BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2414.p (356 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoredu... 674 0.0 ref|YP_004681513.1| phenylacetic acid degradation NADH oxidoredu... 488 e-136 ref|YP_160277.1| hypothetical protein ebA5725 [Aromatoleum aroma... 482 e-134 ref|YP_840878.1| phenylacetic acid degradation protein E,flavodo... 478 e-133 ref|YP_297941.1| phenylacetate-CoA oxygenase/reductase subunit P... 477 e-132 >ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoreductase [Herbaspirillum seropedicae SmR1] gb|ADJ65599.1| phenylacetic acid degradation NADH oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 356 Score = 674 bits (1740), Expect = 0.0, Method: Composition-based stats. Identities = 321/356 (90%), Positives = 338/356 (94%) Query: 1 MSKFYPLTISGVKQETRDTIVVSFAVPPELQDTFSYQQGQHLTLRSEIKGEDLRRSYSIC 60 MSKFYPLTIS VKQETRDTIVVSFAVP ELQDTFSYQQGQHLTLRSEI GEDLRRSYSIC Sbjct: 1 MSKFYPLTISDVKQETRDTIVVSFAVPAELQDTFSYQQGQHLTLRSEINGEDLRRSYSIC 60 Query: 61 SAVQDDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNVPLDAANRKHYLAFAA 120 SAVQ+ QLRVAIKR GG+FS WAN++ PGQ + VMPPMGHFNVPL+A NRKHYLAFAA Sbjct: 61 SAVQERQLRVAIKRAPGGLFSNWANESFVPGQRIDVMPPMGHFNVPLEAGNRKHYLAFAA 120 Query: 121 GSGITPMISIIKTTLLAEPHSRFTLVYANRASSSVIFKEDITDLKDAYLERLNVIWVMSR 180 GSGITPM+SIIKTTLL+EPHS FTLVYANRASSSVIFKE++TDLKDAYLER NV++VMSR Sbjct: 121 GSGITPMMSIIKTTLLSEPHSHFTLVYANRASSSVIFKEELTDLKDAYLERFNVVYVMSR 180 Query: 181 EQQDVELFNGRIDRSKCDAFFASWIDLKDVDAAFLCGPEEMVQAVSDSLQAHGLPKTQIK 240 EQQDVELFNGRIDR+KCDAFFASWIDLKDVDAAFLCGPEEMVQAV+ SLQAHGLPKTQIK Sbjct: 181 EQQDVELFNGRIDRAKCDAFFASWIDLKDVDAAFLCGPEEMVQAVAASLQAHGLPKTQIK 240 Query: 241 TELFAASAPTRAHASRPVVGKKECEVTVIVDGYHNVFTMDKEKESVLDAGLKHGIDLRYS 300 TELFAAS P RAHA+R VVGK+ECEVTVIVDGYHNVFTMDKEKESVL+AGLKHGIDLRYS Sbjct: 241 TELFAASTPPRAHAARTVVGKQECEVTVIVDGYHNVFTMDKEKESVLEAGLKHGIDLRYS 300 Query: 301 CKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQDN 356 CKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQDN Sbjct: 301 CKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQDN 356 >ref|YP_004681513.1| phenylacetic acid degradation NADH oxidoreductase PaaE [Cupriavidus necator N-1] gb|AEI80281.1| phenylacetic acid degradation NADH oxidoreductase PaaE [Cupriavidus necator N-1] Length = 358 Score = 488 bits (1256), Expect = e-136, Method: Composition-based stats. Identities = 230/358 (64%), Positives = 287/358 (80%), Gaps = 4/358 (1%) Query: 1 MSKFYPLTISGVKQETRDTIVVSFAVPPELQDTFSYQQGQHLTLRSEIKGEDLRRSYSIC 60 MSKF+ LT++ V +ETRD + V+FAVP EL DT+ Y QGQHLTLR+ I GED+RRSYSIC Sbjct: 1 MSKFHELTVASVTRETRDAVAVTFAVPDELADTYRYIQGQHLTLRTGIGGEDVRRSYSIC 60 Query: 61 SAVQDDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNVPLDAANRKHYLAFAA 120 SAVQD QLRVAIKR GG+FS WAN+ L+PG L+VMPP GHF+VPL AA +HY+AFAA Sbjct: 61 SAVQDAQLRVAIKRVDGGLFSNWANEQLQPGMKLEVMPPSGHFHVPLSAAQARHYVAFAA 120 Query: 121 GSGITPMISIIKTTLLAEPHSRFTLVYANRASSSVIFKEDITDLKDAYLERLNVIWVMSR 180 GSGITPM+SIIKTTL AEP SRFTL Y NRASSSV+FKE++ DLKD YL+R N+++V+SR Sbjct: 121 GSGITPMLSIIKTTLQAEPESRFTLFYGNRASSSVLFKEELEDLKDTYLQRFNLVFVLSR 180 Query: 181 EQQDVELFNGRIDRSKCDAFFASWIDLKDVDAAFLCGPEEMVQAVSDSLQAHGLPKTQIK 240 EQ D++LFNGRID K +A W+ +D+D AF+CGP M++ VS +L +G+ K +IK Sbjct: 181 EQLDIDLFNGRIDGEKVNALLKHWVKPQDIDIAFICGPHSMMEEVSQALLDNGVDKARIK 240 Query: 241 TELFAASAPT---RAHASRPVVGKKECEVTVIVDGYHNVFTMDKEKESVLDAGLKHGIDL 297 ELFA S P+ AHA + VG+++CEVTVI DG FT++K KE+VLDA L GI+L Sbjct: 241 RELFATSIPSARPAAHAHKQ-VGQQQCEVTVIQDGRTRSFTLEKNKETVLDAALAQGIEL 299 Query: 298 RYSCKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQD 355 YSCKGGVC+TCRCK +EG+VDMD N+ALEDYE+ARGF+LSCQS+ VSDKL++DFDQ+ Sbjct: 300 PYSCKGGVCSTCRCKRIEGEVDMDVNFALEDYEVARGFILSCQSYAVSDKLVIDFDQE 357 >ref|YP_160277.1| hypothetical protein ebA5725 [Aromatoleum aromaticum EbN1] emb|CAI09376.1| similar to aerobic phenylacetate degradation protein [Aromatoleum aromaticum EbN1] Length = 358 Score = 482 bits (1241), Expect = e-134, Method: Composition-based stats. Identities = 228/357 (63%), Positives = 280/357 (78%), Gaps = 2/357 (0%) Query: 1 MSKFYPLTISGVKQETRDTIVVSFAVPPELQDTFSYQQGQHLTLRSEIKGEDLRRSYSIC 60 MSKF+ + ++ V+ ETRD IVV+F VPP L D+F Y GQHLTLR+EI GED+RRSYSIC Sbjct: 1 MSKFHDMRVASVRPETRDAIVVTFDVPPSLADSFHYAPGQHLTLRTEIGGEDVRRSYSIC 60 Query: 61 SAVQDDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNVPLDAANRKHYLAFAA 120 S + LR+AIKR GG+FS WAND +KPG S++VMPP GHF V D + ++Y AFAA Sbjct: 61 SGADERALRIAIKRVHGGLFSNWANDFMKPGVSVEVMPPSGHFGVAPDPQHHRNYAAFAA 120 Query: 121 GSGITPMISIIKTTLLAEPHSRFTLVYANRASSSVIFKEDITDLKDAYLERLNVIWVMSR 180 GSGITP++S IKT L AE SRFTLVY NR+SSSV+F+E++ DLKD Y ERLN++ V+SR Sbjct: 121 GSGITPILSQIKTILAAEQGSRFTLVYGNRSSSSVMFREELADLKDQYGERLNLVHVLSR 180 Query: 181 EQQDVELFNGRIDRSKCDAFFASWIDLKDVDAAFLCGPEEMVQAVSDSLQAHGLPKTQIK 240 EQQD+ELFNGRIDR +CDA WID K +D AF+CGP +M++AVS SLQAHG+ K+ IK Sbjct: 181 EQQDIELFNGRIDRERCDALLTRWIDAKRIDVAFICGPMDMMEAVSASLQAHGVAKSAIK 240 Query: 241 TELFAASAPT--RAHASRPVVGKKECEVTVIVDGYHNVFTMDKEKESVLDAGLKHGIDLR 298 ELFA S P R S PV G ++CEVTVI DG FTM K ++SVLDAGL GI++ Sbjct: 241 MELFATSLPKGPRPVHSHPVKGTEDCEVTVIQDGRVRQFTMPKNRDSVLDAGLGQGIEMP 300 Query: 299 YSCKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQD 355 YSCKGGVC+TCRCKV++G+VD D N+ALEDYEIARGF LSCQS+PV+DKL+LDFDQ+ Sbjct: 301 YSCKGGVCSTCRCKVIDGEVDHDTNFALEDYEIARGFRLSCQSYPVTDKLVLDFDQE 357 >ref|YP_840878.1| phenylacetic acid degradation protein E,flavodoxin reductase [Ralstonia eutropha H16] emb|CAJ96148.1| phenylacetic acid degradation protein E,flavodoxin reductase [Ralstonia eutropha H16] Length = 358 Score = 478 bits (1229), Expect = e-133, Method: Composition-based stats. Identities = 226/357 (63%), Positives = 283/357 (79%), Gaps = 2/357 (0%) Query: 1 MSKFYPLTISGVKQETRDTIVVSFAVPPELQDTFSYQQGQHLTLRSEIKGEDLRRSYSIC 60 MSKF+ LT++ V +ETRD + V+FAVP EL DT+ Y QGQHLTLR+ I GED+RRSYSIC Sbjct: 1 MSKFHELTVASVTRETRDAVAVTFAVPDELADTYRYIQGQHLTLRTGIGGEDVRRSYSIC 60 Query: 61 SAVQDDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNVPLDAANRKHYLAFAA 120 SAVQD QLRVAIKR GG+FS WAN+ L+PG L+VMPP GHF+VPL A +HY+AFAA Sbjct: 61 SAVQDAQLRVAIKRVDGGLFSNWANEQLQPGMKLEVMPPSGHFHVPLSATQARHYVAFAA 120 Query: 121 GSGITPMISIIKTTLLAEPHSRFTLVYANRASSSVIFKEDITDLKDAYLERLNVIWVMSR 180 GSGITPM+SIIKTTL AEP SRFTL Y NRASSSV+FKE++ DLKD YL+R N+++V+SR Sbjct: 121 GSGITPMLSIIKTTLEAEPQSRFTLFYGNRASSSVLFKEELEDLKDTYLQRFNLVFVLSR 180 Query: 181 EQQDVELFNGRIDRSKCDAFFASWIDLKDVDAAFLCGPEEMVQAVSDSLQAHGLPKTQIK 240 EQ D++LFNGRID K +A W+ +D+D AF+CGP M++ VS +L +G+ K +IK Sbjct: 181 EQLDIDLFNGRIDGEKVNALLKHWVKPQDIDIAFICGPHSMMEEVSQALLDNGVDKARIK 240 Query: 241 TELFAAS--APTRAHASRPVVGKKECEVTVIVDGYHNVFTMDKEKESVLDAGLKHGIDLR 298 ELFA S A A + VG+++CEVTVI DG FT++K KE+VLDA L GI+L Sbjct: 241 RELFATSIPAARPAAHAHKQVGQQQCEVTVIQDGRTRSFTLEKNKETVLDAALAQGIELP 300 Query: 299 YSCKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQD 355 YSCKGGVC+TCRCK +EG+VDMD N+ALEDYE+ARGF+LSCQS+ VSD+L++DFDQ+ Sbjct: 301 YSCKGGVCSTCRCKRIEGEVDMDVNFALEDYEVARGFILSCQSYAVSDRLVIDFDQE 357 >ref|YP_297941.1| phenylacetate-CoA oxygenase/reductase subunit PaaK [Ralstonia eutropha JMP134] gb|AAZ63097.1| Phenylacetate-CoA oxygenase/reductase, PaaK subunit [Ralstonia eutropha JMP134] Length = 358 Score = 477 bits (1228), Expect = e-132, Method: Composition-based stats. Identities = 223/357 (62%), Positives = 284/357 (79%), Gaps = 2/357 (0%) Query: 1 MSKFYPLTISGVKQETRDTIVVSFAVPPELQDTFSYQQGQHLTLRSEIKGEDLRRSYSIC 60 MSKF+ LT++ V +ETRD + V+FAVP +L DT+ Y QGQHLTLR+ I GED+RRSYSIC Sbjct: 1 MSKFHELTVASVTRETRDAVAVTFAVPEDLADTYRYVQGQHLTLRAGIGGEDVRRSYSIC 60 Query: 61 SAVQDDQLRVAIKRTQGGVFSTWANDTLKPGQSLQVMPPMGHFNVPLDAANRKHYLAFAA 120 SAVQD +LRVAIKR GG+FS WAND L+PG +L+VMPP GHF+VPL + KHY+AFAA Sbjct: 61 SAVQDRRLRVAIKRVDGGMFSNWANDALQPGMTLEVMPPSGHFHVPLSDTHSKHYVAFAA 120 Query: 121 GSGITPMISIIKTTLLAEPHSRFTLVYANRASSSVIFKEDITDLKDAYLERLNVIWVMSR 180 GSGITPM+SIIKTTL+ EP SRFTL Y NRASSSV+FKE++ DLKD YLER N+++++SR Sbjct: 121 GSGITPMLSIIKTTLMTEPDSRFTLFYGNRASSSVLFKEELEDLKDTYLERFNLVFILSR 180 Query: 181 EQQDVELFNGRIDRSKCDAFFASWIDLKDVDAAFLCGPEEMVQAVSDSLQAHGLPKTQIK 240 EQ D++LFNGRID K A W+ +D+D AF+CGP M++ VS +LQ +G+ K +IK Sbjct: 181 EQLDIDLFNGRIDGEKVRALLRHWVRPQDIDVAFICGPHSMMEEVSQALQENGVDKARIK 240 Query: 241 TELFAAS--APTRAHASRPVVGKKECEVTVIVDGYHNVFTMDKEKESVLDAGLKHGIDLR 298 ELFA S A A + +G+++CEVTVI DG FT++K KE++LDA L GI+L Sbjct: 241 RELFATSIPAARPAAHAHKQLGQQQCEVTVIQDGRTRQFTLEKNKETILDAALAQGIELP 300 Query: 299 YSCKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQD 355 YSCKGGVC+TCRCK +EG+VDMD N+ALEDYE+ARGF+LSCQS+ V+DKL++DFDQ+ Sbjct: 301 YSCKGGVCSTCRCKRIEGEVDMDVNFALEDYEVARGFILSCQSYAVTDKLVIDFDQE 357