BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2509.p (446 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003774150.1| tRNA modifying 2-methylthioadenine synthetas... 867 0.0 ref|YP_004754255.1| tRNA-i(6)A37 methylthiotransferase [Collimon... 762 0.0 ref|YP_001098733.1| (dimethylallyl)adenosine tRNA methylthiotran... 734 0.0 ref|YP_001352121.1| tRNA modifying enzyme [Janthinobacterium sp.... 730 0.0 ref|YP_004361962.1| 2-methylthioadenine synthetase [Burkholderia... 679 0.0 >ref|YP_003774150.1| tRNA modifying 2-methylthioadenine synthetase [Herbaspirillum seropedicae SmR1] gb|ADJ62242.1| tRNA modifying 2-methylthioadenine synthetase protein [Herbaspirillum seropedicae SmR1] Length = 446 Score = 867 bits (2239), Expect = 0.0, Method: Composition-based stats. Identities = 427/446 (95%), Positives = 440/446 (98%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLHVAEGLVKTDRPEEADVILLNTCSIREKAQEKVF 60 MQKKVFIKTFGCQMNEYDSDKMADVL+ ++GL+KTDRPEEADVILLNTCSIREKAQEKVF Sbjct: 1 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEEADVILLNTCSIREKAQEKVF 60 Query: 61 SDLGRLRALKKDKPDLLIGVGGCVASQEGDAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGRLRALKK+ P LLIGVGGCVASQEG+AIIKRAPFVDMVFGPQTLHRLPQMISERRY Sbjct: 61 SDLGRLRALKKNNPALLIGVGGCVASQEGEAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED Sbjct: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH Sbjct: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 Query: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI Sbjct: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 Query: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSPRPGTPAASLADDTPQEVKLA 360 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFS RPGTPAA+L DDTPQEVKLA Sbjct: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSARPGTPAANLPDDTPQEVKLA 360 Query: 361 RLQRLQAALNDNAAKISAAQVGTVQKVLVEGISKRREHELQGRTESNRVVNFDGGPNGQR 420 RLQRLQAALNDNAA+ISAAQVGTVQ+VLVEG+SKRREHELQGRTESNRVVNFDGGPNGQR Sbjct: 361 RLQRLQAALNDNAARISAAQVGTVQRVLVEGVSKRREHELQGRTESNRVVNFDGGPNGQR 420 Query: 421 LIGEIIEVRVTEAFPFSLRGEIVTRH 446 LIG+IIEV +TEAFPFSLRGEIVT+H Sbjct: 421 LIGQIIEVSITEAFPFSLRGEIVTQH 446 >ref|YP_004754255.1| tRNA-i(6)A37 methylthiotransferase [Collimonas fungivorans Ter331] gb|AEK63432.1| tRNA-i(6)A37 methylthiotransferase [Collimonas fungivorans Ter331] Length = 450 Score = 762 bits (1967), Expect = 0.0, Method: Composition-based stats. Identities = 365/443 (82%), Positives = 408/443 (92%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLHVAEGLVKTDRPEEADVILLNTCSIREKAQEKVF 60 MQKKVFIKTFGCQMNEYDSDKMADVL+ ++GL+KTDRPE+ADVILLNTCS+REKAQEKVF Sbjct: 5 MQKKVFIKTFGCQMNEYDSDKMADVLNASDGLIKTDRPEDADVILLNTCSVREKAQEKVF 64 Query: 61 SDLGRLRALKKDKPDLLIGVGGCVASQEGDAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGRLR LK++ PDLLIGVGGCVASQEG AI+KRAP+VDMVFGPQTLHRLP+MIS+RR Sbjct: 65 SDLGRLRELKRNNPDLLIGVGGCVASQEGAAIVKRAPYVDMVFGPQTLHRLPEMISQRRA 124 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 +G QVDISFPEIEKFDH+PPA+VEGATA+VSIMEGCSKYCSYCVVPYTRGEEVSRRFED Sbjct: 125 TGHSQVDISFPEIEKFDHMPPAKVEGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 184 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 VLTEVAGLA+QGVKEI LLGQNVNA+RG M DGEIADFALLIEYIAE+PGIERIRFVTSH Sbjct: 185 VLTEVAGLAEQGVKEITLLGQNVNAFRGAMADGEIADFALLIEYIAEIPGIERIRFVTSH 244 Query: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 PKEF+QRLIDTYAKVPKL +HLYLPAQHGSDR L+AMKRGYT LEYKSVIRR+R+VRP+I Sbjct: 245 PKEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILSAMKRGYTVLEYKSVIRRLRQVRPNI 304 Query: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSPRPGTPAASLADDTPQEVKLA 360 T+ SDFIVGFPGET+ DFEALMKL+ D+G+DNSFSFIFSPRPGTPAA+L DDTP EVKL Sbjct: 305 TISSDFIVGFPGETDADFEALMKLITDIGYDNSFSFIFSPRPGTPAANLEDDTPHEVKLK 364 Query: 361 RLQRLQAALNDNAAKISAAQVGTVQKVLVEGISKRREHELQGRTESNRVVNFDGGPNGQR 420 RLQ LQA + N AKISAA VG+VQ++LVEG SK+ ELQGRTE+NRVVNF+ GPNG R Sbjct: 365 RLQHLQATVEANMAKISAAMVGSVQRILVEGPSKKNPAELQGRTENNRVVNFNAGPNGAR 424 Query: 421 LIGEIIEVRVTEAFPFSLRGEIV 443 LIGE+I+V +TE ++LRGEIV Sbjct: 425 LIGELIDVTITETLTYTLRGEIV 447 >ref|YP_001098733.1| (dimethylallyl)adenosine tRNA methylthiotransferase [Herminiimonas arsenicoxydans] sp|A4G267|MIAB_HERAR RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase emb|CAL60604.1| putative tRNA-i(6)A37 thiotransferase enzyme MiaB [Herminiimonas arsenicoxydans] Length = 446 Score = 734 bits (1894), Expect = 0.0, Method: Composition-based stats. Identities = 348/446 (78%), Positives = 402/446 (90%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLHVAEGLVKTDRPEEADVILLNTCSIREKAQEKVF 60 MQKK++IKTFGCQMNEYDSDKMADVL+ ++GL+KTD+ E+ADVILLNTCS+REKAQEKVF Sbjct: 1 MQKKIYIKTFGCQMNEYDSDKMADVLNASDGLIKTDKAEDADVILLNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKDKPDLLIGVGGCVASQEGDAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGRLR LK KPDL+IGVGGCVASQEGDAIIKRAP+VD+VFGPQTLHRLP+M+ +RR Sbjct: 61 SDLGRLRELKLLKPDLMIGVGGCVASQEGDAIIKRAPYVDLVFGPQTLHRLPEMLKQRRS 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 +GR QVDISFPEIEKFDH+PPA+VEGATA+VSIMEGCSKYCSYCVVPYTRGEEVSRRF+D Sbjct: 121 TGRSQVDISFPEIEKFDHMPPAKVEGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRRFDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 VLTEVAGL QGVKEI LLGQNVNAYRG M DGEIADFALLIEYIAE+ GIERIR+VTSH Sbjct: 181 VLTEVAGLEAQGVKEITLLGQNVNAYRGEMADGEIADFALLIEYIAEMEGIERIRYVTSH 240 Query: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 PKEF+QRLIDTYAKVPKL +HLYLPAQHGSDR LAAMKRGYT+LEYKS++RR+R VRP+I Sbjct: 241 PKEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILAAMKRGYTSLEYKSILRRLRAVRPNI 300 Query: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSPRPGTPAASLADDTPQEVKLA 360 ++ SDFIVGFPGET+EDFEA+MKL+ D+G+DNSFSFIFSPRPGTPAA+LADDTP EVKL Sbjct: 301 SISSDFIVGFPGETDEDFEAMMKLIDDIGYDNSFSFIFSPRPGTPAANLADDTPHEVKLK 360 Query: 361 RLQRLQAALNDNAAKISAAQVGTVQKVLVEGISKRREHELQGRTESNRVVNFDGGPNGQR 420 RLQRLQA ++ N + S VG+VQ++LVEG SK+ ELQGRTE+NRVVNF GPN Sbjct: 361 RLQRLQAVIDQNTRRYSDEMVGSVQRILVEGPSKKDADELQGRTENNRVVNFSAGPNAAH 420 Query: 421 LIGEIIEVRVTEAFPFSLRGEIVTRH 446 LIG+++ V +T++ ++LRGEIV + Sbjct: 421 LIGQMVNVNITQSHAYTLRGEIVVKQ 446 >ref|YP_001352121.1| tRNA modifying enzyme [Janthinobacterium sp. Marseille] sp|A6SV24|MIAB_JANMA RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB; AltName: Full=tRNA-i(6)A37 methylthiotransferase gb|ABR88458.1| tRNA modifying enzyme [Janthinobacterium sp. Marseille] Length = 446 Score = 730 bits (1885), Expect = 0.0, Method: Composition-based stats. Identities = 347/446 (77%), Positives = 400/446 (89%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLHVAEGLVKTDRPEEADVILLNTCSIREKAQEKVF 60 MQKK++IKTFGCQMNEYDSDKM DVL+ +EGL+KTD PE+ADVILLNTCS+REKAQEKVF Sbjct: 1 MQKKIYIKTFGCQMNEYDSDKMVDVLNASEGLIKTDTPEDADVILLNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKDKPDLLIGVGGCVASQEGDAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGRLR LK + PDL+IGVGGCVASQEGDAI+KRAP+VD+VFGPQTLHRLP+M+ +RR Sbjct: 61 SDLGRLRELKFNNPDLVIGVGGCVASQEGDAIVKRAPYVDLVFGPQTLHRLPEMLKQRRS 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 +GR QVDISFPEIEKFDH+PPA+VEGATA+VSIMEGCSKYCSYCVVPYTRGEEVSRRFED Sbjct: 121 TGRSQVDISFPEIEKFDHMPPAKVEGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDGEIADFALLIEYIAELPGIERIRFVTSH 240 VL EVAGL QGVKEI LLGQNVNAYRG M DGEIADFALLIEYIAEL GIERIRFVTSH Sbjct: 181 VLAEVAGLEAQGVKEITLLGQNVNAYRGEMADGEIADFALLIEYIAELEGIERIRFVTSH 240 Query: 241 PKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRPDI 300 PKEF+QRLID YAKVPKL NHLYLPAQHGSDR LAAMKRGYT+LEYKS++RR+R+VRP+I Sbjct: 241 PKEFTQRLIDAYAKVPKLVNHLYLPAQHGSDRILAAMKRGYTSLEYKSILRRLREVRPNI 300 Query: 301 TVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSPRPGTPAASLADDTPQEVKLA 360 ++ SDFIVGFPGET+ DFEA+MKL+ D+G+DNSFSFIFSPRPGTPAA+L DDTP EVKL Sbjct: 301 SISSDFIVGFPGETDADFEAMMKLINDIGYDNSFSFIFSPRPGTPAANLEDDTPHEVKLQ 360 Query: 361 RLQRLQAALNDNAAKISAAQVGTVQKVLVEGISKRREHELQGRTESNRVVNFDGGPNGQR 420 RLQRLQA ++ N + S VGTVQ++LVEG SK+ ELQGRTE+NRVVNF G +G R Sbjct: 361 RLQRLQAVIDQNTRRYSDEMVGTVQRILVEGPSKKDPDELQGRTENNRVVNFAAGEHGAR 420 Query: 421 LIGEIIEVRVTEAFPFSLRGEIVTRH 446 LIG++++V + ++F ++LRGEI+ + Sbjct: 421 LIGQMVDVNIVQSFAYTLRGEIIVKQ 446 >ref|YP_004361962.1| 2-methylthioadenine synthetase [Burkholderia gladioli BSR3] gb|AEA62006.1| 2-methylthioadenine synthetase [Burkholderia gladioli BSR3] Length = 457 Score = 679 bits (1752), Expect = 0.0, Method: Composition-based stats. Identities = 323/449 (71%), Positives = 386/449 (85%), Gaps = 3/449 (0%) Query: 1 MQKKVFIKTFGCQMNEYDSDKMADVLHVAEGLVKTDRPEEADVILLNTCSIREKAQEKVF 60 M KK++IKTFGCQMNEYDSDKM DVL+ +EGLVKTDRPE+ADVIL NTCS+REKAQEKVF Sbjct: 1 MTKKIYIKTFGCQMNEYDSDKMVDVLNASEGLVKTDRPEDADVILFNTCSVREKAQEKVF 60 Query: 61 SDLGRLRALKKDKPDLLIGVGGCVASQEGDAIIKRAPFVDMVFGPQTLHRLPQMISERRY 120 SDLGR+R LK+ P+L++GVGGCVASQEG AI+ RAP+VD+VFGPQTLHRLPQMI RR Sbjct: 61 SDLGRVRELKEANPNLIVGVGGCVASQEGAAIVARAPYVDVVFGPQTLHRLPQMIDARRA 120 Query: 121 SGRPQVDISFPEIEKFDHLPPARVEGATAYVSIMEGCSKYCSYCVVPYTRGEEVSRRFED 180 SGR QVDISFPEIEKFDHLPPARVEG +A+VSIMEGCSKYCSYCVVPYTRG+EVSR +D Sbjct: 121 SGRSQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD 180 Query: 181 VLTEVAGLADQGVKEIMLLGQNVNAYRGVMEDG--EIADFALLIEYIAELPGIERIRFVT 238 VLTE+AGLADQGV+E+ LLGQNVNAYRG + G EIADFA LIEY+AELPGIERIR+ T Sbjct: 181 VLTEIAGLADQGVREVTLLGQNVNAYRGALTQGSEEIADFATLIEYVAELPGIERIRYTT 240 Query: 239 SHPKEFSQRLIDTYAKVPKLCNHLYLPAQHGSDRTLAAMKRGYTALEYKSVIRRMRKVRP 298 SHPKEFSQRL+D YA+VPKL NHL+LP QHGSDR L AMKRGYT LEYKS+IR++R +RP Sbjct: 241 SHPKEFSQRLLDVYARVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSLIRKLRAIRP 300 Query: 299 DITVQSDFIVGFPGETEEDFEALMKLVADVGFDNSFSFIFSPRPGTPAASLADDTPQEVK 358 D+++ +D I+GFPGETE DF+ M+LV ++ +D SFSFI+SPRPGTPAA+L DDTP+EVK Sbjct: 301 DLSLSTDIIIGFPGETEADFDKTMQLVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVK 360 Query: 359 LARLQRLQAALNDNAAKISAAQVGTVQKVLVEGISKRREHELQGRTESNRVVNFDGG-PN 417 L RLQ LQA + +N A+ISA+ VG ++++LVEG S++ +EL GRTE+NRVVNF + Sbjct: 361 LKRLQHLQATIEENVARISASMVGRIERILVEGPSRKDPNELAGRTENNRVVNFPAPLAS 420 Query: 418 GQRLIGEIIEVRVTEAFPFSLRGEIVTRH 446 RLIG++I+VR+ A+P SLRGE+V H Sbjct: 421 HARLIGQMIDVRINHAYPHSLRGELVLAH 449