BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.3363.p (447 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003776280.1| hemolysin secretion-related transmembrane pr... 567 e-159 ref|YP_902032.1| HlyD family type I secretion membrane fusion pr... 461 e-127 emb|CBJ39920.1| Type I secretion adaptor protein (HlyD family) [... 444 e-122 ref|NP_521852.1| hemolysin secretion-like transmembrane protein ... 440 e-121 ref|YP_003747521.1| hemolysin D [Ralstonia solanacearum CFBP2957... 440 e-121 >ref|YP_003776280.1| hemolysin secretion-related transmembrane protein [Herbaspirillum seropedicae SmR1] gb|ADJ64372.1| hemolysin secretion-related transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 567 bits (1462), Expect = e-159, Method: Composition-based stats. Identities = 325/447 (72%), Positives = 386/447 (86%) Query: 1 MSLHHRSRAWLALWQRYGRVLRHAWRQRDPRKSGFYNEQEAEFLPAALSLQESPDSASLR 60 MSL HR +AW LW+RY RV HAWRQR +S F+N+ EAEFLPAALSLQE+PDS SLR Sbjct: 1 MSLRHRWQAWTDLWRRYLRVFGHAWRQRGHYRSDFFNKDEAEFLPAALSLQEAPDSRSLR 60 Query: 61 WTARLLMAMVLLTLLWSLVGHIDIVVNATGKVIPSARIKTIASIEVASVRDILVREGQRV 120 WTARLL+ MV+ LLWSL+G IDIVV+A GKVIPSAR KTIAS++V++VR ILV EGQ V Sbjct: 61 WTARLLVGMVIFALLWSLLGRIDIVVSANGKVIPSARTKTIASVDVSAVRAILVSEGQLV 120 Query: 121 RVGQILMELDATSSDAERDKAADAVAQARLQVARAAAMMQAVQELQAPQLAEVGGAGRAQ 180 + G++L+ELD++SSDAERDKAADAVAQARLQ ARA AMM+A+Q L+ P+LA+V G +AQ Sbjct: 121 KAGEVLIELDSSSSDAERDKAADAVAQARLQAARAQAMMEAIQNLRTPELAQVPGVAQAQ 180 Query: 181 RESAERQLQGQYQDFRARLARIDDEITRYAAALQLASARATDYKELMADNTVSRHAWLEK 240 E+ QL QYQDFRARL R+DDEI+RY AAL+LA+ RA+DY+ L+AD+TVSRHAWLEK Sbjct: 181 WEAVRSQLVAQYQDFRARLGRLDDEISRYGAALKLATQRASDYQTLVADHTVSRHAWLEK 240 Query: 241 EQARVDLQGQLSEARNQRTALLAQTQKEAHDSRIEAEKIIESAQQDQRRAEERSRLLTLM 300 EQARVDLQGQL +ARNQR L+AQT+K+AHDSRIEA+K IE++QQDQRRA+E S+LL L+ Sbjct: 241 EQARVDLQGQLDDARNQRATLVAQTRKDAHDSRIEADKTIEASQQDQRRADEHSKLLKLV 300 Query: 301 APVSGTVQQLAVHTVGGVVAAAQPLMQIVPEEGDIEIEAWLENKDVGFVRIGQEVEVKID 360 APV+GTVQQL VHT+GGVV AAQPLMQIVP++ IE+EA LENKDVGFV IGQ EVKID Sbjct: 301 APVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDVGFVEIGQAAEVKID 360 Query: 361 AFDYTKYGTVPARVVHVSRDAIQDEKKGLVYSARILLSRNSLNVDGRSLPLSAGLAVNVG 420 AFDYTKYGT+PARVVH+SRDAIQDEKKGL+YSARI+L ++SL VDGR + LSAG+A NV Sbjct: 361 AFDYTKYGTLPARVVHISRDAIQDEKKGLLYSARIVLDQSSLIVDGRKVQLSAGMAANVE 420 Query: 421 IRTGSRRVIEYVLSPLMRHQKEALHER 447 I+TG+RRVIEY LSPL+RHQKEALHER Sbjct: 421 IKTGNRRVIEYALSPLIRHQKEALHER 447 >ref|YP_902032.1| HlyD family type I secretion membrane fusion protein [Pelobacter propionicus DSM 2379] gb|ABK99974.1| type I secretion membrane fusion protein, HlyD family [Pelobacter propionicus DSM 2379] Length = 447 Score = 461 bits (1186), Expect = e-127, Method: Composition-based stats. Identities = 260/447 (58%), Positives = 330/447 (73%) Query: 1 MSLHHRSRAWLALWQRYGRVLRHAWRQRDPRKSGFYNEQEAEFLPAALSLQESPDSASLR 60 MS+ R A+ + R+ + H WR R + QEAEFLPAALSLQE P S R Sbjct: 1 MSIRQRMAAFGDVLSRFKTIFSHCWRNRHQLEGKVLAAQEAEFLPAALSLQEKPVSPVAR 60 Query: 61 WTARLLMAMVLLTLLWSLVGHIDIVVNATGKVIPSARIKTIASIEVASVRDILVREGQRV 120 A++LMA+V++ + WS+VG IDI+VNA+GK+IPS KTIA ++VASVR + V EGQ+V Sbjct: 61 LLAKVLMALVVVLITWSIVGKIDIIVNASGKIIPSGYTKTIACVDVASVRALYVAEGQQV 120 Query: 121 RVGQILMELDATSSDAERDKAADAVAQARLQVARAAAMMQAVQELQAPQLAEVGGAGRAQ 180 R G +L+ELDA +SDAERDKAA A + LQVA A +++ A+ P+L + Q Sbjct: 121 RAGDLLIELDAIASDAERDKAAGDRAVSILQVACARSLLAAIDGGSPPRLPPIATVPADQ 180 Query: 181 RESAERQLQGQYQDFRARLARIDDEITRYAAALQLASARATDYKELMADNTVSRHAWLEK 240 +A++QL QY D++ARL RID +I R++ ALQLA+ RATDYK L+ D VS HAWLEK Sbjct: 181 WLAAKQQLDAQYGDYQARLKRIDGDIERFSLALQLATQRATDYKALLKDRDVSYHAWLEK 240 Query: 241 EQARVDLQGQLSEARNQRTALLAQTQKEAHDSRIEAEKIIESAQQDQRRAEERSRLLTLM 300 EQARVDL GQL++ARNQR AL+A+T++ AH+ E K S+QQD RA SRLL L Sbjct: 241 EQARVDLAGQLADARNQRNALVAETRRVAHEQSTEGSKTAASSQQDAIRAAAHSRLLKLT 300 Query: 301 APVSGTVQQLAVHTVGGVVAAAQPLMQIVPEEGDIEIEAWLENKDVGFVRIGQEVEVKID 360 APVSGTVQQL VHT+GGVVAAA PLMQIVP E +E+EA++ENKDVGFV+ GQ EVKID Sbjct: 301 APVSGTVQQLNVHTIGGVVAAASPLMQIVPRESRVEVEAFMENKDVGFVQEGQSAEVKID 360 Query: 361 AFDYTKYGTVPARVVHVSRDAIQDEKKGLVYSARILLSRNSLNVDGRSLPLSAGLAVNVG 420 AF+YTKYGT+ RV HVSRDAI DEK+GL+YS +I L R+S+ VDGR +PLSAG+ VNV Sbjct: 361 AFEYTKYGTIKGRVTHVSRDAIADEKRGLIYSTKIRLDRSSITVDGREMPLSAGMTVNVE 420 Query: 421 IRTGSRRVIEYVLSPLMRHQKEALHER 447 I+TG RR+IEYVLSPL+RH++E+L+ER Sbjct: 421 IKTGDRRIIEYVLSPLLRHKRESLNER 447 >emb|CBJ39920.1| Type I secretion adaptor protein (HlyD family) [Ralstonia solanacearum CMR15] Length = 447 Score = 444 bits (1143), Expect = e-122, Method: Composition-based stats. Identities = 253/447 (56%), Positives = 331/447 (74%) Query: 1 MSLHHRSRAWLALWQRYGRVLRHAWRQRDPRKSGFYNEQEAEFLPAALSLQESPDSASLR 60 MSL HR AW LWQRY V RH W+QRD + EAEFLPAALSLQ P S + R Sbjct: 1 MSLRHRWEAWGELWQRYREVFRHYWKQRDALSLPRFEVHEAEFLPAALSLQAQPVSPAGR 60 Query: 61 WTARLLMAMVLLTLLWSLVGHIDIVVNATGKVIPSARIKTIASIEVASVRDILVREGQRV 120 W AR+LM ++++ LLWS++G +DI+VNATGK+IPS R KTIA++EVA+VR + V EGQ V Sbjct: 61 WVARILMLLIVVLLLWSILGKVDIIVNATGKIIPSERTKTIAAVEVATVRKLHVEEGQIV 120 Query: 121 RVGQILMELDATSSDAERDKAADAVAQARLQVARAAAMMQAVQELQAPQLAEVGGAGRAQ 180 + G +L+ELDA +SD+ERDKA ARLQ AR+ A+++A+ P L + G + Sbjct: 121 KAGDVLIELDARASDSERDKALGNEQLARLQAARSRALIEALDHGGTPHLPPLDGIPSVR 180 Query: 181 RESAERQLQGQYQDFRARLARIDDEITRYAAALQLASARATDYKELMADNTVSRHAWLEK 240 +E+ L Q++D+ A+L R+D EI RYA AL LA+ RA DY +L D+ VS+HA++EK Sbjct: 181 WNESEQHLVDQWRDYTAKLNRLDGEIRRYAEALPLATQRARDYADLAKDHDVSQHAYIEK 240 Query: 241 EQARVDLQGQLSEARNQRTALLAQTQKEAHDSRIEAEKIIESAQQDQRRAEERSRLLTLM 300 EQAR+D +GQL EAR+Q+TAL A+T++ A DS +A+KI+ + +D RRA S L L+ Sbjct: 241 EQARIDTEGQLREARDQKTALTAETRRVAQDSLTDAKKILGESSEDARRASVHSEQLKLV 300 Query: 301 APVSGTVQQLAVHTVGGVVAAAQPLMQIVPEEGDIEIEAWLENKDVGFVRIGQEVEVKID 360 APVSGTVQQL VHTVG V AQPLMQIVPE+ +E+EA+LENKDVGFV+ GQE +VKID Sbjct: 301 APVSGTVQQLTVHTVGTAVPVAQPLMQIVPEQSVVEVEAFLENKDVGFVQEGQEAQVKID 360 Query: 361 AFDYTKYGTVPARVVHVSRDAIQDEKKGLVYSARILLSRNSLNVDGRSLPLSAGLAVNVG 420 F+YTKYGT+PARV HVSRDAI+DEKKGL+YS + L + S++VDGR + LS G++ V Sbjct: 361 TFEYTKYGTIPARVTHVSRDAIEDEKKGLIYSVLVTLDKTSIDVDGRKIKLSPGMSTTVE 420 Query: 421 IRTGSRRVIEYVLSPLMRHQKEALHER 447 I+TG+RRVIEYVLSPL++H +E+L ER Sbjct: 421 IKTGTRRVIEYVLSPLIQHARESLRER 447 >ref|NP_521852.1| hemolysin secretion-like transmembrane protein [Ralstonia solanacearum GMI1000] emb|CAD17442.1| probable typeIsecretion system transmembrane protein [Ralstonia solanacearum GMI1000] Length = 447 Score = 440 bits (1132), Expect = e-121, Method: Composition-based stats. Identities = 251/447 (56%), Positives = 331/447 (74%) Query: 1 MSLHHRSRAWLALWQRYGRVLRHAWRQRDPRKSGFYNEQEAEFLPAALSLQESPDSASLR 60 MSL HR AW LWQRY V RH W+QRD + EAEFLPAALSLQ P S + R Sbjct: 1 MSLRHRWEAWSELWQRYRDVFRHYWKQRDALSLPRFEVHEAEFLPAALSLQAQPVSPAGR 60 Query: 61 WTARLLMAMVLLTLLWSLVGHIDIVVNATGKVIPSARIKTIASIEVASVRDILVREGQRV 120 W AR+LM ++++ LLWS++G +DI+VNATGK+IPS R KTIA++EVA+VR + V EGQ V Sbjct: 61 WVARILMLLIVVLLLWSILGKVDIIVNATGKIIPSERTKTIAAVEVATVRKLHVEEGQIV 120 Query: 121 RVGQILMELDATSSDAERDKAADAVAQARLQVARAAAMMQAVQELQAPQLAEVGGAGRAQ 180 + G +L+ELDA +SD+ERDKA ARLQ AR+ A++ A+ + P L + G + Sbjct: 121 KAGDVLIELDARASDSERDKAVGNEQLARLQAARSRALIGALDQGGTPHLPPLEGIPPER 180 Query: 181 RESAERQLQGQYQDFRARLARIDDEITRYAAALQLASARATDYKELMADNTVSRHAWLEK 240 AE+ L Q++D+ A+L R+D EI RYA AL LA+ RA DY +L D+ VS+HA+LEK Sbjct: 181 LHEAEQHLADQWRDYTAKLNRLDGEIRRYAEALPLATQRARDYADLAKDHDVSQHAYLEK 240 Query: 241 EQARVDLQGQLSEARNQRTALLAQTQKEAHDSRIEAEKIIESAQQDQRRAEERSRLLTLM 300 EQAR+D++GQL +AR+Q+ AL A+T++ A DS +A+K++ + +D RRA S L L+ Sbjct: 241 EQARIDIEGQLRDARDQKAALTAETRRVAQDSLTDAKKVLGESSEDARRASVHSEQLRLV 300 Query: 301 APVSGTVQQLAVHTVGGVVAAAQPLMQIVPEEGDIEIEAWLENKDVGFVRIGQEVEVKID 360 APVSGTVQQL VHTVG V AQPLMQIVPE+ +E+EA+LENKDVGFV+ GQ+ +VKID Sbjct: 301 APVSGTVQQLTVHTVGTAVPVAQPLMQIVPEQSVVEVEAFLENKDVGFVQEGQDAQVKID 360 Query: 361 AFDYTKYGTVPARVVHVSRDAIQDEKKGLVYSARILLSRNSLNVDGRSLPLSAGLAVNVG 420 F+YTKYGT+PARV HVSRDAI+DEKKGL+YS + L + S++VDGR + LS G++ V Sbjct: 361 TFEYTKYGTIPARVTHVSRDAIEDEKKGLIYSVMVTLDKTSIDVDGRKIKLSPGMSTTVE 420 Query: 421 IRTGSRRVIEYVLSPLMRHQKEALHER 447 I+TG+RRVIEYVLSPL++H +E+L ER Sbjct: 421 IKTGTRRVIEYVLSPLIQHARESLRER 447 >ref|YP_003747521.1| hemolysin D [Ralstonia solanacearum CFBP2957] emb|CBJ53104.1| Type I secretion adaptor protein (HlyD family) [Ralstonia solanacearum CFBP2957] Length = 447 Score = 440 bits (1131), Expect = e-121, Method: Composition-based stats. Identities = 250/447 (55%), Positives = 330/447 (73%) Query: 1 MSLHHRSRAWLALWQRYGRVLRHAWRQRDPRKSGFYNEQEAEFLPAALSLQESPDSASLR 60 MSL HR AW LWQRY V R+ W+QRD + EAEFLPAALSLQ P S R Sbjct: 1 MSLRHRWEAWSELWQRYRDVFRYYWKQRDALSLPRFEVHEAEFLPAALSLQAQPVSPVGR 60 Query: 61 WTARLLMAMVLLTLLWSLVGHIDIVVNATGKVIPSARIKTIASIEVASVRDILVREGQRV 120 W AR+L+ ++ + LLWS++G +DI+VNATGK+IPS R KTIA++EVA+VR + V EGQ V Sbjct: 61 WVARILILLIAVLLLWSILGKVDIIVNATGKIIPSERTKTIAAVEVATVRKLHVEEGQVV 120 Query: 121 RVGQILMELDATSSDAERDKAADAVAQARLQVARAAAMMQAVQELQAPQLAEVGGAGRAQ 180 + G +L+ELDA +SD+ERDKA ARLQ AR+ A++ A+ P L + G + Sbjct: 121 KAGDVLIELDARASDSERDKALGNEQLARLQAARSRALIDALDHGGTPHLPPLDGIPPVR 180 Query: 181 RESAERQLQGQYQDFRARLARIDDEITRYAAALQLASARATDYKELMADNTVSRHAWLEK 240 +E+ L Q++D+ A+L R+D EI RYA AL LA+ RA DY +L D+ VS+HA++EK Sbjct: 181 WNESEQHLVDQWRDYTAKLNRLDGEIRRYAEALPLATQRARDYADLAKDHDVSQHAYIEK 240 Query: 241 EQARVDLQGQLSEARNQRTALLAQTQKEAHDSRIEAEKIIESAQQDQRRAEERSRLLTLM 300 EQAR+D++GQL EAR+Q+TAL A+T++ A DS +A+KI+ + +D RRA S L L+ Sbjct: 241 EQARIDIEGQLREARDQKTALTAETRRVAQDSLTDAKKILGESSEDARRASVHSEQLKLV 300 Query: 301 APVSGTVQQLAVHTVGGVVAAAQPLMQIVPEEGDIEIEAWLENKDVGFVRIGQEVEVKID 360 APVSGTVQQL VHTVG V AQ LMQIVPE+ +E+EA++ENKDVGFV+ GQE +VKID Sbjct: 301 APVSGTVQQLTVHTVGTAVPVAQALMQIVPEQSVVEVEAFMENKDVGFVQEGQEAQVKID 360 Query: 361 AFDYTKYGTVPARVVHVSRDAIQDEKKGLVYSARILLSRNSLNVDGRSLPLSAGLAVNVG 420 F+YTKYGT+PARV HVSRDAI+DEKKGL+YS ++ L R S++VDGR + LS G++ +V Sbjct: 361 TFEYTKYGTIPARVTHVSRDAIEDEKKGLIYSVKVALDRTSIDVDGRKIKLSPGMSTSVE 420 Query: 421 IRTGSRRVIEYVLSPLMRHQKEALHER 447 I+TG+RRVIEYVLSPL++H +E+L ER Sbjct: 421 IKTGTRRVIEYVLSPLIQHARESLRER 447