BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.4137.p (436 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776708.1| major facilitator superfamily permease [Herb... 718 0.0 ref|YP_999456.1| major facilitator transporter [Verminephrobacte... 557 e-156 ref|YP_296654.1| major facilitator transporter [Ralstonia eutrop... 550 e-154 ref|YP_004126274.1| major facilitator superfamily mfs_1 [Alicycl... 541 e-152 ref|YP_841272.1| major facilitator superfamily transporter AAHS ... 538 e-151 >ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] gb|ADJ64800.1| permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 414 Score = 718 bits (1853), Expect = 0.0, Method: Composition-based stats. Identities = 404/413 (97%), Positives = 408/413 (98%) Query: 23 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS 82 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS Sbjct: 1 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS 60 Query: 83 AIGGWAAGVLADRYGRVRVLQWTVLWFALFTFLSGFTTSFGELFFTRAMQGFGMGGEWAV 142 AIGGWAAGVLADRYGRVRVLQWTVLWFA FTFLSGFTTSFG+LFFTRAMQGFGMGGEWAV Sbjct: 61 AIGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAV 120 Query: 143 GSVLIAETISARHRGKASGLVQSSWAVGWAASALAFWGVYAVLPPETAWKVLFWIGVLPA 202 GSVLIAETISARHRGKASGLVQSSWAVGWA SALAFWGVYAVLPPETAWKVLFWIGVLPA Sbjct: 121 GSVLIAETISARHRGKASGLVQSSWAVGWAGSALAFWGVYAVLPPETAWKVLFWIGVLPA 180 Query: 203 LLTLYIRRAISEPEVFLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS 262 LLTLYIRRAISEP V+LATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS Sbjct: 181 LLTLYIRRAISEPAVYLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS 240 Query: 263 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV 322 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV Sbjct: 241 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV 300 Query: 323 LVLSYTQLIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV 382 LVLSYTQ+IITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV Sbjct: 301 LVLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV 360 Query: 383 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLILPETRGKELEAVVAA 435 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACL+LPETRGKELEAV A Sbjct: 361 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLVLPETRGKELEAVAKA 413 >ref|YP_999456.1| major facilitator transporter [Verminephrobacter eiseniae EF01-2] gb|ABM60438.1| major facilitator superfamily MFS_1 [Verminephrobacter eiseniae EF01-2] Length = 411 Score = 557 bits (1435), Expect = e-156, Method: Composition-based stats. Identities = 303/409 (74%), Positives = 340/409 (83%) Query: 23 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS 82 MWLQET ERRTL A F GY VD FDYMIYTF+IPTL+ W M+K EAGYIATGAL+TS Sbjct: 1 MWLQETKPQERRTLVAAFAGYGVDAFDYMIYTFMIPTLILVWGMTKTEAGYIATGALVTS 60 Query: 83 AIGGWAAGVLADRYGRVRVLQWTVLWFALFTFLSGFTTSFGELFFTRAMQGFGMGGEWAV 142 AIGGW AG+LADRYGRVR+LQ TVLWF+ FTFLSGFT S +LF TRA+QG G GGEW+V Sbjct: 61 AIGGWLAGILADRYGRVRILQLTVLWFSFFTFLSGFTQSPEQLFVTRALQGLGFGGEWSV 120 Query: 143 GSVLIAETISARHRGKASGLVQSSWAVGWAASALAFWGVYAVLPPETAWKVLFWIGVLPA 202 GSVLIAE I ARHRGKA GLVQSSWAVGW SA+AFW VYA+L P AW+VLFWIGVLPA Sbjct: 121 GSVLIAEMIQARHRGKAVGLVQSSWAVGWGVSAIAFWAVYALLEPALAWRVLFWIGVLPA 180 Query: 203 LLTLYIRRAISEPEVFLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS 262 LL LYIRR IS+PEV+ T+AR+ + G +GNFL IF LL+TT+LA LLATGMQG YY+ Sbjct: 181 LLILYIRRNISDPEVYHQTRARMAQEGDKGNFLEIFSPQLLKTTILAGLLATGMQGGYYA 240 Query: 263 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV 322 VTTWLPT+LK ER+LSV TS YLLVLI GSF GYLTSAWLSD +GRRRCFMLFA + Sbjct: 241 VTTWLPTYLKMERNLSVFNTSGYLLVLILGSFVGYLTSAWLSDKLGRRRCFMLFALCAGL 300 Query: 323 LVLSYTQLIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV 382 LV++YTQ+ ITD ML LGF LGFFLSGIFSGMGAYLSELFPS VRGSGQGFCYNFGRAV Sbjct: 301 LVIAYTQIPITDGMMLVLGFPLGFFLSGIFSGMGAYLSELFPSRVRGSGQGFCYNFGRAV 360 Query: 383 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLILPETRGKELEA 431 G+ICPAL+GY+S S+PLG IG++AAG Y LVIVA L+LPET G EL + Sbjct: 361 GSICPALIGYMSTSMPLGIAIGYMAAGGYLLVIVASLLLPETGGLELSS 409 >ref|YP_296654.1| major facilitator transporter [Ralstonia eutropha JMP134] gb|AAZ61810.1| General substrate transporter:Major facilitator superfamily MFS_1 [Ralstonia eutropha JMP134] Length = 429 Score = 550 bits (1417), Expect = e-154, Method: Composition-based stats. Identities = 288/407 (70%), Positives = 337/407 (82%) Query: 23 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS 82 MWL+ET ERRTL A F GY VD FDYMIYTF+IPT + W M+KAEAGYIATGAL++S Sbjct: 1 MWLKETNREERRTLFAAFVGYGVDAFDYMIYTFMIPTFILVWGMTKAEAGYIATGALISS 60 Query: 83 AIGGWAAGVLADRYGRVRVLQWTVLWFALFTFLSGFTTSFGELFFTRAMQGFGMGGEWAV 142 AIGGW AG+LAD+YGRVR+LQ TVLWF+LFTFLSGFT S +LF TR +QG G GGEW+V Sbjct: 61 AIGGWLAGILADKYGRVRILQLTVLWFSLFTFLSGFTQSPDQLFITRMLQGLGFGGEWSV 120 Query: 143 GSVLIAETISARHRGKASGLVQSSWAVGWAASALAFWGVYAVLPPETAWKVLFWIGVLPA 202 GSVLIAE I ARHRGKA GLVQSSWAVGW SA+AFW VYA++ + AW+VLFW+G LPA Sbjct: 121 GSVLIAEMIRARHRGKAVGLVQSSWAVGWGISAIAFWAVYALIDQQHAWRVLFWLGALPA 180 Query: 203 LLTLYIRRAISEPEVFLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS 262 L LYIRR ISEPEV+ T+A+L +G+ +F+ IF +L TL+ASLLATGMQGAYYS Sbjct: 181 LFILYIRRNISEPEVYRETKAKLARTGESNSFMLIFKPGVLGVTLMASLLATGMQGAYYS 240 Query: 263 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV 322 VTTWLPT+LK ERHLSVL TS YL+VLI GSF GYLTSAWLSD +GR+RCFMLFA V Sbjct: 241 VTTWLPTYLKMERHLSVLNTSGYLMVLIAGSFAGYLTSAWLSDQLGRKRCFMLFAVSAAV 300 Query: 323 LVLSYTQLIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV 382 LV YTQL ITD M+ LGF LGFFLSGIFSGMGAYL+ELFPS +RGSGQGFCYNFGRAV Sbjct: 301 LVTCYTQLPITDGAMMVLGFPLGFFLSGIFSGMGAYLTELFPSHIRGSGQGFCYNFGRAV 360 Query: 383 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLILPETRGKEL 429 GA+ PA++G++S S+PLG IG++AAG Y LV++ACL+LPET+G++L Sbjct: 361 GAVFPAMIGHMSTSMPLGTAIGYLAAGAYTLVVIACLLLPETQGRDL 407 >ref|YP_004126274.1| major facilitator superfamily mfs_1 [Alicycliphilus denitrificans BC] ref|YP_004389045.1| general substrate transporter [Alicycliphilus denitrificans K601] gb|ADU99386.1| major facilitator superfamily MFS_1 [Alicycliphilus denitrificans BC] gb|AEB85529.1| General substrate transporter [Alicycliphilus denitrificans K601] Length = 415 Score = 541 bits (1395), Expect = e-152, Method: Composition-based stats. Identities = 291/407 (71%), Positives = 339/407 (83%) Query: 23 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS 82 MWL+ET+ ER+TL A F GY VD FDYMIYTF+IPT + W M+K EAGYIATGAL++S Sbjct: 1 MWLKETSQEERKTLFAAFVGYGVDAFDYMIYTFMIPTFILVWGMTKTEAGYIATGALISS 60 Query: 83 AIGGWAAGVLADRYGRVRVLQWTVLWFALFTFLSGFTTSFGELFFTRAMQGFGMGGEWAV 142 AIGGW AG+LAD+YGRVR+LQ TVLWF+ FTFLSGFT S +LF TRA+QG G GGEW+V Sbjct: 61 AIGGWLAGILADKYGRVRILQLTVLWFSFFTFLSGFTHSPEQLFVTRALQGLGFGGEWSV 120 Query: 143 GSVLIAETISARHRGKASGLVQSSWAVGWAASALAFWGVYAVLPPETAWKVLFWIGVLPA 202 GSVLIAE I ARHRGKA GLVQSSWAVGW SA+AFW VYA++ +TAW+VLFW+GVLPA Sbjct: 121 GSVLIAEMIRARHRGKAVGLVQSSWAVGWGLSAIAFWAVYALVEQQTAWRVLFWLGVLPA 180 Query: 203 LLTLYIRRAISEPEVFLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS 262 L LYIRR ISEP VF ATQ RL ++GQ +F+ IF +LR T+ ASLLATGMQGAYYS Sbjct: 181 LFILYIRRNISEPTVFRATQERLAKTGQSSHFMLIFKPGVLRVTVWASLLATGMQGAYYS 240 Query: 263 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV 322 VTTWLPT+LK ER+LSVL TS YL+VLI GSF GYLTSAWLSD +GRRRCFMLFA V Sbjct: 241 VTTWLPTYLKMERNLSVLNTSGYLMVLIAGSFAGYLTSAWLSDRLGRRRCFMLFAVSAAV 300 Query: 323 LVLSYTQLIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV 382 LV YTQL ITDA M+ LGF LGFFLSGIFSGMGAYL+EL+PS +RGSGQGFCYNFGRAV Sbjct: 301 LVTFYTQLPITDAAMMVLGFPLGFFLSGIFSGMGAYLTELYPSHIRGSGQGFCYNFGRAV 360 Query: 383 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLILPETRGKEL 429 G++CPA+VGY+S+++ LG IG++A G Y LV++ACL LPET+G+EL Sbjct: 361 GSVCPAMVGYMSSTMSLGVAIGYMAVGAYALVVIACLALPETQGREL 407 >ref|YP_841272.1| major facilitator superfamily transporter AAHS family protein [Ralstonia eutropha H16] emb|CAJ96542.1| MFS transporter, AAHS family [Ralstonia eutropha H16] Length = 417 Score = 538 bits (1386), Expect = e-151, Method: Composition-based stats. Identities = 287/405 (70%), Positives = 336/405 (82%) Query: 23 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS 82 MWL+ET +ER+TL A F GY VD FDYMIYTF+IPT + W M+KAEAGYIATGAL++S Sbjct: 1 MWLKETNQAERKTLFAAFVGYGVDAFDYMIYTFMIPTFILVWGMTKAEAGYIATGALISS 60 Query: 83 AIGGWAAGVLADRYGRVRVLQWTVLWFALFTFLSGFTTSFGELFFTRAMQGFGMGGEWAV 142 AIGGW AG+LAD+YGRVR+LQ TVLWF+ FTFLSGFT S +LF TR +QG G GGEW+V Sbjct: 61 AIGGWLAGILADKYGRVRILQLTVLWFSFFTFLSGFTQSPEQLFLTRMLQGLGFGGEWSV 120 Query: 143 GSVLIAETISARHRGKASGLVQSSWAVGWAASALAFWGVYAVLPPETAWKVLFWIGVLPA 202 GS+LIAE I ARHRGKA GLVQSSWA+GW SA+AFW VYAV + AW+VLFW GVLPA Sbjct: 121 GSLLIAEMIRARHRGKAVGLVQSSWAIGWGLSAIAFWAVYAVFEQQYAWRVLFWAGVLPA 180 Query: 203 LLTLYIRRAISEPEVFLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS 262 + LYIRR ISEPEV+ T+A+L +GQ NF+ IF +LR T+LASLLATGMQGAYYS Sbjct: 181 VFILYIRRNISEPEVYQETRAKLARTGQSNNFMLIFKPGVLRVTVLASLLATGMQGAYYS 240 Query: 263 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV 322 VTTWLPT+LK ER+LSVL TS YL+VLI GSF GYLTSAWLSD +GRRRCFMLFA Sbjct: 241 VTTWLPTYLKMERNLSVLNTSGYLMVLIAGSFAGYLTSAWLSDRLGRRRCFMLFAVSAAF 300 Query: 323 LVLSYTQLIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV 382 LV+ YTQL ITDA ML LGF LGFFLSGIFSGMGAYL+EL+PS +RGSGQGFCYNFGRAV Sbjct: 301 LVICYTQLPITDAAMLVLGFPLGFFLSGIFSGMGAYLTELYPSHIRGSGQGFCYNFGRAV 360 Query: 383 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLILPETRGK 427 G+I PA++G++S S+ LG IG++AAG YGLV++ACL+LPET+G+ Sbjct: 361 GSIFPAMIGHMSASMSLGVAIGYLAAGAYGLVVIACLLLPETQGR 405