BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2528.p (247 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_998025.1| penicillin amidase [Verminephrobacter eiseniae ... 335 4e-90 ref|ZP_08871773.1| penicillin amidase, partial [Verminephrobacte... 331 5e-89 ref|YP_004751856.1| Penicillin amidase [Collimonas fungivorans T... 327 8e-88 ref|ZP_07673591.1| penicillin acylase (penicillin amidase) [Rals... 306 3e-81 ref|YP_001892723.1| peptidase S45 penicillin amidase [Ralstonia ... 304 9e-81 >ref|YP_998025.1| penicillin amidase [Verminephrobacter eiseniae EF01-2] gb|ABM59007.1| Penicillin amidase [Verminephrobacter eiseniae EF01-2] Length = 782 Score = 335 bits (858), Expect = 4e-90, Method: Composition-based stats. Identities = 160/209 (76%), Positives = 183/209 (87%) Query: 17 ASLPLAASAQPASTEKIAIPGLQQRAEILIDRWGVPHIYAASERDAFFMQGFNAARDRLF 76 A L +A + A+T+ + +PGLQ++AEILID+WGVPHIYAAS+ DAFF+QGFNAARDRLF Sbjct: 10 ALLACSAISSHAATQTLHVPGLQKKAEILIDKWGVPHIYAASQTDAFFVQGFNAARDRLF 69 Query: 77 QIDLWRRRGLGQLSEVFGPAYLPQDRATRLFLYRGDMRREWAAYGPGAESVAQAFVEGIN 136 QIDLWRRRGLGQLSEVFGPAY+ QD+ATRLFLYRGDM+REW +YG +E +A AFV GIN Sbjct: 70 QIDLWRRRGLGQLSEVFGPAYIAQDQATRLFLYRGDMQREWDSYGKQSEQIATAFVAGIN 129 Query: 137 AYIDYVSLHQEKLPYEFRQLGYLPAKWQAEDVVRIRSHGLTRNLTSEVARANVACKADLK 196 AYID V+ H E+LPYEF+QL Y PAKWQAEDVVRIRSHGLTRNL SEVARANV CKADLK Sbjct: 130 AYIDLVTAHPERLPYEFKQLAYQPAKWQAEDVVRIRSHGLTRNLNSEVARANVVCKADLK 189 Query: 197 SDEIRSGLTPKWEAIMPQGLDPCLPNDLL 225 SDEIR GL PKW+A+MP+GLDPCLP DLL Sbjct: 190 SDEIRFGLQPKWQAVMPEGLDPCLPKDLL 218 >ref|ZP_08871773.1| penicillin amidase, partial [Verminephrobacter sp. At4] Length = 432 Score = 331 bits (849), Expect = 5e-89, Method: Composition-based stats. Identities = 159/215 (73%), Positives = 186/215 (86%), Gaps = 2/215 (0%) Query: 11 ARLSMAASLPLAASAQPASTEKIAIPGLQQRAEILIDRWGVPHIYAASERDAFFMQGFNA 70 A LS+ +PLA +A +TE + +PGLQQ+AEILID WGVPHIYAAS+ DAFF+QGFNA Sbjct: 16 AALSLCLLMPLAHAA--GTTETLRVPGLQQKAEILIDEWGVPHIYAASQTDAFFVQGFNA 73 Query: 71 ARDRLFQIDLWRRRGLGQLSEVFGPAYLPQDRATRLFLYRGDMRREWAAYGPGAESVAQA 130 ARDRLFQIDLWRRRGLGQL++VFGPAY+ QD+ATRLFLYRGDM+REW AYG AE VA A Sbjct: 74 ARDRLFQIDLWRRRGLGQLAQVFGPAYVAQDQATRLFLYRGDMQREWNAYGKQAEKVATA 133 Query: 131 FVEGINAYIDYVSLHQEKLPYEFRQLGYLPAKWQAEDVVRIRSHGLTRNLTSEVARANVA 190 FV GINAY+D+V+ H E+LPYEF+QL YLPA+WQA+DVVRIRSHGLTRNL SEVARA+V Sbjct: 134 FVAGINAYVDFVTAHPERLPYEFKQLAYLPARWQAQDVVRIRSHGLTRNLNSEVARAHVV 193 Query: 191 CKADLKSDEIRSGLTPKWEAIMPQGLDPCLPNDLL 225 CKA+L+SD +R GL PKWEA +P+GLDPCLP DLL Sbjct: 194 CKANLESDTVRLGLKPKWEATLPEGLDPCLPEDLL 228 >ref|YP_004751856.1| Penicillin amidase [Collimonas fungivorans Ter331] gb|AEK61033.1| Penicillin amidase [Collimonas fungivorans Ter331] Length = 804 Score = 327 bits (839), Expect = 8e-88, Method: Composition-based stats. Identities = 161/219 (73%), Positives = 182/219 (83%), Gaps = 2/219 (0%) Query: 7 AVLLARLSMAASLPLAASAQPASTEKIAIPGLQQRAEILIDRWGVPHIYAASERDAFFMQ 66 A LL + AS A P +T KIA GL++ AEIL+D+WGVPHIYAA++ DAFF+Q Sbjct: 11 AALLVSFAATASQAKDTPAAPVTTYKIA--GLKKPAEILVDQWGVPHIYAANQDDAFFVQ 68 Query: 67 GFNAARDRLFQIDLWRRRGLGQLSEVFGPAYLPQDRATRLFLYRGDMRREWAAYGPGAES 126 GFNAARDRLFQIDLWRRRGLG L++VFGPAY+ QDRA RLFLYRGDM+REW +YGP A+ Sbjct: 69 GFNAARDRLFQIDLWRRRGLGLLAQVFGPAYVQQDRAARLFLYRGDMQREWQSYGPHAQQ 128 Query: 127 VAQAFVEGINAYIDYVSLHQEKLPYEFRQLGYLPAKWQAEDVVRIRSHGLTRNLTSEVAR 186 VA FV GINAY+DYV+ H EKLP+EFR+LGY PA+WQA DVVRIRSHGLTRNL SEVAR Sbjct: 129 VANTFVAGINAYVDYVNAHPEKLPFEFRRLGYAPARWQAADVVRIRSHGLTRNLDSEVAR 188 Query: 187 ANVACKADLKSDEIRSGLTPKWEAIMPQGLDPCLPNDLL 225 ANVACKADLKSDEIR GLTPKWE MP+GLDPCLP DLL Sbjct: 189 ANVACKADLKSDEIRFGLTPKWETTMPEGLDPCLPKDLL 227 >ref|ZP_07673591.1| penicillin acylase (penicillin amidase) [Ralstonia sp. 5_7_47FAA] gb|EFP68150.1| penicillin acylase (penicillin amidase) [Ralstonia sp. 5_7_47FAA] Length = 809 Score = 306 bits (783), Expect = 3e-81, Method: Composition-based stats. Identities = 148/226 (65%), Positives = 175/226 (77%), Gaps = 1/226 (0%) Query: 1 MPRLNRAVLLARLSMAASLPLAASAQPAS-TEKIAIPGLQQRAEILIDRWGVPHIYAASE 59 MP + +LL+ +++ S A P S T+ IA+PGL + A+ILIDRWGVPHIYA SE Sbjct: 1 MPIRSLPILLSAVALGFSAQAVAKDAPTSATQTIAVPGLSKPADILIDRWGVPHIYAKSE 60 Query: 60 RDAFFMQGFNAARDRLFQIDLWRRRGLGQLSEVFGPAYLPQDRATRLFLYRGDMRREWAA 119 D FF QGFNAARDRLFQIDLWRRRGLGQLSEVFGP Y+ QDRATRLFLYRGDM+ EW Sbjct: 61 DDGFFAQGFNAARDRLFQIDLWRRRGLGQLSEVFGPTYVEQDRATRLFLYRGDMQVEWNH 120 Query: 120 YGPGAESVAQAFVEGINAYIDYVSLHQEKLPYEFRQLGYLPAKWQAEDVVRIRSHGLTRN 179 Y P A+ +A FV GINAY+D+++ H +++P+EFR+L Y PA W EDVVRIRSHGLTRN Sbjct: 121 YSPDAQRIATRFVAGINAYVDWLAKHPDQMPFEFRKLNYAPAHWAPEDVVRIRSHGLTRN 180 Query: 180 LTSEVARANVACKADLKSDEIRSGLTPKWEAIMPQGLDPCLPNDLL 225 L SEVARANVACKA+L D IR GL P W+ +P+GLDPCLP DLL Sbjct: 181 LQSEVARANVACKANLADDTIRFGLQPAWQTQLPEGLDPCLPKDLL 226 >ref|YP_001892723.1| peptidase S45 penicillin amidase [Ralstonia pickettii 12J] ref|YP_002984224.1| peptidase S45 penicillin amidase [Ralstonia pickettii 12D] gb|ACD29296.1| peptidase S45 penicillin amidase [Ralstonia pickettii 12J] gb|ACS65552.1| peptidase S45 penicillin amidase [Ralstonia pickettii 12D] Length = 809 Score = 304 bits (778), Expect = 9e-81, Method: Composition-based stats. Identities = 147/226 (65%), Positives = 177/226 (78%), Gaps = 1/226 (0%) Query: 1 MPRLNRAVLLARLSMAASL-PLAASAQPASTEKIAIPGLQQRAEILIDRWGVPHIYAASE 59 MP + ++LL+ +++ S +A A A+ + IA+PGL + A+ILIDRWGVPHIYA SE Sbjct: 1 MPIRSLSILLSAVALGFSAQAVAKDAHTAAAQTIAVPGLSKPADILIDRWGVPHIYARSE 60 Query: 60 RDAFFMQGFNAARDRLFQIDLWRRRGLGQLSEVFGPAYLPQDRATRLFLYRGDMRREWAA 119 D FF QGFNAARDRLFQIDLWRRRGLGQLSEVFGPAY+ QDRATRLFLYRGDM+ EW Sbjct: 61 DDGFFAQGFNAARDRLFQIDLWRRRGLGQLSEVFGPAYVEQDRATRLFLYRGDMQVEWNH 120 Query: 120 YGPGAESVAQAFVEGINAYIDYVSLHQEKLPYEFRQLGYLPAKWQAEDVVRIRSHGLTRN 179 Y P A+ +A FV GINAY+D+++ H E++P+EFR+L Y PA W EDVVRIRSHGLTRN Sbjct: 121 YSPDAQRIATRFVAGINAYVDWLAKHPEQMPFEFRKLNYAPAHWAPEDVVRIRSHGLTRN 180 Query: 180 LTSEVARANVACKADLKSDEIRSGLTPKWEAIMPQGLDPCLPNDLL 225 L SEVARANVACKA+L D +R L P W+ +P+GLDPCLP DLL Sbjct: 181 LQSEVARANVACKANLSDDTVRFRLQPAWQTQLPEGLDPCLPKDLL 226