BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2993.p (401 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seroped... 705 0.0 ref|YP_004752826.1| cysteine desulfurase [Collimonas fungivorans... 529 e-148 ref|ZP_03697045.1| cysteine desulfurase IscS [Lutiella nitroferr... 478 e-132 ref|YP_004684938.1| cysteine desulfurase IscS [Cupriavidus necat... 475 e-132 ref|YP_933520.1| cysteine desulfurase [Azoarcus sp. BH72] >gi|11... 475 e-132 >ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] gb|ADJ63074.1| cysteine desulfurase protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 705 bits (1819), Expect = 0.0, Method: Composition-based stats. Identities = 363/392 (92%), Positives = 374/392 (95%) Query: 1 MNAPAPCRPPADLRFPIYLDYGATTPVDPHVARVMCDFLTEKFGNAASSSHPFGWEARRA 60 MNAPAPCRPPADLRFPIYLDYGATTPVDP VARVMCDFLT+KFGNAASSSHPFGWEARRA Sbjct: 1 MNAPAPCRPPADLRFPIYLDYGATTPVDPQVARVMCDFLTDKFGNAASSSHPFGWEARRA 60 Query: 61 VDKARAQVAGLVGARPDEIIWTSGATESNNLALKGCAHALRAQGRGKHLITVATEHKAVL 120 V++ARAQVAGLVGA+P EI+WTSGATESNNLALKG A+ALRAQGRG HLITV TEHKAVL Sbjct: 61 VEQARAQVAGLVGAQPGEIVWTSGATESNNLALKGAAYALRAQGRGNHLITVPTEHKAVL 120 Query: 121 DTMHTLEKSGFEVTYLQPGADGLITPAQFRAALRPDTLLASVMYVNNEIGVIQPIAELGE 180 DTMHTLEK+GFEVTYLQPGADGLITP Q RAALRPDTLLASVMYVNNEIGVIQPIAELGE Sbjct: 121 DTMHTLEKAGFEVTYLQPGADGLITPQQLRAALRPDTLLASVMYVNNEIGVIQPIAELGE 180 Query: 181 LCAERGVVFHVDAVQAAGKIAIDLQQLKVDLMSFSAHKVYGPKGIGALYVRHNPALKLEA 240 +CA VVFHVDAVQAAGKIAIDL +LKVDLMSFSAHKVYGPKGIGALYVRHNP LKLEA Sbjct: 181 ICAAHDVVFHVDAVQAAGKIAIDLTRLKVDLMSFSAHKVYGPKGIGALYVRHNPQLKLEA 240 Query: 241 QIDGGGHENGMRSGTLATHQIVGMGEAFALAGDLLEEEGRRIRALRDRLWAGLADLPGVQ 300 QIDGGGHENGMRSGTLATHQIVGMGEAFALAG LLEEE RRI ALRDRLWAGLADLPGVQ Sbjct: 241 QIDGGGHENGMRSGTLATHQIVGMGEAFALAGRLLEEESRRIGALRDRLWAGLADLPGVQ 300 Query: 301 LNGSAEARVPHNLNVSFPPTRYGTLGSQLLGIAVSAGSACNSAASAPSFVLLAIGRSPDV 360 LNG A RVPHNLNVSFPPTR+GTLGSQLLGIAVSAGSACNSAA+APSFVLLAIGRSPDV Sbjct: 301 LNGCARQRVPHNLNVSFPPTRHGTLGSQLLGIAVSAGSACNSAAAAPSFVLLAIGRSPDV 360 Query: 361 ARNAVRFSLGRGTTEAEIDYVVATVRQVLAHE 392 ARNAVRFSLGRGTTEAEIDYVVATVRQVLAHE Sbjct: 361 ARNAVRFSLGRGTTEAEIDYVVATVRQVLAHE 392 >ref|YP_004752826.1| cysteine desulfurase [Collimonas fungivorans Ter331] gb|AEK62003.1| Cysteine desulfurase [Collimonas fungivorans Ter331] Length = 397 Score = 529 bits (1363), Expect = e-148, Method: Composition-based stats. Identities = 271/379 (71%), Positives = 316/379 (83%) Query: 11 ADLRFPIYLDYGATTPVDPHVARVMCDFLTEKFGNAASSSHPFGWEARRAVDKARAQVAG 70 A + PIYLDY ATTPVDP VA MC++LTEKFGN ASSSH FGWEA+ AV++AR QVA Sbjct: 17 ASPQLPIYLDYSATTPVDPAVAVKMCEYLTEKFGNPASSSHAFGWEAKAAVEQARRQVAA 76 Query: 71 LVGARPDEIIWTSGATESNNLALKGCAHALRAQGRGKHLITVATEHKAVLDTMHTLEKSG 130 LVGA+ EI+WTSGATESNNLALKG AH LR +GRG+HL+T++TEHKAVLDTM TLE+ G Sbjct: 77 LVGAQASEIVWTSGATESNNLALKGAAHVLRERGRGRHLVTMSTEHKAVLDTMRTLEREG 136 Query: 131 FEVTYLQPGADGLITPAQFRAALRPDTLLASVMYVNNEIGVIQPIAELGELCAERGVVFH 190 FEVTYL P +GL+ F+AALR DT++ASVM VNNEIGVIQP+AELGE+CA R +VFH Sbjct: 137 FEVTYLAPQPNGLVDLETFKAALRSDTIIASVMMVNNEIGVIQPVAELGEICAGRDIVFH 196 Query: 191 VDAVQAAGKIAIDLQQLKVDLMSFSAHKVYGPKGIGALYVRHNPALKLEAQIDGGGHENG 250 VDA QAAGK+AIDL+ LKV+LMSFSAHKVYGPKGIGAL+VRH+PALKLEAQIDGGGHENG Sbjct: 197 VDAAQAAGKVAIDLRSLKVNLMSFSAHKVYGPKGIGALFVRHDPALKLEAQIDGGGHENG 256 Query: 251 MRSGTLATHQIVGMGEAFALAGDLLEEEGRRIRALRDRLWAGLADLPGVQLNGSAEARVP 310 MRSGTLATHQIVGMGEAF+LA LL+ E RIR+L++RLW GL D PGV LNG RVP Sbjct: 257 MRSGTLATHQIVGMGEAFSLAAKLLDSENARIRSLQERLWDGLRDCPGVVLNGDRLQRVP 316 Query: 311 HNLNVSFPPTRYGTLGSQLLGIAVSAGSACNSAASAPSFVLLAIGRSPDVARNAVRFSLG 370 HNLN+SF + +QLL IAVSAGSACNSA +APS+VL ++GR +AR+A+RFSLG Sbjct: 317 HNLNLSFEIPGSTPIAAQLLDIAVSAGSACNSANAAPSYVLSSLGRPDALARSAIRFSLG 376 Query: 371 RGTTEAEIDYVVATVRQVL 389 R T+ EIDY VAT+RQ+L Sbjct: 377 RYTSAQEIDYTVATLRQLL 395 >ref|ZP_03697045.1| cysteine desulfurase IscS [Lutiella nitroferrum 2002] ref|YP_004848591.1| cysteine desulfurase IscS [Pseudogulbenkiania sp. NH8B] gb|EEG10565.1| cysteine desulfurase IscS [Lutiella nitroferrum 2002] dbj|BAK78177.1| cysteine desulfurase IscS [Pseudogulbenkiania sp. NH8B] Length = 405 Score = 478 bits (1229), Expect = e-132, Method: Composition-based stats. Identities = 235/377 (62%), Positives = 286/377 (75%), Gaps = 2/377 (0%) Query: 11 ADLRFPIYLDYGATTPVDPHVARVMCDFLTEKFGNAASSSHPFGWEARRAVDKARAQVAG 70 + L+ PIYLDY ATTPVDP VA M +LTEKFGN AS SH FGWEA AV++ARA VA Sbjct: 2 SQLKLPIYLDYSATTPVDPRVAEQMVVYLTEKFGNPASRSHSFGWEAEAAVEQARADVAA 61 Query: 71 LVGARPDEIIWTSGATESNNLALKGCAHALRAQGRGKHLITVATEHKAVLDTMHTLEKSG 130 LV P EIIWTSGATES+NLA+KG A ++ +GKH+ITV TEHKAVLDT LE+ G Sbjct: 62 LVNCDPKEIIWTSGATESDNLAIKGAAQFYKS--KGKHIITVKTEHKAVLDTCRELERQG 119 Query: 131 FEVTYLQPGADGLITPAQFRAALRPDTLLASVMYVNNEIGVIQPIAELGELCAERGVVFH 190 FEVTYL +GLI F+AALRPDT+L SVMYVNNE+GVIQ I ++GE+C E+G+VFH Sbjct: 120 FEVTYLGVKDNGLIDLDDFKAALRPDTILVSVMYVNNEVGVIQDIPQIGEICREKGIVFH 179 Query: 191 VDAVQAAGKIAIDLQQLKVDLMSFSAHKVYGPKGIGALYVRHNPALKLEAQIDGGGHENG 250 VDA QA GK+ IDL QLKVDLMSFSAHK YGPKGIGALYVR P ++LEAQ+ GGGHE G Sbjct: 180 VDAAQATGKVDIDLSQLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERG 239 Query: 251 MRSGTLATHQIVGMGEAFALAGDLLEEEGRRIRALRDRLWAGLADLPGVQLNGSAEARVP 310 RSGTLA HQIVGMG+AF +A + + E RIR LRDRLW G+ D+ V +NG +ARVP Sbjct: 240 FRSGTLAPHQIVGMGQAFRIAREEMATENERIRMLRDRLWNGIKDMEEVYINGDIDARVP 299 Query: 311 HNLNVSFPPTRYGTLGSQLLGIAVSAGSACNSAASAPSFVLLAIGRSPDVARNAVRFSLG 370 HNLNVSF +L + +AVS+GSAC SA+ PS+VL A+GR+ ++A +++RF++G Sbjct: 300 HNLNVSFNFVEGESLIMAVRDLAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTIG 359 Query: 371 RGTTEAEIDYVVATVRQ 387 R TTE EIDY + +R+ Sbjct: 360 RFTTEEEIDYTIKLMRE 376 >ref|YP_004684938.1| cysteine desulfurase IscS [Cupriavidus necator N-1] gb|AEI76457.1| cysteine desulfurase IscS [Cupriavidus necator N-1] Length = 405 Score = 475 bits (1223), Expect = e-132, Method: Composition-based stats. Identities = 232/372 (62%), Positives = 284/372 (76%), Gaps = 2/372 (0%) Query: 15 FPIYLDYGATTPVDPHVARVMCDFLTEKFGNAASSSHPFGWEARRAVDKARAQVAGLVGA 74 FPIY+DY ATTPVDP VA M +L E+FGN AS SH +GWEA RAV++AR QVA LVGA Sbjct: 6 FPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWEAERAVEEAREQVAALVGA 65 Query: 75 RPDEIIWTSGATESNNLALKGCAHALRAQGRGKHLITVATEHKAVLDTMHTLEKSGFEVT 134 P EI+WTSGATESNNLA+KG A+ G+GKHLIT+ TEHKAVLDT LE+ GFEVT Sbjct: 66 DPREIVWTSGATESNNLAIKGAANFY--SGKGKHLITLKTEHKAVLDTTRELERQGFEVT 123 Query: 135 YLQPGADGLITPAQFRAALRPDTLLASVMYVNNEIGVIQPIAELGELCAERGVVFHVDAV 194 YL +GL+ F+ ALRPDT+L SVM VNNEIGVIQ + ++GE+C E+G++FH DA Sbjct: 124 YLDVKENGLLDMEVFKQALRPDTILVSVMLVNNEIGVIQDVEQIGEICREKGIIFHCDAA 183 Query: 195 QAAGKIAIDLQQLKVDLMSFSAHKVYGPKGIGALYVRHNPALKLEAQIDGGGHENGMRSG 254 QA GK+AIDL +LK DLMSFSAHK YGPKGIGALYVR P +++EAQ+ GGGHE GMRSG Sbjct: 184 QATGKVAIDLNKLKCDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERGMRSG 243 Query: 255 TLATHQIVGMGEAFALAGDLLEEEGRRIRALRDRLWAGLADLPGVQLNGSAEARVPHNLN 314 TLATHQIVGMGEAF +A + + E RIR LRDRLW GL+D+ V LNG E RVPHNLN Sbjct: 244 TLATHQIVGMGEAFRIAREEMATENERIRMLRDRLWNGLSDMEEVYLNGDLEQRVPHNLN 303 Query: 315 VSFPPTRYGTLGSQLLGIAVSAGSACNSAASAPSFVLLAIGRSPDVARNAVRFSLGRGTT 374 VSF +L + +AVS+GSAC SA+ PS+VL A+GR+ ++A +++RF++GR TT Sbjct: 304 VSFNFVEGESLIMAIKDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTT 363 Query: 375 EAEIDYVVATVR 386 E EIDY + ++ Sbjct: 364 EQEIDYTIELLK 375 >ref|YP_933520.1| cysteine desulfurase [Azoarcus sp. BH72] emb|CAL94633.1| cysteine desulfurase [Azoarcus sp. BH72] Length = 403 Score = 475 bits (1222), Expect = e-132, Method: Composition-based stats. Identities = 237/370 (64%), Positives = 285/370 (77%), Gaps = 2/370 (0%) Query: 13 LRFPIYLDYGATTPVDPHVARVMCDFLTEKFGNAASSSHPFGWEARRAVDKARAQVAGLV 72 L+FPIYLDY ATTPVDP VA M +LTE FGN AS SH FGWEA RAV++AR +VA LV Sbjct: 2 LKFPIYLDYSATTPVDPRVAAKMIPWLTEHFGNPASRSHAFGWEAERAVEEARDEVAKLV 61 Query: 73 GARPDEIIWTSGATESNNLALKGCAHALRAQGRGKHLITVATEHKAVLDTMHTLEKSGFE 132 A P EI+WTSGATESNNLA+KG AH R+ +GKH+IT+ TEHKAVLDT+ LE+ GFE Sbjct: 62 NADPKEIVWTSGATESNNLAIKGAAHFYRS--KGKHVITLKTEHKAVLDTVRELEREGFE 119 Query: 133 VTYLQPGADGLITPAQFRAALRPDTLLASVMYVNNEIGVIQPIAELGELCAERGVVFHVD 192 TYL +GLI F+AALRPDT + SVM+VNNEIGVIQPIAE+GE+C E+G+VFHVD Sbjct: 120 ATYLDVQENGLIDLEAFKAALRPDTTVVSVMFVNNEIGVIQPIAEIGEICREKGIVFHVD 179 Query: 193 AVQAAGKIAIDLQQLKVDLMSFSAHKVYGPKGIGALYVRHNPALKLEAQIDGGGHENGMR 252 A QA GK+ IDL +LKVDLMSFSAHK YGPKGIGALYVR P ++LEAQ+ GGGHE G+R Sbjct: 180 AAQATGKVEIDLAKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERGLR 239 Query: 253 SGTLATHQIVGMGEAFALAGDLLEEEGRRIRALRDRLWAGLADLPGVQLNGSAEARVPHN 312 SGTLATHQIVGMGEAF +A + + EE RIR LRD+L GL D+ +NG E RVPHN Sbjct: 240 SGTLATHQIVGMGEAFRIAREEMAEENGRIRRLRDKLLNGLTDIDATYVNGDVEHRVPHN 299 Query: 313 LNVSFPPTRYGTLGSQLLGIAVSAGSACNSAASAPSFVLLAIGRSPDVARNAVRFSLGRG 372 LN+SF +L + IAVS+GSAC SA+ PS+VL A+GR ++A +++RF++GR Sbjct: 300 LNISFAYVEGESLIMAIKDIAVSSGSACTSASLEPSYVLRALGRDDELAHSSIRFTIGRF 359 Query: 373 TTEAEIDYVV 382 TTE EIDY + Sbjct: 360 TTEEEIDYAI 369