Query: 299 ARRCLSYGVMLGGDALAREQAPLDVAAFLKAVVEEG 334 C+ GV + D A +A + + AV++ G Sbjct: 294 ---CVERGVTVAYDEQAAREARQALHELVIAVLKPG 326