BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2954.p (591 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774959.1| outer membrane lipoprotein [Herbaspirillum s... 1019 0.0 ref|YP_004751831.1| outer membrane protein [Collimonas fungivora... 737 0.0 ref|YP_001900156.1| surface antigen (D15) [Ralstonia pickettii 1... 622 e-176 ref|NP_520505.1| lipoprotein [Ralstonia solanacearum GMI1000] >g... 620 e-175 emb|CBJ37198.1| Conserved protein of unknown function; similar t... 619 e-175 >ref|YP_003774959.1| outer membrane lipoprotein [Herbaspirillum seropedicae SmR1] gb|ADJ63051.1| outer membrane lipoprotein [Herbaspirillum seropedicae SmR1] Length = 588 Score = 1019 bits (2635), Expect = 0.0, Method: Composition-based stats. Identities = 509/591 (86%), Positives = 548/591 (92%), Gaps = 3/591 (0%) Query: 1 MTWTRSLAIAFLFCAADTAHAAYKVQIDAPKPVQSLLKDFLDISRYQDRDDISDEQLKFM 60 M WTRSLAI L+CAA+ A AAY+V I+APKPVQSLLKDFLD+SRYQDRDDISDEQLKFM Sbjct: 1 MKWTRSLAITLLWCAAEPAIAAYQVDIEAPKPVQSLLKDFLDLSRYQDRDDISDEQLKFM 60 Query: 61 MDTAGDQVRELTSTEGYFTPVTKVSAQTEAGAEGKQAVRRITISVDPGPRTEISSAEVSV 120 MDTAG+QVR+LT+TEGYFTPVTKVS ++ AG +G VRRIT++VDPGPRT + SAE+SV Sbjct: 61 MDTAGEQVRDLTATEGYFTPVTKVSEESAAGKDG---VRRITVTVDPGPRTLVESAEISV 117 Query: 121 TGAAARDDQATVARLREDWSLPVGQPFRQEDWNRAKNAGLEKLQQKKYAAARIASSEARV 180 TG+AA D A VA+LR DWSL G+PFRQ+DW++AKNAGL LQQKKYAAARIA SEAR+ Sbjct: 118 TGSAASQDAARVAQLRTDWSLSPGEPFRQQDWDKAKNAGLATLQQKKYAAARIADSEARI 177 Query: 181 DPDEHQADLAVRYDSGPAFTLGPLQITGTRRYPESIIRNVNPLTVGEPYDVNRLLALQRQ 240 P QADL+VRYDSGPAFTLG LQITGTRRYPESIIRNV+PL VGEPYDV+RLLALQRQ Sbjct: 178 APQAQQADLSVRYDSGPAFTLGALQITGTRRYPESIIRNVSPLIVGEPYDVDRLLALQRQ 237 Query: 241 IQNTPYFSNAIVAIDDDPEHPEQTPVKVQVTEFPTQRVRAGVGYSTDTGAQVEGRYSHYN 300 IQNTPYFSNAIVAIDDDP HP+QTPVKVQVTEFPTQR+R GVGY TDTGAQ EGRYSHYN Sbjct: 238 IQNTPYFSNAIVAIDDDPAHPDQTPVKVQVTEFPTQRIRTGVGYGTDTGAQAEGRYSHYN 297 Query: 301 VFNKAYVFDSQLRLEQKRQYGVLSLAMPPDDKAFVNSINTSYDRTKLEGIDLRSQQVGFK 360 VFN+AYVFDSQLRLEQKRQYG+LSLAMPPD KAFVNSINT+YDRT LEGIDLR+QQVG K Sbjct: 298 VFNRAYVFDSQLRLEQKRQYGLLSLAMPPDSKAFVNSINTAYDRTTLEGIDLRTQQVGLK 357 Query: 361 RARNSENYDTTYSLTYYRDELSQEDGSTLPANTIVTPGKHRALVPGFAWSRRNVDDPIFP 420 RARNS+NYDTTYSLTYYRDEL QE+G TLPANTIVTPGKHRALVPGFAWSRRNVD+PIFP Sbjct: 358 RARNSDNYDTTYSLTYYRDELVQENGVTLPANTIVTPGKHRALVPGFAWSRRNVDNPIFP 417 Query: 421 RKGNLLTLEAGFALKGLLTDQSFSRLYGRFKQFVPVGKRDIVVLRAELGGVFTAGSAGAV 480 R GNLLTLEAG ALKG+LTDQSFSRLYGRFKQFVPVGKRDIVVLRAELGGVFTAG AG+V Sbjct: 418 RHGNLLTLEAGLALKGVLTDQSFSRLYGRFKQFVPVGKRDIVVLRAELGGVFTAGRAGSV 477 Query: 481 PPSLLFRTGGNDSVRGYSYQSIGNEQNGTVYPTKYLAVGSSEYQRWFSENWGAAVFYDVG 540 P SLLFRTGGNDSVRGYSYQSIGNEQNGTVYPTKYLAVGS+EYQRWFSENWGAAVFYD+G Sbjct: 478 PASLLFRTGGNDSVRGYSYQSIGNEQNGTVYPTKYLAVGSTEYQRWFSENWGAAVFYDMG 537 Query: 541 AAADNWNNKTFYHGVGTGARWRSPVGTLNLDLAYGIQKRQIRPHISLGIAF 591 AAADNW+NKTFYHGVGTGARWRSPVGTLNLDLAYGIQKRQIRPHISLGIAF Sbjct: 538 AAADNWSNKTFYHGVGTGARWRSPVGTLNLDLAYGIQKRQIRPHISLGIAF 588 >ref|YP_004751831.1| outer membrane protein [Collimonas fungivorans Ter331] gb|AEK61008.1| Outer membrane protein [Collimonas fungivorans Ter331] Length = 596 Score = 737 bits (1903), Expect = 0.0, Method: Composition-based stats. Identities = 352/569 (61%), Positives = 451/569 (79%), Gaps = 7/569 (1%) Query: 23 YKVQIDAPKPVQSLLKDFLDISRYQDRDDISDEQLKFMMDTAGDQVRELTSTEGYFTPVT 82 Y+V+IDAP +++LL+ LD+SRY+DR D+S++QLKF++DT +QV +LTSTEGYF+P Sbjct: 35 YRVEIDAPGDLKALLEQHLDLSRYKDRQDLSEDQLKFLVDTVNEQVTQLTSTEGYFSP-- 92 Query: 83 KVSAQTEAGAEGKQAVRRITISVDPGPRTEISSAEVSVTGAAARDDQATVARLREDWSLP 142 K S E G V+ I + VDP RT +S+A V V+G+AA + V +++++W LP Sbjct: 93 KTSVTVEPGE-----VKTIHLKVDPQQRTIVSAATVDVSGSAAAEVPERVRQIQQNWGLP 147 Query: 143 VGQPFRQEDWNRAKNAGLEKLQQKKYAAARIASSEARVDPDEHQADLAVRYDSGPAFTLG 202 GQPFRQ DW +AK+ GL+ L +K+Y AA++A SEARV P+++ A+L+V+YDSGP FTLG Sbjct: 148 AGQPFRQADWAKAKDDGLQVLLKKRYPAAKVAHSEARVTPEDNDAELSVQYDSGPPFTLG 207 Query: 203 PLQITGTRRYPESIIRNVNPLTVGEPYDVNRLLALQRQIQNTPYFSNAIVAIDDDPEHPE 262 L ITGT+RYP SII NVNPL VGE Y V+RLL LQRQIQNTPYF N IV+I+DDP H Sbjct: 208 ALHITGTKRYPASIIENVNPLQVGEEYSVDRLLLLQRQIQNTPYFGNVIVSINDDPAHAL 267 Query: 263 QTPVKVQVTEFPTQRVRAGVGYSTDTGAQVEGRYSHYNVFNKAYVFDSQLRLEQKRQYGV 322 ++PV VQVTEF TQR+R G+GY++DTGAQV+GRY++YNVF KA+VFD+Q ++EQ+RQYG Sbjct: 268 ESPVNVQVTEFQTQRLRTGLGYASDTGAQVQGRYTNYNVFGKAWVFDAQTKIEQRRQYGA 327 Query: 323 LSLAMPPDDKAFVNSINTSYDRTKLEGIDLRSQQVGFKRARNSENYDTTYSLTYYRDELS 382 L L+MPPD ++FVN IN SYDRT L+G+DLRS ++G KRAR+ ENYDT +L YYRD L Sbjct: 328 LDLSMPPDKRSFVNGINGSYDRTTLQGVDLRSMKIGLKRARSLENYDTALTLDYYRDRLE 387 Query: 383 QEDGSTLPANTIVTPGKHRALVPGFAWSRRNVDDPIFPRKGNLLTLEAGFALKGLLTDQS 442 Q DG+TLP +T+V PG+H+ALVPGFAW RR VD+PIFPR G++ +++ GFA+KG LTDQ+ Sbjct: 388 QTDGATLPPDTVVQPGQHQALVPGFAWVRRAVDNPIFPRSGHIFSVQTGFAVKGFLTDQT 447 Query: 443 FSRLYGRFKQFVPVGKRDIVVLRAELGGVFTAGSAGAVPPSLLFRTGGNDSVRGYSYQSI 502 F R GR+K + PV KRD+V+LR ELGGVFTAGS+ AVP SLLFR GGN+SVRGYSY SI Sbjct: 448 FFRADGRYKHYFPVAKRDVVILRTELGGVFTAGSSAAVPASLLFRAGGNESVRGYSYDSI 507 Query: 503 GNEQNGTVYPTKYLAVGSSEYQRWFSENWGAAVFYDVGAAADNWNNKTFYHGVGTGARWR 562 GN QNGTVYPTKY+ GS+EYQ W ++ WG AVFYDVG A D+W ++ G G G R+R Sbjct: 508 GNSQNGTVYPTKYMVTGSAEYQHWLTQQWGGAVFYDVGTATDSWADRATKVGTGVGVRYR 567 Query: 563 SPVGTLNLDLAYGIQKRQIRPHISLGIAF 591 SPVGT+N+DLAYG+Q +Q RPHISLGIAF Sbjct: 568 SPVGTVNVDLAYGVQAKQFRPHISLGIAF 596 >ref|YP_001900156.1| surface antigen (D15) [Ralstonia pickettii 12J] ref|ZP_07676298.1| outer membrane protein, OMP85 family [Ralstonia sp. 5_7_47FAA] gb|ACD27724.1| surface antigen (D15) [Ralstonia pickettii 12J] gb|EFP65330.1| outer membrane protein, OMP85 family [Ralstonia sp. 5_7_47FAA] Length = 595 Score = 622 bits (1605), Expect = e-176, Method: Composition-based stats. Identities = 315/580 (54%), Positives = 414/580 (71%), Gaps = 18/580 (3%) Query: 12 LFCAADTAHAAYKVQIDAPKPVQSLLKDFLDISRYQDRDDISDEQLKFMMDTAGDQVREL 71 L CAA AHA Y V+IDAPK ++ LL+ LD+SRY+DR DI+D+QL++M++T GDQV +L Sbjct: 34 LACAA--AHAEYDVKIDAPKSIRDLLERHLDLSRYKDRKDITDDQLQYMVETIGDQVTKL 91 Query: 72 TSTEGYFTPVTKVSAQTEAGAEGKQAVRRITISVDPGPRTEISSAEVSVTGAAARDDQAT 131 T+TEGYF P + A +G R + + V+PGPRT I + + TGA +D + Sbjct: 92 TATEGYFVP------KATAKLDGPPDHRVVNVKVEPGPRTTIDTVALDFTGAITQDAK-R 144 Query: 132 VARLREDWSLPVGQPFRQEDWNRAKNAGLEKLQQKKYAAARIASSEARVDPDEHQADLAV 191 + L++ WSL G FRQ W++AK+ GL LQ K+Y AAR SS+ARVDPDE++ADL+V Sbjct: 145 IDELKKSWSLQHGMAFRQSGWDKAKDDGLAALQSKRYYAARQTSSQARVDPDENKADLSV 204 Query: 192 RYDSGPAFTLGPLQITGTRRYPESIIRNVNPLTVGEPYDVNRLLALQRQIQNTPYFSNAI 251 YDSGPA+TLGPL++ G RYP SII +VNPL VGE Y +RL +LQ IQ PYFSNAI Sbjct: 205 GYDSGPAYTLGPLEVQGLSRYPLSIINHVNPLRVGEDYSADRLQSLQAAIQGQPYFSNAI 264 Query: 252 VAIDDDPEHPEQTPVKVQVTEFPTQRVRAGVGYSTDTGAQVEGRYSHYNVFNKAYVFDSQ 311 V + DDP++P PV+V+V E+P R+ GVGY TDTGA +EGRY + N+F+KA+V D+Q Sbjct: 265 VDLGDDPKNPVNAPVRVRVREYPPNRITGGVGYGTDTGASLEGRYQYLNLFSKAWVLDTQ 324 Query: 312 LRLEQKRQYGVLSLAMPPDDKAFVNSINTSYDRTKLEGIDLRSQQVGFKRARNSENYDTT 371 R+EQ+RQY + +PPDDK +VNS TS DRT G D RS + GFK+ R Y+T+ Sbjct: 325 ARIEQRRQYLFAGVTVPPDDKQYVNSFYTSMDRTDTSGTDTRSYRSGFKQTRVRGIYETS 384 Query: 372 YSLTYYRDELSQEDGSTLPANTIVTPGKHRALVPGFAWSRRNVDDPIFPRKGNLLTLEAG 431 +++ +Y D+L + DG+ + +ALVPGFAW+RR+VDDP+FPR+GN++T + G Sbjct: 385 FTVDFYYDDL-RPDGAERQLS--------KALVPGFAWTRRDVDDPLFPRRGNIITTQIG 435 Query: 432 FALKGLLTDQSFSRLYGRFKQFVPVGKRDIVVLRAELGGVFTAGSAGAVPPSLLFRTGGN 491 A+KG+LTDQSF R Y R +QFVPVG+RDI V R ELG V TAGS VP +L FRTGG Sbjct: 436 AAVKGVLTDQSFVRSYSRIRQFVPVGQRDIFVARGELGAVITAGSGDGVPATLRFRTGGT 495 Query: 492 DSVRGYSYQSIGNEQNGTVYPTKYLAVGSSEYQRWFSENWGAAVFYDVGAAADNWNNKTF 551 SVRGY +QSIGN+ NG+ PTK+L G EYQRWF WGAAVF+D G A DNW+ + + Sbjct: 496 QSVRGYDFQSIGNDVNGSTLPTKFLVTGGLEYQRWFLPQWGAAVFWDTGTATDNWSERRW 555 Query: 552 YHGVGTGARWRSPVGTLNLDLAYGIQKRQIRPHISLGIAF 591 ++GVG G RW+SPVG + LD+AYGIQ++Q RP +SLGIAF Sbjct: 556 FNGVGVGVRWKSPVGPIQLDMAYGIQQKQFRPSVSLGIAF 595 >ref|NP_520505.1| lipoprotein [Ralstonia solanacearum GMI1000] emb|CAD16091.1| putative outer membrane protein [Ralstonia solanacearum GMI1000] Length = 589 Score = 620 bits (1599), Expect = e-175, Method: Composition-based stats. Identities = 316/580 (54%), Positives = 414/580 (71%), Gaps = 16/580 (2%) Query: 12 LFCAADTAHAAYKVQIDAPKPVQSLLKDFLDISRYQDRDDISDEQLKFMMDTAGDQVREL 71 L A A AAY+V+I+AP+P++ LL+ LD++RYQDR DI+D+QL++M++T G+QV +L Sbjct: 26 LSVACTAARAAYEVRIEAPRPLRDLLERHLDLARYQDRKDITDDQLQYMVETIGEQVTKL 85 Query: 72 TSTEGYFTPVTKVSAQTEAGAEGKQAVRRITISVDPGPRTEISSAEVSVTGAAARDDQAT 131 T+TEGYF P K +AQ E G R + + V+ GPRT I S + TGA A++ + Sbjct: 86 TATEGYFVP--KATAQLE----GPPERRTVRVKVEAGPRTTIDSVALDFTGAIAQEPKRI 139 Query: 132 VARLREDWSLPVGQPFRQEDWNRAKNAGLEKLQQKKYAAARIASSEARVDPDEHQADLAV 191 A L++ W LP G PFRQ W++AK L LQ K+Y AAR +S+ARVDPDE++ADL+V Sbjct: 140 DA-LKKAWGLPQGAPFRQSGWDKAKEDSLAALQSKRYYAARQTASQARVDPDENKADLSV 198 Query: 192 RYDSGPAFTLGPLQITGTRRYPESIIRNVNPLTVGEPYDVNRLLALQRQIQNTPYFSNAI 251 YDSGPA+TLGPL + G RYP SII +VNPL VGE Y +RL AL IQ PYF+NAI Sbjct: 199 AYDSGPAYTLGPLDVQGLSRYPRSIIDHVNPLIVGEDYSADRLQALAAAIQGQPYFANAI 258 Query: 252 VAIDDDPEHPEQTPVKVQVTEFPTQRVRAGVGYSTDTGAQVEGRYSHYNVFNKAYVFDSQ 311 V + DDP +P PV+V+V E+P R+ +GVGYSTDTGAQVEGRY + N+F+KA+V D+Q Sbjct: 259 VDLGDDPNNPVAAPVRVRVREYPPNRITSGVGYSTDTGAQVEGRYQYLNLFDKAWVLDTQ 318 Query: 312 LRLEQKRQYGVLSLAMPPDDKAFVNSINTSYDRTKLEGIDLRSQQVGFKRARNSENYDTT 371 R+EQ+RQY S+ +PPD K +VNS+ S DRT L G D RS + GFK+ R Y+T+ Sbjct: 319 ARIEQRRQYLFGSVTLPPDGKRYVNSLYGSLDRTNLSGTDTRSYRSGFKQTRVRGIYETS 378 Query: 372 YSLTYYRDELSQEDGSTLPANTIVTPGKHRALVPGFAWSRRNVDDPIFPRKGNLLTLEAG 431 +++ +Y D+L E + + +ALVPGFAW+RR+VDDP+FPR+GN+LT + G Sbjct: 379 FTIDFYYDDLRPEGAARELS---------KALVPGFAWTRRDVDDPLFPRRGNILTTQIG 429 Query: 432 FALKGLLTDQSFSRLYGRFKQFVPVGKRDIVVLRAELGGVFTAGSAGAVPPSLLFRTGGN 491 A+KGLLTDQSF R Y R +Q+VPVG+RDI V RAELG V TAG+A VP +L FRTGG Sbjct: 430 AAVKGLLTDQSFVRSYSRIRQYVPVGQRDIFVARAELGAVLTAGAADGVPATLRFRTGGT 489 Query: 492 DSVRGYSYQSIGNEQNGTVYPTKYLAVGSSEYQRWFSENWGAAVFYDVGAAADNWNNKTF 551 S+RGY +QSIGN+ NG+ PTK+L G EYQRWF WGAAVF+D G A DNW + + Sbjct: 490 QSIRGYDFQSIGNDVNGSTLPTKFLVTGGVEYQRWFLPQWGAAVFWDTGTATDNWTQRRW 549 Query: 552 YHGVGTGARWRSPVGTLNLDLAYGIQKRQIRPHISLGIAF 591 ++GVG G RW+SPVG + LDLAYGIQ+ Q RP ++LGIAF Sbjct: 550 FNGVGVGVRWKSPVGPIQLDLAYGIQQHQFRPSVALGIAF 589 >emb|CBJ37198.1| Conserved protein of unknown function; similar to D15 or Oma87 antigen [Ralstonia solanacearum CMR15] Length = 589 Score = 619 bits (1596), Expect = e-175, Method: Composition-based stats. Identities = 317/580 (54%), Positives = 416/580 (71%), Gaps = 16/580 (2%) Query: 12 LFCAADTAHAAYKVQIDAPKPVQSLLKDFLDISRYQDRDDISDEQLKFMMDTAGDQVREL 71 L A A AAY+V+I+AP+P++ LL LD++RYQDR DI+D+QL++M++T G+QV +L Sbjct: 26 LSVACTAARAAYEVRIEAPRPLRDLLARHLDLARYQDRKDITDDQLQYMVETIGEQVAKL 85 Query: 72 TSTEGYFTPVTKVSAQTEAGAEGKQAVRRITISVDPGPRTEISSAEVSVTGAAARDDQAT 131 T+TEGYF P K +AQ E G R + + V+ GPRT I S + TGA A++ + Sbjct: 86 TATEGYFVP--KATAQLE----GPPDRRIVRVKVEAGPRTTIDSVALDFTGAIAQEPKRI 139 Query: 132 VARLREDWSLPVGQPFRQEDWNRAKNAGLEKLQQKKYAAARIASSEARVDPDEHQADLAV 191 A L++ W LP G PFRQ W++AK L LQ K+Y AAR +S+ARVDPDE++ADL+V Sbjct: 140 DA-LKKAWGLPQGAPFRQSGWDKAKEDSLAALQSKRYYAARQTASQARVDPDENKADLSV 198 Query: 192 RYDSGPAFTLGPLQITGTRRYPESIIRNVNPLTVGEPYDVNRLLALQRQIQNTPYFSNAI 251 YDSGPA+TLGPL + G RYP SII +VNPL VGE Y +RL AL IQ PYF+NAI Sbjct: 199 AYDSGPAYTLGPLDVQGLSRYPRSIIDHVNPLIVGEDYSADRLQALAAAIQGQPYFANAI 258 Query: 252 VAIDDDPEHPEQTPVKVQVTEFPTQRVRAGVGYSTDTGAQVEGRYSHYNVFNKAYVFDSQ 311 V + DDP++P PV+V+V E+P R+ +GVGYSTDTGAQVEGRY + N+F+KA+V D+Q Sbjct: 259 VDLGDDPKNPVAAPVRVRVREYPPNRITSGVGYSTDTGAQVEGRYQYLNLFDKAWVLDTQ 318 Query: 312 LRLEQKRQYGVLSLAMPPDDKAFVNSINTSYDRTKLEGIDLRSQQVGFKRARNSENYDTT 371 R+EQ+RQY S+ +PPD K +VNS+ S DRT L G D RS + GFK+ R Y+T+ Sbjct: 319 ARIEQRRQYLFGSVTLPPDGKRYVNSLYGSLDRTNLSGTDTRSYRSGFKQTRARGIYETS 378 Query: 372 YSLTYYRDELSQEDGSTLPANTIVTPGKHRALVPGFAWSRRNVDDPIFPRKGNLLTLEAG 431 +++ +Y D+L + DG+ + +ALVPGFAW+RR+VDDP+FPR+GN+LT + G Sbjct: 379 FTIDFYYDDL-RPDGAARELS--------KALVPGFAWTRRDVDDPLFPRRGNILTTQIG 429 Query: 432 FALKGLLTDQSFSRLYGRFKQFVPVGKRDIVVLRAELGGVFTAGSAGAVPPSLLFRTGGN 491 A+KGLLTDQSF R Y R +Q+VPVG+RDI V RAELG V TAG+A VP +L FRTGG Sbjct: 430 AAVKGLLTDQSFVRSYSRIRQYVPVGQRDIFVARAELGAVLTAGAADGVPATLRFRTGGT 489 Query: 492 DSVRGYSYQSIGNEQNGTVYPTKYLAVGSSEYQRWFSENWGAAVFYDVGAAADNWNNKTF 551 S+RGY +QSIGN+ NG+ PTK+L G EYQRWF WGAAVF+D G A DNW + + Sbjct: 490 QSIRGYDFQSIGNDVNGSTLPTKFLITGGVEYQRWFLPQWGAAVFWDTGTATDNWTQRRW 549 Query: 552 YHGVGTGARWRSPVGTLNLDLAYGIQKRQIRPHISLGIAF 591 ++GVG G RW+SPVG + LDLAYGIQ+ Q RP ++LGIAF Sbjct: 550 FNGVGVGVRWKSPVGPIQLDLAYGIQQHQFRPSVALGIAF 589