BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.3542.p (309 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003773684.1| VGR-related protein [Herbaspirillum seropedi... 447 e-123 ref|YP_001816411.1| type VI secretion system Vgr family protein ... 285 6e-75 ref|YP_621290.1| Rhs element Vgr protein [Burkholderia cenocepac... 276 2e-72 ref|ZP_02910349.1| rhs element Vgr protein [Burkholderia ambifar... 221 1e-55 ref|YP_004152870.1| type VI secretion system vgr family protein ... 151 1e-34 >ref|YP_003773684.1| VGR-related protein [Herbaspirillum seropedicae SmR1] gb|ADJ61776.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 966 Score = 447 bits (1149), Expect = e-123, Method: Composition-based stats. Identities = 244/309 (78%), Positives = 275/309 (88%) Query: 1 MLEREALLDTLHSLEELAQRLSQDAARYHAEVTDLAQLERIRKQLQAWDTSEAAGGTRHA 60 MLER ALL+TL +L+ELAQRLS DAAR+HAE TD+AQLERIR QL+AWD + A G A Sbjct: 641 MLERAALLETLDNLQELAQRLSHDAARHHAEATDVAQLERIRAQLRAWDAGDVANGAVRA 700 Query: 61 AAPMVALDAPAGVSVTSQDTMVLGASRHIDLVSRDNTQLNAGRKLLMRAGEMFAAFAGKH 120 PMVA+DAPAGVS+TSQDTMVLGA++HIDLVSR NTQL+AGR+LLMRAGEM +AFA KH Sbjct: 701 ETPMVAVDAPAGVSITSQDTMVLGAAQHIDLVSRANTQLSAGRRLLMRAGEMLSAFAAKH 760 Query: 121 MKLISGKGSVKVQAHEEHVELQAARRILLEASEEIILQAPKISIRSRDDMQIHGGGSFSR 180 MKLISGKGS+K+QAHEEH++L AARRILLEASEEIILQAPKISIRSR+ ++ GGGSFSR Sbjct: 761 MKLISGKGSIKIQAHEEHIDLSAARRILLEASEEIILQAPKISIRSRETTEVAGGGSFSR 820 Query: 181 WNASLIEHGTSGQWRQQAAEHNVLGPANCPPPEIAPPPTFEELQQSASLMVRLRSHVRDG 240 WNAS IEHGTSG+WRQQAA H+VLGPANCPPP+I PPPT+EELQQS+SLMVRLRSH RDG Sbjct: 821 WNASAIEHGTSGEWRQQAAMHHVLGPANCPPPDIPPPPTYEELQQSSSLMVRLRSHARDG 880 Query: 241 RPLAHQPYTLFKQGTQIAVGVTDAAGRLRIEGHQPGDDSYSVKLPNGYCFDLPVHEQVSA 300 R LAHQPYTLFKQG QIAVGVTDAAG+LRI+ HQPGDDSYSV+L NGY FDLPV E++ Sbjct: 881 RLLAHQPYTLFKQGEQIAVGVTDAAGQLRIKDHQPGDDSYSVRLSNGYHFDLPVQEELGD 940 Query: 301 LDQQLAASG 309 LD QLAASG Sbjct: 941 LDHQLAASG 949 >ref|YP_001816411.1| type VI secretion system Vgr family protein [Burkholderia ambifaria MC40-6] gb|ACB68858.1| type VI secretion system Vgr family protein [Burkholderia ambifaria MC40-6] Length = 955 Score = 285 bits (729), Expect = 6e-75, Method: Composition-based stats. Identities = 154/310 (49%), Positives = 213/310 (68%), Gaps = 2/310 (0%) Query: 1 MLEREALLDTLHSLEELAQRLSQDAARYHAEVTDLAQLERIRKQLQAWDT-SEAAGGTRH 59 MLER AL + L+EL Q L +A + A+ TDLA++E+I Q+++WD S G Sbjct: 630 MLERTALQTLIEQLQELVQNLGDASAAHDADNTDLARIEKIASQIKSWDAGSNVNQGGGT 689 Query: 60 AAAPMVALDAPAGVSVTSQDTMVLGASRHIDLVSRDNTQLNAGRKLLMRAGEMFAAFAGK 119 APMVA++ PAGV+V SQD MVLGA ID VS NTQ++AGR+LL+R G+ +AFA K Sbjct: 690 GGAPMVAIEGPAGVTVVSQDAMVLGAQTTIDAVSSGNTQISAGRRLLIRVGDWMSAFAAK 749 Query: 120 HMKLISGKGSVKVQAHEEHVELQAARRILLEASEEIILQAPKISIRSRDDMQIHGGGSFS 179 MKL++ G ++++AH+E V ++AA+RI+LEA EEI+ ++PK+S ++ D+ I+GG S+S Sbjct: 750 GMKLVTADGKLRIEAHKEDVIVKAAKRIILEAGEEIVFRSPKVSTQASDEASINGGSSYS 809 Query: 180 RWNASLIEHGTSGQWRQQAAEHNVLGPANCPPPEIAPPPTFEELQQSASLMVRLRSHVRD 239 +W+ S + HGTSG WR+ AA H+++GP N P ++ P +F+EL+Q SL V LRSH Sbjct: 810 QWSGSGVVHGTSGVWREHAASHSLVGPDN-KPVKVPDPVSFKELEQKESLAVVLRSHPDG 868 Query: 240 GRPLAHQPYTLFKQGTQIAVGVTDAAGRLRIEGHQPGDDSYSVKLPNGYCFDLPVHEQVS 299 GRPLA++PYTL+K +IA GVTD G+L I HQ G SY VKL NG+ D+PV E Sbjct: 869 GRPLAYEPYTLYKGAAKIADGVTDEHGQLIIANHQKGTSSYMVKLHNGHEIDVPVMEGAL 928 Query: 300 ALDQQLAASG 309 D QLAA G Sbjct: 929 TDDDQLAAEG 938 >ref|YP_621290.1| Rhs element Vgr protein [Burkholderia cenocepacia AU 1054] ref|YP_840041.1| Rhs element Vgr protein [Burkholderia cenocepacia HI2424] gb|ABF76317.1| Rhs element Vgr protein [Burkholderia cenocepacia AU 1054] gb|ABK13148.1| Rhs element Vgr protein [Burkholderia cenocepacia HI2424] Length = 956 Score = 276 bits (707), Expect = 2e-72, Method: Composition-based stats. Identities = 151/311 (48%), Positives = 210/311 (67%), Gaps = 3/311 (0%) Query: 1 MLEREALLDTLHSLEELAQRLSQDAARYHAEVTDLAQLERIRKQLQAWDTSEAA--GGTR 58 MLER AL + L+EL + L +A A+ TDLA++E+I Q++ WD GG Sbjct: 630 MLERTALQTLIEQLQELVRNLGDASAANDADNTDLARIEKITSQIKGWDAGSNVDQGGGG 689 Query: 59 HAAAPMVALDAPAGVSVTSQDTMVLGASRHIDLVSRDNTQLNAGRKLLMRAGEMFAAFAG 118 APMVA++ PAGV+V SQD MVLGA +ID VS NTQ++ GR+LLMR G+ +AFA Sbjct: 690 SGGAPMVAIEGPAGVTVVSQDAMVLGAQTNIDAVSAGNTQISVGRRLLMRVGDWMSAFAA 749 Query: 119 KHMKLISGKGSVKVQAHEEHVELQAARRILLEASEEIILQAPKISIRSRDDMQIHGGGSF 178 K M L++ G ++++AH+E V ++AA+RI+LEA EEI+ ++PK++ ++ D+ I+GG S+ Sbjct: 750 KGMTLVTADGKLRIEAHKEDVVVKAAKRIILEAGEEIVFRSPKVNTQASDEASINGGSSY 809 Query: 179 SRWNASLIEHGTSGQWRQQAAEHNVLGPANCPPPEIAPPPTFEELQQSASLMVRLRSHVR 238 S+WN + HGTSG WR+ AA H+++GP N P + P TF+EL+Q S V LRSH Sbjct: 810 SQWNGGGVVHGTSGVWREHAASHSLVGPDN-KPVKAPDPLTFKELEQQDSFAVVLRSHPS 868 Query: 239 DGRPLAHQPYTLFKQGTQIAVGVTDAAGRLRIEGHQPGDDSYSVKLPNGYCFDLPVHEQV 298 DGRPLA++PYTL+K +IA GVTD G+L I HQ G SY+VKL NG+ ++PV E Sbjct: 869 DGRPLAYEPYTLYKGAAKIADGVTDEHGQLIIANHQKGTSSYTVKLHNGHEIEVPVMEGG 928 Query: 299 SALDQQLAASG 309 + D QLAA G Sbjct: 929 LSDDDQLAAQG 939 >ref|ZP_02910349.1| rhs element Vgr protein [Burkholderia ambifaria MEX-5] gb|EDT38528.1| rhs element Vgr protein [Burkholderia ambifaria MEX-5] Length = 245 Score = 221 bits (563), Expect = 1e-55, Method: Composition-based stats. Identities = 118/229 (51%), Positives = 160/229 (69%), Gaps = 1/229 (0%) Query: 81 MVLGASRHIDLVSRDNTQLNAGRKLLMRAGEMFAAFAGKHMKLISGKGSVKVQAHEEHVE 140 MVLGA ID VS NTQ++AGR+LL+R G+ +AFA K MKLI+ G ++++AH+E V Sbjct: 1 MVLGAQTTIDAVSSGNTQISAGRRLLIRVGDWMSAFAAKGMKLITADGKLRIEAHKEDVI 60 Query: 141 LQAARRILLEASEEIILQAPKISIRSRDDMQIHGGGSFSRWNASLIEHGTSGQWRQQAAE 200 ++AA+RI+LEA EEI+ ++PK+S ++ D+ I+GG S+S+WN S + HGTSG WR+ A Sbjct: 61 VKAAKRIILEAGEEIVFRSPKVSTQASDEASINGGSSYSQWNGSGVVHGTSGVWREHATS 120 Query: 201 HNVLGPANCPPPEIAPPPTFEELQQSASLMVRLRSHVRDGRPLAHQPYTLFKQGTQIAVG 260 H+++GP N P + P +F+EL+Q SL V LRSH GRPLA++PYTL+K +IA G Sbjct: 121 HSLVGPDN-KPVKAPDPVSFKELEQKESLAVVLRSHPDGGRPLAYEPYTLYKGAAKIADG 179 Query: 261 VTDAAGRLRIEGHQPGDDSYSVKLPNGYCFDLPVHEQVSALDQQLAASG 309 VTD G+L I HQ G SY VKL NG+ D+PV E D QLAA G Sbjct: 180 VTDEHGQLIIANHQKGTSSYMVKLHNGHEIDVPVMEGALTDDDQLAAEG 228 >ref|YP_004152870.1| type VI secretion system vgr family protein [Variovorax paradoxus EPS] gb|ADU34759.1| type VI secretion system Vgr family protein [Variovorax paradoxus EPS] Length = 944 Score = 151 bits (382), Expect = 1e-34, Method: Composition-based stats. Identities = 94/275 (34%), Positives = 161/275 (58%), Gaps = 12/275 (4%) Query: 38 LERIRKQLQAWDTSEAAGGTR--HAAAPMVALDAPAGVSVTSQDTMVLGASRHIDLVSRD 95 ++ I+ Q A ++ AAG R A P + L +PAG+ T+ + + ++ H L S Sbjct: 654 IQSIKAQNDAIKSTAAAGKDRFPELADPHLVLASPAGIETTTSQSTHIASAEHNALTSGG 713 Query: 96 NTQLNAGRKLLMRAGEMFAAFAGKH-MKLISGKGSVKVQAHEEHVELQAARRILLEASEE 154 +T ++A + L+ A + FA + ++L + G++ ++A +E + + A + Sbjct: 714 HTSVSAAKSFLVSAKQAVRLFAYQAGLRLFAFGGNIDIKALKESINILAKL--------D 765 Query: 155 IILQAPKISIRSRDDMQIHGGGSFSRWNASLIEHGTSGQWRQQAAEHNVLGPANCPPPEI 214 I+ A +I+I +++++ ++GGGS R NA+ IE TSG+ + AA H+++GP N PP + Sbjct: 766 IVQVANRITITAKEELVLNGGGSTIRLNAAGIEDATSGKHVEHAAVHSMVGPKNAPPRSV 825 Query: 215 APPPTFEELQQSASLMVRLRSHVRDGRPLAHQPYTLFKQGTQIAVGVTDAAGRLRIEGHQ 274 + + + L++ +L L SH +DGRPL +PYTL++ G +A GVTDA G++ IE HQ Sbjct: 826 SGMSSLD-LKKKGALSFVLLSHAQDGRPLGFEPYTLYRDGANVADGVTDADGQVLIEDHQ 884 Query: 275 PGDDSYSVKLPNGYCFDLPVHEQVSALDQQLAASG 309 SY V+L NG ++LPV Q+ + D +LAASG Sbjct: 885 SDTTSYKVRLANGNEYELPVKAQLKSNDDRLAASG 919