BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.3096.p (356 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774868.1| L-asparaginase/archaeal Glu-tRNAGln amidotra... 643 0.0 ref|YP_296021.1| L-asparaginase, type II [Ralstonia eutropha JMP... 483 e-134 ref|YP_583735.1| periplasmic L-asparaginase II [Cupriavidus meta... 475 e-132 ref|YP_004685710.1| glutaminase-asparaginase AnsB [Cupriavidus n... 472 e-131 ref|YP_726379.1| glutaminase-asparaginase (amidohydrolase) [Rals... 471 e-131 >ref|YP_003774868.1| L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D protein [Herbaspirillum seropedicae SmR1] emb|CAM32630.1| L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D protein [Herbaspirillum seropedicae] gb|ADJ62960.1| L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D protein [Herbaspirillum seropedicae SmR1] Length = 356 Score = 643 bits (1659), Expect = 0.0, Method: Composition-based stats. Identities = 348/356 (97%), Positives = 354/356 (99%) Query: 1 MQQAHFKKIGSVMILSLACAFAAHAQDKKPNVVVIGTGGTIAGAGASSVNTAAYQSAVVP 60 MQQA FKKIG+VMILSLACAF+A AQDKKPNVVVIGTGGTIAGAGASSVNTAAYQSAVVP Sbjct: 1 MQQAQFKKIGAVMILSLACAFSAQAQDKKPNVVVIGTGGTIAGAGASSVNTAAYQSAVVP 60 Query: 61 VDKIIAAVPEISKIANVRGEQIFQIGSESFNDERLIKLGKRVSELLKQSDVDGIVITHGT 120 VDKIIAAVPEISKIANVRGEQIFQIGSESFNDERLIKLGKRVSELLKQ+DVDGIVITHGT Sbjct: 61 VDKIIAAVPEISKIANVRGEQIFQIGSESFNDERLIKLGKRVSELLKQNDVDGIVITHGT 120 Query: 121 DTIEETAYFLNLVLKSEKPVVVVGSMRPGTALGADGALNLYDAVLVAGSKEAAGKGTLVV 180 DTIEETAYFLNLVLKS+KPVVVVGSMRPGTALGADGALNLYDAVLVAGSKEAAGKGTLVV Sbjct: 121 DTIEETAYFLNLVLKSDKPVVVVGSMRPGTALGADGALNLYDAVLVAGSKEAAGKGTLVV 180 Query: 181 MNDEIHSGRDVTKSNTFKVETFRSPYGPLGYVVENKLSFYRLPARPHTTQTEFDIDKISS 240 MNDEIHSGRDVTKSNTFKVETFRSPYGPLGYVVENKLSFYRLPARPHTTQTEFDIDKISS Sbjct: 181 MNDEIHSGRDVTKSNTFKVETFRSPYGPLGYVVENKLSFYRLPARPHTTQTEFDIDKISS 240 Query: 241 LPRVDIVYNYGNVSRTAYDAFVAAGAKAIIHDGTGNGSVAEQIVPVLQEVRSKGVQVIRA 300 LPRVDIVYNYGNVSRTAYDAFVAAGAKAIIHDGTGNGSVAEQIVPVLQEVRSKGVQVIRA Sbjct: 241 LPRVDIVYNYGNVSRTAYDAFVAAGAKAIIHDGTGNGSVAEQIVPVLQEVRSKGVQVIRA 300 Query: 301 SRTGSGVVIRNGEQPDDKYDWVVTDDQNAQKARILAELALTRTNDPKELQKIMWKY 356 SRTGSGVVIRNGEQPDDK+DWVVTDDQNAQKARILAELALT+TNDPKELQKIMWKY Sbjct: 301 SRTGSGVVIRNGEQPDDKFDWVVTDDQNAQKARILAELALTKTNDPKELQKIMWKY 356 >ref|YP_296021.1| L-asparaginase, type II [Ralstonia eutropha JMP134] gb|AAZ61177.1| L-asparaginase, type II [Ralstonia eutropha JMP134] Length = 374 Score = 483 bits (1243), Expect = e-134, Method: Composition-based stats. Identities = 261/335 (77%), Positives = 293/335 (87%) Query: 22 AAHAQDKKPNVVVIGTGGTIAGAGASSVNTAAYQSAVVPVDKIIAAVPEISKIANVRGEQ 81 A+ +K N+V+IGTGGTIAGAGAS+ NTAAYQSAVVPVDKIIA+VPEISK+ANV+GEQ Sbjct: 40 GTQAEARKANIVIIGTGGTIAGAGASATNTAAYQSAVVPVDKIIASVPEISKVANVKGEQ 99 Query: 82 IFQIGSESFNDERLIKLGKRVSELLKQSDVDGIVITHGTDTIEETAYFLNLVLKSEKPVV 141 IFQIGSESFN+ERL+KLGKRVSELLKQ DVDGIVITHGTDTIEETAYFLNL LKS+KPVV Sbjct: 100 IFQIGSESFNNERLLKLGKRVSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVV 159 Query: 142 VVGSMRPGTALGADGALNLYDAVLVAGSKEAAGKGTLVVMNDEIHSGRDVTKSNTFKVET 201 VVGSMRPGTALGADGALNLYDAVLVA + + GKGTL V+NDEIH+GRDVTKSNTFK ET Sbjct: 160 VVGSMRPGTALGADGALNLYDAVLVASNPASRGKGTLAVLNDEIHTGRDVTKSNTFKTET 219 Query: 202 FRSPYGPLGYVVENKLSFYRLPARPHTTQTEFDIDKISSLPRVDIVYNYGNVSRTAYDAF 261 FRSP+GPLGYVVE + FYRLPARPHTTQTEFDIDKI LP V +VY YGNV+ + DA Sbjct: 220 FRSPFGPLGYVVEGRTLFYRLPARPHTTQTEFDIDKIDKLPEVAVVYAYGNVNPGSVDAA 279 Query: 262 VAAGAKAIIHDGTGNGSVAEQIVPVLQEVRSKGVQVIRASRTGSGVVIRNGEQPDDKYDW 321 V GAKAII+ TGNGSV + +V L+ R+KGVQ++RASRTGSGVVIRN EQPDDKYDW Sbjct: 280 VKNGAKAIIYAATGNGSVGDYMVEPLKAARAKGVQIVRASRTGSGVVIRNAEQPDDKYDW 339 Query: 322 VVTDDQNAQKARILAELALTRTNDPKELQKIMWKY 356 +VTDDQ QKARIL LALT+TND K+LQ++MWKY Sbjct: 340 IVTDDQLPQKARILMALALTKTNDSKQLQQVMWKY 374 >ref|YP_583735.1| periplasmic L-asparaginase II [Cupriavidus metallidurans CH34] gb|ABF08466.1| periplasmic L-asparaginase II [Cupriavidus metallidurans CH34] Length = 375 Score = 475 bits (1222), Expect = e-132, Method: Composition-based stats. Identities = 261/332 (78%), Positives = 294/332 (88%) Query: 25 AQDKKPNVVVIGTGGTIAGAGASSVNTAAYQSAVVPVDKIIAAVPEISKIANVRGEQIFQ 84 A+ +K N+V+IGTGGTIAGAGA++ NTAAYQSAVVPVDKIIA+VPEISKIANV+GEQ+FQ Sbjct: 44 AEARKANIVIIGTGGTIAGAGAAATNTAAYQSAVVPVDKIIASVPEISKIANVKGEQVFQ 103 Query: 85 IGSESFNDERLIKLGKRVSELLKQSDVDGIVITHGTDTIEETAYFLNLVLKSEKPVVVVG 144 IGSESFN+ERLIKL KRVSELLKQ DVDGIVITHGTDTIEETAYFLNL LKS+KPVVVVG Sbjct: 104 IGSESFNNERLIKLAKRVSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVVVVG 163 Query: 145 SMRPGTALGADGALNLYDAVLVAGSKEAAGKGTLVVMNDEIHSGRDVTKSNTFKVETFRS 204 SMRPGTALGADGALNLYDAVLVA + + GKGTLVV+NDEI +GRDVTK+NTFKVETFRS Sbjct: 164 SMRPGTALGADGALNLYDAVLVASNPASKGKGTLVVLNDEIQTGRDVTKTNTFKVETFRS 223 Query: 205 PYGPLGYVVENKLSFYRLPARPHTTQTEFDIDKISSLPRVDIVYNYGNVSRTAYDAFVAA 264 P+GPLGYVVE + ++RLPARPHTTQTEFDIDKI LP V +VY YGN++ A DA V + Sbjct: 224 PFGPLGYVVEGRTLYFRLPARPHTTQTEFDIDKIDKLPEVAVVYAYGNMNPAAIDAAVKS 283 Query: 265 GAKAIIHDGTGNGSVAEQIVPVLQEVRSKGVQVIRASRTGSGVVIRNGEQPDDKYDWVVT 324 GAKAII D TGNGSV + +V L+ R+KGV ++RASRTGSGVVIRNGEQPDDKYDW+VT Sbjct: 284 GAKAIIFDATGNGSVGDYMVEPLKAARAKGVFIVRASRTGSGVVIRNGEQPDDKYDWIVT 343 Query: 325 DDQNAQKARILAELALTRTNDPKELQKIMWKY 356 DDQ AQKARIL LALT+TNDPK LQ+I WKY Sbjct: 344 DDQIAQKARILMALALTKTNDPKALQEIFWKY 375 >ref|YP_004685710.1| glutaminase-asparaginase AnsB [Cupriavidus necator N-1] gb|AEI77229.1| glutaminase-asparaginase AnsB [Cupriavidus necator N-1] Length = 374 Score = 472 bits (1214), Expect = e-131, Method: Composition-based stats. Identities = 255/334 (76%), Positives = 290/334 (86%) Query: 23 AHAQDKKPNVVVIGTGGTIAGAGASSVNTAAYQSAVVPVDKIIAAVPEISKIANVRGEQI 82 A A+ +K N+V+IGTGGTIAGAGAS+ NTAAYQSAVVPVDKIIA+VPEISK+ANV+GEQI Sbjct: 41 AQAEARKANIVIIGTGGTIAGAGASATNTAAYQSAVVPVDKIIASVPEISKVANVKGEQI 100 Query: 83 FQIGSESFNDERLIKLGKRVSELLKQSDVDGIVITHGTDTIEETAYFLNLVLKSEKPVVV 142 FQIGSESFN+ERL+KL KRVSELLKQ DVDGIVITHGTDTIEETAYFLNL LKS+KPVVV Sbjct: 101 FQIGSESFNNERLLKLAKRVSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVVV 160 Query: 143 VGSMRPGTALGADGALNLYDAVLVAGSKEAAGKGTLVVMNDEIHSGRDVTKSNTFKVETF 202 VGSMRPGTALGADGALNLYDAVLVA + + GKGTL V+NDEIH+GRDVTKSNTFK ETF Sbjct: 161 VGSMRPGTALGADGALNLYDAVLVASNPASKGKGTLAVLNDEIHTGRDVTKSNTFKTETF 220 Query: 203 RSPYGPLGYVVENKLSFYRLPARPHTTQTEFDIDKISSLPRVDIVYNYGNVSRTAYDAFV 262 RSP+GPLGYVVE + FYRLPARPHT QT++DIDKI LP V ++Y YGN + A DA V Sbjct: 221 RSPFGPLGYVVEGRTLFYRLPARPHTQQTQWDIDKIDKLPEVAVLYAYGNANPAAVDAAV 280 Query: 263 AAGAKAIIHDGTGNGSVAEQIVPVLQEVRSKGVQVIRASRTGSGVVIRNGEQPDDKYDWV 322 +GAKAII+ TGNGSV + +V L+ R+KGVQ++RASRTGSGVV+RN EQPDDKYDW+ Sbjct: 281 KSGAKAIIYAATGNGSVGDYMVESLKAARAKGVQIVRASRTGSGVVVRNAEQPDDKYDWI 340 Query: 323 VTDDQNAQKARILAELALTRTNDPKELQKIMWKY 356 VTDDQ QKARIL LALT+TND K LQ++ WKY Sbjct: 341 VTDDQLPQKARILMALALTQTNDSKALQQVFWKY 374 >ref|YP_726379.1| glutaminase-asparaginase (amidohydrolase) [Ralstonia eutropha H16] emb|CAJ93011.1| Glutaminase-asparaginase (amidohydrolase) [Ralstonia eutropha H16] Length = 374 Score = 471 bits (1212), Expect = e-131, Method: Composition-based stats. Identities = 253/329 (76%), Positives = 286/329 (86%) Query: 28 KKPNVVVIGTGGTIAGAGASSVNTAAYQSAVVPVDKIIAAVPEISKIANVRGEQIFQIGS 87 +K N+V+IGTGGTIAGAGAS NTAAYQSAVVPVDKIIA+VPEISK+ANV+GEQIFQIGS Sbjct: 46 RKANIVIIGTGGTIAGAGASVTNTAAYQSAVVPVDKIIASVPEISKVANVKGEQIFQIGS 105 Query: 88 ESFNDERLIKLGKRVSELLKQSDVDGIVITHGTDTIEETAYFLNLVLKSEKPVVVVGSMR 147 ESFN+ERL+KL KRVSELLKQ DVDGIVITHGTDTIEETAYFLNL LKS+KPVVVVGSMR Sbjct: 106 ESFNNERLLKLAKRVSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVVVVGSMR 165 Query: 148 PGTALGADGALNLYDAVLVAGSKEAAGKGTLVVMNDEIHSGRDVTKSNTFKVETFRSPYG 207 PGTALGADGALNLYDAVLVA + + GKGTL V+NDEIH+GRDVTKSNTFK ETFRSP+G Sbjct: 166 PGTALGADGALNLYDAVLVASNPASKGKGTLAVLNDEIHTGRDVTKSNTFKTETFRSPFG 225 Query: 208 PLGYVVENKLSFYRLPARPHTTQTEFDIDKISSLPRVDIVYNYGNVSRTAYDAFVAAGAK 267 PLGYVVE + FYRLPARPHT QT++DIDKI LP V ++Y YGN + A DA V +GAK Sbjct: 226 PLGYVVEGRTLFYRLPARPHTLQTQWDIDKIDKLPEVAVLYAYGNANPAAVDAAVKSGAK 285 Query: 268 AIIHDGTGNGSVAEQIVPVLQEVRSKGVQVIRASRTGSGVVIRNGEQPDDKYDWVVTDDQ 327 AII+ TGNGSV + +V L+ R+KGVQ++RASRTGSGVV+RN EQPDDKYDW+VTDDQ Sbjct: 286 AIIYAATGNGSVGDYMVESLKAARAKGVQIVRASRTGSGVVVRNAEQPDDKYDWIVTDDQ 345 Query: 328 NAQKARILAELALTRTNDPKELQKIMWKY 356 QKARIL LALT+TND K LQ++ WKY Sbjct: 346 LPQKARILMALALTQTNDAKALQQVFWKY 374