BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2524.p (1005 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774262.1| diguanylate cyclase/phosphodiesterase A prot... 1761 0.0 ref|YP_003279514.1| diguanylate cyclase/phosphodiesterase [Comam... 909 0.0 ref|ZP_07046685.1| diguanylate cyclase/phosphodiesterase [Comamo... 905 0.0 ref|ZP_03541929.1| diguanylate cyclase/phosphodiesterase with PA... 894 0.0 ref|ZP_06685654.1| diguanylate cyclase/phosphodiesterase with PA... 886 0.0 >ref|YP_003774262.1| diguanylate cyclase/phosphodiesterase A protein [Herbaspirillum seropedicae SmR1] gb|ADJ62354.1| diguanylate cyclase/phosphodiesterase A protein [Herbaspirillum seropedicae SmR1] Length = 1002 Score = 1761 bits (4562), Expect = 0.0, Method: Composition-based stats. Identities = 855/1003 (85%), Positives = 918/1003 (91%), Gaps = 9/1003 (0%) Query: 1 MGSQESFVGQFDHPSQPQGDHQRLREEEREALLGALWRSMAILEFAADGSILDANDKFLG 60 MGSQ+SF+G+ DH QRL ER+ALL ALWRSMAILEF+ADG++L+AN+ FL Sbjct: 1 MGSQDSFMGEVDH--------QRLSGSERDALLAALWRSMAILEFSADGTVLEANENFLA 52 Query: 61 MLGYQRHEVVGQHHRMLCDPLSVETEDYQRFWQHLRQGEVIAGTLQRRRIDGTIVWLEAL 120 MLGY+R +VVGQHHRMLCDP V+T+ YQ+FW+HLR+GE+IAGTLQRR + G VWLEAL Sbjct: 53 MLGYRRADVVGQHHRMLCDPAYVQTDAYQQFWEHLRKGELIAGTLQRRHVGGATVWLEAL 112 Query: 121 CSPILDGSGQLRKVISVAADISSHIDDSHHSNNVLSALNRSLGVVELSPQGMILAANRNY 180 CSPILD GQL KVISVAADIS+HIDDS HSN++LSALNRSLGVVELSP+G+ILAAN NY Sbjct: 113 CSPILDAQGQLLKVISVAADISAHIDDSRHSNSILSALNRSLGVVELSPEGVILAANGNY 172 Query: 181 LKTMEYLADDLIGQPHSVLCPPGYADSEEYAIFWRDVCSGRFFSGRFPRRTRSGRTIWWE 240 LKTMEYLA+DL+GQPHS+LC YA SEEY FWRDV GRFFSGRFPRRTRSGRTIWWE Sbjct: 173 LKTMEYLAEDLLGQPHSILCEADYAASEEYTCFWRDVSLGRFFSGRFPRRTRSGRTIWWE 232 Query: 241 ATYSPLLDPQGKAERVICIGNDVTYRVTREKSDREHLHLLSLGINETDNAVLITDEQNRI 300 ATYSPLLD GKAERVICIG DVTYRVTREKSDREHLHLLSLGINETDNAVLITD+QNRI Sbjct: 233 ATYSPLLDAHGKAERVICIGTDVTYRVTREKSDREHLHLLSLGINETDNAVLITDDQNRI 292 Query: 301 VFHNTGFTRMLGFQSQQTIGKLPRELFASHPTFVKSIERCHGELLQGHSFHTEELIHDLH 360 VFHNTGFTRMLGF+SQQ IG+LP ELFAS PTFVKS+ERCHGEL+QG S+HTEELIHD+ Sbjct: 293 VFHNTGFTRMLGFESQQVIGRLPGELFASRPTFVKSLERCHGELVQGRSYHTEELIHDMQ 352 Query: 361 EQPVWISAVVNPILDVQGKLINAVCVMTDITNTKVHEVLQYRVLNAMARDSSLAEQMNLL 420 EQPVWISAVVNPILD GKLI+AVCVMTDITNTKVHEVLQYRVLNAMARDSSL+EQMNLL Sbjct: 353 EQPVWISAVVNPILDGAGKLIHAVCVMTDITNTKVHEVLQYRVLNAMARDSSLSEQMNLL 412 Query: 421 CEEVERVAPEVIASVLRVDEQGRLHTLAAPSLPEAYTRAIEGIQIGPLVGSCGSAAYTGE 480 CEEVERVAPEVIASVLRVDE G L TLAAPSLPEAY++AI+G+ +GP+VG CGSAAYTG+ Sbjct: 413 CEEVERVAPEVIASVLRVDEHGCLRTLAAPSLPEAYSKAIDGLHVGPMVGCCGSAAYTGQ 472 Query: 481 SVVSEEIATDPHWADYKEIALSHGLQACWSTPIKSADGKVIGTFGFYYRQPRAPSAFHHL 540 +V+SE+I TDPHW DYK +AL HGL+ACWSTPIKSA G+VIGTFGFYYR P PSAFHHL Sbjct: 473 TVISEDIQTDPHWNDYKHLALPHGLRACWSTPIKSASGQVIGTFGFYYRHPGQPSAFHHL 532 Query: 541 LVDAGLHLCALAIEREASRARIHQLAFYDSLTGLPNRSLLLVQAGQAIAAAERNRSMVAV 600 LV+AGLHLCALAIERE +RARIHQLAFYDSLTGLPNRSLLLVQAGQAIAAAERN S VAV Sbjct: 533 LVEAGLHLCALAIEREDARARIHQLAFYDSLTGLPNRSLLLVQAGQAIAAAERNCSTVAV 592 Query: 601 MFIDLDRFKQVNDSLGHQAGDELLRTVATRLQSQARRSDIVGRLSGDEFVVVLTQYDTDH 660 MFIDLDRFKQVNDSLGHQAGDELLRTVATRLQSQARRSDIVGRLSGDEFVVVLTQYDT H Sbjct: 593 MFIDLDRFKQVNDSLGHQAGDELLRTVATRLQSQARRSDIVGRLSGDEFVVVLTQYDTQH 652 Query: 661 LTEVVKRIQERLAMPCHINGTPLNPSASIGVAIFPDNGHDIETLLHRADIAMYQAKAVNR 720 LTEVVKRIQERLAMPCHINGTPLNPSASIGVAIFPDNG+DIETLLHRADIAMYQAKAVNR Sbjct: 653 LTEVVKRIQERLAMPCHINGTPLNPSASIGVAIFPDNGNDIETLLHRADIAMYQAKAVNR 712 Query: 721 GRFSFFSEEMNSLAQERLVLEGALRDALRGGSFELHFQPQINLHDLSLHGVEALARWYDP 780 GRFSFFSEEMNS+AQERL LE ALRDALRGG EL+FQPQI LHD SLHGVEALARW+ P Sbjct: 713 GRFSFFSEEMNSMAQERLALESALRDALRGGDLELNFQPQIKLHDQSLHGVEALARWHHP 772 Query: 781 QLGQISPGRFIPIAEECGLIGELGKWALEEACFQLADWRRRGIQVPSVSVNLSATNFHDL 840 QLGQISPGRFIPIAEECGLIGELGKWALEEACFQL+DWRRRGIQVPS+SVNLSATNFHDL Sbjct: 773 QLGQISPGRFIPIAEECGLIGELGKWALEEACFQLSDWRRRGIQVPSISVNLSATNFHDL 832 Query: 841 DLPQFLTGLLRRYSLAPTDLTLEITETVLMDTNPSTNQTIAAVHALGIPLAMDDFGTGYS 900 DLPQF+TGLLRRY+L P+DLTLEITETVLMDTNPST+QTIAAVHALGIPLAMDDFGTGYS Sbjct: 833 DLPQFVTGLLRRYALEPSDLTLEITETVLMDTNPSTSQTIAAVHALGIPLAMDDFGTGYS 892 Query: 901 SLGYLRKLPVSELKLDQSFVRDMAKDEAVSALTNAVIRIGESLKLTVVAEGVEDADQYRL 960 SLGYLRKLPVSELKLDQSFVRDMAKD AVSALTNAVIRIGESLKLTVVAEGVEDA+QY L Sbjct: 893 SLGYLRKLPVSELKLDQSFVRDMAKDPAVSALTNAVIRIGESLKLTVVAEGVEDAEQYAL 952 Query: 961 LISQGCDVAQGYYFSRPMSADQLESWVRDYRGENAAPPGATGP 1003 LISQGCDVAQGY FSRPMSAD LE+WVRD +P G P Sbjct: 953 LISQGCDVAQGYLFSRPMSADALENWVRDLHA-GGSPDGRWAP 994 >ref|YP_003279514.1| diguanylate cyclase/phosphodiesterase [Comamonas testosteroni CNB-2] gb|ACY34218.1| diguanylate cyclase/phosphodiesterase [Comamonas testosteroni CNB-2] Length = 868 Score = 909 bits (2350), Expect = 0.0, Method: Composition-based stats. Identities = 461/851 (54%), Positives = 591/851 (69%), Gaps = 7/851 (0%) Query: 155 LSALNRSLGVVELSPQGMILAANRNYLKTMEYLADDLIGQPHSVLCPPGYADSEEYAIFW 214 L AL+R + + E G + AN NYL+ M + + +G+PH C ADS Y W Sbjct: 18 LQALDRVMAMAEFGLDGALHRANDNYLQLMGFTREQSLGRPHRSFCSARLADSARYREIW 77 Query: 215 RDVCSGRFFSGRFPRRTRSGRTIWWEATYSPLLDPQGKAERVICIGNDVTYRVTREKSDR 274 +C+G +SG R G + W EATYSP++D QG+ ++ I DVT R RE++ + Sbjct: 78 THLCAGNAYSGVVERLRSDGSSCWLEATYSPVMDAQGRVMHILKIATDVTQRRAREQAQQ 137 Query: 275 EHLHLLSLGINETDNAVLITDEQNRIVFHNTGFTRMLGFQSQQTIGKLPRELFASHPTFV 334 EHL LSL + +D AV+I+D +RIV N GFTRM G+++++ G++P L A + Sbjct: 138 EHLRRLSLVADASDTAVVISDGSSRIVHVNGGFTRMFGWRTEEVAGRMPIALLAPQAS-E 196 Query: 335 KSIERCHGELLQGHSFHTEELIHDLHEQPVWISAVVNPILDVQGKLINAVCVMTDITNTK 394 ++ EL G EE++ H Q W + NPILD G+ V ++TDIT +K Sbjct: 197 AFVDHYRAELRAGQPVGREEIVVGKHGQRYWAKVISNPILDADGRWQYTVSMLTDITRSK 256 Query: 395 VHEVLQYRVLNAMARDSSLAEQMNLLCEEVERVAPEVIASVLRVDEQGRLHTLAAPSLPE 454 +HE LQ+RVL AMAR+ L E + ++C+EVER+APEV AS+L VD QG LH LA PSLP Sbjct: 257 MHEALQHRVLEAMARERPLVEVLEMVCQEVERIAPEVTASILEVDAQGLLHPLAGPSLPH 316 Query: 455 AYTRAIEGIQIGPLVGSCGSAAYTGESVVSEEIATDPHWADYKEIALSHGLQACWSTPIK 514 +Y+ ++G+ IGP VGSCG+AA+ V+ ++IA DP W D+K + L G +ACWSTPI Sbjct: 317 SYSALLDGVAIGPNVGSCGTAAWRNAPVLVDDIAHDPLWTDFKHLILPLGFKACWSTPIC 376 Query: 515 SADGKVIGTFGFYYRQPRA--PSAFHHLLVDAGLHLCALAIEREASRARIHQLAFYDSLT 572 ++ GK IGTF FYYR+ SAFH LVDA +HLCALA+ERE SRARI QLAFYD LT Sbjct: 377 NSGGKPIGTFAFYYREQLTGDASAFHQQLVDACIHLCALALERELSRARIRQLAFYDGLT 436 Query: 573 GLPNRSLLLVQAGQAIAAAERNRSMVAVMFIDLDRFKQVNDSLGHQAGDELLRTVATRLQ 632 GLPNRSLL +A QAIA+A N +AV+FIDLDRFKQVNDSLGH GDELLR VATRLQ Sbjct: 437 GLPNRSLLQAKADQAIASAAGNDEQLAVLFIDLDRFKQVNDSLGHPVGDELLRNVATRLQ 496 Query: 633 SQARRSDIVGRLSGDEFVVVLTQYDTDHLTEVVKRIQERLAMPCHINGTPLNPSASIGVA 692 R SDI GRLSGDEFV VL Q D +H+ ++R+QE LA P +I T L+ SAS+G+A Sbjct: 497 RVLRSSDIAGRLSGDEFVAVLPQCDAEHVANTIERLQELLAEPLNIADTSLSISASVGIA 556 Query: 693 IFPDNGHDIETLLHRADIAMYQAKAVNRGRFSFFSEEMNSLAQERLVLEGALRDALRGGS 752 +FP +G D+ETL+HRAD+AMYQAK+ RGRF FFS EMN LAQERL LE ALR AL+ G Sbjct: 557 MFPMDGRDMETLVHRADMAMYQAKSKGRGRFCFFSSEMNRLAQERLALETALRKALKSGG 616 Query: 753 FELHFQPQINLHDLSLHGVEALARWYDPQLGQISPGRFIPIAEECGLIGELGKWALEEAC 812 LH+QPQI + L+GVEALARW +LG+ISP RFIP+AEECGLI +LG WALEEAC Sbjct: 617 LRLHYQPQIEMATGRLYGVEALARWTHAELGEISPARFIPLAEECGLIADLGSWALEEAC 676 Query: 813 FQLADWRRRGIQVPSVSVNLSATNFHDLDLPQFLTGLLRRYSLAPTDLTLEITETVLMDT 872 QL+ WR +G++VP+VSVNLS ++FH+LDLP+ + L R L P DLTLE+TE++L+DT Sbjct: 677 RQLSQWRAKGMEVPAVSVNLSPSSFHNLDLPRMIADTLDRNDLVPQDLTLELTESILLDT 736 Query: 873 NPSTNQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQSFVRDMAKDEAVSAL 932 NPST +TI VHA G+ L+MDDFGTGYSSL YLR+LPVSELKLD+SFV D+ DEA AL Sbjct: 737 NPSTMKTINEVHAHGVRLSMDDFGTGYSSLSYLRRLPVSELKLDRSFVADLEHDEAARAL 796 Query: 933 TNAVIRIGESLKLTVVAEGVEDADQYRLLISQGCDVAQGYYFSRPMSADQLESWVRDYRG 992 ++A++ IG+SL LTVVAEGVE Q +L QG VAQG+ F+RP++ + E W+ Sbjct: 797 SSAILGIGKSLHLTVVAEGVETPTQSVMLCEQGYPVAQGFLFARPLAPKEFEHWL----A 852 Query: 993 ENAAPPGATGP 1003 N A P +T P Sbjct: 853 ANPASPVSTAP 863 Score = 102 bits (255), Expect = 3e-19, Method: Composition-based stats. Identities = 67/254 (26%), Positives = 122/254 (48%), Gaps = 10/254 (3%) Query: 18 QGDHQRLREEEREALLGALWRSMAILEFAADGSILDANDKFLGMLGYQRHEVVGQHHRML 77 +GD + E + A L AL R MA+ EF DG++ AND +L ++G+ R + +G+ HR Sbjct: 3 EGDIGAMPPELQLAQLQALDRVMAMAEFGLDGALHRANDNYLQLMGFTREQSLGRPHRSF 62 Query: 78 CDPLSVETEDYQRFWQHLRQGEVIAGTLQRRRIDGTIVWLEALCSPILDGSGQLRKVISV 137 C ++ Y+ W HL G +G ++R R DG+ WLEA SP++D G++ ++ + Sbjct: 63 CSARLADSARYREIWTHLCAGNAYSGVVERLRSDGSSCWLEATYSPVMDAQGRVMHILKI 122 Query: 138 AADISS-----HIDDSHHSNNVLSALNRSLGVVELSPQGMILAANRNYLKTMEYLADDLI 192 A D++ H L A VV I+ N + + + +++ Sbjct: 123 ATDVTQRRAREQAQQEHLRRLSLVADASDTAVVISDGSSRIVHVNGGFTRMFGWRTEEVA 182 Query: 193 GQ-PHSVLCPPGYADSEEYAIFWR-DVCSGRFFSGRFPRRTRSGRTIWWEATYSPLLDPQ 250 G+ P ++L P SE + +R ++ +G+ + G+ W + +P+LD Sbjct: 183 GRMPIALLAPQA---SEAFVDHYRAELRAGQPVGREEIVVGKHGQRYWAKVISNPILDAD 239 Query: 251 GKAERVICIGNDVT 264 G+ + + + D+T Sbjct: 240 GRWQYTVSMLTDIT 253 >ref|ZP_07046685.1| diguanylate cyclase/phosphodiesterase [Comamonas testosteroni S44] gb|EFI59804.1| diguanylate cyclase/phosphodiesterase [Comamonas testosteroni S44] Length = 860 Score = 905 bits (2339), Expect = 0.0, Method: Composition-based stats. Identities = 461/851 (54%), Positives = 589/851 (69%), Gaps = 7/851 (0%) Query: 155 LSALNRSLGVVELSPQGMILAANRNYLKTMEYLADDLIGQPHSVLCPPGYADSEEYAIFW 214 L AL+R + + E G + AN NYL+ M + + +G+PH C ADS Y W Sbjct: 10 LQALDRVMAMAEFGLDGSLHRANDNYLQLMGFTREQSLGRPHRSFCSARLADSARYREIW 69 Query: 215 RDVCSGRFFSGRFPRRTRSGRTIWWEATYSPLLDPQGKAERVICIGNDVTYRVTREKSDR 274 +C+G +SG R G + W EATYSP++D QG+ ++ I DVT R RE++ + Sbjct: 70 THLCAGNAYSGVVERLRSDGSSCWLEATYSPVMDAQGRVIHILKIATDVTQRRAREQAQQ 129 Query: 275 EHLHLLSLGINETDNAVLITDEQNRIVFHNTGFTRMLGFQSQQTIGKLPRELFASHPTFV 334 EHL LSL + +D AV+I+D +RIV N GFTRM G+++++ G +P L A + Sbjct: 130 EHLRRLSLVADASDTAVVISDGSSRIVHVNGGFTRMFGWRTEEVAGLMPIALLAPQAS-E 188 Query: 335 KSIERCHGELLQGHSFHTEELIHDLHEQPVWISAVVNPILDVQGKLINAVCVMTDITNTK 394 ++ EL G EE++ H Q W + NPILD G+ V ++TDIT +K Sbjct: 189 AFVDHYRAELRAGQPVGREEIVVGKHGQRYWAKVISNPILDADGRWQYTVSMLTDITRSK 248 Query: 395 VHEVLQYRVLNAMARDSSLAEQMNLLCEEVERVAPEVIASVLRVDEQGRLHTLAAPSLPE 454 +HE LQ+RVL AMAR+ L E + ++C+EVER+APEV AS+L VD QG LH LA PSLP Sbjct: 249 MHEALQHRVLEAMARERPLVEVLEMVCQEVERIAPEVTASILEVDAQGLLHPLAGPSLPH 308 Query: 455 AYTRAIEGIQIGPLVGSCGSAAYTGESVVSEEIATDPHWADYKEIALSHGLQACWSTPIK 514 +Y+ ++G+ IGP VGSCG+AA+ V+ ++IA DP W D+K + L G +ACWSTPI Sbjct: 309 SYSALLDGVAIGPNVGSCGTAAWRNAPVLVDDIAHDPLWTDFKHLILPLGFKACWSTPIC 368 Query: 515 SADGKVIGTFGFYYRQPRA--PSAFHHLLVDAGLHLCALAIEREASRARIHQLAFYDSLT 572 ++ GK IGTF FYYR+ SAFH LVDA +HLCALA+ERE SRARI QLAFYD LT Sbjct: 369 NSGGKPIGTFAFYYREQLTGDASAFHQQLVDACIHLCALALERELSRARIRQLAFYDGLT 428 Query: 573 GLPNRSLLLVQAGQAIAAAERNRSMVAVMFIDLDRFKQVNDSLGHQAGDELLRTVATRLQ 632 GLPNRSLL +A QAIA+A N +AV+FIDLDRFKQVNDSLGH GDELLR VATRLQ Sbjct: 429 GLPNRSLLQAKADQAIASAAGNDEQLAVLFIDLDRFKQVNDSLGHPVGDELLRNVATRLQ 488 Query: 633 SQARRSDIVGRLSGDEFVVVLTQYDTDHLTEVVKRIQERLAMPCHINGTPLNPSASIGVA 692 R SDI GRLSGDEFV VL Q D +H+ ++R+QE LA P +I T L+ SAS+G+A Sbjct: 489 RVLRSSDIAGRLSGDEFVAVLPQCDAEHVANTIERLQELLAEPLNIADTSLSISASVGIA 548 Query: 693 IFPDNGHDIETLLHRADIAMYQAKAVNRGRFSFFSEEMNSLAQERLVLEGALRDALRGGS 752 +FP +G D+ETL+HRAD+AMYQAK+ RGRF FFS EMN LAQERL LE ALR AL+ G Sbjct: 549 MFPMDGRDMETLVHRADMAMYQAKSKGRGRFCFFSSEMNRLAQERLALETALRKALKSGG 608 Query: 753 FELHFQPQINLHDLSLHGVEALARWYDPQLGQISPGRFIPIAEECGLIGELGKWALEEAC 812 LH+QPQI + L+GVEALARW +LG+ISP RFIP+AEECGLI +LG WALEEAC Sbjct: 609 LRLHYQPQIEMATGRLYGVEALARWTHAELGEISPARFIPLAEECGLIADLGSWALEEAC 668 Query: 813 FQLADWRRRGIQVPSVSVNLSATNFHDLDLPQFLTGLLRRYSLAPTDLTLEITETVLMDT 872 QL+ WR +G++VP+VSVNLS ++FH+LDLP+ + L R L P DLTLE+TE++L+DT Sbjct: 669 RQLSQWRAKGMEVPAVSVNLSPSSFHNLDLPRMIADTLDRNDLVPQDLTLELTESILLDT 728 Query: 873 NPSTNQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQSFVRDMAKDEAVSAL 932 NPST +TI VHA G+ L+MDDFGTGYSSL YLR+LPVSELKLD+SFV D+ DEA AL Sbjct: 729 NPSTMKTINEVHAHGVRLSMDDFGTGYSSLSYLRRLPVSELKLDRSFVADLEHDEAARAL 788 Query: 933 TNAVIRIGESLKLTVVAEGVEDADQYRLLISQGCDVAQGYYFSRPMSADQLESWVRDYRG 992 ++A++ IG+SL LTVVAEGVE Q +L QG VAQG+ F+RP++ + E W+ Sbjct: 789 SSAILGIGKSLHLTVVAEGVETPTQSVMLCEQGYPVAQGFLFARPLTPKEFEHWL----A 844 Query: 993 ENAAPPGATGP 1003 N A P T P Sbjct: 845 ANPASPVPTVP 855 Score = 100 bits (249), Expect = 1e-18, Method: Composition-based stats. Identities = 66/245 (26%), Positives = 117/245 (47%), Gaps = 10/245 (4%) Query: 27 EEREALLGALWRSMAILEFAADGSILDANDKFLGMLGYQRHEVVGQHHRMLCDPLSVETE 86 E + A L AL R MA+ EF DGS+ AND +L ++G+ R + +G+ HR C ++ Sbjct: 4 ELQLAQLQALDRVMAMAEFGLDGSLHRANDNYLQLMGFTREQSLGRPHRSFCSARLADSA 63 Query: 87 DYQRFWQHLRQGEVIAGTLQRRRIDGTIVWLEALCSPILDGSGQLRKVISVAADISS--- 143 Y+ W HL G +G ++R R DG+ WLEA SP++D G++ ++ +A D++ Sbjct: 64 RYREIWTHLCAGNAYSGVVERLRSDGSSCWLEATYSPVMDAQGRVIHILKIATDVTQRRA 123 Query: 144 --HIDDSHHSNNVLSALNRSLGVVELSPQGMILAANRNYLKTMEYLADDLIG-QPHSVLC 200 H L A VV I+ N + + + +++ G P ++L Sbjct: 124 REQAQQEHLRRLSLVADASDTAVVISDGSSRIVHVNGGFTRMFGWRTEEVAGLMPIALLA 183 Query: 201 PPGYADSEEYAIFWR-DVCSGRFFSGRFPRRTRSGRTIWWEATYSPLLDPQGKAERVICI 259 P SE + +R ++ +G+ + G+ W + +P+LD G+ + + + Sbjct: 184 PQA---SEAFVDHYRAELRAGQPVGREEIVVGKHGQRYWAKVISNPILDADGRWQYTVSM 240 Query: 260 GNDVT 264 D+T Sbjct: 241 LTDIT 245 >ref|ZP_03541929.1| diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) [Comamonas testosteroni KF-1] gb|EED66215.1| diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) [Comamonas testosteroni KF-1] Length = 868 Score = 894 bits (2310), Expect = 0.0, Method: Composition-based stats. Identities = 457/852 (53%), Positives = 587/852 (68%), Gaps = 11/852 (1%) Query: 155 LSALNRSLGVVELSPQGMILAANRNYLKTMEYLADDLIGQPHSVLCPPGYADSEEYAIFW 214 L AL+R + + E G++ AN NYL+ M + +G+ H C A S Y W Sbjct: 18 LQALDRVMAMAEFGLDGVLCRANDNYLQLMGLTREQCLGRAHRSFCSAQLAGSARYREIW 77 Query: 215 RDVCSGRFFSGRFPRRTRSGRTIWWEATYSPLLDPQGKAERVICIGNDVTYRVTREKSDR 274 +C+G +SG R G + W EATYSP++D QG+ ++ I DVT R E++ + Sbjct: 78 AHLCAGNAYSGVVERLRSDGSSCWLEATYSPVMDAQGRVAHILKIATDVTQRRALEQAQQ 137 Query: 275 EHLHLLSLGINETDNAVLITDEQNRIVFHNTGFTRMLGFQSQQTIGKLPRELFASH--PT 332 EHL LSL + +D AV+I+D +RIV N GFTRM G+++++ G+ P L A T Sbjct: 138 EHLRRLSLVADASDTAVVISDGNSRIVHVNGGFTRMFGWRTEEVAGRTPIALLAPQVSET 197 Query: 333 FVKSIERCHGELLQGHSFHTEELIHDLHEQPVWISAVVNPILDVQGKLINAVCVMTDITN 392 FV +R +L G EE++ H Q W + NPILD G V ++TDIT Sbjct: 198 FV---DRYRSDLRAGQPVGREEIVVGKHGQRYWAKVISNPILDAGGCWQYTVSMLTDITR 254 Query: 393 TKVHEVLQYRVLNAMARDSSLAEQMNLLCEEVERVAPEVIASVLRVDEQGRLHTLAAPSL 452 +K+HE LQ+RVL AMAR+ L E + ++C EVER+APEV AS+L VD QG LH LA PSL Sbjct: 255 SKMHEALQHRVLEAMARERPLVEVLEMVCLEVERIAPEVTASILEVDAQGLLHPLAGPSL 314 Query: 453 PEAYTRAIEGIQIGPLVGSCGSAAYTGESVVSEEIATDPHWADYKEIALSHGLQACWSTP 512 P++Y+ ++G+ +GP VGSCG+AA+ V ++I DP WAD++ + L G +ACWSTP Sbjct: 315 PQSYSALLDGLAVGPKVGSCGTAAWLNAPVQVDDIGQDPLWADFRHLILPLGFKACWSTP 374 Query: 513 IKSADGKVIGTFGFYYRQPR--APSAFHHLLVDAGLHLCALAIEREASRARIHQLAFYDS 570 I +++GK IGTF FYYR+P SAFH LVDA +HLCALA+ERE SRARI QLAFYD Sbjct: 375 ICNSEGKPIGTFAFYYREPLTGGASAFHQQLVDACIHLCALALERELSRARIRQLAFYDG 434 Query: 571 LTGLPNRSLLLVQAGQAIAAAERNRSMVAVMFIDLDRFKQVNDSLGHQAGDELLRTVATR 630 LTGLPNRSLL +A QAIA+A + + +AV+FIDLDRFKQVNDSLGH GDELLR+VA R Sbjct: 435 LTGLPNRSLLQAKADQAIASAATHDAQLAVLFIDLDRFKQVNDSLGHPVGDELLRSVAAR 494 Query: 631 LQSQARRSDIVGRLSGDEFVVVLTQYDTDHLTEVVKRIQERLAMPCHINGTPLNPSASIG 690 LQ R SDI GRLSGDEFV VL D +++ ++R+QE LA P +I T L SASIG Sbjct: 495 LQRVLRSSDIAGRLSGDEFVAVLPHCDAEYVANTIERLQELLAEPLNIADTSLAISASIG 554 Query: 691 VAIFPDNGHDIETLLHRADIAMYQAKAVNRGRFSFFSEEMNSLAQERLVLEGALRDALRG 750 +A+FP +G D+ETL+HRAD+AMYQAK+ RGRF FFS EMN LAQERL LE ALR AL+ Sbjct: 555 IAMFPSDGRDMETLVHRADMAMYQAKSKGRGRFCFFSSEMNRLAQERLALETALRKALKS 614 Query: 751 GSFELHFQPQINLHDLSLHGVEALARWYDPQLGQISPGRFIPIAEECGLIGELGKWALEE 810 G LH+QPQI + L+GVEALARW +LG+ISP RFIP+AEECGLI +LG WALEE Sbjct: 615 GGLRLHYQPQIEMATGRLYGVEALARWSHAELGEISPARFIPLAEECGLIADLGSWALEE 674 Query: 811 ACFQLADWRRRGIQVPSVSVNLSATNFHDLDLPQFLTGLLRRYSLAPTDLTLEITETVLM 870 AC QL+ WR +G++VP+VSVNLS ++FH+LDLP+ + L R L P DLTLE+TE++L+ Sbjct: 675 ACRQLSQWRAKGLEVPAVSVNLSPSSFHNLDLPRMIADTLDRNDLVPQDLTLELTESILL 734 Query: 871 DTNPSTNQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQSFVRDMAKDEAVS 930 DTNPST +TI VHA G+ L+MDDFGTGYSSL YLR+LPVSELKLD+SFV D+ DEA Sbjct: 735 DTNPSTMKTINEVHAHGVRLSMDDFGTGYSSLSYLRRLPVSELKLDRSFVADLEHDEAAR 794 Query: 931 ALTNAVIRIGESLKLTVVAEGVEDADQYRLLISQGCDVAQGYYFSRPMSADQLESWVRDY 990 AL++A++ IG+SL LTVVAEGVE Q +L QG VAQG+ F+RP++ + E W+ Sbjct: 795 ALSSAILGIGKSLHLTVVAEGVETPTQSVMLCEQGYPVAQGFLFARPLAPQEFEHWL--- 851 Query: 991 RGENAAPPGATG 1002 NAA P T Sbjct: 852 -AANAASPVQTA 862 Score = 97.4 bits (241), Expect = 1e-17, Method: Composition-based stats. Identities = 68/254 (26%), Positives = 118/254 (46%), Gaps = 10/254 (3%) Query: 18 QGDHQRLREEEREALLGALWRSMAILEFAADGSILDANDKFLGMLGYQRHEVVGQHHRML 77 +GD + E + A L AL R MA+ EF DG + AND +L ++G R + +G+ HR Sbjct: 3 EGDIGAMPPELQLARLQALDRVMAMAEFGLDGVLCRANDNYLQLMGLTREQCLGRAHRSF 62 Query: 78 CDPLSVETEDYQRFWQHLRQGEVIAGTLQRRRIDGTIVWLEALCSPILDGSGQLRKVISV 137 C + Y+ W HL G +G ++R R DG+ WLEA SP++D G++ ++ + Sbjct: 63 CSAQLAGSARYREIWAHLCAGNAYSGVVERLRSDGSSCWLEATYSPVMDAQGRVAHILKI 122 Query: 138 AADISS-----HIDDSHHSNNVLSALNRSLGVVELSPQGMILAANRNYLKTMEYLADDLI 192 A D++ H L A VV I+ N + + + +++ Sbjct: 123 ATDVTQRRALEQAQQEHLRRLSLVADASDTAVVISDGNSRIVHVNGGFTRMFGWRTEEVA 182 Query: 193 GQ-PHSVLCPPGYADSEEYAIFWR-DVCSGRFFSGRFPRRTRSGRTIWWEATYSPLLDPQ 250 G+ P ++L P SE + +R D+ +G+ + G+ W + +P+LD Sbjct: 183 GRTPIALLAP---QVSETFVDRYRSDLRAGQPVGREEIVVGKHGQRYWAKVISNPILDAG 239 Query: 251 GKAERVICIGNDVT 264 G + + + D+T Sbjct: 240 GCWQYTVSMLTDIT 253 >ref|ZP_06685654.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor [Achromobacter piechaudii ATCC 43553] gb|EFF77500.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor [Achromobacter piechaudii ATCC 43553] Length = 870 Score = 886 bits (2290), Expect = 0.0, Method: Composition-based stats. Identities = 451/844 (53%), Positives = 581/844 (68%), Gaps = 12/844 (1%) Query: 150 HSNNVLSALNRSLGVVELSPQGMILAANRNYLKTMEYLADDLIGQPHSVLCPP-----GY 204 H++N L++++R+L + + G +L N N+L + Y ++ + H +LC G Sbjct: 32 HADNYLASMDRALLLFDFDADGALLKVNANFLAAVGYTREEALSLRHDMLCDSMDEGKGI 91 Query: 205 ADSEEYAIFWRDVCSGRFFSGRFPRRTRSGRTIWWEATYSPLLDPQGKAERVICIGNDVT 264 A E W + G FSG R + G ++W EATY PL D GK RV + V+ Sbjct: 92 ASGER---IWTRLRRGEHFSGTCRYRMKDGSSLWIEATYLPLGDKDGKISRV----SVVS 144 Query: 265 YRVTREKSDREHLHLLSLGINETDNAVLITDEQNRIVFHNTGFTRMLGFQSQQTIGKLPR 324 + + +E + LL LGINET NAV ++ RIV+ N GF RMLGF + + Sbjct: 145 RKSIADAERQEEVRLLLLGINETGNAVAVSGCDGRIVYVNDGFQRMLGFARGDALNQELG 204 Query: 325 ELFASHPTFVKSIERCHGELLQGHSFHTEELIHDLHEQPVWISAVVNPILDVQGKLINAV 384 EL A + E + FH + L++D + +P+W+S + N + D +G L+N V Sbjct: 205 ELLAGGRPDGGTREELDRRIACREGFHKDVLVYDRYGKPLWVSVMANSVFDERGTLVNIV 264 Query: 385 CVMTDITNTKVHEVLQYRVLNAMARDSSLAEQMNLLCEEVERVAPEVIASVLRVDEQGRL 444 V+TDIT TKVHEVLQ RVL AM ++S+ E MN++C EVER+APEV ASVLRVD+ G L Sbjct: 265 DVLTDITPTKVHEVLQRRVLQAMVNEASVVEVMNMVCREVERLAPEVAASVLRVDDSGTL 324 Query: 445 HTLAAPSLPEAYTRAIEGIQIGPLVGSCGSAAYTGESVVSEEIATDPHWADYKEIALSHG 504 LAAPS+P+AY+ A+EG++IGP VG+CG+AA+ G V+ +IATDP W DY+ + L Sbjct: 325 RPLAAPSMPDAYSEALEGMKIGPNVGACGTAAFLGRPVIVPDIATDPLWDDYRHLPLPED 384 Query: 505 LQACWSTPIKSADGKVIGTFGFYYRQPRAPSAFHHLLVDAGLHLCALAIEREASRARIHQ 564 ++ACWS+PIKS+DG+VIGTFGFY+R+ R P FHH LVD ++LCALA+ERE +RARI Q Sbjct: 385 IKACWSSPIKSSDGRVIGTFGFYFRERRLPDDFHHRLVDVCVYLCALALEREEARARIRQ 444 Query: 565 LAFYDSLTGLPNRSLLLVQAGQAIAAAERNRSMVAVMFIDLDRFKQVNDSLGHQAGDELL 624 LAFYD LTGLPNR+LLL QA Q+IA AE R VAV+F+DLDRFKQVND+LGH GD LL Sbjct: 445 LAFYDELTGLPNRNLLLAQAEQSIARAEPERKRVAVLFLDLDRFKQVNDTLGHPIGDALL 504 Query: 625 RTVATRLQSQARRSDIVGRLSGDEFVVVLTQYDTDHLTEVVKRIQERLAMPCHINGTPLN 684 R VA RL AR +DIVGRLSGDEFV+++ ++ LT + I LA P + G LN Sbjct: 505 RDVAQRLCRLARATDIVGRLSGDEFVMLMPDFEHGRLTAAAEHILVALAQPFSVGGVTLN 564 Query: 685 PSASIGVAIFPDNGHDIETLLHRADIAMYQAKAVNRGRFSFFSEEMNSLAQERLVLEGAL 744 PS SIG+++FP+NG D++TLL AD+AMYQAK R R SFFS EMN AQERL LE AL Sbjct: 565 PSVSIGISVFPENGRDMDTLLRHADMAMYQAKTAGRNRISFFSAEMNRQAQERLALEAAL 624 Query: 745 RDALRGGSFELHFQPQINLHDLSLHGVEALARWYDPQLGQISPGRFIPIAEECGLIGELG 804 RDAL + LH+QPQ+ L + SL+GVEALARW P LG ISP RF+P+AEECGLIG+LG Sbjct: 625 RDALEAKALRLHYQPQVGLKNGSLYGVEALARWRHPTLGDISPVRFVPLAEECGLIGDLG 684 Query: 805 KWALEEACFQLADWRRRGIQVPSVSVNLSATNFHDLDLPQFLTGLLRRYSLAPTDLTLEI 864 WAL EAC QLA WR G++VPSVSVNLSATNFH+L+LP+ + L + LAP DL LEI Sbjct: 685 DWALREACSQLAIWRSNGLRVPSVSVNLSATNFHNLNLPRMIAATLAEFGLAPADLMLEI 744 Query: 865 TETVLMDTNPSTNQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSELKLDQSFVRDMA 924 TE V++D T +TIA +H LG+ L+MDDFGTGYSSLG+LR+L V ELKLD+SFV+ + Sbjct: 745 TEGVVLDATAGTLRTIAELHRLGVRLSMDDFGTGYSSLGHLRRLIVDELKLDRSFVQGLE 804 Query: 925 KDEAVSALTNAVIRIGESLKLTVVAEGVEDADQYRLLISQGCDVAQGYYFSRPMSADQLE 984 D+A ALT+AVIRIGESL L VVAEGVE+ +Q R LI QGC QG+ FS P+ A +LE Sbjct: 805 SDDAARALTSAVIRIGESLSLPVVAEGVENEEQRRFLIEQGCAAGQGFLFSPPLPAGELE 864 Query: 985 SWVR 988 W+R Sbjct: 865 EWLR 868 Score = 87.4 bits (215), Expect = 1e-14, Method: Composition-based stats. Identities = 62/251 (24%), Positives = 108/251 (43%), Gaps = 3/251 (1%) Query: 33 LGALWRSMAILEFAADGSILDANDKFLGMLGYQRHEVVGQHHRMLCDPLS--VETEDYQR 90 L ++ R++ + +F ADG++L N FL +GY R E + H MLCD + +R Sbjct: 37 LASMDRALLLFDFDADGALLKVNANFLAAVGYTREEALSLRHDMLCDSMDEGKGIASGER 96 Query: 91 FWQHLRQGEVIAGTLQRRRIDGTIVWLEALCSPILDGSGQLRKVISVAADISSHIDDSHH 150 W LR+GE +GT + R DG+ +W+EA P+ D G++ +V V+ + + Sbjct: 97 IWTRLRRGEHFSGTCRYRMKDGSSLWIEATYLPLGDKDGKISRVSVVSRKSIADAERQEE 156 Query: 151 SNNVLSALNRSLGVVELSP-QGMILAANRNYLKTMEYLADDLIGQPHSVLCPPGYADSEE 209 +L +N + V +S G I+ N + + + + D + Q L G D Sbjct: 157 VRLLLLGINETGNAVAVSGCDGRIVYVNDGFQRMLGFARGDALNQELGELLAGGRPDGGT 216 Query: 210 YAIFWRDVCSGRFFSGRFPRRTRSGRTIWWEATYSPLLDPQGKAERVICIGNDVTYRVTR 269 R + F R G+ +W + + D +G ++ + D+T Sbjct: 217 REELDRRIACREGFHKDVLVYDRYGKPLWVSVMANSVFDERGTLVNIVDVLTDITPTKVH 276 Query: 270 EKSDREHLHLL 280 E R L + Sbjct: 277 EVLQRRVLQAM 287