BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.3835.p (372 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776585.1| fatty acid desaturase [Herbaspirillum serope... 673 0.0 ref|YP_725522.1| fatty acid desaturase [Ralstonia eutropha H16] ... 488 e-136 ref|YP_004684793.1| fatty acid desaturase [Cupriavidus necator N... 487 e-135 ref|YP_002005028.1| fatty acid desaturase, family 2 [Cupriavidus... 484 e-135 ref|YP_296636.1| Fatty acid desaturase [Ralstonia eutropha JMP13... 481 e-134 >ref|YP_003776585.1| fatty acid desaturase [Herbaspirillum seropedicae SmR1] gb|ADJ64677.1| fatty acid desaturase protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 673 bits (1736), Expect = 0.0, Method: Composition-based stats. Identities = 345/372 (92%), Positives = 357/372 (95%), Gaps = 1/372 (0%) Query: 1 MSTTSHP-NTPVAPGAPMPHRKIIRSWVIPISQRSTAIALALSVLDFCLFGASLAAIVWA 59 MSTTS N PVAPGAPMPHRK+IRSWVIP+S+RSTAIAL LSV+DF LFGASLAAIVWA Sbjct: 18 MSTTSQSHNLPVAPGAPMPHRKVIRSWVIPLSKRSTAIALLLSVIDFALFGASLAAIVWA 77 Query: 60 PWLLAKIALGVVNGFIIGRLFILGHDACHQAFTPSRKLNTWLGRLLFMPSLTPYSLWAVG 119 PWLLAKIALGVVNGFIIGRLFILGHDACHQAFTP+RKLNTWLGRLLFMPSLTPYSLWAVG Sbjct: 78 PWLLAKIALGVVNGFIIGRLFILGHDACHQAFTPNRKLNTWLGRLLFMPSLTPYSLWAVG 137 Query: 120 HNVVHHGYTNLKGFDFVWQPRSLEEFRALPRWRQRLERIYRSGFGPGLYYMIDMWWKRMY 179 HNVVHHGYTNLKGFDFVWQPRSLEEF ALPRWRQRLERIYRSGFGPGLYYMIDMWWKRMY Sbjct: 138 HNVVHHGYTNLKGFDFVWQPRSLEEFNALPRWRQRLERIYRSGFGPGLYYMIDMWWKRMY 197 Query: 180 FPSKKQMPTRRAEFMWDGVLVTAVAVVWIAGLAWAAQATEQSFWTLLVTGFAIPLLFWKA 239 FPSKKQMPTRRAEFMWDG LVTAVA VW+ GLAWAAQ+T QSFWTLLVTGF IPLLFWKA Sbjct: 198 FPSKKQMPTRRAEFMWDGALVTAVAAVWVGGLAWAAQSTGQSFWTLLVTGFVIPLLFWKA 257 Query: 240 MIGFVVYVHHTHTSVAWYEDKITWAAAQPFVSTTVHLTFGRYWGSLLHHIMEHTAHHVDM 299 MIGFVVYVHHTHTSVAWY+DK+TWAAAQPFVSTTVHLTFGR+WG+LLHHIMEHTAHHVDM Sbjct: 258 MIGFVVYVHHTHTSVAWYDDKVTWAAAQPFVSTTVHLTFGRFWGALLHHIMEHTAHHVDM 317 Query: 300 SVPLYRLKQAQKKLETMLPGRIVIQRFSWRWYFDTARRCKLYDFKRLCWTDYRGRPTSTP 359 SVPLYRLKQAQKKLETMLPGRIVIQRFSWRWYFDTARRCKLYDFKRLCWTDYRGRPTS P Sbjct: 318 SVPLYRLKQAQKKLETMLPGRIVIQRFSWRWYFDTARRCKLYDFKRLCWTDYRGRPTSQP 377 Query: 360 LPLPDMSKPTAE 371 LPLP+MSK AE Sbjct: 378 LPLPEMSKSMAE 389 >ref|YP_725522.1| fatty acid desaturase [Ralstonia eutropha H16] emb|CAJ92154.1| fatty acid desaturase [Ralstonia eutropha H16] Length = 391 Score = 488 bits (1255), Expect = e-136, Method: Composition-based stats. Identities = 244/354 (68%), Positives = 276/354 (77%) Query: 10 PVAPGAPMPHRKIIRSWVIPISQRSTAIALALSVLDFCLFGASLAAIVWAPWLLAKIALG 69 P+AP AP+P RKIIR W+IP+ QRST AL L D+ LFGA LA +V A AK+ALG Sbjct: 37 PIAPDAPLPSRKIIRGWLIPLGQRSTPRALLLFAFDYLLFGAVLAGVVLAQHWAAKLALG 96 Query: 70 VVNGFIIGRLFILGHDACHQAFTPSRKLNTWLGRLLFMPSLTPYSLWAVGHNVVHHGYTN 129 V+ G II RLFI+GHDACHQ+ TP R LN WLGRL F+PSLTPYSLW VGHNVVHHGYTN Sbjct: 97 VLAGLIIARLFIIGHDACHQSLTPRRGLNKWLGRLTFLPSLTPYSLWEVGHNVVHHGYTN 156 Query: 130 LKGFDFVWQPRSLEEFRALPRWRQRLERIYRSGFGPGLYYMIDMWWKRMYFPSKKQMPTR 189 LKGFDFVW P SLEEF ALPRWR+ +ERIYR+GFGPGLYY++++WW +M+FPSK+QM TR Sbjct: 157 LKGFDFVWAPYSLEEFNALPRWRRVMERIYRTGFGPGLYYLVEIWWCKMFFPSKRQMATR 216 Query: 190 RAEFMWDGVLVTAVAVVWIAGLAWAAQATEQSFWTLLVTGFAIPLLFWKAMIGFVVYVHH 249 R F D VLV A + WI LA A ATEQS W L GF +P L W +GFV+YVHH Sbjct: 217 RPIFTGDCVLVAAFGLAWIGALAGLALATEQSVWLLTGAGFVLPFLVWNVTVGFVLYVHH 276 Query: 250 THTSVAWYEDKITWAAAQPFVSTTVHLTFGRYWGSLLHHIMEHTAHHVDMSVPLYRLKQA 309 THTSVAWY+ K WA AQPFVSTTVHL F G+ LHHIMEHTAHHVDMSVPLYRLK+A Sbjct: 277 THTSVAWYDTKAMWAKAQPFVSTTVHLRFRHGIGAALHHIMEHTAHHVDMSVPLYRLKRA 336 Query: 310 QKKLETMLPGRIVIQRFSWRWYFDTARRCKLYDFKRLCWTDYRGRPTSTPLPLP 363 Q LE LPGRI+I+ FSWRWYFDTARRCKLYDFK LCWTD+RGR TS P+P Sbjct: 337 QALLEHALPGRIIIENFSWRWYFDTARRCKLYDFKALCWTDFRGRQTSDNAPVP 390 >ref|YP_004684793.1| fatty acid desaturase [Cupriavidus necator N-1] gb|AEI76312.1| fatty acid desaturase [Cupriavidus necator N-1] Length = 391 Score = 487 bits (1253), Expect = e-135, Method: Composition-based stats. Identities = 243/354 (68%), Positives = 277/354 (78%) Query: 10 PVAPGAPMPHRKIIRSWVIPISQRSTAIALALSVLDFCLFGASLAAIVWAPWLLAKIALG 69 P+AP AP+P RKIIR W+IP+ QRST AL L D+ LFGA LA +V A AK+ALG Sbjct: 37 PIAPDAPLPSRKIIRGWLIPLGQRSTPRALLLFAFDYLLFGAVLAGVVLAQHWAAKLALG 96 Query: 70 VVNGFIIGRLFILGHDACHQAFTPSRKLNTWLGRLLFMPSLTPYSLWAVGHNVVHHGYTN 129 V+ G II RLFI+GHDACHQ+ TP R LN WLGRL F+PSLTPYSLW VGHNVVHHGYTN Sbjct: 97 VLAGLIIARLFIIGHDACHQSLTPRRGLNKWLGRLTFLPSLTPYSLWEVGHNVVHHGYTN 156 Query: 130 LKGFDFVWQPRSLEEFRALPRWRQRLERIYRSGFGPGLYYMIDMWWKRMYFPSKKQMPTR 189 LKGFDFVW P SLEEF ALPRWR+ +ERIYR+GFGPGLYY++++WW +M+FPSK+QM TR Sbjct: 157 LKGFDFVWAPYSLEEFNALPRWRRVMERIYRTGFGPGLYYLVEIWWCKMFFPSKRQMATR 216 Query: 190 RAEFMWDGVLVTAVAVVWIAGLAWAAQATEQSFWTLLVTGFAIPLLFWKAMIGFVVYVHH 249 R F D VLV A + WI LA A AT+QS W L+ GF +P L W +GFV+YVHH Sbjct: 217 RPIFTGDCVLVAAFGLAWIGALAGLALATDQSVWMLIGAGFVLPFLVWNVTVGFVLYVHH 276 Query: 250 THTSVAWYEDKITWAAAQPFVSTTVHLTFGRYWGSLLHHIMEHTAHHVDMSVPLYRLKQA 309 THTSVAWY+ K WA AQPFVSTTVHL F G+ LHHIMEHTAHHVDMSVPLYRLK+A Sbjct: 277 THTSVAWYDTKAMWAKAQPFVSTTVHLRFRHGIGAALHHIMEHTAHHVDMSVPLYRLKRA 336 Query: 310 QKKLETMLPGRIVIQRFSWRWYFDTARRCKLYDFKRLCWTDYRGRPTSTPLPLP 363 Q LE LPGRI+I+ FSWRWYFDTARRCKLYDFK LCWTD+RGR TS P+P Sbjct: 337 QALLEHALPGRIIIENFSWRWYFDTARRCKLYDFKALCWTDFRGRQTSDNAPVP 390 >ref|YP_002005028.1| fatty acid desaturase, family 2 [Cupriavidus taiwanensis LMG 19424] emb|CAQ68961.1| putative Fatty acid desaturase, family 2 [Cupriavidus taiwanensis LMG 19424] Length = 387 Score = 484 bits (1246), Expect = e-135, Method: Composition-based stats. Identities = 241/354 (68%), Positives = 277/354 (78%) Query: 10 PVAPGAPMPHRKIIRSWVIPISQRSTAIALALSVLDFCLFGASLAAIVWAPWLLAKIALG 69 P+AP AP+P RKIIR W+IP+ QRST AL L D+ LFGA LA +V AP AK+ALG Sbjct: 33 PIAPDAPLPSRKIIRGWLIPLGQRSTPRALMLFAFDYLLFGAVLAGVVLAPHWAAKLALG 92 Query: 70 VVNGFIIGRLFILGHDACHQAFTPSRKLNTWLGRLLFMPSLTPYSLWAVGHNVVHHGYTN 129 V+ G +I RLFI+GHDACHQ+ TP R LN WLGRL F+PSLTPYSLW VGHNVVHHGYTN Sbjct: 93 VLAGLVIARLFIIGHDACHQSLTPRRGLNKWLGRLTFLPSLTPYSLWEVGHNVVHHGYTN 152 Query: 130 LKGFDFVWQPRSLEEFRALPRWRQRLERIYRSGFGPGLYYMIDMWWKRMYFPSKKQMPTR 189 LKGFDFVW P SLEEF ALPRWR+ +ERIYR+GFGPGLYY++++WW +++FPSK+QM TR Sbjct: 153 LKGFDFVWAPYSLEEFNALPRWRRVMERIYRTGFGPGLYYLVEIWWCKLFFPSKRQMATR 212 Query: 190 RAEFMWDGVLVTAVAVVWIAGLAWAAQATEQSFWTLLVTGFAIPLLFWKAMIGFVVYVHH 249 R F D VLV + WI LA A AT+QS W L+ GF +P L W +GFV+YVHH Sbjct: 213 RPVFTGDCVLVAVFGLAWIGALAGLALATQQSVWMLVGAGFVLPFLVWNVTVGFVLYVHH 272 Query: 250 THTSVAWYEDKITWAAAQPFVSTTVHLTFGRYWGSLLHHIMEHTAHHVDMSVPLYRLKQA 309 THTSVAWY+ K WA AQPFVSTTVHL F G+ LHHIMEHTAHHVDMSVPLYRLK+A Sbjct: 273 THTSVAWYDTKAMWARAQPFVSTTVHLRFRHGIGTALHHIMEHTAHHVDMSVPLYRLKRA 332 Query: 310 QKKLETMLPGRIVIQRFSWRWYFDTARRCKLYDFKRLCWTDYRGRPTSTPLPLP 363 Q LE LPGRI+I+ FSWRWYFDTARRCKLYDFK LCWTD+RGR TS P+P Sbjct: 333 QALLEHALPGRIIIENFSWRWYFDTARRCKLYDFKALCWTDFRGRQTSENAPVP 386 >ref|YP_296636.1| Fatty acid desaturase [Ralstonia eutropha JMP134] gb|AAZ61792.1| Fatty acid desaturase [Ralstonia eutropha JMP134] Length = 380 Score = 481 bits (1238), Expect = e-134, Method: Composition-based stats. Identities = 239/354 (67%), Positives = 276/354 (77%) Query: 10 PVAPGAPMPHRKIIRSWVIPISQRSTAIALALSVLDFCLFGASLAAIVWAPWLLAKIALG 69 P++P P+P RKIIR W+IP+ QRST ALAL D+ LF A+LA +V AP AK+ LG Sbjct: 26 PISPDQPLPSRKIIRGWLIPLGQRSTPRALALFAFDYLLFAATLAGVVLAPHWAAKVGLG 85 Query: 70 VVNGFIIGRLFILGHDACHQAFTPSRKLNTWLGRLLFMPSLTPYSLWAVGHNVVHHGYTN 129 VV G II RLFI+GHDACHQ+ TP R LN WLGRL F+PSLTPYSLW VGHNVVHHGYTN Sbjct: 86 VVAGLIIARLFIIGHDACHQSLTPRRGLNKWLGRLTFLPSLTPYSLWEVGHNVVHHGYTN 145 Query: 130 LKGFDFVWQPRSLEEFRALPRWRQRLERIYRSGFGPGLYYMIDMWWKRMYFPSKKQMPTR 189 LKGFDFVW P SLEEF+ALPRWR+ +ERIYR+GFGPGLYY++++WW +++FP+K+QM TR Sbjct: 146 LKGFDFVWAPYSLEEFQALPRWRRVMERIYRTGFGPGLYYLVEIWWCKLFFPNKRQMATR 205 Query: 190 RAEFMWDGVLVTAVAVVWIAGLAWAAQATEQSFWTLLVTGFAIPLLFWKAMIGFVVYVHH 249 R FM D LV WI L WAA AT QS W ++ GF +P L W +GFV+YVHH Sbjct: 206 RPIFMADCTLVATFGAAWIGALIWAAFATGQSAWMMVGAGFVLPFLVWNVTVGFVLYVHH 265 Query: 250 THTSVAWYEDKITWAAAQPFVSTTVHLTFGRYWGSLLHHIMEHTAHHVDMSVPLYRLKQA 309 THTSVAWY+ K WA AQPFVSTTVHL F G+ LHHIMEHTAHHVDMSVPLYRLK+A Sbjct: 266 THTSVAWYDTKSMWAKAQPFVSTTVHLRFRHGIGAALHHIMEHTAHHVDMSVPLYRLKRA 325 Query: 310 QKKLETMLPGRIVIQRFSWRWYFDTARRCKLYDFKRLCWTDYRGRPTSTPLPLP 363 Q LE LPGRI+I+ FSWRWYFDTARRCKLYDFK LCWTD+RGR TS P+P Sbjct: 326 QALLEHALPGRIIIENFSWRWYFDTARRCKLYDFKALCWTDFRGRQTSEQSPVP 379