BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.3245.p (193 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777806.1| small-conductance mechanosensitive channel p... 297 6e-79 ref|YP_001899317.1| small-conductance mechanosensitive channel-l... 240 9e-62 ref|YP_004127770.1| mscs mechanosensitive ion channel [Alicyclip... 187 7e-46 ref|YP_004146641.1| mechanosensitive ion channel MscS [Pseudoxan... 171 5e-41 ref|YP_931556.1| hypothetical protein azo0051 [Azoarcus sp. BH72... 170 9e-41 >ref|YP_003777806.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] gb|ADJ65898.1| small-conductance mechanosensitive channel protein [Herbaspirillum seropedicae SmR1] Length = 193 Score = 297 bits (760), Expect = 6e-79, Method: Composition-based stats. Identities = 165/193 (85%), Positives = 180/193 (93%) Query: 1 MNKFLQSIHDGLPQWMADWFDVLVPAVEVALIMIGALAIMRIAKLLIGRVGRTYGLPAKA 60 M+KFLQSIHDGLPQW+ADWFDVLVP EV LI++GALAIMR+A++LIGR+ TYG P KA Sbjct: 1 MSKFLQSIHDGLPQWLADWFDVLVPTAEVILIVLGALAIMRVARMLIGRLALTYGFPNKA 60 Query: 61 AALTQRVLGFFVYGGAILWALERLGVSGAVLWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 AALTQRVLGFFVYGGA+LWALER+GVS A LWSAFTGFAAVGAVAFFAAWSVLSNLFCAI Sbjct: 61 AALTQRVLGFFVYGGALLWALERMGVSSAALWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 Query: 121 LIYTTQPFRVGDVIEVIEGGDKPGVKGRVLDINLVYTTLSEESQGAGTTLQLPNSLFFQR 180 LIYTTQPFRVGDVIEV+EGGDKPG+KGRVLDINLVYTTL+EE +G TTLQLPNSLFFQR Sbjct: 121 LIYTTQPFRVGDVIEVLEGGDKPGLKGRVLDINLVYTTLAEEHEGGETTLQLPNSLFFQR 180 Query: 181 IVRRWNDGSSSLF 193 IVRRW+DGS SLF Sbjct: 181 IVRRWSDGSGSLF 193 >ref|YP_001899317.1| small-conductance mechanosensitive channel-like protein [Ralstonia pickettii 12J] gb|ACD26885.1| small-conductance mechanosensitive channel-like protein [Ralstonia pickettii 12J] Length = 187 Score = 240 bits (612), Expect = 9e-62, Method: Composition-based stats. Identities = 124/186 (66%), Positives = 150/186 (80%), Gaps = 1/186 (0%) Query: 1 MNKFLQSIHDGLPQWMADWFDVLVPAVEVALIMIGALAIMRIAKLLIGRVGRTYGLPAKA 60 MNK LQ+IH+ LPQW+ADWFD+LVPA+EV LI GA +RIA+ L+ ++ TY LP K Sbjct: 1 MNKLLQAIHESLPQWLADWFDILVPAIEVVLIAAGAWLAVRIARRLLRKLTNTYSLPGKV 60 Query: 61 AALTQRVLGFFVYGGAILWALERLGVSGAVLWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 AAL+ RV+ VY A LWALER+GVSG VLW+AFTGFA VGAVAFFAAWSVLSNLFCA+ Sbjct: 61 AALSLRVVATIVYVAATLWALERMGVSGTVLWTAFTGFATVGAVAFFAAWSVLSNLFCAL 120 Query: 121 LIYTTQPFRVGDVIEVIEGGDKPGVKGRVLDINLVYTTLSEES-QGAGTTLQLPNSLFFQ 179 LIY T FR+GDV+E++E G+KPG+KGRVLD+NLVYTTL E + +G LQLPNSL FQ Sbjct: 121 LIYVTGAFRIGDVVELLENGEKPGIKGRVLDVNLVYTTLLETTVEGQEVVLQLPNSLVFQ 180 Query: 180 RIVRRW 185 R++RRW Sbjct: 181 RVLRRW 186 >ref|YP_004127770.1| mscs mechanosensitive ion channel [Alicycliphilus denitrificans BC] ref|YP_004387206.1| MscS Mechanosensitive ion channel [Alicycliphilus denitrificans K601] gb|ADV00883.1| MscS Mechanosensitive ion channel [Alicycliphilus denitrificans BC] gb|AEB83690.1| MscS Mechanosensitive ion channel [Alicycliphilus denitrificans K601] Length = 220 Score = 187 bits (475), Expect = 7e-46, Method: Composition-based stats. Identities = 105/189 (55%), Positives = 131/189 (69%), Gaps = 1/189 (0%) Query: 1 MNKFLQSIHDGLPQWMADWFDVLVPAVEVALIMIGALAIMRIAKLLIGRVGRTYGLPAKA 60 M+ L+ + LP W DW DV+VP +++ALI++ AL + I + LI R Y P + Sbjct: 1 MSPMLERLKTILPPWAHDWLDVIVPGMQIALIVLAALLLHYIVRRLIRRASDHYQFPHEL 60 Query: 61 AALTQRVLGFFVYGGAILWALERLGVSGAVLWSAFTGFAAVGAVAFFAAWSVLSNLFCAI 120 V+ + + GGA L ALERLGVS AVLW+AFTGFA VGAVAFFAAWSVLSNLFCA Sbjct: 61 LTPVNAVVRWLILGGAALLALERLGVSAAVLWTAFTGFATVGAVAFFAAWSVLSNLFCAF 120 Query: 121 LIYTTQPFRVGDVIEVIEGGDKPGVKGRVLDINLVYTTLSEESQ-GAGTTLQLPNSLFFQ 179 LI T PFRVGD IE+++ +KPG GRVLDINL+YTTL + + GT LQ+PN+L FQ Sbjct: 121 LILTVGPFRVGDHIELLDTAEKPGALGRVLDINLLYTTLEDATAPEPGTLLQIPNALIFQ 180 Query: 180 RIVRRWNDG 188 R+VRRW G Sbjct: 181 RVVRRWRAG 189 >ref|YP_004146641.1| mechanosensitive ion channel MscS [Pseudoxanthomonas suwonensis 11-1] gb|ADV27410.1| MscS Mechanosensitive ion channel [Pseudoxanthomonas suwonensis 11-1] Length = 204 Score = 171 bits (433), Expect = 5e-41, Method: Composition-based stats. Identities = 98/177 (55%), Positives = 131/177 (74%), Gaps = 3/177 (1%) Query: 12 LPQWMADWFDVLVPAVEVALIMIGALAIMRIAKLLIGRVGRTYGLPAKAAALTQRVLGFF 71 LP+W+ W +VP + LI++ A + ++ + L+ R+ GLP + + RV Sbjct: 5 LPEWLQGWLGTIVPIGQALLIVLAAWLLQQLVRRLVRRLMDRRGLPPEMGVVLHRVSAAL 64 Query: 72 VYGGAILWALERLGVSGAVLWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIYTTQPFRVG 131 + GA+L LERLGVSG VLW+AFT FAAVGAVAFFAAWSVLSN+FC +LI+TT+PFR+ Sbjct: 65 IGFGALLMVLERLGVSGTVLWTAFTSFAAVGAVAFFAAWSVLSNIFCTVLIFTTRPFRLY 124 Query: 132 DVIEVIEGGDKPGVKGRVLDINLVYTTLSE---ESQGAGTTLQLPNSLFFQRIVRRW 185 D IEV+E G+KPG+KGRV+DINL+YTTL E + + AG++LQ+PNSLFFQR+VRRW Sbjct: 125 DYIEVVENGEKPGLKGRVVDINLIYTTLQEVPRDPEHAGSSLQVPNSLFFQRVVRRW 181 >ref|YP_931556.1| hypothetical protein azo0051 [Azoarcus sp. BH72] emb|CAL92669.1| conserved hypothetical membrane protein [Azoarcus sp. BH72] Length = 250 Score = 170 bits (431), Expect = 9e-41, Method: Composition-based stats. Identities = 96/175 (54%), Positives = 129/175 (73%), Gaps = 1/175 (0%) Query: 12 LPQWMADWFDVLVPAVEVALIMIGALAIMRIAKLLIGRVGRTYGLPAKAAALTQRVLGFF 71 LP W W D++ +++ALI+ GA + + + L+ R+G Y LP + +R + F Sbjct: 71 LPAWADPWLDLIHLGLQIALILFGAALLRMVLRRLLRRLGEHYNLPPELVIGARRTISFV 130 Query: 72 VYGGAILWALERLGVSGAVLWSAFTGFAAVGAVAFFAAWSVLSNLFCAILIYTTQPFRVG 131 +Y GA+L L+R GVSG VLW+AFTGFAAV AVAFFAAWSVLSN+FC +LI+TT+PFR+ Sbjct: 131 IYSGALLLVLDRFGVSGTVLWTAFTGFAAVAAVAFFAAWSVLSNIFCTLLIFTTRPFRLY 190 Query: 132 DVIEVIEGGDKPGVKGRVLDINLVYTTLSEESQGAG-TTLQLPNSLFFQRIVRRW 185 D IE++E G+KPG+KG+V+DINL+YTTL E + G T LQ+PNSLFFQR +RRW Sbjct: 191 DHIELLENGEKPGLKGQVVDINLIYTTLQESAGEHGDTVLQVPNSLFFQRCLRRW 245