BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.3105.p (531 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774876.1| gamma-glutamate-cysteine ligase [Herbaspiril... 987 0.0 ref|YP_004751565.1| glutamate--cysteine ligase [Collimonas fungi... 658 0.0 ref|YP_001352856.1| glutamate--cysteine ligase [Janthinobacteriu... 656 0.0 ref|YP_002797731.1| glutamate--cysteine ligase [Azotobacter vine... 640 0.0 ref|YP_793675.1| glutamate--cysteine ligase [Pseudomonas aerugin... 621 e-176 >ref|YP_003774876.1| gamma-glutamate-cysteine ligase [Herbaspirillum seropedicae SmR1] gb|ADJ62968.1| gamma-glutamate-cysteine ligase protein [Herbaspirillum seropedicae SmR1] Length = 531 Score = 987 bits (2552), Expect = 0.0, Method: Composition-based stats. Identities = 487/531 (91%), Positives = 508/531 (95%) Query: 1 MTDLLTRRLQQLSQPHHLALLGQGLRGVERETLRVDGQGHLARTPHPPALGSALTNDLIT 60 MTDLL RRLQQLSQP HLALLGQGLRG+ERETLRVD QGHLARTPHPPALGSALTN+LIT Sbjct: 1 MTDLLNRRLQQLSQPQHLALLGQGLRGIERETLRVDAQGHLARTPHPPALGSALTNELIT 60 Query: 61 TDYSESLLEFITPAMPDIADALQRLDEIHRFAYTKLDGEFLWNASMPCVLPPEDEIPLAW 120 TDYSE+LLEFITPAMPDIADALQRLDEIHRFAY KL+GEFLWNASMPCVLPPEDEIP+AW Sbjct: 61 TDYSEALLEFITPAMPDIADALQRLDEIHRFAYAKLEGEFLWNASMPCVLPPEDEIPIAW 120 Query: 121 YGSSHIGMLKHVYRRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLRDGDDTVDPSLSRRD 180 YG+SHIGMLKHVYRRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLR+GDD+VD SLSRRD Sbjct: 121 YGNSHIGMLKHVYRRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLREGDDSVDLSLSRRD 180 Query: 181 YQSERYIALIRNFRRYSWLLMYLFGASPAVATNFLRGRPHQLETLSDDTLFLPYGTSLRM 240 YQSERYIALIRNFRRYSWLLMYLFGASPAVATNFLRGRPHQLETLS+DTL+LPYGTSLRM Sbjct: 181 YQSERYIALIRNFRRYSWLLMYLFGASPAVATNFLRGRPHQLETLSEDTLYLPYGTSLRM 240 Query: 241 SDLGYQNHAQANITPHYNNLPAYIRSLAHAVSEPYPPYEAIGTKRDGEWVQINTNILQIE 300 SDLGYQNHAQANITPHYNNLPAYIRSL+HAVSEPYPPYEAIGTKRDG+WVQINTNILQIE Sbjct: 241 SDLGYQNHAQANITPHYNNLPAYIRSLSHAVSEPYPPYEAIGTKRDGQWVQINTNILQIE 300 Query: 301 NEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEAARFLDVFLHF 360 NEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEAARFLDVFLHF Sbjct: 301 NEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEAARFLDVFLHF 360 Query: 361 LALESSCLTSDEEGVENRANFLATVKEGRRPGLKLQFKGESLLLTDWGQQLLDRMEPVAD 420 LALESSCLTSDEEG ENRANFLATVKEGRRPGL+L FKG S+ L WG+QLLDRM PVAD Sbjct: 361 LALESSCLTSDEEGAENRANFLATVKEGRRPGLQLHFKGASIGLKAWGEQLLDRMAPVAD 420 Query: 421 LLDRHLGGTQHRDSLAVQRAKLADPDSTPSARVLREIREAGNSFPAYTLANSQRQAAELI 480 LLDR GG+QHRDSL QRAKLADPDSTPSARVLREIR +GNSF YTLA+SQRQAAEL+ Sbjct: 421 LLDRQRGGSQHRDSLQAQRAKLADPDSTPSARVLREIRASGNSFADYTLASSQRQAAELM 480 Query: 481 ARPLSSSKTADFVAIAQASLDAQRDIERTQVGDFDTFVAAYRASTLGNIAI 531 ARPL ++ TADFVAIAQASLDAQR IERTQ GDFDTFVAAYRASTLGNIAI Sbjct: 481 ARPLPAATTADFVAIAQASLDAQRAIERTQEGDFDTFVAAYRASTLGNIAI 531 >ref|YP_004751565.1| glutamate--cysteine ligase [Collimonas fungivorans Ter331] gb|AEK60742.1| Glutamate--cysteine ligase [Collimonas fungivorans Ter331] Length = 577 Score = 658 bits (1697), Expect = 0.0, Method: Composition-based stats. Identities = 334/531 (62%), Positives = 397/531 (74%), Gaps = 3/531 (0%) Query: 1 MTDLLTRRLQQLSQPHHLALLGQGLRGVERETLRVDGQGHLARTPHPPALGSALTNDLIT 60 +TD TRRL L Q H LLGQGLRG+ERETLRV+ G LA TPHP ALGSALT+ IT Sbjct: 50 LTDHYTRRLALLGQEQHRGLLGQGLRGIERETLRVEDDGRLALTPHPRALGSALTHPQIT 109 Query: 61 TDYSESLLEFITPAMPDIADALQRLDEIHRFAYTKLDGEFLWNASMPCVLPPEDEIPLAW 120 TDYSESLLEFITPA DIA AL LD IHRF YTKL E LW+ SMP LP E++IP+AW Sbjct: 110 TDYSESLLEFITPAEHDIATALTELDAIHRFVYTKLGDELLWSQSMPAHLPAEEQIPIAW 169 Query: 121 YGSSHIGMLKHVYRRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLRDGDDTVDPSLSRRD 180 YG+SH+G LKHVYRRGLALRYG+ MQCIAGIHYN+SL+E +W+ L+ + ++ Sbjct: 170 YGTSHMGTLKHVYRRGLALRYGKRMQCIAGIHYNYSLAEGVWQALQQAEGASGTAVH--- 226 Query: 181 YQSERYIALIRNFRRYSWLLMYLFGASPAVATNFLRGRPHQLETLSDDTLFLPYGTSLRM 240 +QSE YIAL+RNFRRYSWLLMYLFGASPA++ FL GR H+LE L DTL+LPY TSLRM Sbjct: 227 FQSESYIALVRNFRRYSWLLMYLFGASPALSKCFLDGRDHRLEELDKDTLYLPYATSLRM 286 Query: 241 SDLGYQNHAQANITPHYNNLPAYIRSLAHAVSEPYPPYEAIGTKRDGEWVQINTNILQIE 300 SDLGY + AQA +TP +N+L +YI+SLA AVS+PYPPY IGT R+GEWVQINTNILQIE Sbjct: 287 SDLGYTSEAQARLTPQFNSLDSYIKSLARAVSQPYPPYAEIGTHRNGEWVQINTNILQIE 346 Query: 301 NEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEAARFLDVFLHF 360 NE+YS+IRPKRVINSGERP+QALSARGVQY+EVRCMDIDPF P+GI LE +RFLD FL F Sbjct: 347 NEYYSTIRPKRVINSGERPIQALSARGVQYIEVRCMDIDPFEPMGINLETSRFLDAFLLF 406 Query: 361 LALESSCLTSDEEGVENRANFLATVKEGRRPGLKLQFKGESLLLTDWGQQLLDRMEPVAD 420 AL+ S LTSDEE +EN NF VKEGRRPGL+L+ G + L WG++L+ R+ A Sbjct: 407 CALDDSPLTSDEESLENTENFSRAVKEGRRPGLQLRRDGNPVGLQTWGKELMQRIGSAAA 466 Query: 421 LLDRHLGGTQHRDSLAVQRAKLADPDSTPSARVLREIREAGNSFPAYTLANSQRQAAELI 480 LLD H SLA Q KL + TPSA+VL +R+ SF ++ L S+ AA + Sbjct: 467 LLDAQRSDGAHAASLAKQNLKLESVELTPSAKVLAILRDNQESFASFALRQSKAHAAYFL 526 Query: 481 ARPLSSSKTADFVAIAQASLDAQRDIERTQVGDFDTFVAAYRASTLGNIAI 531 A P S + F +A SL Q +ER+Q GDFDTFVAAYRASTLGN+++ Sbjct: 527 AHPPSPEQRNYFETLAATSLSEQESLERSQTGDFDTFVAAYRASTLGNMSV 577 >ref|YP_001352856.1| glutamate--cysteine ligase [Janthinobacterium sp. Marseille] sp|A6SX59|GSH1_JANMA RecName: Full=Glutamate--cysteine ligase; AltName: Full=Gamma-ECS; Short=GCS; AltName: Full=Gamma-glutamylcysteine synthetase gb|ABR88957.1| glutamate--cysteine ligase [Janthinobacterium sp. Marseille] Length = 528 Score = 656 bits (1693), Expect = 0.0, Method: Composition-based stats. Identities = 330/525 (62%), Positives = 397/525 (75%), Gaps = 3/525 (0%) Query: 1 MTDLLTRRLQQLSQPHHLALLGQGLRGVERETLRVDGQGHLARTPHPPALGSALTNDLIT 60 M++L TRRL L+ H LL QG RG+ERETLRVD QG LA PHP ALGSALTN IT Sbjct: 1 MSNLFTRRLALLADDAHRDLLNQGRRGIERETLRVDLQGKLALGPHPVALGSALTNPQIT 60 Query: 61 TDYSESLLEFITPAMPDIADALQRLDEIHRFAYTKLDGEFLWNASMPCVLPPEDEIPLAW 120 TDYSESLLEFITPA D+A AL+ LD IHRFA KLD E LW+ SMPC LP E +IP+AW Sbjct: 61 TDYSESLLEFITPAEHDVATALEELDRIHRFANAKLDHELLWSQSMPCTLPEEADIPIAW 120 Query: 121 YGSSHIGMLKHVYRRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLRDGDDTVDPSLSRRD 180 YG+SHIGM+KHVYRRGLALRYG+ MQCIAG+HYN+SLSEDLW V++ + + L + Sbjct: 121 YGTSHIGMIKHVYRRGLALRYGKAMQCIAGLHYNYSLSEDLWRVIKQSEQS---RLDDKS 177 Query: 181 YQSERYIALIRNFRRYSWLLMYLFGASPAVATNFLRGRPHQLETLSDDTLFLPYGTSLRM 240 YQSE YI+LIRNF+RYSWLLMYLFGASPA++T+FLRGR H+L+TLSDDTL+LPY TSLRM Sbjct: 178 YQSESYISLIRNFQRYSWLLMYLFGASPALSTHFLRGREHELQTLSDDTLYLPYATSLRM 237 Query: 241 SDLGYQNHAQANITPHYNNLPAYIRSLAHAVSEPYPPYEAIGTKRDGEWVQINTNILQIE 300 SDLGYQN+AQA + P YN+L +Y+RSL+ AV + YP YEAIGT+R+GEW+Q+NTN+LQIE Sbjct: 238 SDLGYQNNAQAGLMPPYNDLESYMRSLSRAVRQAYPAYEAIGTRRNGEWIQLNTNLLQIE 297 Query: 301 NEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEAARFLDVFLHF 360 NE+Y++IRPKRVINSGERPV+AL ARGVQY+EVRCMDIDPF PLGI L +RFLD FL F Sbjct: 298 NEYYATIRPKRVINSGERPVEALCARGVQYIEVRCMDIDPFEPLGISLPTSRFLDAFLLF 357 Query: 361 LALESSCLTSDEEGVENRANFLATVKEGRRPGLKLQFKGESLLLTDWGQQLLDRMEPVAD 420 AL+ S LT + E NF TVKEGRRPGL LQ G ++ L DWG +LL+R++ AD Sbjct: 358 CALDDSPLTDEANNRERTENFARTVKEGRRPGLLLQRDGAAVKLQDWGLELLERIQATAD 417 Query: 421 LLDRHLGGTQHRDSLAVQRAKLADPDSTPSARVLREIREAGNSFPAYTLANSQRQAAELI 480 LLD +QH +LA Q+ KL D TPSARVL E++ SF + L S A Sbjct: 418 LLDAQRADSQHAQALAAQKEKLLDAGLTPSARVLAELQATDKSFEQFGLRQSIAHAEYFR 477 Query: 481 ARPLSSSKTADFVAIAQASLDAQRDIERTQVGDFDTFVAAYRAST 525 ARPL++ + F +A+ S+ Q ++ERTQ GDFD FV AY T Sbjct: 478 ARPLNAEENFYFETLAKTSIAEQEEMERTQSGDFDAFVEAYNQRT 522 >ref|YP_002797731.1| glutamate--cysteine ligase [Azotobacter vinelandii DJ] sp|C1DJH1|GSH1_AZOVD RecName: Full=Glutamate--cysteine ligase; AltName: Full=Gamma-ECS; Short=GCS; AltName: Full=Gamma-glutamylcysteine synthetase gb|ACO76756.1| glutamate--cysteine ligase [Azotobacter vinelandii DJ] Length = 530 Score = 640 bits (1652), Expect = 0.0, Method: Composition-based stats. Identities = 326/528 (61%), Positives = 392/528 (74%) Query: 1 MTDLLTRRLQQLSQPHHLALLGQGLRGVERETLRVDGQGHLARTPHPPALGSALTNDLIT 60 M++L +RRL L +P + +LL L G+ERE LRVD G LARTPHP ALGSALT+ IT Sbjct: 1 MSELFSRRLALLGEPANQSLLRHCLHGIERECLRVDAAGELARTPHPAALGSALTHPYIT 60 Query: 61 TDYSESLLEFITPAMPDIADALQRLDEIHRFAYTKLDGEFLWNASMPCVLPPEDEIPLAW 120 TDYSE+LLEFITPA D A L L+ +HRFA KLDGE+LW+ SMPC LP E++IP+A Sbjct: 61 TDYSEALLEFITPAETDSAATLAALERVHRFACAKLDGEYLWSPSMPCPLPAEEDIPIAR 120 Query: 121 YGSSHIGMLKHVYRRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLRDGDDTVDPSLSRRD 180 YG+SHIG LKHVYR+GLALRYG+TMQCIAGIHYNFSL E+LW +L+ + V RD Sbjct: 121 YGNSHIGRLKHVYRKGLALRYGKTMQCIAGIHYNFSLPEELWPLLQRAEGDVRGVRGVRD 180 Query: 181 YQSERYIALIRNFRRYSWLLMYLFGASPAVATNFLRGRPHQLETLSDDTLFLPYGTSLRM 240 +QSERYIALIRNFRRYSWLLMYLFGASPA+ +FLRGRPH L+ L +TL+LP+ TSLRM Sbjct: 181 FQSERYIALIRNFRRYSWLLMYLFGASPALDKSFLRGRPHHLQELDAETLYLPWATSLRM 240 Query: 241 SDLGYQNHAQANITPHYNNLPAYIRSLAHAVSEPYPPYEAIGTKRDGEWVQINTNILQIE 300 SDLGY N+AQA++TP YN+L +Y SL AVS PY PY AIGT+RDGEW+Q+NTN+LQIE Sbjct: 241 SDLGYHNNAQADLTPCYNDLASYTDSLRRAVSTPYAPYAAIGTQRDGEWLQLNTNVLQIE 300 Query: 301 NEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEAARFLDVFLHF 360 NE+YS+IRPKRV SGERP+QAL ARGVQYVEVRC+DIDPF PLGI L+ ARFLD FL F Sbjct: 301 NEYYSTIRPKRVTASGERPIQALVARGVQYVEVRCLDIDPFLPLGIDLDEARFLDAFLLF 360 Query: 361 LALESSCLTSDEEGVENRANFLATVKEGRRPGLKLQFKGESLLLTDWGQQLLDRMEPVAD 420 ALE S + E NFL VKEGRRPGL LQ G+ + L W +LL+R++P+A Sbjct: 361 CALEDSPCLAAGECASCTGNFLKVVKEGRRPGLHLQRHGQPMPLAAWAGELLERLQPLAA 420 Query: 421 LLDRHLGGTQHRDSLAVQRAKLADPDSTPSARVLREIREAGNSFPAYTLANSQRQAAELI 480 L DR GG + +L Q+AK+ADP+ TPSAR+L +RE G SF A+ L S A L Sbjct: 421 LFDRAYGGDGYEKALHAQQAKVADPELTPSARLLATLRERGESFRAFALRQSLAHAESLR 480 Query: 481 ARPLSSSKTADFVAIAQASLDAQRDIERTQVGDFDTFVAAYRASTLGN 528 ARPL + F A A+ASL Q ++ER G FD FVAAY+AS G+ Sbjct: 481 ARPLGEEERQAFEAEARASLAEQAELERRDTGSFDDFVAAYQASIQGD 528 >ref|YP_793675.1| glutamate--cysteine ligase [Pseudomonas aeruginosa UCBPP-PA14] sp|Q02EG1|GSH1_PSEAB RecName: Full=Glutamate--cysteine ligase; AltName: Full=Gamma-ECS; Short=GCS; AltName: Full=Gamma-glutamylcysteine synthetase gb|ABJ14587.1| glutamate--cysteine ligase [Pseudomonas aeruginosa UCBPP-PA14] Length = 527 Score = 621 bits (1602), Expect = e-176, Method: Composition-based stats. Identities = 336/530 (63%), Positives = 398/530 (75%), Gaps = 4/530 (0%) Query: 1 MTDLLTRRLQQLSQPHHLALLGQGLRGVERETLRVDGQGHLARTPHPPALGSALTNDLIT 60 M+DLL+RRL L +L LL + L G+ERE LRVD G LA TPHP ALGS LT+ IT Sbjct: 1 MSDLLSRRLALLGAAANLPLLTECLHGIERECLRVDSDGKLALTPHPRALGSTLTHPQIT 60 Query: 61 TDYSESLLEFITPAMPDIADALQRLDEIHRFAYTKLDGEFLWNASMPCVLPPEDEIPLAW 120 TDYSE+LLEFITP D+AD L L+ IHRFA +KLDGE+LW+ SMPC LP E+ IP+A Sbjct: 61 TDYSEALLEFITPTETDVADTLGDLERIHRFASSKLDGEYLWSPSMPCELPDEESIPIAR 120 Query: 121 YGSSHIGMLKHVYRRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLRDGDDTVDPSLSRRD 180 YGSS IG LK+VYR+GLALRYG+TMQCIAGIHYNFSL E LW +LR + + LS RD Sbjct: 121 YGSSLIGRLKYVYRKGLALRYGKTMQCIAGIHYNFSLPERLWPLLRQAEGS---ELSERD 177 Query: 181 YQSERYIALIRNFRRYSWLLMYLFGASPAVATNFLRGRPHQLETLSDDTLFLPYGTSLRM 240 YQS YIALIRNFRRYSWLLMYLFGASPA+ FLRGRP QLE L + TL+LPY TSLRM Sbjct: 178 YQSAAYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRPSQLERLDEHTLYLPYATSLRM 237 Query: 241 SDLGYQNHAQANITPHYNNLPAYIRSLAHAVSEPYPPYEAIGTKRDGEWVQINTNILQIE 300 SDLGYQN+AQA +TP YN+L +YI SL AVS PYPPYE +GTK+DGEWVQ+NTNILQIE Sbjct: 238 SDLGYQNNAQAGLTPCYNDLQSYIDSLRQAVSTPYPPYEKVGTKQDGEWVQLNTNILQIE 297 Query: 301 NEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEAARFLDVFLHF 360 NE+YSSIRPKRV +GERPVQAL+ARGVQYVEVRC+DI+PF PLGI L+ ARFLD FL F Sbjct: 298 NEYYSSIRPKRVTYTGERPVQALAARGVQYVEVRCLDINPFLPLGIDLDEARFLDAFLLF 357 Query: 361 LALESSCLTSDEEGVENRANFLATVKEGRRPGLKLQFKGESLLLTDWGQQLLDRMEPVAD 420 A S L + E + NFLA VKEGRRPGL+LQ +G+ + L W +LL+R+ A Sbjct: 358 CAFSDSPLLNGECS-DATDNFLAVVKEGRRPGLQLQRRGQPVELKVWANELLERIADTAA 416 Query: 421 LLDRHLGGTQHRDSLAVQRAKLADPDSTPSARVLREIREAGNSFPAYTLANSQRQAAELI 480 LLDR GG H +LA QRAK+AD + TPSA+VL+ +RE G SF A++L S+ A Sbjct: 417 LLDRARGGEAHAAALAAQRAKVADAELTPSAQVLKVMRERGESFEAFSLRQSREHAEYFR 476 Query: 481 ARPLSSSKTADFVAIAQASLDAQRDIERTQVGDFDTFVAAYRASTLGNIA 530 PL++ + A F +A SL Q ++ER Q GDFDTFVAAY+AS LG I+ Sbjct: 477 QHPLAAEEQARFEKMASDSLAEQTELERDQDGDFDTFVAAYQASILGLIS 526