BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.3647.p (345 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08503164.1| hypothetical protein METUNv1_00154 [Methylove... 413 e-113 ref|YP_297505.1| putative transmembrane protein [Ralstonia eutro... 392 e-107 ref|ZP_00944497.1| Hypothetical membrane spanning protein [Ralst... 388 e-106 ref|YP_585600.1| hypothetical protein Rmet_3459 [Cupriavidus met... 388 e-106 emb|CAQ37155.1| conserved hypothetical protein [Ralstonia solana... 384 e-105 >ref|ZP_08503164.1| hypothetical protein METUNv1_00154 [Methyloversatilis universalis FAM5] gb|EGK73526.1| hypothetical protein METUNv1_00154 [Methyloversatilis universalis FAM5] Length = 339 Score = 413 bits (1061), Expect = e-113, Method: Composition-based stats. Identities = 214/345 (62%), Positives = 269/345 (77%), Gaps = 7/345 (2%) Query: 1 MNLGSFIKKQFIDVLQWNEDQEGVLAWRYPMQDFEIQNGATLTVRESQLAVFVNEGQVAD 60 M +G FIKKQFIDVLQWNED +GVLAWRYPMQDFEIQ GA LTVRESQ+AVFVNEG+VAD Sbjct: 1 MGIGDFIKKQFIDVLQWNEDGDGVLAWRYPMQDFEIQYGAKLTVRESQMAVFVNEGKVAD 60 Query: 61 IFTPGNYTLTTRTLPVLTNLKNWDKLFESPFKSDVYFFSTRLQTGRKWGTPQPITIRDKD 120 +F PG +TL T TLPVLT L+NWDKLFESPFKSDVYFFSTRLQ GRKWGTPQP+T+RD+D Sbjct: 61 VFGPGLHTLNTNTLPVLTYLQNWDKLFESPFKSDVYFFSTRLQLGRKWGTPQPVTVRDED 120 Query: 121 FDMIRVRAFGMYSYALSDPRVFFSRISGTREMYTREDFEDQLQGILLSTLANSLGNAQSA 180 F M+R+RAFGMYSY L+DP+ FF+ ISGTR+ Y+ +D E QL+ +++ST++++LGN+ + Sbjct: 121 FGMVRLRAFGMYSYKLADPKKFFTEISGTRDTYSVDDLEMQLRNLVVSTMSSTLGNSAAP 180 Query: 181 FLDLAANQTLMANTVRDAMALSFAQYGLTLDQFNIASFNLPDDLQVALDQRIAAGMKGGL 240 FLD+AAN +M++ V+ AM F +YG+ +D F + S +LP++LQ ALD RI+ GM G + Sbjct: 181 FLDMAANLGVMSDAVKTAMTPVFERYGVAIDNFAVESISLPEELQKALDTRISMGMVGNM 240 Query: 241 DAAKAAGYARVQAAEAIPIAAGNEGGLAGLGAGLATGVSMGQVMADALGTGAAGAQGHAA 300 A Y + Q A AIP+AA NEGGLAG+GA L G++MGQVM +A+ AG A Sbjct: 241 -----AAYTQYQTANAIPLAAQNEGGLAGIGASLGAGLTMGQVMTEAMRAAQAGTPAAAP 295 Query: 301 AAPAAAAGPTPAERLGQLKQLLDQGLISAADYDAAKAEVLKKLIG 345 AA A TP RLG+LK LLD GLI+ ADYDAAKA+V+K++ G Sbjct: 296 AAAPAV--DTPEARLGKLKSLLDGGLITQADYDAAKADVIKQITG 338 >ref|YP_297505.1| putative transmembrane protein [Ralstonia eutropha JMP134] gb|AAZ62661.1| putative transmembrane protein [Ralstonia eutropha JMP134] Length = 344 Score = 392 bits (1006), Expect = e-107, Method: Composition-based stats. Identities = 209/349 (59%), Positives = 264/349 (75%), Gaps = 9/349 (2%) Query: 1 MNLGSFIKKQFIDVLQWNEDQEGVLAWRYPMQDFEIQNGATLTVRESQLAVFVNEGQVAD 60 M++GSFIKKQFID+LQW ED +GVLAWRYPM+D EIQ G +LTVRESQ+AVFVNEG++AD Sbjct: 1 MSIGSFIKKQFIDILQWTEDTDGVLAWRYPMEDMEIQYGGSLTVRESQMAVFVNEGKIAD 60 Query: 61 IFTPGNYTLTTRTLPVLTNLKNWDKLFESPFKSDVYFFSTRLQTGRKWGTPQPITIRDKD 120 +F PG Y LTT+TLPVLT LKNWDKLFESPFKSDVYFFSTRLQ GRKWGTPQPITIRD D Sbjct: 61 VFGPGMYKLTTQTLPVLTYLKNWDKLFESPFKSDVYFFSTRLQIGRKWGTPQPITIRDAD 120 Query: 121 FDMIRVRAFGMYSYALSDPRVFFSRISGTREMYTREDFEDQLQGILLSTLANSLGNAQSA 180 F M+R+RAFG+YSY ++D F+S ISGTR YTR++ E+QL+ +L++T+ N+LG + Sbjct: 121 FGMVRLRAFGLYSYKVADAAKFYSEISGTRAEYTRDEVEEQLRNLLIATMTNALGASSLP 180 Query: 181 FLDLAANQTLMANTVRDAMALSFAQYGLTLDQFNIASFNLPDDLQVALDQRIAAGMKGGL 240 FLD+AANQ LM+ T+R+ +A F +YG+ LD F + + +LP++LQ A+D RI+ GM G + Sbjct: 181 FLDMAANQALMSQTIRERLAPEFERYGVALDNFAVTNVSLPEELQKAIDTRISMGMMGDM 240 Query: 241 DAAKAAGYARVQAAEAIPIAAGNEGGLAGLGAGLATGVSMGQVMADALGTGAAGAQGHAA 300 A Y + Q A +IP+AA NEGG+AGLGA LA G MGQ MA L A A Sbjct: 241 -----AKYTQYQVASSIPLAAQNEGGVAGLGASLAAGAVMGQAMAGGLAGAAGAPGVPAP 295 Query: 301 AAPAAAAGPT----PAERLGQLKQLLDQGLISAADYDAAKAEVLKKLIG 345 A P AA P RL +LK+LLD+ LI+ A++D+AKAE+LKKL+G Sbjct: 296 AVPPAAPQAAAADDPTARLQKLKELLDKSLITQAEFDSAKAEILKKLVG 344 >ref|ZP_00944497.1| Hypothetical membrane spanning protein [Ralstonia solanacearum UW551] gb|EAP73054.1| Hypothetical membrane spanning protein [Ralstonia solanacearum UW551] Length = 488 Score = 388 bits (997), Expect = e-106, Method: Composition-based stats. Identities = 208/349 (59%), Positives = 269/349 (77%), Gaps = 12/349 (3%) Query: 1 MNLGSFIKKQFIDVLQWNEDQEGVLAWRYPMQDFEIQNGATLTVRESQLAVFVNEGQVAD 60 ++L SFIKKQFID+LQW E ++GVLAWRYPMQD EIQNG +LTVR+SQ+A+FVNEGQ+AD Sbjct: 145 VSLSSFIKKQFIDILQWTESEDGVLAWRYPMQDMEIQNGGSLTVRDSQMAMFVNEGQIAD 204 Query: 61 IFTPGNYTLTTRTLPVLTNLKNWDKLFESPFKSDVYFFSTRLQTGRKWGTPQPITIRDKD 120 +F PG + LTT+TLPVLT LKNWDKLFESPFKSDVYFFSTR Q GR+WGTPQP+T+RDKD Sbjct: 205 VFRPGMFKLTTQTLPVLTYLKNWDKLFESPFKSDVYFFSTRQQLGRRWGTPQPVTVRDKD 264 Query: 121 FDMIRVRAFGMYSYALSDPRVFFSRISGTREMYTREDFEDQLQGILLSTLANSLGNAQSA 180 F M+R+RAFG+Y+Y ++DP++F+ ++SGTR++YT +D E QL +++ +A + G + Sbjct: 265 FGMVRLRAFGVYAYHVTDPKLFYQQVSGTRDLYTVDDMEQQLGPVIMGAMATAFGESGVP 324 Query: 181 FLDLAANQTLMANTVRDAMALSFAQYGLTLDQFNIASFNLPDDLQVALDQRIAAGMKGGL 240 F+DLAANQ L++N VR+A+ F QYGL LD F ++S LPD+LQ ALD+RI+ M G + Sbjct: 325 FVDLAANQALLSNKVREALLPQFTQYGLALDSFQVSSVTLPDELQAALDRRISMDMTGDM 384 Query: 241 DAAKAAGYARVQAAEAIPIAAGNEGGLAGLGAGLATGVSMGQVMADALGTGAAGAQGHAA 300 + + Q AE++P+AA NEGG+AG GAGLA G++MGQ MAD+L T QGHA Sbjct: 385 QR-----FTQYQTAESLPLAARNEGGIAGTGAGLAAGLAMGQAMADSLRT---AVQGHAG 436 Query: 301 AA----PAAAAGPTPAERLGQLKQLLDQGLISAADYDAAKAEVLKKLIG 345 AA PAA A P RL +LK+LLD+GLI+ AD+DA KA VLK L G Sbjct: 437 AAPVVQPAAPAVDDPTARLAKLKELLDKGLITQADFDAGKAAVLKALTG 485 >ref|YP_585600.1| hypothetical protein Rmet_3459 [Cupriavidus metallidurans CH34] gb|ABF10331.1| conserved hypothetical protein, putative transmembrane protein [Cupriavidus metallidurans CH34] Length = 340 Score = 388 bits (997), Expect = e-106, Method: Composition-based stats. Identities = 213/345 (61%), Positives = 268/345 (77%), Gaps = 5/345 (1%) Query: 1 MNLGSFIKKQFIDVLQWNEDQEGVLAWRYPMQDFEIQNGATLTVRESQLAVFVNEGQVAD 60 M++GSFI+KQFID+LQW ED +GVLAWRYPM+D EIQ G +LTVRESQ+AVFVNEG++AD Sbjct: 1 MSIGSFIRKQFIDILQWTEDTDGVLAWRYPMEDMEIQYGGSLTVRESQMAVFVNEGKIAD 60 Query: 61 IFTPGNYTLTTRTLPVLTNLKNWDKLFESPFKSDVYFFSTRLQTGRKWGTPQPITIRDKD 120 +F PG Y LTT+TLPVLT LKNWDKLFESPFKSDVYFFSTRLQ GRKWGT QPITIRD D Sbjct: 61 VFGPGMYKLTTQTLPVLTYLKNWDKLFESPFKSDVYFFSTRLQIGRKWGTAQPITIRDAD 120 Query: 121 FDMIRVRAFGMYSYALSDPRVFFSRISGTREMYTREDFEDQLQGILLSTLANSLGNAQSA 180 F M+R+RAFG+YSY +SDP+ F++ ISGTR YTR++ E+QL+ +L++T+ N+LG + Sbjct: 121 FGMVRLRAFGLYSYKISDPQKFYTEISGTRAEYTRDEVEEQLRNLLIATMTNTLGASSVP 180 Query: 181 FLDLAANQTLMANTVRDAMALSFAQYGLTLDQFNIASFNLPDDLQVALDQRIAAGMKGGL 240 FLD+AANQTLM+ T+ + +A F +YG+ LD F + + +LP++LQ A+D RI+ GM G + Sbjct: 181 FLDMAANQTLMSQTIHEKLAPEFERYGVALDNFAVTNVSLPEELQKAIDTRISMGMMGDM 240 Query: 241 DAAKAAGYARVQAAEAIPIAAGNEGGLAGLGAGLATGVSMGQVMADALGTGAAGAQGHAA 300 A Y + Q A +IP+AA NEGG+AGLGAGLA G MGQ MA L G AG A Sbjct: 241 -----AKYTQYQVASSIPLAAQNEGGVAGLGAGLAAGAVMGQAMAGGLVGGVAGVPAAAP 295 Query: 301 AAPAAAAGPTPAERLGQLKQLLDQGLISAADYDAAKAEVLKKLIG 345 APA A PA RL +LK+LLD+ LIS A++D AKAE+LKKL+G Sbjct: 296 VAPATPAAEDPAARLQKLKELLDKSLISQAEFDTAKAEILKKLVG 340 >emb|CAQ37155.1| conserved hypothetical protein [Ralstonia solanacearum MolK2] Length = 342 Score = 384 bits (987), Expect = e-105, Method: Composition-based stats. Identities = 208/347 (59%), Positives = 267/347 (76%), Gaps = 12/347 (3%) Query: 3 LGSFIKKQFIDVLQWNEDQEGVLAWRYPMQDFEIQNGATLTVRESQLAVFVNEGQVADIF 62 + SFIKKQFID+LQW E ++GVLAWRYPMQD EIQNG +LTVR+SQ+A+FVNEGQ+AD+F Sbjct: 1 MSSFIKKQFIDILQWTESEDGVLAWRYPMQDMEIQNGGSLTVRDSQMAMFVNEGQIADVF 60 Query: 63 TPGNYTLTTRTLPVLTNLKNWDKLFESPFKSDVYFFSTRLQTGRKWGTPQPITIRDKDFD 122 PG + LTT+TLPVLT LKNWDKLFESPFKSDVYFFSTR Q GR+WGTPQP+T+RDKDF Sbjct: 61 RPGMFKLTTQTLPVLTYLKNWDKLFESPFKSDVYFFSTRQQLGRRWGTPQPVTVRDKDFG 120 Query: 123 MIRVRAFGMYSYALSDPRVFFSRISGTREMYTREDFEDQLQGILLSTLANSLGNAQSAFL 182 M+R+RAFG+Y+Y ++DP++F+ ++SGTR++YT ED E QL +++ +A + G + F+ Sbjct: 121 MVRLRAFGVYAYHVTDPKLFYQQVSGTRDLYTVEDMEQQLGPVIMGAMATAFGESGVPFV 180 Query: 183 DLAANQTLMANTVRDAMALSFAQYGLTLDQFNIASFNLPDDLQVALDQRIAAGMKGGLDA 242 DLAANQ L++N VR+A+ F QYGL LD F ++S LPD+LQ ALD+RI+ M G + Sbjct: 181 DLAANQALLSNKVREALLPQFTQYGLALDSFQVSSVTLPDELQAALDRRISMDMTGDMQR 240 Query: 243 AKAAGYARVQAAEAIPIAAGNEGGLAGLGAGLATGVSMGQVMADALGTGAAGAQGHAAAA 302 + + Q AE++P+AA NEGG+AG GAGLA G++MGQ MAD+L T QGHA AA Sbjct: 241 -----FTQYQTAESLPLAARNEGGIAGTGAGLAAGLAMGQAMADSLRT---AVQGHAGAA 292 Query: 303 ----PAAAAGPTPAERLGQLKQLLDQGLISAADYDAAKAEVLKKLIG 345 PAA A P RL +LK+LLD+GLI+ AD+DA KA VLK L G Sbjct: 293 PVVQPAAPAVDDPTARLAKLKELLDKGLITQADFDAGKAAVLKALTG 339