BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.3603.p (452 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774605.1| small-conductance mechanosensitive channel p... 637 e-180 ref|YP_004751885.1| putative mechanosensitive ion channel protei... 417 e-114 ref|YP_001354258.1| mechanosensitive ion channel protein [Janthi... 348 8e-94 ref|YP_001100722.1| putative mechanosensitive ion channel protei... 335 1e-89 ref|YP_002982276.1| mechanosensitive ion channel MscS [Ralstonia... 273 3e-71 >ref|YP_003774605.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] gb|ADJ62697.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] Length = 358 Score = 637 bits (1642), Expect = e-180, Method: Composition-based stats. Identities = 340/358 (94%), Positives = 349/358 (97%) Query: 95 MFPLTIAQVAMRFVFYVLRGIFVKDATVGALLHLSEKVIALLVWLCVVLWITGLWPDFVD 154 MFPLT+AQVAMRFVFYVLRGIFVKDATVGALLHLSEK IA+LVWLCVVLWITGLWPDFVD Sbjct: 1 MFPLTVAQVAMRFVFYVLRGIFVKDATVGALLHLSEKAIAVLVWLCVVLWITGLWPDFVD 60 Query: 155 YLDTTTFPLARHKVSLLTMLQGAASVLVTLIIALWIGTVVENRLMKFSGMHSSLRTVVAR 214 YLD TT PLARHKVSLLTMLQGAASVLVTLIIALWIGTV+ENRLMKFSGMHSSLRTVVAR Sbjct: 61 YLDGTTLPLARHKVSLLTMLQGAASVLVTLIIALWIGTVLENRLMKFSGMHSSLRTVVAR 120 Query: 215 MMRALLILIAFLVSLSLVGIDLTVLSVFGGALGVGIGLGLQKIASSYVSGFVILLERSMV 274 M+RALLILIAFLVSLSLVGIDLTVLSVFGGALGVGIGLGLQKIASSYVSGFVILLERSMV Sbjct: 121 MLRALLILIAFLVSLSLVGIDLTVLSVFGGALGVGIGLGLQKIASSYVSGFVILLERSMV 180 Query: 275 IGDMVAVDKYFGKVTQINTRYTILEGLDGIESVLPNELFVSNPVQNYSLTHRIVRLATQL 334 IGDMVAVDKYFGKVTQINTRYTILEGLDGIESVLPNELFVSNPVQNYSLTHRIVRL+TQL Sbjct: 181 IGDMVAVDKYFGKVTQINTRYTILEGLDGIESVLPNELFVSNPVQNYSLTHRIVRLSTQL 240 Query: 335 TILYQDDVETVLSIMEQAALGVQRVSQQTAPQALLIKIGADGLELELGFWITDPENGRLN 394 TILYQDDVETVLSIMEQAALGVQRVSQQTAPQALLIKIGADGL+LELGFWITDPENGRLN Sbjct: 241 TILYQDDVETVLSIMEQAALGVQRVSQQTAPQALLIKIGADGLDLELGFWITDPENGRLN 300 Query: 395 VLSDVNRAIWAAFKQHGIQVAHPKRDIRIMDERSFRQSTRQEIPDIPEAQNSRTMPDN 452 VLSDVNRAIWAAFKQHGI+VAH KRDIRIMDERSFRQST +E PD+P QNSRTMP N Sbjct: 301 VLSDVNRAIWAAFKQHGIEVAHAKRDIRIMDERSFRQSTGKENPDMPGGQNSRTMPGN 358 >ref|YP_004751885.1| putative mechanosensitive ion channel protein [Collimonas fungivorans Ter331] gb|AEK61062.1| Putative mechanosensitive ion channel protein [Collimonas fungivorans Ter331] Length = 457 Score = 417 bits (1073), Expect = e-114, Method: Composition-based stats. Identities = 242/421 (57%), Positives = 314/421 (74%), Gaps = 13/421 (3%) Query: 35 SRWLRARFVLPGDGEAQSLVMQIGVKSFARVLSPLLALGLLTGAYYILKRGHHAVTLWEI 94 +R LR F + AQ MQIG+ SF++VL+P++ L LL L++ V + + Sbjct: 39 ARLLRRSFSV---STAQPPAMQIGLGSFSKVLTPIITLLLLILVKLALQKWQQPVNVMRL 95 Query: 95 MFPLTIAQVAMRFVFYVLRGIFVKDATVGALLHLSEKVIALLVWLCVVLWITGLWPDFVD 154 M PL + MRFVFYVLR +F +++ VG L L EK +LVW+ ++L+ITG WPD D Sbjct: 96 MIPLVASLALMRFVFYVLRRVFARNSGVGTFLLLFEKSFGVLVWIGLLLYITGWWPDLFD 155 Query: 155 YLDTTTFPLARHKVSLLTMLQGAASVLVTLIIALWIGTVVENRLMKFSGMHSSLRTVVAR 214 YLD+T P+ R+KVSLL ++Q SVLVTL+IALW G +E RLM+ + +HSS+RTVVAR Sbjct: 156 YLDSTVLPIGRYKVSLLAIMQALVSVLVTLVIALWAGAALEERLMRVNTVHSSVRTVVAR 215 Query: 215 MMRALLILIAFLVSLSLVGIDLTVLSVFGGALGVGIGLGLQKIASSYVSGFVILLERSMV 274 M RA LIL+A L+SLSLVGIDLTVLSVFGGALGV +GLGLQKIASSYVSGFVILLERS+ Sbjct: 216 MARAFLILVAVLLSLSLVGIDLTVLSVFGGALGVALGLGLQKIASSYVSGFVILLERSLA 275 Query: 275 IGDMVAVDKYFGKVTQINTRYTILEGLDGIESVLPNELFVSNPVQNYSLTHRIVRLATQL 334 IGDMV V ++GKV QIN+RYT+L+GLDG+++V+PNE+F+ N VQNYSLT+RI+RLAT + Sbjct: 276 IGDMVGVSTFYGKVVQINSRYTVLQGLDGVDTVVPNEVFMINSVQNYSLTNRILRLATHV 335 Query: 335 TILYQDDVETVLSIMEQAALGVQRVSQQTAPQALLIKIGADGLELELGFWITDPENGRLN 394 TI +QDDVE++L ++EQ + V RVS++ PQALL+KIG DGLELE+GFWITDPENGRLN Sbjct: 336 TIEHQDDVESILLLLEQVTVEVDRVSREILPQALLMKIGPDGLELEVGFWITDPENGRLN 395 Query: 395 VLSDVNRAIWAAFKQHGIQVAHPKRDIRIMDERSFRQS-----TRQEIP-----DIPEAQ 444 VLSDVNRAIW + I+VA KRDIR++ E +S ++Q++P D P+ + Sbjct: 396 VLSDVNRAIWRVLQSRQIKVAQVKRDIRMVSEGELHKSNFDAYSQQDLPLDRLRDSPKPK 455 Query: 445 N 445 N Sbjct: 456 N 456 >ref|YP_001354258.1| mechanosensitive ion channel protein [Janthinobacterium sp. Marseille] gb|ABR89482.1| mechanosensitive ion channel protein (mscS family) [Janthinobacterium sp. Marseille] Length = 454 Score = 348 bits (894), Expect = 8e-94, Method: Composition-based stats. Identities = 217/424 (51%), Positives = 297/424 (70%), Gaps = 7/424 (1%) Query: 8 LFTDISTVDLLRQLVVIALSLVGGIGLSRWLRARFVLPGDGEAQSLVMQIGVKSFARVLS 67 L+TD+ L QL+ + L G++R +R +L Q+ + ++GV+SF RVL Sbjct: 13 LWTDLGIPGLRWQLIAMVLCFALAWGIARIIRN--LLATQEPQQNALRRLGVESFTRVLW 70 Query: 68 PLLALGLLTGAYYILKR--GHHAVTLWEIMFPLTIAQVAMRFVFYVLRGIFVKDATVGAL 125 PLL LL A IL+R G V + ++ PL + +R FYV+R +F + A Sbjct: 71 PLLTWLLLAIAKAILERLDGVDGVDVLRVVMPLVASFALIRLAFYVMRRVFARGGKTSAF 130 Query: 126 LHLSEKVIALLVWLCVVLWITGLWPDFVDYLDTTTFPLARHKVSLLTMLQGAASVLVTLI 185 + L E+ A LVW+ V L ITG PD +++LD T PL +HKVS++TMLQ SV VTLI Sbjct: 131 VLLFERSFASLVWIVVALHITGFLPDIIEFLDDTFVPLGKHKVSMMTMLQAVVSVGVTLI 190 Query: 186 IALWIGTVVENRLMKFSGMHSSLRTVVARMMRALLILIAFLVSLSLVGIDLTVLSVFGGA 245 IALW ++E RLM+ GMHSS+R V++RM RA+LILIA LVSLS+VGIDLTVLSVFGGA Sbjct: 191 IALWASAMIEERLMRLDGMHSSMRAVLSRMSRAVLILIALLVSLSMVGIDLTVLSVFGGA 250 Query: 246 LGVGIGLGLQKIASSYVSGFVILLERSMVIGDMVAVDKYFGKVTQINTRYTILEGLDGIE 305 LGVG+GLGLQKIASSYVSGF+ILLERSM IGD V +DK+ G+V QINTRYT+L G DG + Sbjct: 251 LGVGLGLGLQKIASSYVSGFIILLERSMTIGDTVTMDKFTGRVAQINTRYTVLRGGDGSD 310 Query: 306 SVLPNELFVSNPVQNYSLTHRIVRLATQLTILYQDDVETVLSIMEQAALGVQRVSQ--QT 363 ++LPN+LFVSN VQN SL+ +V+L T++++ Y+ D++ L ++E+AAL V++V + Sbjct: 311 TILPNDLFVSNAVQNLSLSDTVVQLTTRISVDYRTDIDLALRLLEEAALSVEKVRKGPDR 370 Query: 364 APQALLIKIGADGLELELGFWITDPENGRLNVLSDVNRAIWAAFKQHGIQVAHPKRDIRI 423 P A ++ GADG++L+LGFW+ D + GR VLSDVNRAIW +F+ + I V P+R+IR+ Sbjct: 371 VPGATMMAFGADGIDLQLGFWVNDLD-GRGTVLSDVNRAIWRSFQANEINVPFPQREIRL 429 Query: 424 MDER 427 +DE+ Sbjct: 430 IDEQ 433 >ref|YP_001100722.1| putative mechanosensitive ion channel protein [Herminiimonas arsenicoxydans] emb|CAL62601.1| Putative mechanosensitive ion channel protein [Herminiimonas arsenicoxydans] Length = 451 Score = 335 bits (858), Expect = 1e-89, Method: Composition-based stats. Identities = 221/441 (50%), Positives = 297/441 (67%), Gaps = 18/441 (4%) Query: 10 TDISTVDLLRQLVVIALSLVGGIGLSRWLRARF---VLPGDGEAQSLVMQIGVKSFARVL 66 TD+ D+ Q++ + L G WL AR VL + + G + FARV Sbjct: 15 TDLGIPDVRWQILALVLCFSVG-----WLLARLGSGVLAAHDAQRKNAPRPGAEKFARVW 69 Query: 67 SPLLALGLLTGAYYILKRGHHAVTLWEIMFPLTIAQVAMRFVFYVLRGIFVKDATVGALL 126 P L L L+ A +L R +V L + PL + V +R FY+LR +F +D + + Sbjct: 70 WPFLTLVLIGIAKPVLAR-WISVDLLRLALPLVASFVLVRLAFYILRRVFARDGKGRSFI 128 Query: 127 HLSEKVIALLVWLCVVLWITGLWPDFVDYLDTTTFPLARHKVSLLTMLQGAASVLVTLII 186 L + A LVW V L ITG+ PD +LD T PL R+KVSL+TMLQ A SV VTL++ Sbjct: 129 LLFGRGFAGLVWTIVALHITGVLPDIAQFLDDTLIPLGRNKVSLMTMLQAALSVGVTLLV 188 Query: 187 ALWIGTVVENRLMKFSGMHSSLRTVVARMMRALLILIAFLVSLSLVGIDLTVLSVFGGAL 246 ALW+ + E RLMK + MHSS+R V++RM RALLILIA L+SLS+VGIDLTVLSVFGGAL Sbjct: 189 ALWLSALAEERLMKLNSMHSSMRAVLSRMGRALLILIALLLSLSMVGIDLTVLSVFGGAL 248 Query: 247 GVGIGLGLQKIASSYVSGFVILLERSMVIGDMVAVDKYFGKVTQINTRYTILEGLDGIES 306 GVG+GLGLQK+ASSYVSGFVILLERS+ IGD V VDK+ G+V+QINTRYT+L G DG ++ Sbjct: 249 GVGLGLGLQKLASSYVSGFVILLERSLAIGDTVIVDKFTGRVSQINTRYTVLRGGDGTDT 308 Query: 307 VLPNELFVSNPVQNYSLTHRIVRLATQLTILYQDDVETVLSIMEQAALGVQRV--SQQTA 364 V+PNE+FVS+PVQN +LT +V++ T++T+ Y+ D++ L ++E+AAL V+RV Sbjct: 309 VVPNEMFVSSPVQNLTLTDTLVQMTTRITVDYRTDIDLALRLLEEAALSVERVRPGPDRG 368 Query: 365 PQALLIKIGADGLELELGFWITDPENGRLNVLSDVNRAIWAAFKQHGIQVAHPKRDIRIM 424 P A+L+ GADG++L+LGFW+ DPE GR VLS+VNRAIW +F+ H I V P+R+IR++ Sbjct: 369 PGAILVAFGADGIDLQLGFWVNDPE-GRGGVLSNVNRAIWRSFQAHDINVPFPQREIRLI 427 Query: 425 DER------SFRQSTRQEIPD 439 DE+ +TR+ PD Sbjct: 428 DEQYRAVKEHLNTNTRRSTPD 448 >ref|YP_002982276.1| mechanosensitive ion channel MscS [Ralstonia pickettii 12D] gb|ACS63604.1| MscS Mechanosensitive ion channel [Ralstonia pickettii 12D] Length = 458 Score = 273 bits (699), Expect = 3e-71, Method: Composition-based stats. Identities = 167/408 (40%), Positives = 261/408 (63%), Gaps = 7/408 (1%) Query: 37 WLRARFV---LPGDGEAQSLVMQIGVKSFARVLSPLLALGLLTGAYYILKRGHHAVTLWE 93 WL AR + L ++ ++ S R + PL+ L+ GA Y L G ++++ Sbjct: 44 WLLARPISRRLHSAHVNETFALRFAWSSLERAMFPLIGWLLVVGARYALV-GTMPISVFR 102 Query: 94 IMFPLTIAQVAMRFVFYVLRGIFVKDATVGALLHLSEKVIALLVWLCVVLWITGLWPDFV 153 + + FYVLR + + + +L L+E+V+ L+W+ +VL++ G+ D V Sbjct: 103 LAAVPLFGLAILYLGFYVLRRVLSANGELHGMLVLAERVLTTLMWIGMVLYVLGVLGDVV 162 Query: 154 DYLDTTTFPLA-RHKVSLLTMLQGAASVLVTLIIALWIGTVVENRLMKFSGMHSSLRTVV 212 DYLD F L + KVSL ML G +LVT+++A+W G+ +++R+ + S + ++L+ V+ Sbjct: 163 DYLDGIRFALGGKQKVSLAAMLMGVVWILVTVLVAMWFGSWLDSRITRASAIDANLKVVL 222 Query: 213 ARMMRALLILIAFLVSLSLVGIDLTVLSVFGGALGVGIGLGLQKIASSYVSGFVILLERS 272 +R+ +A+L+L++ L+SLSLVGIDLTVLSVFGGALGVG+G GLQKIAS+YVSGF+ILLERS Sbjct: 223 SRVTKAVLLLVSLLLSLSLVGIDLTVLSVFGGALGVGLGFGLQKIASNYVSGFIILLERS 282 Query: 273 MVIGDMVAVDKYFGKVTQINTRYTILEGLDGIESVLPNELFVSNPVQNYSLTHRIVRLAT 332 + +GD + V Y G VTQI TRYT++ DG ++++PNEL V+ VQN++ VR+A Sbjct: 283 LKLGDQITVSTYTGIVTQIRTRYTVVRNGDG-DTLVPNELMVAQAVQNHN-ERGTVRVAV 340 Query: 333 QLTILYQDDVETVLSIMEQAALGVQRVSQQTAPQALLIKIGADGLELELGFWITDPENGR 392 ++ Y D E+VL+++ Q A G+ RV + AP A + G+E ELG WI DP NG+ Sbjct: 341 RVQASYDADPESVLALLLQCAEGIPRVLPEPAPAAFMALFADSGIEYELGVWIGDPHNGK 400 Query: 393 LNVLSDVNRAIWAAFKQHGIQVAHPKRDIRIMDERSFRQSTRQEIPDI 440 L V SD+NRAI+ FK+ GI + +P+R++R++ + +Q + + D+ Sbjct: 401 LGVQSDLNRAIYRRFKEAGIDIPYPQREVRVLGASALQQGVTRTLSDV 448