BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.3434.p (341 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_349627.1| D-isomer specific 2-hydroxyacid dehydrogenase [... 263 3e-68 ref|YP_002873588.1| putative 2-hydroxyacid dehydrogenase [Pseudo... 261 8e-68 ref|YP_001862641.1| D-isomer specific 2-hydroxyacid dehydrogenas... 261 1e-67 gb|EGH86076.1| D-isomer specific 2-hydroxyacid dehydrogenase fam... 259 3e-67 ref|YP_004354832.1| dehydrogenase [Pseudomonas brassicacearum su... 258 7e-67 >ref|YP_349627.1| D-isomer specific 2-hydroxyacid dehydrogenase [Pseudomonas fluorescens Pf0-1] gb|ABA75636.1| putative 2-hydroxyacid dehydrogenase [Pseudomonas fluorescens Pf0-1] Length = 309 Score = 263 bits (672), Expect = 3e-68, Method: Composition-based stats. Identities = 141/295 (47%), Positives = 192/295 (65%), Gaps = 2/295 (0%) Query: 47 FREEAARRMPELQVRLWPDLPDPDDVEAVAGWYPPAGVIERLPNLRLISSIAAGVDHVLG 106 +E ARR P+L L D P + + A W+P + + LPNL++I S+AAG+DH L Sbjct: 17 LQEAFARRAPQLSAVLADD-PRAVNAQIAACWFPLSDSLAALPNLQVIHSVAAGIDH-LE 74 Query: 107 AARAHPLVPVCRIVDPALAAGMAEYVLWAVLYYHRALDQALQQQVQRVWKIPSQKPAYQT 166 + P +PVCR+VDP GM EYV WAV+++HR DQ L+QQ Q+ W+ P Q+PA++ Sbjct: 75 HDPSCPDLPVCRVVDPGHRQGMTEYVRWAVIHFHRGFDQVLEQQRQQHWERPLQRPAHEF 134 Query: 167 HVGLMGLGALGSHLAGVLRDQGFTVSGWSRSAHHIEGVRTYAGDDALHAFLQAPDIVVCL 226 +G+MGLG+LGS + L G+ V GW+RS+ ++ GV+TYAG DA + FL+ ++++ L Sbjct: 135 RIGVMGLGSLGSAIVQDLASAGYDVRGWARSSKNLPGVQTYAGIDAFNPFLEGVELLINL 194 Query: 227 LPLTDATRKLLGRDVFAAMKPGAALVHCGRGEHLVEEDLLEALDRQHLRGAVLDVFAQEP 286 LPLT TR +L R F + GAALV+ GRG HL +DL +AL R LRGA+LDVF QEP Sbjct: 195 LPLTHETRGILNRQTFERLGNGAALVNVGRGGHLNIDDLQQALARGKLRGALLDVFEQEP 254 Query: 287 LPASNALWSHPRVVVTPHMASSAQPGVIVGQILDNLQRLRRGEAALHEVDRSRGY 341 LPA + LW P V +TPHMAS+A I QI +N +RL GE L+ DR GY Sbjct: 255 LPADHPLWKTPGVTITPHMASAASHDCIAEQIAENFRRLNAGEPLLNSADRLLGY 309 >ref|YP_002873588.1| putative 2-hydroxyacid dehydrogenase [Pseudomonas fluorescens SBW25] emb|CAY50457.1| putative 2-hydroxyacid dehydrogenase [Pseudomonas fluorescens SBW25] Length = 308 Score = 261 bits (668), Expect = 8e-68, Method: Composition-based stats. Identities = 141/298 (47%), Positives = 193/298 (64%), Gaps = 5/298 (1%) Query: 44 HAVFREEAARRMPELQVRLWPDLPDPDDVEAVAGWYPPAGVIERLPNLRLISSIAAGVDH 103 A+F E A P+++ R WPD+ +P+DVE +A W P + + LPNL+++ +++AGVD Sbjct: 16 QALFAEHA----PDIEWRAWPDIGNPEDVEYLAAWQAPEDLAQVLPNLKVLFALSAGVDQ 71 Query: 104 VLGAARAHPLVPVCRIVDPALAAGMAEYVLWAVLYYHRALDQALQQQVQRVWKIPSQKPA 163 L +R P +PV R++DP + GM EY WAVL HR + + QQQV R W+ +PA Sbjct: 72 -LDLSRLPPQLPVVRLLDPGITRGMCEYASWAVLSLHRDMLRYRQQQVARCWQAHLLQPA 130 Query: 164 YQTHVGLMGLGALGSHLAGVLRDQGFTVSGWSRSAHHIEGVRTYAGDDALHAFLQAPDIV 223 VG+MGLGA + L GF +SGW+RSAH + GV YAG+ L AFL DIV Sbjct: 131 AHRRVGVMGLGAQAQQILATLAPLGFALSGWARSAHRVPGVACYAGEAQLPAFLSQCDIV 190 Query: 224 VCLLPLTDATRKLLGRDVFAAMKPGAALVHCGRGEHLVEEDLLEALDRQHLRGAVLDVFA 283 +C+LPLT+ T+ +L R +FA + GAAL++ GRG HLVEEDLLEAL L GAVLDV Sbjct: 191 LCVLPLTEQTQGILDRRLFAQLPWGAALINMGRGGHLVEEDLLEALGSGQLSGAVLDVLR 250 Query: 284 QEPLPASNALWSHPRVVVTPHMASSAQPGVIVGQILDNLQRLRRGEAALHEVDRSRGY 341 +EP A + W HP+V++TPH+A+ QP G +L+N++R RGE + EVDR RGY Sbjct: 251 EEPAAADHPFWGHPQVLLTPHIAAMTQPESAFGVLLENIRRFERGEEMVGEVDRGRGY 308 >ref|YP_001862641.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding [Burkholderia phymatum STM815] gb|ACC75595.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding [Burkholderia phymatum STM815] Length = 308 Score = 261 bits (666), Expect = 1e-67, Method: Composition-based stats. Identities = 146/291 (50%), Positives = 188/291 (64%), Gaps = 3/291 (1%) Query: 52 ARRMPELQVRLWPDLPDPDDVEAVAGWYPPAGVIERLPNLRLISSIAAGVDHVLGAARAH 111 A + P++ RLWPD+ DP V +A W PP LPNL +I S+ AG+D +R Sbjct: 20 AHKAPDIAFRLWPDIGDPASVRYLAAWQPPDDPARTLPNLEVIFSVGAGIDQ-FDLSRVP 78 Query: 112 PLVPVCRIVDPALAAGMAEYVLWAVLYYHRAL-DQALQQQVQRVWKIPSQKPAYQTHVGL 170 P + V R+++P + GM EYV AVL HR L D ALQQ+ QRVW+ + A VG+ Sbjct: 79 PHIAVVRMMEPGIVEGMVEYVTQAVLTIHRDLFDYALQQR-QRVWRELPVRAAATRRVGV 137 Query: 171 MGLGALGSHLAGVLRDQGFTVSGWSRSAHHIEGVRTYAGDDALHAFLQAPDIVVCLLPLT 230 +GLG LG+ + LR GF +GWSRSAH IEGV +AG DAL AFL DI+VCLLPLT Sbjct: 138 LGLGMLGTAVLERLRLFGFPCAGWSRSAHSIEGVECHAGMDALDAFLGRTDILVCLLPLT 197 Query: 231 DATRKLLGRDVFAAMKPGAALVHCGRGEHLVEEDLLEALDRQHLRGAVLDVFAQEPLPAS 290 DATR LL R FA + GA+ +H GRG ++DLL+ALD L+ A+LDV EPLP S Sbjct: 198 DATRGLLDRHRFAKLPKGASFIHVGRGPQANQQDLLDALDSGQLQNAILDVTDPEPLPES 257 Query: 291 NALWSHPRVVVTPHMASSAQPGVIVGQILDNLQRLRRGEAALHEVDRSRGY 341 + LW+HPRV +TPH+AS+ +P V +LDN++R R G L EVDR+RGY Sbjct: 258 HPLWTHPRVRITPHVASATRPETAVDVVLDNIRRHRDGLPMLGEVDRTRGY 308 >gb|EGH86076.1| D-isomer specific 2-hydroxyacid dehydrogenase family protein [Pseudomonas syringae pv. lachrymans str. M301315] Length = 380 Score = 259 bits (663), Expect = 3e-67, Method: Composition-based stats. Identities = 138/319 (43%), Positives = 195/319 (61%), Gaps = 5/319 (1%) Query: 23 TEGHAMPTLLICSATDDLSDLHAVFREEAARRMPELQVRLWPDLPDPDDVEAVAGWYPPA 82 T+ MP LL + +F E A P+++ R+WPD+ DP D+ + W P Sbjct: 67 TQEKPMPALLYKADPARAERWRVLFAEHA----PDIEWRVWPDIGDPADIHYLGAWLAPD 122 Query: 83 GVIERLPNLRLISSIAAGVDHVLGAARAHPLVPVCRIVDPALAAGMAEYVLWAVLYYHRA 142 + E LPNL+++ +++AGVD L +R +PV R++DP ++ GM +Y AVL HR Sbjct: 123 DLQELLPNLKVLFALSAGVDQ-LDLSRIPESLPVVRLLDPGISHGMCQYASLAVLSLHRE 181 Query: 143 LDQALQQQVQRVWKIPSQKPAYQTHVGLMGLGALGSHLAGVLRDQGFTVSGWSRSAHHIE 202 + + QQQV+ VWK PA++ VG+MGLG H+ L+ GF +SGW+RS H +E Sbjct: 182 MLRYRQQQVEGVWKAHPLVPAHKRRVGVMGLGLQAQHILSTLKPFGFELSGWARSEHQVE 241 Query: 203 GVRTYAGDDALHAFLQAPDIVVCLLPLTDATRKLLGRDVFAAMKPGAALVHCGRGEHLVE 262 GV YAG+ L AFL DI++C+LPLT T +L R +F + GAAL++ GRG HLVE Sbjct: 242 GVTCYAGEGQLSAFLGQCDILLCVLPLTGQTEGILNRQLFERLPQGAALINMGRGGHLVE 301 Query: 263 EDLLEALDRQHLRGAVLDVFAQEPLPASNALWSHPRVVVTPHMASSAQPGVIVGQILDNL 322 DLL+ALD L AVLDV QEP PA + W HP++++TPH+A+ QP +LDN+ Sbjct: 302 ADLLDALDSGQLSAAVLDVLQQEPAPADHPFWQHPKIMLTPHVAAMTQPESAFPGLLDNI 361 Query: 323 QRLRRGEAALHEVDRSRGY 341 +R RGEA +VDR +GY Sbjct: 362 RRFERGEAMQGQVDRGQGY 380 >ref|YP_004354832.1| dehydrogenase [Pseudomonas brassicacearum subsp. brassicacearum NFM421] gb|AEA69828.1| Putative dehydrogenase [Pseudomonas brassicacearum subsp. brassicacearum NFM421] Length = 308 Score = 258 bits (660), Expect = 7e-67, Method: Composition-based stats. Identities = 136/295 (46%), Positives = 189/295 (64%), Gaps = 1/295 (0%) Query: 47 FREEAARRMPELQVRLWPDLPDPDDVEAVAGWYPPAGVIERLPNLRLISSIAAGVDHVLG 106 +R A P+++ R WPD+ DP D++ +A W P + LPNLR++ +++AGVD L Sbjct: 15 WRRLFAEHAPDIEWRAWPDIGDPRDIQYLAAWQAPDDIETLLPNLRVLFALSAGVDQ-LD 73 Query: 107 AARAHPLVPVCRIVDPALAAGMAEYVLWAVLYYHRALDQALQQQVQRVWKIPSQKPAYQT 166 +R +PV R++DP ++ GM EY +AVL HR + + QQQ +R W+ PA Q Sbjct: 74 LSRLPTTLPVVRLLDPGISQGMCEYASFAVLSLHRDMLRYRQQQAERCWQAHRLVPAGQR 133 Query: 167 HVGLMGLGALGSHLAGVLRDQGFTVSGWSRSAHHIEGVRTYAGDDALHAFLQAPDIVVCL 226 VG+MGLG + L+ GF +SGW+RS HHI+GV +AGD+ L AFL +IV+C+ Sbjct: 134 RVGVMGLGLQARQILATLKTFGFALSGWARSEHHIDGVACFAGDEQLPAFLGQCEIVLCV 193 Query: 227 LPLTDATRKLLGRDVFAAMKPGAALVHCGRGEHLVEEDLLEALDRQHLRGAVLDVFAQEP 286 LPLT+ TR +L R +F + GAALV+ GRG HLVEEDLLEAL L AVLDV QEP Sbjct: 194 LPLTEQTRGILNRQLFQHLPKGAALVNMGRGGHLVEEDLLEALADGQLSAAVLDVLEQEP 253 Query: 287 LPASNALWSHPRVVVTPHMASSAQPGVIVGQILDNLQRLRRGEAALHEVDRSRGY 341 PA + W HP +++TPH+A+ QP G +L+N++R +RGE+ L VDR R Y Sbjct: 254 APADHPFWHHPHILLTPHIAAMTQPESAFGVLLENIRRHQRGESMLGLVDRERSY 308