BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2529.p (544 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_004751856.1| Penicillin amidase [Collimonas fungivorans T... 865 0.0 ref|YP_998025.1| penicillin amidase [Verminephrobacter eiseniae ... 811 0.0 ref|ZP_07673591.1| penicillin acylase (penicillin amidase) [Rals... 790 0.0 ref|YP_001892723.1| peptidase S45 penicillin amidase [Ralstonia ... 788 0.0 emb|CCA81837.1| putative penicillin G amidase precursor; peptida... 776 0.0 >ref|YP_004751856.1| Penicillin amidase [Collimonas fungivorans Ter331] gb|AEK61033.1| Penicillin amidase [Collimonas fungivorans Ter331] Length = 804 Score = 865 bits (2234), Expect = 0.0, Method: Composition-based stats. Identities = 429/541 (79%), Positives = 474/541 (87%), Gaps = 3/541 (0%) Query: 2 EGSNNWVIAPSKSATGRAIMANDPHRAYSAPSLRYIAHLSAPGMNVIGAGEPALPGISIG 61 EGSNNWV+AP+KSATGRAIMANDPHRAYSAPSLRYIAH++APG+NVIGAGEPALPGISIG Sbjct: 265 EGSNNWVVAPAKSATGRAIMANDPHRAYSAPSLRYIAHINAPGLNVIGAGEPALPGISIG 324 Query: 62 HNGSIAFGLTIFNIDQEDLYVYQLNPDNPQQYRYQGQWESMRVLHEPVRIKNGAPAVADL 121 HNGS+AFGLTIFNIDQEDLYVYQLNP++ QY+Y+G WES ++ E + +K G P A+L Sbjct: 325 HNGSVAFGLTIFNIDQEDLYVYQLNPEHSDQYQYRGNWESFNIITEQIPVKGGKPVSAEL 384 Query: 122 SFTRHGPVIYVDQAKHLAYAVRSGWLEPGMSPYFGSIAYMRTRNFKDFKQSMLNWGAPTE 181 F+RHGPVI+ + K A+AVRSGWLEPGMSPYFGSI YMR RNF +FK S+L+WGAPTE Sbjct: 385 VFSRHGPVIFSEPGKSRAFAVRSGWLEPGMSPYFGSIDYMRARNFAEFKHSLLHWGAPTE 444 Query: 182 NQVYADTQGNIGWVPGGLAPKRPNWDGLLPVPGDGRYEWSGFWRGDQLPSSYNPAQGWFA 241 NQVYADT+GNIGWVPGGLAP RPNWDGLLPVPGDGRYEW+GFW GDQLPSSYNP QGWFA Sbjct: 445 NQVYADTKGNIGWVPGGLAPIRPNWDGLLPVPGDGRYEWAGFWSGDQLPSSYNPKQGWFA 504 Query: 242 SANQMNLPAHYPYRERKLGFEWTNNSRFTRISEVLASLPKVSLQDSMRLQNDDLAIPARR 301 SANQMNLP YP+RERKLGFEWTNNSRFTRISEVL+SL KVSL+DSMRLQNDDL+IPARR Sbjct: 505 SANQMNLPDGYPHRERKLGFEWTNNSRFTRISEVLSSLNKVSLEDSMRLQNDDLSIPARR 564 Query: 302 LAALLKPLSSDDANTRAALAMLGQWDYVERSESAQPALYEVWLSRHLGKAFKEAVLPKAA 361 LAALLKPLSS DA T+AAL + WD+VER++SA AL+EVWLSRHLGKAFK AVL AA Sbjct: 565 LAALLKPLSSGDARTQAALNLFAGWDFVERADSAPAALFEVWLSRHLGKAFKAAVLSPAA 624 Query: 362 ADAMGAPDTAVLLDTLENPVPAFGTDAAQARTRRDQVLLASLGAAWAELVKLQGADPAQW 421 A+AMGAPDTAVLLDTLE P FG DA Q +RDQ+LL+SL A+AEL KLQG+D QW Sbjct: 625 AEAMGAPDTAVLLDTLEQPQARFGPDAVQ---KRDQMLLSSLSEAYAELQKLQGSDAGQW 681 Query: 422 QWGKLHHNLMAHPLADVVDEATRKKLNVGPMPKHGGAYSPNQSTYRVNDFRQTNGPSFRV 481 +WGKLHHNLM HPLA VDEATR KLNVGPMP HGGAYSPNQSTYR +DF QTNGPSFRV Sbjct: 682 KWGKLHHNLMEHPLAAAVDEATRAKLNVGPMPTHGGAYSPNQSTYRSSDFLQTNGPSFRV 741 Query: 482 VVDVGRWDNSWAVNAPGQSGDPDSPHYRDLADKWLKGEYFPLLYSRGAIEKVTEHRIVVQ 541 VVDVG WDNS AVN+PGQSGDPDSPHYRDLADKWLKGEYFPLLYSR A+EK T RI +Q Sbjct: 742 VVDVGNWDNSRAVNSPGQSGDPDSPHYRDLADKWLKGEYFPLLYSRAAVEKATVQRIRLQ 801 Query: 542 P 542 P Sbjct: 802 P 802 >ref|YP_998025.1| penicillin amidase [Verminephrobacter eiseniae EF01-2] gb|ABM59007.1| Penicillin amidase [Verminephrobacter eiseniae EF01-2] Length = 782 Score = 811 bits (2096), Expect = 0.0, Method: Composition-based stats. Identities = 400/541 (73%), Positives = 452/541 (83%), Gaps = 17/541 (3%) Query: 2 EGSNNWVIAPSKSATGRAIMANDPHRAYSAPSLRYIAHLSAPGMNVIGAGEPALPGISIG 61 EGSNNWVIAP+KSATGRAIMANDPHRAYSAPSLRYIAHL+APG++VIGAGEPALPGISIG Sbjct: 259 EGSNNWVIAPAKSATGRAIMANDPHRAYSAPSLRYIAHLNAPGLHVIGAGEPALPGISIG 318 Query: 62 HNGSIAFGLTIFNIDQEDLYVYQLNPDNPQQYRYQGQWESMRVLHEPVRIKNGAPAVADL 121 HNG+IAFGLTIF+IDQEDLYVY+LNPDNP +Y+YQG+WE +VLHEP+R+K G P ADL Sbjct: 319 HNGTIAFGLTIFSIDQEDLYVYELNPDNPDEYKYQGKWEPFQVLHEPIRVKGGVPETADL 378 Query: 122 SFTRHGPVIYVDQAKHLAYAVRSGWLEPGMSPYFGSIAYMRTRNFKDFKQSMLNWGAPTE 181 FTRHGPVI+ D+ + A+AVRS WLEPGMSPYFGSI YM ++F FK++++NW APT Sbjct: 379 RFTRHGPVIFADRQR--AFAVRSCWLEPGMSPYFGSIGYMHAKSFNSFKRAIVNWDAPTV 436 Query: 182 NQVYADTQGNIGWVPGGLAPKRPNWDGLLPVPGDGRYEWSGFWRGDQLPSSYNPAQGWFA 241 N VYAD +GNIGW PGGLAPKRPNWDGL+PVPGDGRYEW+GFW GDQLP YNP QGWFA Sbjct: 437 NLVYADVKGNIGWAPGGLAPKRPNWDGLMPVPGDGRYEWAGFWSGDQLPRVYNPRQGWFA 496 Query: 242 SANQMNLPAHYPYRERKLGFEWTNNSRFTRISEVLASLPKVSLQDSMRLQNDDLAIPARR 301 +ANQMNLP YPYRER+LGFEWTN SRF RISEVL+SLPKV+LQD+MRLQNDDLAIPARR Sbjct: 497 TANQMNLPEDYPYRERRLGFEWTNGSRFMRISEVLSSLPKVTLQDAMRLQNDDLAIPARR 556 Query: 302 LAALLKPLSSDDANTRAALAMLGQWDYVERSESAQPALYEVWLSRHLGKAFKEAVLPKAA 361 L ALLK DA T AA+ WDYVER +S Q ALYEVWL+RHLGKAFKEAVL K A Sbjct: 557 LVALLKL----DAGTPAAI--FKGWDYVERVDSPQAALYEVWLARHLGKAFKEAVLSKEA 610 Query: 362 ADAMGAPDTAVLLDTLENPVPAFGTDAAQARTRRDQVLLASLGAAWAELVKLQGADPAQW 421 A AMGAPD AV+L+ LE P G RD++L SL A+AE+VKLQG D +QW Sbjct: 611 AAAMGAPDVAVMLNMLEQPGAVPG---------RDELLRDSLRDAYAEMVKLQGEDASQW 661 Query: 422 QWGKLHHNLMAHPLADVVDEATRKKLNVGPMPKHGGAYSPNQSTYRVNDFRQTNGPSFRV 481 QWGKLHHNLM HPLA VVDE R +LNVGP+PK GGAY PNQSTYR +DFRQTNGPSFRV Sbjct: 662 QWGKLHHNLMEHPLAAVVDEEMRARLNVGPLPKRGGAYGPNQSTYRASDFRQTNGPSFRV 721 Query: 482 VVDVGRWDNSWAVNAPGQSGDPDSPHYRDLADKWLKGEYFPLLYSRGAIEKVTEHRIVVQ 541 VVDVG WD S A+NAPGQSGDP+SPHYRDL ++WL GEYFPLLYSR A+E+ T RIV++ Sbjct: 722 VVDVGHWDGSVAINAPGQSGDPNSPHYRDLTERWLDGEYFPLLYSRKAVEQATVQRIVLK 781 Query: 542 P 542 P Sbjct: 782 P 782 >ref|ZP_07673591.1| penicillin acylase (penicillin amidase) [Ralstonia sp. 5_7_47FAA] gb|EFP68150.1| penicillin acylase (penicillin amidase) [Ralstonia sp. 5_7_47FAA] Length = 809 Score = 790 bits (2039), Expect = 0.0, Method: Composition-based stats. Identities = 387/545 (71%), Positives = 443/545 (81%), Gaps = 3/545 (0%) Query: 1 MEGSNNWVIAPSKSATGRAIMANDPHRAYSAPSLRYIAHLSAPGMNVIGAGEPALPGISI 60 MEGSNNWVIAP KS TGRA+MANDPHRAYSAPSLRYIAH+S P +++IGAGEP+LP +SI Sbjct: 263 MEGSNNWVIAPGKSTTGRAVMANDPHRAYSAPSLRYIAHISTPTLDIIGAGEPSLPAVSI 322 Query: 61 GHNGSIAFGLTIFNIDQEDLYVYQLNPDNPQQYRYQGQWESMRVLHEPVRIKNGAPAVAD 120 GHNG +AFGLTIFNIDQEDLYVY+LNP N +YRY G WE + VLHE VR++ G P D Sbjct: 323 GHNGHVAFGLTIFNIDQEDLYVYELNPANNTEYRYNGGWEPLSVLHETVRVRGGEPRPVD 382 Query: 121 LSFTRHGPVIYVDQAKHLAYAVRSGWLEPGMSPYFGSIAYMRTRNFKDFKQSMLNWGAPT 180 L+FTRHGPVIY+D KH AYAVRS WL PGMSPYFGS+ YMR R F FKQSM+NWGAPT Sbjct: 383 LTFTRHGPVIYIDAEKHRAYAVRSAWLSPGMSPYFGSVGYMRARTFAQFKQSMMNWGAPT 442 Query: 181 ENQVYADTQGNIGWVPGGLAPKRPNWDGLLPVPGDGRYEWSGFWRGDQLPSSYNPAQGWF 240 ENQVYADT+GNIGWVPGGLAP RPNWDGLLPVPGDGRYEW+GFWRGDQLPSSYNP G+F Sbjct: 443 ENQVYADTKGNIGWVPGGLAPIRPNWDGLLPVPGDGRYEWAGFWRGDQLPSSYNPKSGYF 502 Query: 241 ASANQMNLPAHYPYRERKLGFEWTNNSRFTRISEVLASLPKVSLQDSMRLQNDDLAIPAR 300 S+N+MNLP YPYRERKLGFEWTN SR RI EVLA L KVS++DSMRLQND ++IPAR Sbjct: 503 TSSNEMNLPPDYPYRERKLGFEWTNGSRHARIDEVLARLDKVSIEDSMRLQNDMVSIPAR 562 Query: 301 RLAALLKPLSSDDANTRAALAMLGQWDYVERSESAQPALYEVWLSRHLGKAFKEAVLPKA 360 RL ALL LSS+DA+T+AAL + W+ ++S Q AL+EVW + HLG+AFK AVL KA Sbjct: 563 RLMALLAHLSSNDADTQAALNLFKGWNATMLADSPQAALHEVWFTHHLGRAFKNAVLSKA 622 Query: 361 AADAMGAPDTAVLLDTLENPVPA---FGTDAAQARTRRDQVLLASLGAAWAELVKLQGAD 417 +A+AMGAPDT V+LDTLE+P + FG D QA +R+ VLL SL AW ++VK QG + Sbjct: 623 SAEAMGAPDTDVMLDTLEHPQGSERKFGEDPQQAAAKRNAVLLDSLKNAWTDMVKRQGPN 682 Query: 418 PAQWQWGKLHHNLMAHPLADVVDEATRKKLNVGPMPKHGGAYSPNQSTYRVNDFRQTNGP 477 P W WG+LHHNL AHP A +VDEATR KLNVGP P+ G Y+PNQSTYR +DF QTNGP Sbjct: 683 PQAWNWGQLHHNLNAHPFAAIVDEATRAKLNVGPTPEGGSPYTPNQSTYRASDFLQTNGP 742 Query: 478 SFRVVVDVGRWDNSWAVNAPGQSGDPDSPHYRDLADKWLKGEYFPLLYSRGAIEKVTEHR 537 SFR VVDVG WDNS A+N PGQSG+PDSPHYRDLA WLKGEYFPLLYSR A+E TE R Sbjct: 743 SFRTVVDVGNWDNSRAINLPGQSGNPDSPHYRDLAPLWLKGEYFPLLYSRKAVEAATESR 802 Query: 538 IVVQP 542 + + P Sbjct: 803 VHLVP 807 >ref|YP_001892723.1| peptidase S45 penicillin amidase [Ralstonia pickettii 12J] ref|YP_002984224.1| peptidase S45 penicillin amidase [Ralstonia pickettii 12D] gb|ACD29296.1| peptidase S45 penicillin amidase [Ralstonia pickettii 12J] gb|ACS65552.1| peptidase S45 penicillin amidase [Ralstonia pickettii 12D] Length = 809 Score = 788 bits (2035), Expect = 0.0, Method: Composition-based stats. Identities = 385/545 (70%), Positives = 442/545 (81%), Gaps = 3/545 (0%) Query: 1 MEGSNNWVIAPSKSATGRAIMANDPHRAYSAPSLRYIAHLSAPGMNVIGAGEPALPGISI 60 MEGSNNWVIAP KS TGRA+MANDPHRAYSAPSLRYIAH+S P +++IGAGEP+LP +SI Sbjct: 263 MEGSNNWVIAPGKSTTGRAVMANDPHRAYSAPSLRYIAHVSTPTLDIIGAGEPSLPAVSI 322 Query: 61 GHNGSIAFGLTIFNIDQEDLYVYQLNPDNPQQYRYQGQWESMRVLHEPVRIKNGAPAVAD 120 GHNG +AFGLTIFNIDQEDLYVY+LNP N +YRY G WE VLHE V+++ G D Sbjct: 323 GHNGHVAFGLTIFNIDQEDLYVYELNPANNGEYRYNGGWEPFIVLHETVKVRGGESRPVD 382 Query: 121 LSFTRHGPVIYVDQAKHLAYAVRSGWLEPGMSPYFGSIAYMRTRNFKDFKQSMLNWGAPT 180 L+FTRHGPVIY+D KH AYAVRS WL PGMSPYFGS+ YMR R F F+QSM+NWGAPT Sbjct: 383 LTFTRHGPVIYIDAEKHRAYAVRSAWLSPGMSPYFGSVGYMRARTFAQFRQSMMNWGAPT 442 Query: 181 ENQVYADTQGNIGWVPGGLAPKRPNWDGLLPVPGDGRYEWSGFWRGDQLPSSYNPAQGWF 240 ENQVYADT+GNIGWVPGGLAP RPNWDGLLPVPGDGRYEW+GFWRGDQLPSSYNP G+F Sbjct: 443 ENQVYADTKGNIGWVPGGLAPIRPNWDGLLPVPGDGRYEWAGFWRGDQLPSSYNPKSGYF 502 Query: 241 ASANQMNLPAHYPYRERKLGFEWTNNSRFTRISEVLASLPKVSLQDSMRLQNDDLAIPAR 300 S+N+MNLPA YPYRERKLGFEWTN SR RI EVL+ L KVS++DSMRLQND ++IPAR Sbjct: 503 TSSNEMNLPADYPYRERKLGFEWTNGSRHVRIDEVLSKLDKVSIEDSMRLQNDMVSIPAR 562 Query: 301 RLAALLKPLSSDDANTRAALAMLGQWDYVERSESAQPALYEVWLSRHLGKAFKEAVLPKA 360 RL ALL PLSSDDA+T+AAL + W+ ++S Q AL+EVW + HLG+AFK AVL KA Sbjct: 563 RLTALLAPLSSDDADTQAALNLFKGWNATMLADSPQAALHEVWFTHHLGRAFKNAVLSKA 622 Query: 361 AADAMGAPDTAVLLDTLENPVPA---FGTDAAQARTRRDQVLLASLGAAWAELVKLQGAD 417 +A+AM APDT V+LDTLE+P + FG D QA +RD VLL SL AW ++VK QG + Sbjct: 623 SAEAMAAPDTDVMLDTLEHPQGSDRKFGEDPQQAAAKRDAVLLDSLKNAWTDMVKRQGPN 682 Query: 418 PAQWQWGKLHHNLMAHPLADVVDEATRKKLNVGPMPKHGGAYSPNQSTYRVNDFRQTNGP 477 P W WG+LHHNL AHP +VDEATR KLNVGP P+ G Y+PNQSTYR +DF QTNGP Sbjct: 683 PQAWNWGQLHHNLNAHPFTAIVDEATRAKLNVGPTPEGGSPYTPNQSTYRASDFLQTNGP 742 Query: 478 SFRVVVDVGRWDNSWAVNAPGQSGDPDSPHYRDLADKWLKGEYFPLLYSRGAIEKVTEHR 537 SFR VVDVG+WDNS AVN PGQSG+PDSPHYRDLA WLKG+YFPLLYSR A+E TE R Sbjct: 743 SFRTVVDVGKWDNSRAVNLPGQSGNPDSPHYRDLAPLWLKGKYFPLLYSRKAVEAATESR 802 Query: 538 IVVQP 542 + + P Sbjct: 803 VHLVP 807 >emb|CCA81837.1| putative penicillin G amidase precursor; peptidase S45 family [blood disease bacterium R229] Length = 811 Score = 776 bits (2003), Expect = 0.0, Method: Composition-based stats. Identities = 385/545 (70%), Positives = 438/545 (80%), Gaps = 3/545 (0%) Query: 1 MEGSNNWVIAPSKSATGRAIMANDPHRAYSAPSLRYIAHLSAPGMNVIGAGEPALPGISI 60 MEGSNNWVIAP KSATGRA+MANDPHRAYSAP LRYI H+S P +++IGAGEP+LP +SI Sbjct: 265 MEGSNNWVIAPGKSATGRAVMANDPHRAYSAPGLRYIVHISTPTLDIIGAGEPSLPAVSI 324 Query: 61 GHNGSIAFGLTIFNIDQEDLYVYQLNPDNPQQYRYQGQWESMRVLHEPVRIKNGAPAVAD 120 GHNG IAFGLTIFNIDQEDLYVY+L+P NP YRY+ WE VLHE V+++ + Sbjct: 325 GHNGHIAFGLTIFNIDQEDLYVYELDPANPNAYRYKDGWEPFTVLHETVKVRGADARPVE 384 Query: 121 LSFTRHGPVIYVDQAKHLAYAVRSGWLEPGMSPYFGSIAYMRTRNFKDFKQSMLNWGAPT 180 L+FTRHGPVIYVD +H AYAVRS WL+PGMSPYFGS+ Y+R R+F FK++MLNWGAPT Sbjct: 385 LTFTRHGPVIYVDADRHRAYAVRSAWLQPGMSPYFGSVGYVRARSFAQFKRAMLNWGAPT 444 Query: 181 ENQVYADTQGNIGWVPGGLAPKRPNWDGLLPVPGDGRYEWSGFWRGDQLPSSYNPAQGWF 240 ENQVYADT+GNIGWVPGGLAP RPNWDGLLPVPGDGRYEW+GFWRGDQLPS+YNP G+F Sbjct: 445 ENQVYADTRGNIGWVPGGLAPIRPNWDGLLPVPGDGRYEWAGFWRGDQLPSAYNPKSGYF 504 Query: 241 ASANQMNLPAHYPYRERKLGFEWTNNSRFTRISEVLASLPKVSLQDSMRLQNDDLAIPAR 300 S+N+MNLPA YPYRERKLGFEWTN SR RI EVLA L VSL+DSMRLQND ++IPAR Sbjct: 505 TSSNEMNLPADYPYRERKLGFEWTNGSRHARIDEVLARLDSVSLEDSMRLQNDMVSIPAR 564 Query: 301 RLAALLKPLSSDDANTRAALAMLGQWDYVERSESAQPALYEVWLSRHLGKAFKEAVLPKA 360 RL ALL PLSS DA T+AAL + W ++S Q AL+EVWL+ HLG+AFK AVL KA Sbjct: 565 RLMALLTPLSSSDAGTQAALDLFKGWGATMLADSPQAALHEVWLTHHLGRAFKHAVLSKA 624 Query: 361 AADAMGAPDTAVLLDTLENPVPA---FGTDAAQARTRRDQVLLASLGAAWAELVKLQGAD 417 AADAMGAPD AV+LD LE+P A FG D QA +RD VLL+SL AWA++VK QG D Sbjct: 625 AADAMGAPDPAVMLDVLEHPQGAERRFGEDPQQAAAKRDAVLLSSLRDAWADMVKRQGPD 684 Query: 418 PAQWQWGKLHHNLMAHPLADVVDEATRKKLNVGPMPKHGGAYSPNQSTYRVNDFRQTNGP 477 P W WG LHHNL AHP A +VD+ R KLNVGP P+ G AY+PNQSTYR +DF QTNGP Sbjct: 685 PRAWNWGALHHNLNAHPFAAIVDDTMRAKLNVGPTPEGGSAYTPNQSTYRASDFLQTNGP 744 Query: 478 SFRVVVDVGRWDNSWAVNAPGQSGDPDSPHYRDLADKWLKGEYFPLLYSRGAIEKVTEHR 537 SFR V+DVG WDNS AVN PGQSGDPDSPHYRDLA WL G+YFPLLYSR A+E TE R Sbjct: 745 SFRTVIDVGNWDNSRAVNLPGQSGDPDSPHYRDLAPMWLTGQYFPLLYSRRAVESATEMR 804 Query: 538 IVVQP 542 I + P Sbjct: 805 IRLVP 809