BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.3237.p (494 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_08261973.1| cation efflux system protein cusB [Asticcacau... 468 e-129 ref|YP_001187703.1| RND family efflux transporter MFP subunit [P... 456 e-126 ref|ZP_08142800.1| RND family efflux transporter MFP subunit [Ps... 455 e-126 ref|ZP_03399920.1| Secretion protein HlyD [Pseudomonas syringae ... 452 e-125 ref|YP_003543970.1| copper efflux system membrane protein CusB [... 450 e-124 >ref|ZP_08261973.1| cation efflux system protein cusB [Asticcacaulis biprosthecum C19] gb|EGF93775.1| cation efflux system protein cusB [Asticcacaulis biprosthecum C19] Length = 493 Score = 468 bits (1204), Expect = e-129, Method: Composition-based stats. Identities = 250/488 (51%), Positives = 342/488 (70%), Gaps = 11/488 (2%) Query: 6 YQIAMLILAVGAGSGMAGYWVAQRAAGAQAMQVEPAKATTHVERKVLYWYDPMMPNQRFD 65 Y +A+ +++ G G G++ R ++A+ + RKVLYWYDPM+P Q F+ Sbjct: 15 YGVAIALVSAGTGYGIS------RLNTSKAL----TSTSEASGRKVLYWYDPMVPGQHFE 64 Query: 66 KPGKSPFMDMQLIAKYADEQGDGAGVKIDSGISQNLGMRTAKVERGSFLSTVRAVANVQL 125 PGKSPFMDM L+ KYADE+ GVKID G QNLG+R A+VER F + V+ Sbjct: 65 NPGKSPFMDMDLVPKYADEEAKAGGVKIDPGRLQNLGIRLAQVERTDFALDLTVAGTVEF 124 Query: 126 NDRQVAIVQARSNGFVERVYARAPGDVIARGAPLVDLLIPEWAGAQAEFLAVLGTKDLAL 185 N R VAIVQAR++GFV+RVYARAPGD++ GAPL D+LIP WA AQ+E+LAV T D AL Sbjct: 125 NQRDVAIVQARADGFVQRVYARAPGDIVGAGAPLADILIPSWAAAQSEYLAVRRTADPAL 184 Query: 186 IQAARTRLQLLGMSGDLISRVEKSGRPQTVVTIAAPISGLIQTLDIRSGMTVSAGAPLAR 245 +AAR RL LLGMS LI +V +SG+P T+VTI+ P+ G IQTLD+R GMTV++G LA+ Sbjct: 185 ERAARQRLTLLGMSQGLIDQVSRSGKPNTIVTISTPVGGAIQTLDVRQGMTVNSGQTLAQ 244 Query: 246 INGLDTVWLEAAIPEAQAALVNVGGKLEADFAAYSGEKFTGKILAVLPESNADSRTLRVR 305 ++GL +VWL AA+PEA A+ V++G ++A AA+ GE F+GK+ A+LP + +D+RTL VR Sbjct: 245 VSGLSSVWLNAAVPEALASQVSLGQPVKAKLAAFPGESFSGKVSAILPTAQSDTRTLSVR 304 Query: 306 AELPNRDGKLKPGMYAQIRLGGGKAAPSLLVPSEAVIRTGARNILLVTTGDGRYQPVEVK 365 EL NR+G+L+PGMYA + G + +L VPSEAVIRTG R I++++ GDGR++PVE++ Sbjct: 305 VELANRNGRLRPGMYATVHFPGNTST-ALSVPSEAVIRTGKRTIVMLSKGDGRFEPVEIQ 363 Query: 366 LGQESDGKSIILDGVTEGQQVVTSGQFLIDSEASLKGVLARLNTSAGADAKSAQMPLNEA 425 +G+ES+ ++ +L G+TEG+ VV SGQFL+DSEA+L G+ AR + A + A Sbjct: 364 VGRESENRTEVLAGLTEGENVVASGQFLVDSEANLSGISARAIQDREGVGQEATAKVFTA 423 Query: 426 TGKIESLRGTDITLSHGPVPTLGWGAMTMSFKLARPEMATGLKTGDSVHFGFQERNGDYV 485 G+IE+L +TLSHGPVP L W AMTM+FKLA P +ATGLK GD V F F ++ + Sbjct: 424 QGRIETLTTEQVTLSHGPVPALSWPAMTMTFKLASPSIATGLKVGDQVDFSFAQKAEGPI 483 Query: 486 IEKLDRST 493 +E + + T Sbjct: 484 VETMRKGT 491 >ref|YP_001187703.1| RND family efflux transporter MFP subunit [Pseudomonas mendocina ymp] gb|ABP84971.1| efflux transporter, RND family, MFP subunit [Pseudomonas mendocina ymp] Length = 486 Score = 456 bits (1172), Expect = e-126, Method: Composition-based stats. Identities = 253/491 (51%), Positives = 330/491 (67%), Gaps = 10/491 (2%) Query: 1 MKKSTYQIAMLILAVGAGSGMAGYWVAQRAAGAQAMQVEPAKATTHVERKVLYWYDPMMP 60 MK S + +L +G + GYW+A+R A + M P A T ERKVLYWYDPM P Sbjct: 1 MKISALGFSAALLTMGIAAAGGGYWLAERPAKHEVM---PTPAPTD-ERKVLYWYDPMQP 56 Query: 61 NQRFDKPGKSPFMDMQLIAKYADEQGDGAGVKIDSGISQNLGMRTAKVERGSFLSTVRAV 120 +QRFDKPGKSPFMDM+L+ KYAD D + + + S QNLGMRTA V RG S + AV Sbjct: 57 DQRFDKPGKSPFMDMELVPKYADVGQDSSTLSVPSQAVQNLGMRTAPVTRGVLPSGIEAV 116 Query: 121 ANVQLNDRQVAIVQARSNGFVERVYARAPGDVIARGAPLVDLLIPEWAGAQAEFLAVLGT 180 ++ N R+VA QAR+ GFVERVY APGDV+ G PLVDLLIPEW+ AQ EFLAVL T Sbjct: 117 GSLAYNQREVANQQARAGGFVERVYGHAPGDVLPAGTPLVDLLIPEWSAAQLEFLAVLRT 176 Query: 181 KDLALIQAARTRLQLLGMSGDLISRVEKSGRPQTVVTIAAPISGLIQTLDIRSGMTVSAG 240 D LI A + RL+LLGM L+ +V +SG+P+ V TI PI+G +Q L++R+GMTV+AG Sbjct: 177 ADTRLISATQERLRLLGMPQSLVEQVRRSGQPRAVQTITTPIAGELQALEVRTGMTVAAG 236 Query: 241 APLARINGLDTVWLEAAIPEAQAALVNVGGKLEADFAAYSGEKFTGKILAVLPESNADSR 300 LARINGL TVWL+AAIPE QA V +G + A A+ + G+++A+LP ++ +R Sbjct: 237 QDLARINGLSTVWLDAAIPEDQAGAVQLGASITATLTAFPEQPLQGRVVALLPSADPQTR 296 Query: 301 TLRVRAELPNRDGKLKPGMYAQIRLGGGKAAPSLLVPSEAVIRTGARNILLVTTGDGRYQ 360 TL VR+ELPN GKL+PGM+A +RL +LLVPSEAVIRTG R ++++ GDGRY+ Sbjct: 297 TLTVRSELPNPAGKLRPGMFAAVRLNSAVEQSTLLVPSEAVIRTGKRALVMLAEGDGRYR 356 Query: 361 PVEVKLGQESDGKSIILDGVTEGQQVVTSGQFLIDSEASLKGVLARLNTSAGADAKSAQM 420 P E+ LG+E+DG+ + G+ EGQ VVTSGQFLIDSEASL+GV+A A Sbjct: 357 PQEITLGREADGRLEVFSGLEEGQAVVTSGQFLIDSEASLQGVVASHAKQDNAKK----- 411 Query: 421 PLNEATGKIESLRGTDITLSHGPVPTLGWGAMTMSFKLARPEMATGLKTGDSVHFGFQER 480 L+E+ G I +L ++TL HGP +L MTM+F LA PE+A GLK GD V G ++ Sbjct: 412 -LHESHGVIRALDAEEVTLEHGPFESLNMPGMTMAFPLASPEVAAGLKPGDHVRIGARQT 470 Query: 481 NGDYVIEKLDR 491 + +E+LD+ Sbjct: 471 DSGLEVERLDK 481 >ref|ZP_08142800.1| RND family efflux transporter MFP subunit [Pseudomonas sp. TJI-51] ref|YP_004380294.1| RND family efflux transporter MFP subunit [Pseudomonas mendocina NK-01] gb|EGB95931.1| RND family efflux transporter MFP subunit [Pseudomonas sp. TJI-51] gb|AEB58542.1| RND family efflux transporter MFP subunit [Pseudomonas mendocina NK-01] Length = 486 Score = 455 bits (1170), Expect = e-126, Method: Composition-based stats. Identities = 252/491 (51%), Positives = 332/491 (67%), Gaps = 10/491 (2%) Query: 1 MKKSTYQIAMLILAVGAGSGMAGYWVAQRAAGAQAMQVEPAKATTHVERKVLYWYDPMMP 60 MK ST+ A+ +L VG G+ GYW+AQR A + + P+ A T ERKVLYWYDPM P Sbjct: 1 MKISTFGFAVAVLTVGIGAAGGGYWLAQRQAKHELL---PSSAPTD-ERKVLYWYDPMQP 56 Query: 61 NQRFDKPGKSPFMDMQLIAKYADEQGDGAGVKIDSGISQNLGMRTAKVERGSFLSTVRAV 120 QRFDKPGKSPFMDM+L+ KYA + D + + + + QNLGMRT+ V RG S + V Sbjct: 57 EQRFDKPGKSPFMDMELVPKYAGSEQDPSVLSVPAQAVQNLGMRTSTVIRGVLPSDIEVV 116 Query: 121 ANVQLNDRQVAIVQARSNGFVERVYARAPGDVIARGAPLVDLLIPEWAGAQAEFLAVLGT 180 ++ N R+VA +QAR+ GFVERVY RAPGDV+ G PLVDLLIPEW+ AQ EFLAVL T Sbjct: 117 GSLAYNQREVANLQARAGGFVERVYGRAPGDVLPAGTPLVDLLIPEWSAAQLEFLAVLRT 176 Query: 181 KDLALIQAARTRLQLLGMSGDLISRVEKSGRPQTVVTIAAPISGLIQTLDIRSGMTVSAG 240 D LI AA+ RL+LLGM LI +V +SG+P+ V T+ PISG +Q L +R+GMTV AG Sbjct: 177 GDARLITAAQERLRLLGMPQALIEQVRRSGKPRAVQTLTTPISGELQALQVRAGMTVEAG 236 Query: 241 APLARINGLDTVWLEAAIPEAQAALVNVGGKLEADFAAYSGEKFTGKILAVLPESNADSR 300 LA INGL +VWL+AAIPEA A + VG ++ A+ A+ G+++A+LP ++ +R Sbjct: 237 QDLALINGLSSVWLDAAIPEAMAGSIQVGDEIRANLTAFPDRPLLGRVIALLPSADPQTR 296 Query: 301 TLRVRAELPNRDGKLKPGMYAQIRLGGGKAAPSLLVPSEAVIRTGARNILLVTTGDGRYQ 360 TL VR+ELPN GKL+PGM+A +RL +LLVPSEAVIRTG R ++++ GDGRY+ Sbjct: 297 TLTVRSELPNPAGKLRPGMFAAVRLNSAVEQSTLLVPSEAVIRTGKRALVMLAEGDGRYR 356 Query: 361 PVEVKLGQESDGKSIILDGVTEGQQVVTSGQFLIDSEASLKGVLARLNTSAGADAKSAQM 420 P E+ LG+E+DG+ + G+ EGQ VVTSGQFLIDSEASL+G+L AG + + Sbjct: 357 PQEITLGREADGRLEVFSGLEEGQAVVTSGQFLIDSEASLQGIL------AGNAEQDSTE 410 Query: 421 PLNEATGKIESLRGTDITLSHGPVPTLGWGAMTMSFKLARPEMATGLKTGDSVHFGFQER 480 L+ + G I +L G +TL HGP +L MTM+F L E+A GLK GD V ++ Sbjct: 411 GLHVSHGVIRALDGKQVTLEHGPFESLNMPGMTMAFPLVSSEVAVGLKPGDHVRIAARQT 470 Query: 481 NGDYVIEKLDR 491 N +IE+L + Sbjct: 471 NSGLLIEQLRK 481 >ref|ZP_03399920.1| Secretion protein HlyD [Pseudomonas syringae pv. tomato T1] ref|ZP_07230936.1| secretion protein HlyD [Pseudomonas syringae pv. tomato Max13] ref|ZP_07250586.1| secretion protein HlyD [Pseudomonas syringae pv. tomato K40] gb|EEB57024.1| Secretion protein HlyD [Pseudomonas syringae pv. tomato T1] Length = 494 Score = 452 bits (1163), Expect = e-125, Method: Composition-based stats. Identities = 246/484 (50%), Positives = 328/484 (67%), Gaps = 5/484 (1%) Query: 11 LILAVGAGSGMAG-YWVA-QRAAGAQAMQVEPAKATTHVERKVLYWYDPMMPNQRFDKPG 68 L++ V G+AG YW A QR +G E + ++ ERK LYWYDPM P Q+FDKPG Sbjct: 10 LLVGVSLAFGVAGGYWFAHQRVSGVPGASPEQSPNSSE-ERKALYWYDPMYPQQKFDKPG 68 Query: 69 KSPFMDMQLIAKYADEQGDGAGVKIDSGISQNLGMRTAKVERGSFLSTVRAVANVQLNDR 128 KSPFMDMQL+ YA GD A V ID ++QNLG+R A V RG F S++ + N+R Sbjct: 69 KSPFMDMQLVPLYAGGAGDRATVSIDPSLTQNLGLRFATVTRGIFDSSLDVTGVLAFNER 128 Query: 129 QVAIVQARSNGFVERVYARAPGDVIARGAPLVDLLIPEWAGAQAEFLAVLGTKDLALIQA 188 VA++QAR+ GFVERVYA APGDV+ A L D+L+PEWA AQ EFLA+ D L+ A Sbjct: 129 DVAVIQARTPGFVERVYAHAPGDVLKANAALADILVPEWAAAQTEFLAIKHNGDADLLAA 188 Query: 189 ARTRLQLLGMSGDLISRVEKSGRPQTVVTIAAPISGLIQTLDIRSGMTVSAGAPLARING 248 AR RL+L GM LI++VE+SG+ Q +T+ +PISG++Q L++R+GMTVSAG LAR+NG Sbjct: 189 ARQRLRLTGMPAALITQVERSGKVQPNLTLTSPISGVLQELNVRAGMTVSAGDTLARVNG 248 Query: 249 LDTVWLEAAIPEAQAALVNVGGKLEADFAAYSGEKFTGKILAVLPESNADSRTLRVRAEL 308 L +VWL A+PE+ + + VG +EA A+ G +G + A+LPE+N DSRTLRVR EL Sbjct: 249 LSSVWLAVAVPESDSGAITVGQAVEARLPAFPGTTLSGNVSAILPETNPDSRTLRVRVEL 308 Query: 309 PNRDGKLKPGMYAQIRLGGGKAAPSLLVPSEAVIRTGARNILLVTTGDGRYQPVEVKLGQ 368 PN DG+L+PG+ AQ+RL L VPSEAVIRTG R ++++ GRY+PVEV LGQ Sbjct: 309 PNPDGRLRPGLTAQVRLNRSTEQSVLWVPSEAVIRTGRRALVMLAEDAGRYRPVEVLLGQ 368 Query: 369 ESDGKSIILDGVTEGQQVVTSGQFLIDSEASLKGVLARLNTSAGADAKSAQMPLNEATGK 428 ESDGK+ I+ G+ GQ+VVTSGQFL+DSEASLKG++AR + + ++ +EA G+ Sbjct: 369 ESDGKTAIVKGLEAGQKVVTSGQFLLDSEASLKGIVARTDEESAPSETASS--FHEADGQ 426 Query: 429 IESLRGTDITLSHGPVPTLGWGAMTMSFKLARPEMATGLKTGDSVHFGFQERNGDYVIEK 488 I + ++TL+HGP TLG MTM+F LA P + GLK GD V + + +E+ Sbjct: 427 IVEINDKEVTLAHGPFKTLGMPGMTMTFPLASPALMQGLKAGDKVRVAVSQTDDGLRVER 486 Query: 489 LDRS 492 LD+S Sbjct: 487 LDKS 490 >ref|YP_003543970.1| copper efflux system membrane protein CusB [Sphingobium japonicum UT26S] ref|ZP_08389842.1| efflux transporter, RND family, MFP subunit [Sphingomonas sp. S17] ref|YP_004556173.1| efflux transporter, RND family, MFP subunit [Sphingobium chlorophenolicum L-1] ref|YP_004831172.1| copper efflux system membrane protein CusB [Sphingobium sp. SYK-6] dbj|BAI95358.1| copper efflux system membrane protein CusB [Sphingobium japonicum UT26S] gb|EGI53901.1| efflux transporter, RND family, MFP subunit [Sphingomonas sp. S17] gb|AEG51667.1| efflux transporter, RND family, MFP subunit [Sphingobium chlorophenolicum L-1] dbj|BAK68640.1| copper efflux system membrane protein CusB [Sphingobium sp. SYK-6] Length = 496 Score = 450 bits (1158), Expect = e-124, Method: Composition-based stats. Identities = 258/483 (53%), Positives = 324/483 (67%), Gaps = 13/483 (2%) Query: 1 MKKSTYQIAMLILAVGAGSGMAGYWVAQRAAGAQAMQVEPAKATTHVERKVLYWYDPMMP 60 + KS + ILAV +G GYW R A Q+ PA A KVLYWYDPM P Sbjct: 3 LDKSALRWGASILAVALLAGGTGYWAGHRQA-PQSEATTPAGAG-----KVLYWYDPMFP 56 Query: 61 NQRFDKPGKSPFMDMQLIAKYADEQGDGAG--VKIDSGISQNLGMRTAKVERGSFLSTVR 118 NQ+FDKPGKSPFMDMQL+ +YAD GA V +D Q+LG+R + GS S + Sbjct: 57 NQKFDKPGKSPFMDMQLVPRYADGGSAGAAPTVAVDPAARQSLGLRVVAAKMGSLASALE 116 Query: 119 AVANVQLNDRQVAIVQARSNGFVERVYARAPGDVIARGAPLVDLLIPEWAGAQAEFLAVL 178 + N R VA++QARS GFV RVYARAPGDV+ GAP+ DLL+PEW GAQ E+L+V Sbjct: 117 VTGTIDFNQRDVAVIQARSGGFVSRVYARAPGDVVRAGAPIADLLLPEWGGAQTEYLSVR 176 Query: 179 GTKDLALIQAARTRLQLLGMSGDLISRVEKSGRPQTVVTIAAPISGLIQTLDIRSGMTVS 238 L AAR RL+L+GMS LI+ VE+SGRP VVTI PISG IQTLD R+G+T++ Sbjct: 177 RLGKPDLTAAARQRLRLMGMSDGLIASVERSGRPNGVVTITTPISGTIQTLDARAGVTLA 236 Query: 239 AGAPLARINGLDTVWLEAAIPEAQAALVNVGGKLEADFAAYSGEKFTGKILAVLPESNAD 298 G LA+++GL TVWL AA+PEA+A V VG A + GE+F G+++A+LP + AD Sbjct: 237 MGQTLAQVSGLGTVWLNAAVPEARAGDVRVGQNASTTLAGFPGERFAGRVIAILPTTQAD 296 Query: 299 SRTLRVRAELPNRDGKLKPGMYAQIRLGGGKAAPSLLVPSEAVIRTGARNILLVTTGDGR 358 SRTL VR ELPNRDG+L+PGM+A + L GG A P+LLVPSEAVIRTG R ++++ GDGR Sbjct: 297 SRTLTVRIELPNRDGRLRPGMFASVAL-GGDAKPALLVPSEAVIRTGRRTLVMLAAGDGR 355 Query: 359 YQPVEVKLGQESDGKSIILDGVTEGQQVVTSGQFLIDSEASLKGVLARL----NTSAGAD 414 Y P EV++G+E+ G++ IL G++ G+ VV SGQFLIDSEASL G+ AR SA Sbjct: 356 YHPAEVRIGREAGGETEILAGLSPGENVVVSGQFLIDSEASLSGIEARPIGGGAASATIA 415 Query: 415 AKSAQMPLNEATGKIESLRGTDITLSHGPVPTLGWGAMTMSFKLARPEMATGLKTGDSVH 474 A +++ L E TGKIE + +TLSH PVP L W AMTM+F LA P +A G K GD V Sbjct: 416 APASKATLYETTGKIERITANSVTLSHEPVPALDWPAMTMTFALANPGIARGFKAGDRVR 475 Query: 475 FGF 477 FGF Sbjct: 476 FGF 478