BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.3074.p (368 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774121.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m... 756 0.0 ref|YP_004754284.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m... 654 0.0 ref|YP_001098679.1| tRNA-specific 2-thiouridylase MnmA [Herminii... 640 0.0 ref|YP_001352063.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m... 635 e-180 ref|ZP_08275115.1| tRNA (5-methylaminomethyl-2-thiouridylate)-me... 620 e-175 >ref|YP_003774121.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Herbaspirillum seropedicae SmR1] gb|ADJ62213.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase protein [Herbaspirillum seropedicae SmR1] Length = 368 Score = 756 bits (1953), Expect = 0.0, Method: Composition-based stats. Identities = 361/368 (98%), Positives = 367/368 (99%) Query: 1 MSKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVA 60 MSKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVA Sbjct: 1 MSKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVA 60 Query: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADL 120 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADL Sbjct: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADL 120 Query: 121 IATGHYARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRKIA 180 IATGHYARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVR+IA Sbjct: 121 IATGHYARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRRIA 180 Query: 181 EQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQ 240 EQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQ Sbjct: 181 EQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQ 240 Query: 241 RKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQISWVAG 300 RKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQ+SWVAG Sbjct: 241 RKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQVSWVAG 300 Query: 301 HAPQLPALSAKTRYRQADVPCGFADAGSSFSLAFDTPQWAVTPGQSAVLYDGDVCLGGGI 360 HAPQ+ ALSAKTRYRQADVPCGF+DAG+SFSLAFDTPQWAVTPGQSAVLYDGDVCLGGGI Sbjct: 301 HAPQMAALSAKTRYRQADVPCGFSDAGASFSLAFDTPQWAVTPGQSAVLYDGDVCLGGGI 360 Query: 361 IESGALPD 368 IE+GALPD Sbjct: 361 IENGALPD 368 >ref|YP_004754284.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Collimonas fungivorans Ter331] gb|AEK63461.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Collimonas fungivorans Ter331] Length = 373 Score = 654 bits (1687), Expect = 0.0, Method: Composition-based stats. Identities = 313/366 (85%), Positives = 332/366 (90%), Gaps = 4/366 (1%) Query: 2 SKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVAD 61 SKKRVVIGMSGGVDSSVSAW+LK+QGYEVIGLFMKNWEDDDDSEYCSTR+DWIDAVSVAD Sbjct: 3 SKKRVVIGMSGGVDSSVSAWMLKQQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDAVSVAD 62 Query: 62 VVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADLI 121 V+GVDIEAVNFA+EYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADLI Sbjct: 63 VIGVDIEAVNFAAEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADLI 122 Query: 122 ATGHYARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRKIAE 181 ATGHYARVRQ G F+LLKA+DASKDQSYFLHRLNQ QLSRT+FPLGEI KTEVRKIAE Sbjct: 123 ATGHYARVRQADSGRFELLKAVDASKDQSYFLHRLNQSQLSRTLFPLGEIQKTEVRKIAE 182 Query: 182 QLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQR 241 Q+QLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGP+KT DG VGEHVGLSFYTLGQR Sbjct: 183 QIQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPMKTPDGDVVGEHVGLSFYTLGQR 242 Query: 242 KGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQISWVAGH 301 KGIG+GG+K H+NA G D WYVARKDVENNTLYIVQGHDHPWLLS TL AGQ+SWVAG Sbjct: 243 KGIGLGGMKTHKNAGGDSDPWYVARKDVENNTLYIVQGHDHPWLLSSTLQAGQLSWVAGE 302 Query: 302 APQLPALSAKTRYRQADVPCGFAD----AGSSFSLAFDTPQWAVTPGQSAVLYDGDVCLG 357 AP LSAKTRYRQAD+ C F +F+L+F PQWAVTPGQSAVLYDGD+CLG Sbjct: 303 APGSGQLSAKTRYRQADMACTFDSLRLPEAENFALSFSDPQWAVTPGQSAVLYDGDICLG 362 Query: 358 GGIIES 363 GGIIES Sbjct: 363 GGIIES 368 >ref|YP_001098679.1| tRNA-specific 2-thiouridylase MnmA [Herminiimonas arsenicoxydans] sp|A4G213|MNMA_HERAR RecName: Full=tRNA-specific 2-thiouridylase mnmA emb|CAL60550.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Herminiimonas arsenicoxydans] Length = 372 Score = 640 bits (1652), Expect = 0.0, Method: Composition-based stats. Identities = 308/372 (82%), Positives = 330/372 (88%), Gaps = 4/372 (1%) Query: 1 MSKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVA 60 M+KKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTR+DWIDA SVA Sbjct: 1 MAKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDAASVA 60 Query: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADL 120 DVVGVDIEAVNFA+EYKDRVFAEFLREY+AGRTPNPDVLCNAEIKFKAFLDHAM LGAD+ Sbjct: 61 DVVGVDIEAVNFAAEYKDRVFAEFLREYEAGRTPNPDVLCNAEIKFKAFLDHAMLLGADM 120 Query: 121 IATGHYARVRQ---GPQ-GDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEV 176 IATGHYARVR+ GP G QLLKA+DASKDQSYFLHRLNQ QLS+T+FPLGEI KTEV Sbjct: 121 IATGHYARVREVTSGPDAGQVQLLKAVDASKDQSYFLHRLNQAQLSKTLFPLGEIRKTEV 180 Query: 177 RKIAEQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFY 236 RKIAEQLQLPNA KKDSTGICFIGERPFREFLNRYLSY+PGP+KT DG VGEHVGLSFY Sbjct: 181 RKIAEQLQLPNASKKDSTGICFIGERPFREFLNRYLSYQPGPMKTPDGTIVGEHVGLSFY 240 Query: 237 TLGQRKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQIS 296 TLGQRKGIG+GG+K H+N DG+ WYVARKDV NNTLYIVQGHDHPWLLS LSAGQ+S Sbjct: 241 TLGQRKGIGLGGMKTHKNTDGNSGPWYVARKDVANNTLYIVQGHDHPWLLSSALSAGQMS 300 Query: 297 WVAGHAPQLPALSAKTRYRQADVPCGFADAGSSFSLAFDTPQWAVTPGQSAVLYDGDVCL 356 WVAG AP ++AKTRYRQADV C + +LAFDTPQWAVTPGQSAVLY GDVCL Sbjct: 301 WVAGRAPSEELVAAKTRYRQADVACSQHATADNLALAFDTPQWAVTPGQSAVLYQGDVCL 360 Query: 357 GGGIIESGALPD 368 GGGII + L + Sbjct: 361 GGGIITASTLTE 372 >ref|YP_001352063.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Janthinobacterium sp. Marseille] sp|A6SUW6|MNMA_JANMA RecName: Full=tRNA-specific 2-thiouridylase mnmA gb|ABR89509.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Janthinobacterium sp. Marseille] Length = 372 Score = 635 bits (1637), Expect = e-180, Method: Composition-based stats. Identities = 306/371 (82%), Positives = 329/371 (88%), Gaps = 4/371 (1%) Query: 1 MSKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVA 60 M+KKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTR+DWIDA SVA Sbjct: 1 MAKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDAASVA 60 Query: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADL 120 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAM LGAD+ Sbjct: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMLLGADM 120 Query: 121 IATGHYARVRQ---GPQ-GDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEV 176 IATGHYARVR+ GP G +LLKA+DASKDQSYFLHRLNQ QL++T+FPLGEI KTEV Sbjct: 121 IATGHYARVREVTSGPDAGRVELLKAVDASKDQSYFLHRLNQAQLAKTLFPLGEIRKTEV 180 Query: 177 RKIAEQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFY 236 RKIAEQL+LPNA KKDSTGICFIGERPFREFLNRYLSY+PGP+KT +G VGEHVGLSFY Sbjct: 181 RKIAEQLKLPNATKKDSTGICFIGERPFREFLNRYLSYQPGPMKTPEGVIVGEHVGLSFY 240 Query: 237 TLGQRKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQIS 296 TLGQRKGIG+GG+K+H+N DG+ + WYVARKDV NTLYIVQGHDHPWLLS LSAGQ+S Sbjct: 241 TLGQRKGIGLGGMKSHKNTDGNSEPWYVARKDVATNTLYIVQGHDHPWLLSSELSAGQMS 300 Query: 297 WVAGHAPQLPALSAKTRYRQADVPCGFADAGSSFSLAFDTPQWAVTPGQSAVLYDGDVCL 356 WVAG P +SAKTRYRQADV C FSLAF PQWAVTPGQSAVLY GDVCL Sbjct: 301 WVAGSPPSEELVSAKTRYRQADVACSQRSDADGFSLAFAAPQWAVTPGQSAVLYQGDVCL 360 Query: 357 GGGIIESGALP 367 GGGII + A+P Sbjct: 361 GGGIINTSAVP 371 >ref|ZP_08275115.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Oxalobacteraceae bacterium IMCC9480] gb|EGF31411.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Oxalobacteraceae bacterium IMCC9480] Length = 369 Score = 620 bits (1598), Expect = e-175, Method: Composition-based stats. Identities = 300/367 (81%), Positives = 327/367 (89%), Gaps = 5/367 (1%) Query: 1 MSKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVA 60 MSKK+VVIG+SGGVDSSVSA LLKEQGYEVIGLFMKNWEDDDD+EYCSTR+DWIDAVSVA Sbjct: 1 MSKKKVVIGLSGGVDSSVSAALLKEQGYEVIGLFMKNWEDDDDAEYCSTRQDWIDAVSVA 60 Query: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADL 120 DV+G+DIEAVNF++EYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAM LGADL Sbjct: 61 DVLGIDIEAVNFSAEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMHLGADL 120 Query: 121 IATGHYARVRQGPQ--GDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRK 178 IATGHYARVR+ G F+LLKA+D +KDQSYFLHRLNQ QLS+T+FPLGEI KTEVRK Sbjct: 121 IATGHYARVRESASDAGRFELLKAIDHTKDQSYFLHRLNQAQLSKTLFPLGEIRKTEVRK 180 Query: 179 IAEQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTL 238 +AEQL LPNA KKDSTGICFIGERPFREFLNRYLSY+PGP+KT +G T+GEHVGLSFYTL Sbjct: 181 MAEQLGLPNAAKKDSTGICFIGERPFREFLNRYLSYQPGPMKTPEGDTIGEHVGLSFYTL 240 Query: 239 GQRKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQISWV 298 GQRKGIGIGG+KAHQNA+G DAWYVARKD+ +NTLY+VQGHDHPWLLS +L A Q+SW+ Sbjct: 241 GQRKGIGIGGIKAHQNANGSSDAWYVARKDIASNTLYVVQGHDHPWLLSASLEADQLSWI 300 Query: 299 AGHAPQLPALSAKTRYRQADVPC--GFADAGSSFSLAFDTPQWAVTPGQSAVLYDGDVCL 356 AG P LSAKTRYRQADVPC G DA LAFD QWAVTPGQSAVLY GDVCL Sbjct: 301 AGAPPDDGQLSAKTRYRQADVPCHVGMVDA-QRLQLAFDASQWAVTPGQSAVLYRGDVCL 359 Query: 357 GGGIIES 363 GGGII S Sbjct: 360 GGGIIAS 366