BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2878.p (395 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774912.1| naphthocyclinone hydroxylase [Herbaspirillum... 719 0.0 ref|YP_001889811.1| acyl-CoA dehydrogenase type 2 domain [Burkho... 467 e-129 ref|ZP_06844479.1| Acyl-CoA dehydrogenase type 2 domain [Burkhol... 462 e-128 ref|YP_553029.1| hypothetical protein Bxe_B2308 [Burkholderia xe... 461 e-127 gb|EGD01087.1| putative hydroxylase [Burkholderia sp. TJI49] 455 e-126 >ref|YP_003774912.1| naphthocyclinone hydroxylase [Herbaspirillum seropedicae SmR1] gb|ADJ63004.1| naphthocyclinone hydroxylase protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 719 bits (1857), Expect = 0.0, Method: Composition-based stats. Identities = 364/395 (92%), Positives = 381/395 (96%), Gaps = 1/395 (0%) Query: 1 MNSITLDVYPVQKKIVTAAPPHADSPEMDTLIAEVTARRDEFDQLGHVPRDLVATMKKAG 60 MNSITLDVYPVQKK+V A PPH DSPEMDTLIAEVTARRDEFDQLGHVPRDL+ATMK+AG Sbjct: 1 MNSITLDVYPVQKKVVAALPPHVDSPEMDTLIAEVTARRDEFDQLGHVPRDLIATMKQAG 60 Query: 61 IFRSGTPRKFGGEALAPAPFLAMVERISKADGSAGWVAAFGSANTYLAALPIETQRKIYA 120 IFRS TPR+FGGE LAPAPFLAMVERIS+ADGSAGWVAAFGSANTYLAALPIETQR+IYA Sbjct: 61 IFRSSTPRRFGGEPLAPAPFLAMVERISRADGSAGWVAAFGSANTYLAALPIETQRQIYA 120 Query: 121 SGPDQVYAGGLYPLQEAKAVPGGWEITGRWHFASGCKGADWIGVGIKDSSGADDGTPKPA 180 GPDQVYAGGLYPLQEAK VPGGW I+GRWHFASGCKGADWIGVGIKD G D+G P+PA Sbjct: 121 DGPDQVYAGGLYPLQEAKPVPGGWNISGRWHFASGCKGADWIGVGIKDG-GGDEGAPRPA 179 Query: 181 LMAVMPASDIEIIDNWNVVGMQGTGSHDTRLKDKFVAHEWTCARGAAGLIDEPLYRYPAL 240 LMAVMPA+++EIIDNWNVVGMQGTGSHDTRLKDKFVAHEWTCARGA GLIDEPLYRYPAL Sbjct: 180 LMAVMPAAEVEIIDNWNVVGMQGTGSHDTRLKDKFVAHEWTCARGAPGLIDEPLYRYPAL 239 Query: 241 AYQAEVHAAVNIGLARAALDIVTDMAGGAKIMPGALRLADRAYYKIKLGLSEAKWRSARM 300 AYQAEVHAAVNIGLARAALDIVTDMAGGAKIMPGALRLADRAYYKIKLGLSEAKWRSARM Sbjct: 240 AYQAEVHAAVNIGLARAALDIVTDMAGGAKIMPGALRLADRAYYKIKLGLSEAKWRSARM 299 Query: 301 FFYDACEQAWEQIVAGNPVTEEIDMMLKLSATYAARVSMEVVNDAYQAAGMAAIQRTHRL 360 FFYDACEQAWEQI+AGNP++EE DMMLKLSATYAARVSMEVVNDAYQAAGMAAIQR+HRL Sbjct: 300 FFYDACEQAWEQILAGNPISEETDMMLKLSATYAARVSMEVVNDAYQAAGMAAIQRSHRL 359 Query: 361 QRIVRDAMVVTQHAALSELNYESAGALFAKLGIKR 395 QRIVRDAMVVTQHAALSELNYESAGALFAKLGI+R Sbjct: 360 QRIVRDAMVVTQHAALSELNYESAGALFAKLGIRR 394 >ref|YP_001889811.1| acyl-CoA dehydrogenase type 2 domain [Burkholderia phytofirmans PsJN] gb|ACD20440.1| Acyl-CoA dehydrogenase type 2 domain [Burkholderia phytofirmans PsJN] Length = 405 Score = 467 bits (1201), Expect = e-129, Method: Composition-based stats. Identities = 238/372 (63%), Positives = 294/372 (79%), Gaps = 3/372 (0%) Query: 20 PPHADSPEMDTLIAEVTARRDEFDQLGHVPRDLVATMKKAGIFRSGTPRKFGGEALAPAP 79 P +A +D LI E+ RRDEFD L HVPRD++A MK+AGIFR+ TP++FGG+AL PA Sbjct: 26 PDYAGPLTLDALIGEIERRRDEFDHLSHVPRDMIAKMKRAGIFRASTPQRFGGDALPPAQ 85 Query: 80 FLAMVERISKADGSAGWVAAFGSANTYLAALPIETQRKIYASGPDQVYAGGLYPLQEAKA 139 FL M+ERI+ ADGSA WVAAFGSANTYLAALPIETQ++IYA+GPDQV++GGLYPLQ A Sbjct: 86 FLRMLERIAIADGSAAWVAAFGSANTYLAALPIETQQQIYATGPDQVFSGGLYPLQPATK 145 Query: 140 VPGGWEITGRWHFASGCKGADWIGVGI--KDSSGADDGTPKPALMAVMPASDIEIIDNWN 197 PGG+ ++G+W FASGCKGADWIGVGI ++ D G KP AV PA ++EI++NWN Sbjct: 146 APGGFVVSGQWRFASGCKGADWIGVGIGGTPANSGDAGAGKP-FTAVFPAQEVEIVENWN 204 Query: 198 VVGMQGTGSHDTRLKDKFVAHEWTCARGAAGLIDEPLYRYPALAYQAEVHAAVNIGLARA 257 VVGMQGTGSHD RL++KFV +WT RG A LIDEPLYRYPA+AYQA+VHAAVNIGLARA Sbjct: 205 VVGMQGTGSHDLRLREKFVDAQWTFVRGGAALIDEPLYRYPAVAYQAQVHAAVNIGLARA 264 Query: 258 ALDIVTDMAGGAKIMPGALRLADRAYYKIKLGLSEAKWRSARMFFYDACEQAWEQIVAGN 317 ALD++TDM+G K GA RLADR YY+ L +EA WRSAR FFY++ E AW I+ G+ Sbjct: 265 ALDLLTDMSGSTKTTTGAPRLADRGYYRSGLAKAEANWRSARAFFYESAEAAWRTIIDGD 324 Query: 318 PVTEEIDMMLKLSATYAARVSMEVVNDAYQAAGMAAIQRTHRLQRIVRDAMVVTQHAALS 377 V++E +L+LSAT+AA++ EVV AYQ AG+AAI R +RLQR+VRD++VVTQHA L Sbjct: 325 AVSQEQANLLRLSATHAAQIGAEVVMQAYQMAGIAAIYRENRLQRLVRDSIVVTQHAFLG 384 Query: 378 ELNYESAGALFA 389 E Y+++GALFA Sbjct: 385 EGTYDASGALFA 396 >ref|ZP_06844479.1| Acyl-CoA dehydrogenase type 2 domain [Burkholderia sp. Ch1-1] gb|EFG67966.1| Acyl-CoA dehydrogenase type 2 domain [Burkholderia sp. Ch1-1] Length = 405 Score = 462 bits (1188), Expect = e-128, Method: Composition-based stats. Identities = 237/372 (63%), Positives = 293/372 (78%), Gaps = 3/372 (0%) Query: 20 PPHADSPEMDTLIAEVTARRDEFDQLGHVPRDLVATMKKAGIFRSGTPRKFGGEALAPAP 79 P +A +D LI E+ RRDEFDQL HVPRD++A MK+AGIFR+ TP++FGG+AL PA Sbjct: 26 PDYAGPITLDALIGEIERRRDEFDQLSHVPRDMIAKMKRAGIFRASTPQRFGGDALPPAQ 85 Query: 80 FLAMVERISKADGSAGWVAAFGSANTYLAALPIETQRKIYASGPDQVYAGGLYPLQEAKA 139 FL ++ERI+ ADGSA WVAAFGSANTYLAALPIETQ++IYA+GPDQV++GGLYPLQ A Sbjct: 86 FLRILERIALADGSAAWVAAFGSANTYLAALPIETQQQIYATGPDQVFSGGLYPLQPANP 145 Query: 140 VPGGWEITGRWHFASGCKGADWIGVGIKDSSGADDGTP--KPALMAVMPASDIEIIDNWN 197 PGG+ ++G+W FASGCKGADWIGVGI + G KP AV+PA ++EI++NWN Sbjct: 146 APGGFLVSGQWRFASGCKGADWIGVGIGGTPANAGGAAAGKP-FTAVLPAHEVEIVENWN 204 Query: 198 VVGMQGTGSHDTRLKDKFVAHEWTCARGAAGLIDEPLYRYPALAYQAEVHAAVNIGLARA 257 VVGMQGTGSHD RL++KFV +WT RG A LIDEPLYRYPA+AYQA+VHAAVNIGLARA Sbjct: 205 VVGMQGTGSHDLRLREKFVDAQWTFVRGGAALIDEPLYRYPAIAYQAQVHAAVNIGLARA 264 Query: 258 ALDIVTDMAGGAKIMPGALRLADRAYYKIKLGLSEAKWRSARMFFYDACEQAWEQIVAGN 317 ALD++T M+G K GA RLADR YY+ L +EA WRSAR FFY++ E AW+ I+ G+ Sbjct: 265 ALDLLTGMSGSTKTTTGAPRLADRGYYRSGLAKAEASWRSARAFFYESAETAWQTIIDGD 324 Query: 318 PVTEEIDMMLKLSATYAARVSMEVVNDAYQAAGMAAIQRTHRLQRIVRDAMVVTQHAALS 377 V++E +L+LSAT+AA+V EVV AYQ AG+AAI R RLQR+VRD++VVTQHA L Sbjct: 325 AVSQEQANLLRLSATHAAQVGAEVVMQAYQMAGIAAIYRESRLQRLVRDSIVVTQHAFLG 384 Query: 378 ELNYESAGALFA 389 E Y+++GALFA Sbjct: 385 EGTYDASGALFA 396 >ref|YP_553029.1| hypothetical protein Bxe_B2308 [Burkholderia xenovorans LB400] gb|ABE33679.1| Conserved hypothetical protein [Burkholderia xenovorans LB400] Length = 405 Score = 461 bits (1185), Expect = e-127, Method: Composition-based stats. Identities = 237/374 (63%), Positives = 291/374 (77%), Gaps = 7/374 (1%) Query: 20 PPHADSPEMDTLIAEVTARRDEFDQLGHVPRDLVATMKKAGIFRSGTPRKFGGEALAPAP 79 P +A +D L E+ RRDEFD L HVPRD++A MK+AGIFR+ TP++FGG+AL PA Sbjct: 26 PDYAGPITLDALTGEIERRRDEFDHLSHVPRDMIAKMKRAGIFRASTPQRFGGDALPPAQ 85 Query: 80 FLAMVERISKADGSAGWVAAFGSANTYLAALPIETQRKIYASGPDQVYAGGLYPLQEAKA 139 FL M+ERI+ ADGSA WVAAFGSANTYLAALPIETQ++IYA+GPDQV++GGLYPLQ A Sbjct: 86 FLRMLERIAIADGSAAWVAAFGSANTYLAALPIETQQQIYATGPDQVFSGGLYPLQPAIP 145 Query: 140 VPGGWEITGRWHFASGCKGADWIGVGI----KDSSGADDGTPKPALMAVMPASDIEIIDN 195 PGG+ ++G+W FASGCKGADWIGVGI +S GA G P AV PA ++EI++N Sbjct: 146 APGGFLVSGQWRFASGCKGADWIGVGIGGTPANSGGAAAGKP---FTAVFPAHEVEIVEN 202 Query: 196 WNVVGMQGTGSHDTRLKDKFVAHEWTCARGAAGLIDEPLYRYPALAYQAEVHAAVNIGLA 255 WNVVGMQGTGSHD RL+DKFV +WT RG LIDEPLYRYPA+AYQA+VHAAVNIGLA Sbjct: 203 WNVVGMQGTGSHDLRLRDKFVDAQWTFVRGGVALIDEPLYRYPAIAYQAQVHAAVNIGLA 262 Query: 256 RAALDIVTDMAGGAKIMPGALRLADRAYYKIKLGLSEAKWRSARMFFYDACEQAWEQIVA 315 RAALD++T M+G K GA RLADR YY+ L +EA WRSAR FFY++ E AW+ I+ Sbjct: 263 RAALDLLTGMSGSTKTTTGAPRLADRGYYRSGLAKAEASWRSARAFFYESAEAAWQTIID 322 Query: 316 GNPVTEEIDMMLKLSATYAARVSMEVVNDAYQAAGMAAIQRTHRLQRIVRDAMVVTQHAA 375 G+ V++E +L+LSAT+AA++ EVV AYQ AG+AAI R RLQR+VRD++VVTQHA Sbjct: 323 GDAVSQEQANLLRLSATHAAQIGAEVVMQAYQMAGIAAIYRESRLQRLVRDSIVVTQHAF 382 Query: 376 LSELNYESAGALFA 389 L E Y+++GALFA Sbjct: 383 LGEGTYDASGALFA 396 >gb|EGD01087.1| putative hydroxylase [Burkholderia sp. TJI49] Length = 396 Score = 455 bits (1170), Expect = e-126, Method: Composition-based stats. Identities = 230/370 (62%), Positives = 288/370 (77%), Gaps = 1/370 (0%) Query: 20 PPHADSPEMDTLIAEVTARRDEFDQLGHVPRDLVATMKKAGIFRSGTPRKFGGEALAPAP 79 P +A+ +D LI EV RRDEFD L HVPRD+V MK+AGIFR+ TP++FGG+AL PA Sbjct: 17 PAYAEPLSLDALIEEVERRRDEFDALSHVPRDMVGKMKRAGIFRASTPKRFGGDALPPAR 76 Query: 80 FLAMVERISKADGSAGWVAAFGSANTYLAALPIETQRKIYASGPDQVYAGGLYPLQEAKA 139 FL M+ERI+ ADGS WVAAFGSANTYLA+LPIETQ +IYA+GPDQV+AGGLYPLQ+A+ Sbjct: 77 FLEMLERIAIADGSTAWVAAFGSANTYLASLPIETQAQIYATGPDQVFAGGLYPLQKAER 136 Query: 140 VPGGWEITGRWHFASGCKGADWIGVGIKDS-SGADDGTPKPALMAVMPASDIEIIDNWNV 198 PGG+ ++G+W FASGCKGADWIGVGI + +GADD AV PAS++EI+DNWNV Sbjct: 137 APGGFLVSGQWRFASGCKGADWIGVGIGGAPAGADDKNAGKPFTAVFPASEVEIVDNWNV 196 Query: 199 VGMQGTGSHDTRLKDKFVAHEWTCARGAAGLIDEPLYRYPALAYQAEVHAAVNIGLARAA 258 VGMQGTGSHD RLKDK+V WT RG LIDEPLYRYPA+AYQA+VHAAVNIGLARAA Sbjct: 197 VGMQGTGSHDLRLKDKYVEERWTFVRGGTPLIDEPLYRYPAVAYQAQVHAAVNIGLARAA 256 Query: 259 LDIVTDMAGGAKIMPGALRLADRAYYKIKLGLSEAKWRSARMFFYDACEQAWEQIVAGNP 318 LD++ M+G GA RLADRAYY+ L +EA+ RSAR FF++A E +W ++AG+P Sbjct: 257 LDMLAGMSGVTTTTTGAPRLADRAYYRSGLAQAEAQLRSARAFFFEAAEASWRTVLAGDP 316 Query: 319 VTEEIDMMLKLSATYAARVSMEVVNDAYQAAGMAAIQRTHRLQRIVRDAMVVTQHAALSE 378 V+ +L+LSAT+AA ++V AY+ AG+ AI R +R+QR+VRD++VVTQHA L E Sbjct: 317 VSPSGANLLRLSATHAAHTCADIVMQAYKMAGIGAIYRENRMQRLVRDSIVVTQHAFLGE 376 Query: 379 LNYESAGALF 388 NY+++GA+F Sbjct: 377 GNYDASGAIF 386