BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.3417.p (312 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|XP_002536436.1| carboxymethylenebutenolidase, putative [Rici... 469 e-130 ref|YP_003447442.1| hypothetical protein AZL_002600 [Azospirillu... 166 4e-39 ref|YP_724629.1| dienelactone hydrolase or related enzyme [Ralst... 150 3e-34 ref|YP_582202.1| dienelactone hydrolase-like protein [Cupriavidu... 148 1e-33 ref|YP_004683950.1| dienelactone hydrolase [Cupriavidus necator ... 146 5e-33 >ref|XP_002536436.1| carboxymethylenebutenolidase, putative [Ricinus communis] gb|EEF25946.1| carboxymethylenebutenolidase, putative [Ricinus communis] Length = 318 Score = 469 bits (1207), Expect = e-130, Method: Composition-based stats. Identities = 243/314 (77%), Positives = 273/314 (86%), Gaps = 3/314 (0%) Query: 1 MRHWGKPLAAFCIAA-SLAVIGSWAQAAPNSAPPPPLEARRVSLTSVDSKGGKATALYGV 59 MR G L +FC+ A A AAPNSAPP PL A RVSLTS+D KGGKATALYGV Sbjct: 1 MRIRGTSLVSFCVGALFFAAAPKLVHAAPNSAPPAPLVAERVSLTSIDDKGGKATALYGV 60 Query: 60 WIKARKSLTT--KGPAPTVIALHGCGGLYSMVKDGKGEFTPRHLAMARVLSDAGYNVLFP 117 WIKARKSL+T KG PTVIALHGCGGLYS V++GKGEFTPRHLAMAR L+DAGYNVLFP Sbjct: 61 WIKARKSLSTDKKGKYPTVIALHGCGGLYSTVRNGKGEFTPRHLAMARALTDAGYNVLFP 120 Query: 118 DSFTPRGRRSTCQDTVAQREVSAMNRRYDVQAALHWVTSQPDVDVKRIALLGWSHGASTL 177 DSFTPRGRRS CQD+VAQRE S MNRR+DVQAAL W +QPD+D+ R+ALLGWS GAST+ Sbjct: 121 DSFTPRGRRSNCQDSVAQREASVMNRRHDVQAALRWAATQPDIDMSRLALLGWSQGASTV 180 Query: 178 LASINLAETDVAVRKIQPRAAVAFYPDCRPYAKTTEPFKPAAPLLILMGENDDWTPPQAC 237 LA++NLA+TDVAVRK+QPRAA+AFYP+C+PYAK PFKPAAPLL+LMGENDDWTPPQAC Sbjct: 181 LAAMNLADTDVAVRKVQPRAAIAFYPNCQPYAKPGTPFKPAAPLLVLMGENDDWTPPQAC 240 Query: 238 EAIEEKMKGSDTEIALRLYPDTYHDFDAPGLPVHVRMDVAGVGKRGEGVTSGGNPDARSQ 297 EA+E+KM+GSDTEIALRLYPDTYHDFDAPGLPVHVRMD++G+GK G GVTSG NPDAR+ Sbjct: 241 EAMEKKMEGSDTEIALRLYPDTYHDFDAPGLPVHVRMDISGIGKPGAGVTSGANPDARAM 300 Query: 298 AYRSMLNFLDAKLN 311 AYRSMLNFL+AKLN Sbjct: 301 AYRSMLNFLEAKLN 314 >ref|YP_003447442.1| hypothetical protein AZL_002600 [Azospirillum sp. B510] dbj|BAI70898.1| hypothetical protein AZL_002600 [Azospirillum sp. B510] Length = 346 Score = 166 bits (421), Expect = 4e-39, Method: Composition-based stats. Identities = 108/276 (39%), Positives = 147/276 (53%), Gaps = 14/276 (5%) Query: 37 EARRVSLTSVDSKGGKATALYGVWIKARKSLTTKGPAPTVIALHGCGGLYSMVKDGKGEF 96 E R + D GG T L G ++ GP P V+ LHGCGGLY+ G Sbjct: 81 EIVRFPSSDGDLTGGAPTMLSGRLLRP----AGNGPHPAVVMLHGCGGLYAR----NGRV 132 Query: 97 TPRHLAMARVLSDAGYNVLFPDSFTPRGRRSTCQDTVAQREVSA-MNRRYDVQAALHWVT 155 +PRH+ A L GY VL DSF PR C + R V A + R+ D AAL ++ Sbjct: 133 SPRHVWWATHLQRQGYEVLMVDSFGPRDVEEVCTTPLKDRTVRATVERKRDAWAALAFLR 192 Query: 156 SQPDVDVKRIALLGWSHGASTLLASINLAETDVAVRKIQP-RAAVAFYPDCRPYAKTTEP 214 +P+VD +R+ L+GWS G T++A+ E AV + RAAVAFYP C+ + E Sbjct: 193 KRPEVDHERVGLIGWSQGGGTVMAAYGAGEDGPAVTEGGGFRAAVAFYPGCKT-PLSNED 251 Query: 215 FKPAAPLLILMGENDDWTPPQACEAIEEKMKGSDTEIALRLYPDTYHDFDAPGLPVHVRM 274 ++P PLL+L+G DDWTPP++C + + G ++ L +YPD YH FD+P PV R Sbjct: 252 WQPDGPLLLLVGGKDDWTPPESCIELAGR-DGIKDKVELVVYPDAYHGFDSPDAPVRKRK 310 Query: 275 DVAGVGKRGEGVTSGGNPDARSQAYRSMLNFLDAKL 310 D+A V G G N DAR+ A R + +F KL Sbjct: 311 DLATV--PGGTAHVGTNEDARTDAIRRVGDFFKEKL 344 >ref|YP_724629.1| dienelactone hydrolase or related enzyme [Ralstonia eutropha H16] emb|CAJ91261.1| dienelactone hydrolase or related enzyme [Ralstonia eutropha H16] Length = 343 Score = 150 bits (378), Expect = 3e-34, Method: Composition-based stats. Identities = 112/301 (37%), Positives = 151/301 (50%), Gaps = 27/301 (8%) Query: 24 AQAAPNSAPPPPLEARRVSLTSVDSKGGKAT-ALYGVWIKARKSLTTKGPAPTVIALHGC 82 ++AAP SA P+ A+RV L +G AT AL W R+ T + P V+ALHGC Sbjct: 47 SRAAPGSAATAPVPAQRVQL-----RGDAATPALTAYWFLPREGATPRA-LPVVVALHGC 100 Query: 83 GGLYSMVK--------------DGKGEFTPRHLAMARVLSDAGYNVLFPDSFTPRGR-RS 127 GGL S D G R+ A L++ GY VL PDSF+ RGR Sbjct: 101 GGLLSARPARASAAGAAGTAQADVSGLLQQRYREYAHWLNERGYAVLMPDSFSARGRPHG 160 Query: 128 TCQDTVAQREVSAMNRRYDVQAALHWVTSQPDVDVKRIALLGWSHGASTLLASINLAETD 187 C + + R + RR D AAL WV QP VD RI LLGWS+GA +LA+++ A Sbjct: 161 ICSEPIDARGIDDRTRRADALAALRWVAQQPAVDTARIVLLGWSNGAQAVLATVD-ASRP 219 Query: 188 VAVRKIQPRAAVAFYPDCRPYAKTTEPFKPAAPLLILMGENDDWTPPQACEAIEEKM--K 245 Q AVAFYP CR A F+ AP+L+++G DDWTP C ++ + + Sbjct: 220 WPAGTPQVERAVAFYPGCR-RAVQQHNFRLRAPMLLMIGGADDWTPATRCAMLQSAVLAR 278 Query: 246 GSDTEIALRLYPDTYHDFDAPGLPVHVRMDVAGVGKRGEGVTSGGNPDARSQAYRSMLNF 305 L +YP YH FD +H+R DV ++ +GVT GG+ AR A + ++ Sbjct: 279 QPGARFRLEIYPGAYHGFDGTS-ELHMRRDVPAGMRKTQGVTVGGDAVARVAALAQLDSW 337 Query: 306 L 306 L Sbjct: 338 L 338 >ref|YP_582202.1| dienelactone hydrolase-like protein [Cupriavidus metallidurans CH34] gb|ABF06933.1| dienelactone hydrolase or related enzyme [Cupriavidus metallidurans CH34] Length = 356 Score = 148 bits (373), Expect = 1e-33, Method: Composition-based stats. Identities = 103/272 (37%), Positives = 137/272 (50%), Gaps = 20/272 (7%) Query: 51 GKATALYGVWIKARKS-LTTKGPAPTVIALHGCGGLYSM------------VKDGKGEFT 97 G + L G W R L P P V+ALHGC GL M V D G Sbjct: 84 GGSPELTGYWFTPRDGGLPRSQPLPVVVALHGCNGLIDMRVAAKRAGTIEEVIDPAGALQ 143 Query: 98 PRHLAMARVLSDAGYNVLFPDSFTPRGR-RSTCQDTVAQREVSAMNRRYDVQAALHWVTS 156 R+ A L++ GY VL PDS++ RGR + TC D + R + RR D AA+ WV+ Sbjct: 144 SRYREYAHWLAERGYAVLMPDSYSVRGRAQGTCGDALESRAIDDRTRRSDALAAMRWVSR 203 Query: 157 QPDVDVKRIALLGWSHGASTLLASINLAETDVAVRKIQPRAAVAFYPDCRPYAKTTEPFK 216 QP +D +RI LLGWS+GA +LA+I+ + R AVAFYP C+ A F+ Sbjct: 204 QPGIDARRIVLLGWSNGAQAVLATIDASRPWPGGTPTVDR-AVAFYPGCK-RAVQQHNFR 261 Query: 217 PAAPLLILMGENDDWTPPQACEAIEEKMKGSDTEIALRL--YPDTYHDFDAPGLPVHVRM 274 +PLL+L+G DDWTP C ++ ++ RL YP YH FD +HVR Sbjct: 262 LRSPLLLLIGGADDWTPATRCAMLQNAVQSRQPGARFRLEIYPGAYHGFDGTS-ELHVRR 320 Query: 275 DVAGVGKRGEGVTSGGNPDARSQAYRSMLNFL 306 DV +R GVT GG+ AR A + ++L Sbjct: 321 DVIA-ARRNGGVTVGGDAVARVAALGQLDSWL 351 >ref|YP_004683950.1| dienelactone hydrolase [Cupriavidus necator N-1] gb|AEI75469.1| dienelactone hydrolase [Cupriavidus necator N-1] Length = 363 Score = 146 bits (368), Expect = 5e-33, Method: Composition-based stats. Identities = 110/301 (36%), Positives = 151/301 (50%), Gaps = 27/301 (8%) Query: 24 AQAAPNSAPPPPLEARRVSLTSVDSKGGKAT-ALYGVWIKARKSLTTKGPAPTVIALHGC 82 ++AAP SA + A+RV L +G AT AL W R+ T++ P V+ALHGC Sbjct: 67 SRAAPGSAATASVPAQRVEL-----RGDAATPALTAYWFLPREG-TSQRALPVVVALHGC 120 Query: 83 GGLYSM--------------VKDGKGEFTPRHLAMARVLSDAGYNVLFPDSFTPRGR-RS 127 GGL S D G R+ A L++ GY VL PDSF+ RGR Sbjct: 121 GGLLSARPSRASAAGAAGTDKADVSGMLQQRYREYAHWLTERGYAVLMPDSFSARGRPHG 180 Query: 128 TCQDTVAQREVSAMNRRYDVQAALHWVTSQPDVDVKRIALLGWSHGASTLLASINLAETD 187 C D + R + RR D AAL WV QP VD RI LLGWS+GA +LA+++ A Sbjct: 181 ICSDPIDTRGIDDRTRRADALAALRWVAQQPAVDTARIVLLGWSNGAQAVLATVD-ASRP 239 Query: 188 VAVRKIQPRAAVAFYPDCRPYAKTTEPFKPAAPLLILMGENDDWTPPQACEAIEEKM--K 245 Q AVAFYP C+ A ++ AP+L+++G DDWTP C ++ + + Sbjct: 240 WPAGTPQVERAVAFYPGCK-RAVQQHNYRLRAPMLLMIGGADDWTPATRCAMLQSAVLAR 298 Query: 246 GSDTEIALRLYPDTYHDFDAPGLPVHVRMDVAGVGKRGEGVTSGGNPDARSQAYRSMLNF 305 L +YP YH FD +H+R DV ++ +GVT GG+ AR A + ++ Sbjct: 299 QPGARFRLEIYPGAYHGFDGTS-ELHMRGDVPAGMRKTQGVTVGGDAVARVAALAQLDSW 357 Query: 306 L 306 L Sbjct: 358 L 358