BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2621.p (360 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAR97453.1| nitrilase [uncultured organism] 541 e-152 gb|AAR97497.1| nitrilase [uncultured organism] 486 e-135 ref|YP_002541023.1| nitrilase [Agrobacterium radiobacter K84] >g... 483 e-134 ref|ZP_08782577.1| Nitrilase [Methylobacter tundripaludum SV96] ... 482 e-134 ref|YP_004348430.1| aliphatic nitrilase [Burkholderia gladioli B... 477 e-132 >gb|AAR97453.1| nitrilase [uncultured organism] Length = 349 Score = 541 bits (1395), Expect = e-152, Method: Composition-based stats. Identities = 276/346 (79%), Positives = 295/346 (85%), Gaps = 4/346 (1%) Query: 1 MSMHKRTVRAAAVQIAPDLDSEHGTVAKVCQAIADAAAQGAQLVVFPETFVPYYPYFSFV 60 M+ K+TVRAAA+QIAPDLDS GT+ KVC AI AAAQGA+LVVFPETF+PYYPYFSFV Sbjct: 1 MTTVKKTVRAAAIQIAPDLDSAGGTLDKVCTAIQKAAAQGAELVVFPETFLPYYPYFSFV 60 Query: 61 RPPFASGPEHLLLYERAVTVPGPVTQAVSAAARQHGTVVVLGVNERDHGTLYNAQLVFDA 120 RPPFASGPEHLLLYERAV VPGPVT AVSA AR HG VVVLGVNERDHGTLYN QLVFDA Sbjct: 61 RPPFASGPEHLLLYERAVAVPGPVTDAVSAVARSHGVVVVLGVNERDHGTLYNTQLVFDA 120 Query: 121 DGTLVLKRRKITPTYHERMIWGQGDASGLKVVPTAVGRLGALACWEHYNPLARYSLMAQH 180 +G LVLKRRKITPTYHERMIWGQGD SGLKVV TAVGRLGALACWEHYNPLARY+LMAQH Sbjct: 121 NGELVLKRRKITPTYHERMIWGQGDGSGLKVVQTAVGRLGALACWEHYNPLARYALMAQH 180 Query: 181 EEIHCAQFPGSMVGQIFADQMEVTIRHHALESGCFVINATGWLSDEQIAAIEPDPQAQKA 240 EEIHCAQFPGSMVGQIFADQMEVTIRHHALES CFV+NATGWL+D QI +I PDP QKA Sbjct: 181 EEIHCAQFPGSMVGQIFADQMEVTIRHHALESACFVVNATGWLTDAQITSITPDPALQKA 240 Query: 241 LRGGCCTAIVSPEGKLLAEPLRSGEGILVADLDMELVTKRKRMMDSVGHYARPELLHLVI 300 LRGGCCTAIVSPEG LLAEPLRSGEG+++ADLDM L+TKRKRMMDSVGHYARPELL L++ Sbjct: 241 LRGGCCTAIVSPEGVLLAEPLRSGEGMVIADLDMALITKRKRMMDSVGHYARPELLSLLV 300 Query: 301 DDRRTSPMSSLFPAEQPA---PVTHPSARFPHADSRHPVASPAPEP 343 DDRR P+S+LF PA V PS P S H S EP Sbjct: 301 DDRRKVPVSALFADSNPANGHTVFTPS-DIPTLGSAHHANSYQTEP 345 >gb|AAR97497.1| nitrilase [uncultured organism] Length = 344 Score = 486 bits (1250), Expect = e-135, Method: Composition-based stats. Identities = 235/311 (75%), Positives = 266/311 (85%) Query: 5 KRTVRAAAVQIAPDLDSEHGTVAKVCQAIADAAAQGAQLVVFPETFVPYYPYFSFVRPPF 64 +R VRAAA+QIAPDL+ T+ KVC AI +AA +GAQL VFPETFVPYYPYFSFVRPP Sbjct: 4 RRIVRAAAIQIAPDLERCSVTLEKVCSAIDEAAGKGAQLSVFPETFVPYYPYFSFVRPPV 63 Query: 65 ASGPEHLLLYERAVTVPGPVTQAVSAAARQHGTVVVLGVNERDHGTLYNAQLVFDADGTL 124 ASG +H+ LYE AV VPGPVTQAVS AR HG VVVLGVNERDHG+LYN QL+FD DG L Sbjct: 64 ASGADHMRLYEEAVVVPGPVTQAVSERARMHGMVVVLGVNERDHGSLYNTQLIFDCDGRL 123 Query: 125 VLKRRKITPTYHERMIWGQGDASGLKVVPTAVGRLGALACWEHYNPLARYSLMAQHEEIH 184 LKRRKITPT+HERMIWGQGDASGLKV T +GR+GALACWEHYNPLARY+LM QHEEIH Sbjct: 124 ALKRRKITPTFHERMIWGQGDASGLKVARTGIGRVGALACWEHYNPLARYALMTQHEEIH 183 Query: 185 CAQFPGSMVGQIFADQMEVTIRHHALESGCFVINATGWLSDEQIAAIEPDPQAQKALRGG 244 C+QFPGS+VG IF++QM+VTIRHHALESGCFV+NATGWL+D QIA+I DP+ Q+ALRGG Sbjct: 184 CSQFPGSLVGPIFSEQMDVTIRHHALESGCFVVNATGWLTDAQIASITDDPKLQRALRGG 243 Query: 245 CCTAIVSPEGKLLAEPLRSGEGILVADLDMELVTKRKRMMDSVGHYARPELLHLVIDDRR 304 C TAIVSPEG+ LAEPLR GEG++VADLDM L+TKRKRMMDSVGHYARPELL L I+DR Sbjct: 244 CNTAIVSPEGQHLAEPLREGEGMVVADLDMSLITKRKRMMDSVGHYARPELLSLAINDRP 303 Query: 305 TSPMSSLFPAE 315 +P + AE Sbjct: 304 AAPFGRMCAAE 314 >ref|YP_002541023.1| nitrilase [Agrobacterium radiobacter K84] gb|ACM29426.1| nitrilase [Agrobacterium radiobacter K84] Length = 350 Score = 483 bits (1242), Expect = e-134, Method: Composition-based stats. Identities = 242/316 (76%), Positives = 268/316 (84%), Gaps = 5/316 (1%) Query: 5 KRTVRAAAVQIAPDLDSEHGTVAKVCQAIADAAAQGAQLVVFPETFVPYYPYFSFVRPPF 64 K TVR AAVQIAPDL S TVA+V +AIA A+A+GA+LVVFPETFVP+YPYFSFV PP Sbjct: 4 KSTVRVAAVQIAPDLTSREKTVARVIEAIAQASAKGAELVVFPETFVPWYPYFSFVLPPV 63 Query: 65 ASGPEHLLLYERAVTVPGPVTQAVSAAARQHGTVVVLGVNERDHGTLYNAQLVFDADGTL 124 SG EHL LYE AV VP T++V+AAAR+HG VV LGVNERDHGTLYN QL+FDADG+L Sbjct: 64 LSGKEHLRLYEEAVAVPSATTRSVAAAAREHGIVVALGVNERDHGTLYNTQLLFDADGSL 123 Query: 125 VLKRRKITPTYHERMIWGQGDASGLKVVPTAVGRLGALACWEHYNPLARYSLMAQHEEIH 184 +LKRRKITPT+HERMIWGQGDASGLKVV +A+GR+GALACWEHYNPLARY+LMAQHEEIH Sbjct: 124 ILKRRKITPTFHERMIWGQGDASGLKVVDSAIGRIGALACWEHYNPLARYALMAQHEEIH 183 Query: 185 CAQFPGSMVGQIFADQMEVTIRHHALESGCFVINATGWLSDEQIAAIEPDPQAQKALRGG 244 AQFPGSMVG IFADQMEVTIRHHALESGCFV+NATGWL+D+QI +I PD QKALRGG Sbjct: 184 IAQFPGSMVGPIFADQMEVTIRHHALESGCFVVNATGWLTDDQIVSITPDTGLQKALRGG 243 Query: 245 CCTAIVSPEGKLLAEPLRSGEGILVADLDMELVTKRKRMMDSVGHYARPELLHLVIDDR- 303 C TAI+SPEGK L PL GEGILVADLDM L+ KRKRMMDSVGHYARPELLHLV+D R Sbjct: 244 CMTAIISPEGKHLVPPLTEGEGILVADLDMSLILKRKRMMDSVGHYARPELLHLVMDARP 303 Query: 304 ----RTSPMSSLFPAE 315 RTS + FP E Sbjct: 304 AAPMRTSTIPDAFPGE 319 >ref|ZP_08782577.1| Nitrilase [Methylobacter tundripaludum SV96] gb|EGW20849.1| Nitrilase [Methylobacter tundripaludum SV96] Length = 330 Score = 482 bits (1241), Expect = e-134, Method: Composition-based stats. Identities = 247/319 (77%), Positives = 275/319 (86%), Gaps = 3/319 (0%) Query: 5 KRTVRAAAVQIAPDLDSEHGTVAKVCQAIADAAAQGAQLVVFPETFVPYYPYFSFVRPPF 64 K+ VRAAAVQIAP DS GTV KVCQAI+DAA +GAQLVVFPETFVPYYPYFSFV PP Sbjct: 4 KKIVRAAAVQIAPVFDSAFGTVDKVCQAISDAAKEGAQLVVFPETFVPYYPYFSFVLPPV 63 Query: 65 ASGPEHLLLYERAVTVPGPVTQAVSAAARQHGTVVVLGVNERDHGTLYNAQLVFDADGTL 124 ASG HL LYE AV VPGPVT AV+A AR G VVVLGVNERDHG+LYN QL+FD G+L Sbjct: 64 ASGGAHLKLYEHAVVVPGPVTDAVAAQARSLGVVVVLGVNERDHGSLYNTQLIFDETGSL 123 Query: 125 VLKRRKITPTYHERMIWGQGDASGLKVVPTAVGRLGALACWEHYNPLARYSLMAQHEEIH 184 VLKRRKITPT+HERMIWGQGDASGLKVV T +GR+GALACWEHYNPLARY+LMAQHEEIH Sbjct: 124 VLKRRKITPTFHERMIWGQGDASGLKVVETGIGRIGALACWEHYNPLARYALMAQHEEIH 183 Query: 185 CAQFPGSMVGQIFADQMEVTIRHHALESGCFVINATGWLSDEQIAAIEPDPQAQKALRGG 244 C+QFPGS+VG IFA+Q+EVTIRHHALESGCFVINATGWLSD+QI ++ PDP QKALRGG Sbjct: 184 CSQFPGSLVGPIFAEQIEVTIRHHALESGCFVINATGWLSDQQIESVTPDPGLQKALRGG 243 Query: 245 CCTAIVSPEGKLLAEPLRSGEGILVADLDMELVTKRKRMMDSVGHYARPELLHLVIDDRR 304 CCTAI+SPEG+ LAEPLRSGEG+++ADLDM L+TKRKRMMDSVGHYARPELL L I+DR Sbjct: 244 CCTAIISPEGQHLAEPLRSGEGMVIADLDMSLITKRKRMMDSVGHYARPELLSLRINDRP 303 Query: 305 TSPMSSLFPAEQPAPVTHP 323 +PM F E A ++ P Sbjct: 304 AAPM---FSEESTAELSFP 319 >ref|YP_004348430.1| aliphatic nitrilase [Burkholderia gladioli BSR3] gb|AEA62918.1| aliphatic nitrilase [Burkholderia gladioli BSR3] Length = 337 Score = 477 bits (1228), Expect = e-132, Method: Composition-based stats. Identities = 235/310 (75%), Positives = 272/310 (87%) Query: 5 KRTVRAAAVQIAPDLDSEHGTVAKVCQAIADAAAQGAQLVVFPETFVPYYPYFSFVRPPF 64 K +RAAA QIAPDL T+ KVC AI +AAA+G QL+VFPETFVPYYPYFSFVRPP Sbjct: 4 KSVIRAAAAQIAPDLARAAATLDKVCAAIDEAAAKGVQLIVFPETFVPYYPYFSFVRPPV 63 Query: 65 ASGPEHLLLYERAVTVPGPVTQAVSAAARQHGTVVVLGVNERDHGTLYNAQLVFDADGTL 124 ASG EH+ LYE AV VPGPVT+AV+ AR+HG VVVLGVNERDHG+LYN QLVFDADG+L Sbjct: 64 ASGAEHMRLYEEAVVVPGPVTEAVAERARRHGMVVVLGVNERDHGSLYNTQLVFDADGSL 123 Query: 125 VLKRRKITPTYHERMIWGQGDASGLKVVPTAVGRLGALACWEHYNPLARYSLMAQHEEIH 184 +LKRRK+TPT+HERMIWGQGDA+GL+VV TAVGR+GALACWEHYNPLARY+LMAQHE+IH Sbjct: 124 LLKRRKLTPTFHERMIWGQGDAAGLRVVDTAVGRVGALACWEHYNPLARYALMAQHEQIH 183 Query: 185 CAQFPGSMVGQIFADQMEVTIRHHALESGCFVINATGWLSDEQIAAIEPDPQAQKALRGG 244 CAQFPGS+VG IFA+Q++VTIRHHALESGCFV+NATGWLSD QI +I PDP QKALRGG Sbjct: 184 CAQFPGSLVGPIFAEQIDVTIRHHALESGCFVVNATGWLSDAQIESITPDPGLQKALRGG 243 Query: 245 CCTAIVSPEGKLLAEPLRSGEGILVADLDMELVTKRKRMMDSVGHYARPELLHLVIDDRR 304 C +AIVSPEG+ LAEPLR GEG+++ADLD+ L+TKRKRMMDSVGHYARPELL L I+DR Sbjct: 244 CHSAIVSPEGQHLAEPLREGEGLVIADLDLSLITKRKRMMDSVGHYARPELLSLAINDRP 303 Query: 305 TSPMSSLFPA 314 P++++ PA Sbjct: 304 AMPVTAMAPA 313