BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2383.p (546 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775378.1| Kef-type potassium efflux transporter NAD-bi... 836 0.0 ref|YP_544769.1| potassium efflux system protein [Methylobacillu... 660 0.0 ref|YP_568873.1| potassium efflux system protein [Rhodopseudomon... 607 e-171 dbj|GAA20003.1| probable potassium efflux transporter [Pseudomon... 606 e-171 ref|ZP_01368538.1| hypothetical protein PaerPA_01005699 [Pseudom... 606 e-171 >ref|YP_003775378.1| Kef-type potassium efflux transporter NAD-binding protein [Herbaspirillum seropedicae SmR1] gb|ADJ63470.1| Kef-type potassium efflux transporter (NAD-binding component) protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 836 bits (2160), Expect = 0.0, Method: Composition-based stats. Identities = 506/545 (92%), Positives = 520/545 (95%) Query: 1 MPHHTPLIATIVGGLVLAFIFGTLAHRLKMPPLIGYLVAGIAIGPFTPGFVGDADLAQEL 60 MPHHTPLIATIV GLVLAFIFGTLAHRLKMPPL+GYLVAGIAIGPFTPGFVGDADLAQEL Sbjct: 1 MPHHTPLIATIVAGLVLAFIFGTLAHRLKMPPLVGYLVAGIAIGPFTPGFVGDADLAQEL 60 Query: 61 AEIGVILLMFGVGLHFSLKDLLSVKNIAIPGAVAQIAIATLLGMGLGWLLGWPFGEGFLF 120 AEIGVILLMFGVGLHFSLKDLLSVKNIAIPGAVAQI IATLLGM LGWLLGWP GEGFLF Sbjct: 61 AEIGVILLMFGVGLHFSLKDLLSVKNIAIPGAVAQITIATLLGMALGWLLGWPVGEGFLF 120 Query: 121 GLALSVASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGE 180 GLALSVASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGE Sbjct: 121 GLALSVASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGE 180 Query: 181 ALSGNAVLLTLGLTLGKVVAFVAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGF 240 ALSGNAVLLTLG+TLGKVVAF AVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGF Sbjct: 181 ALSGNAVLLTLGITLGKVVAFAAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGF 240 Query: 241 ALGSAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIL 300 ALGSAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSI+ Sbjct: 241 ALGSAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIV 300 Query: 301 VREPLLVLATVLIIVVGKSLAAWLIVRAFGHPNSTALTISASLAQIGEFSFILATLGLSL 360 REPLLVLATVLIIVVGKSL A LIVRAFG+PN TALTISASLAQIGEFSFILATLGLSL Sbjct: 301 AREPLLVLATVLIIVVGKSLGALLIVRAFGYPNGTALTISASLAQIGEFSFILATLGLSL 360 Query: 361 DLLSPMARDLILGGAILSILLNPLLFSLLDRYEARHAEPADVQDVPPTVDLQDHVVLVGY 420 DLLSPMARDLILGGAILSILLNPLLFSLLDRYEAR + D P V+LQDHVVLVG+ Sbjct: 361 DLLSPMARDLILGGAILSILLNPLLFSLLDRYEARQPKAPQGGDEPQKVELQDHVVLVGF 420 Query: 421 GRVGRGIGEKLRAQGKPFVVIEAQREHLDALRQDGVPALYGNAAQSELLKAAAVERARWL 480 GRVGRGIGE+LRAQGKPFVVIEAQ E+L+ALR GVPALYGNAAQSELL+AAA++RARWL Sbjct: 421 GRVGRGIGEQLRAQGKPFVVIEAQLENLEALRAAGVPALYGNAAQSELLQAAAIDRARWL 480 Query: 481 LVAIPEVFEAGQVIENALELNAGLKVVARAHSDAEIEHLEKHGAQRVIMGEREIARGMLE 540 LVAIPEVFEAGQVIENALELN GL+VVARAHSDAEIEHLEKHGAQRVIMGEREIARGMLE Sbjct: 481 LVAIPEVFEAGQVIENALELNPGLQVVARAHSDAEIEHLEKHGAQRVIMGEREIARGMLE 540 Query: 541 SVGQA 545 VGQ Sbjct: 541 LVGQG 545 >ref|YP_544769.1| potassium efflux system protein [Methylobacillus flagellatus KT] gb|ABE48928.1| Kef-type potassium/proton antiporter, CPA2 family [Methylobacillus flagellatus KT] Length = 555 Score = 660 bits (1703), Expect = 0.0, Method: Composition-based stats. Identities = 385/543 (70%), Positives = 453/543 (83%), Gaps = 8/543 (1%) Query: 4 HTPLIATIVGGLVLAFIFGTLAHRLKMPPLIGYLVAGIAIGPFTPGFVGDADLAQELAEI 63 HTPLIATIVGGLVLAFIFG LA+RL+MPPL+GYL+AG+ +GP TPGFV DADLA EL+EI Sbjct: 3 HTPLIATIVGGLVLAFIFGMLANRLRMPPLVGYLIAGVVVGPHTPGFVADADLAHELSEI 62 Query: 64 GVILLMFGVGLHFSLKDLLSVKNIAIPGAVAQIAIATLLGMGLGWLLGWPFGEGFLFGLA 123 GVILLMFGVGLHFSL+DLLSVK+IAIPGA+ QIAIATLLG+ + +GWP GEG +FGLA Sbjct: 63 GVILLMFGVGLHFSLRDLLSVKSIAIPGAIVQIAIATLLGLSMALAIGWPLGEGLIFGLA 122 Query: 124 LSVASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALS 183 LS ASTVVLL ALQERR++ET+RG+IA+GWLIVEDIAMVL LVL+P LSG+LGG G L Sbjct: 123 LSTASTVVLLTALQERRILETKRGQIAIGWLIVEDIAMVLTLVLVPVLSGMLGGDGNTLE 182 Query: 184 GNAVLLTLGLTLGKVVAFVAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALG 243 G+A+ T+ LT+GK++AFVAVML+VGRRVIPW+L R A TGSRELFRLAVLAIALGFALG Sbjct: 183 GSALFNTMALTMGKIIAFVAVMLLVGRRVIPWLLARTAATGSRELFRLAVLAIALGFALG 242 Query: 244 SAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSILVRE 303 + Y+FGVSFALGAFFAGMILSESELSHRAAEESLPLRDAF+VLFFVSVGMLFDPSILVRE Sbjct: 243 ATYIFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFAVLFFVSVGMLFDPSILVRE 302 Query: 304 PLLVLATVLIIVVGKSLAAWLIVRAFGHPNSTALTISASLAQIGEFSFILATLGLSLDLL 363 P L+LAT+ IIV+GKSLAA+ IV AF HP TALTISASLAQIGEFSFILA+LG SL LL Sbjct: 303 PWLILATLFIIVIGKSLAAFGIVLAFRHPLGTALTISASLAQIGEFSFILASLGASLGLL 362 Query: 364 SPMARDLILGGAILSILLNPLLFSLLDRYEA-----RHAEPADVQDVPPTVD---LQDHV 415 S RDLIL GAILSIL+NP+LF+ LDR + +P + T+ L H Sbjct: 363 SDTGRDLILSGAILSILINPMLFAWLDRLKPWLLNREEKKPLKGESGARTISYPHLHAHA 422 Query: 416 VLVGYGRVGRGIGEKLRAQGKPFVVIEAQREHLDALRQDGVPALYGNAAQSELLKAAAVE 475 VLVGYGRVG+ +G LR Q +PF+V+E Q E + +LR+ G+PAL GNAA +++AA ++ Sbjct: 423 VLVGYGRVGKLVGAALRQQDQPFIVLEDQPEVVQSLREQGIPALCGNAAAPGMMEAANIK 482 Query: 476 RARWLLVAIPEVFEAGQVIENALELNAGLKVVARAHSDAEIEHLEKHGAQRVIMGEREIA 535 ARWLLVAIP+ EAG +I++A ++N ++VVARAHSDA+IEHLEKHGA IMGEREIA Sbjct: 483 SARWLLVAIPDALEAGHIIQHARKINPQIEVVARAHSDAQIEHLEKHGASYTIMGEREIA 542 Query: 536 RGM 538 +GM Sbjct: 543 KGM 545 >ref|YP_568873.1| potassium efflux system protein [Rhodopseudomonas palustris BisB5] gb|ABE38972.1| Potassium efflux system protein [Rhodopseudomonas palustris BisB5] Length = 556 Score = 607 bits (1564), Expect = e-171, Method: Composition-based stats. Identities = 364/549 (66%), Positives = 433/549 (78%), Gaps = 8/549 (1%) Query: 3 HHTPLIATIVGGLVLAFIFGTLAHRLKMPPLIGYLVAGIAIGPFTPGFVGDADLAQELAE 62 H+TPLI+TIV GLVLAFIFG +A RL++ PL+GYL+AG+ IGPFTPG+V + ++A ELAE Sbjct: 2 HNTPLISTIVAGLVLAFIFGVVAQRLRISPLVGYLLAGVVIGPFTPGYVANQNIANELAE 61 Query: 63 IGVILLMFGVGLHFSLKDLLSVKNIAIPGAVAQIAIATLLGMGLGWLLGWPFGEGFLFGL 122 IG+ILLMFGVGLHFSLKDLLSVK IAIPGAV QIA AT LG+GLG +LGW FG G +FGL Sbjct: 62 IGIILLMFGVGLHFSLKDLLSVKAIAIPGAVVQIATATALGIGLGHVLGWSFGSGLVFGL 121 Query: 123 ALSVASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEAL 182 ALSVASTVVLL+ALQERRLV++ RGRIAVGWLIVED+AMVL LVL+PA++G+L G Sbjct: 122 ALSVASTVVLLRALQERRLVDSDRGRIAVGWLIVEDLAMVLTLVLLPAVAGLLKGDSTGA 181 Query: 183 SGNAVLLTLGLTLGKVVAFVAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFAL 242 S + V L + LTLGKV AF+A ML+VGRRVIPW+L +A TGSRELFRLAVL+I+LG A Sbjct: 182 SWDTVALPIALTLGKVAAFIAFMLLVGRRVIPWMLHYVAHTGSRELFRLAVLSISLGVAF 241 Query: 243 GSAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSILVR 302 G+A +F VSFALGAFFAGMILSESELS RAA E+LPLRDAF+VLFFVSVGML DP IL+R Sbjct: 242 GAAILFDVSFALGAFFAGMILSESELSQRAASETLPLRDAFAVLFFVSVGMLVDPIILIR 301 Query: 303 EPLLVLATVLIIVVGKSLAAWLIVRAFGHPNSTALTISASLAQIGEFSFILATLGLSLDL 362 EPL VLATVLIIV GKSLAA+LIVR FGHPN TALTISASLAQIGEFSFILA+LG+SL L Sbjct: 302 EPLPVLATVLIIVFGKSLAAFLIVRLFGHPNMTALTISASLAQIGEFSFILASLGVSLAL 361 Query: 363 LSPMARDLILGGAILSILLNPLLFSLLDRYEARHAEPADVQDVPPT--------VDLQDH 414 L RDL+L GAI+SILLNPL+F+LLDR+ AR + + + V H Sbjct: 362 LPERGRDLVLAGAIISILLNPLMFALLDRFSARSGAAPEPEALAKETAGAHAKPVHHGGH 421 Query: 415 VVLVGYGRVGRGIGEKLRAQGKPFVVIEAQREHLDALRQDGVPALYGNAAQSELLKAAAV 474 VVLVGYGRVG +G LR G VVIE E + L+++G+ A+ GNAA ++L+AA + Sbjct: 422 VVLVGYGRVGSIVGAALRRDGADVVVIEDDTERVAQLQREGITAVGGNAAAPDMLEAAEI 481 Query: 475 ERARWLLVAIPEVFEAGQVIENALELNAGLKVVARAHSDAEIEHLEKHGAQRVIMGEREI 534 AR LLVAIP+ FE GQ++ A LNA L ++ARAHS E+ HL+ +GA VIMGE EI Sbjct: 482 ASARGLLVAIPDAFEGGQIVAKARALNAKLPIIARAHSTEEVAHLKSNGADVVIMGEDEI 541 Query: 535 ARGMLESVG 543 AR M+ +G Sbjct: 542 ARAMIARIG 550 >dbj|GAA20003.1| probable potassium efflux transporter [Pseudomonas aeruginosa NCMG1179] Length = 567 Score = 606 bits (1563), Expect = e-171, Method: Composition-based stats. Identities = 358/552 (64%), Positives = 440/552 (79%), Gaps = 13/552 (2%) Query: 3 HHTPLIATIVGGLVLAFIFGTLAHRLKMPPLIGYLVAGIAIGPFTPGFVGDADLAQELAE 62 HHTPL+ T+ G VLAF+FG LA+RL++ PL+GYL+AG+ GPFTPG+V D L+QE+AE Sbjct: 2 HHTPLLTTLAAGFVLAFLFGALANRLRISPLVGYLLAGVLAGPFTPGYVADQALSQEIAE 61 Query: 63 IGVILLMFGVGLHFSLKDLLSVKNIAIPGAVAQIAIATLLGMGLGWLLGWPFGEGFLFGL 122 +GVILLMFGVGLHFSLKDLLSVK IAIPGA+ QI +ATLLG+GL LLGW FG G +FGL Sbjct: 62 LGVILLMFGVGLHFSLKDLLSVKAIAIPGALVQIGVATLLGIGLAKLLGWSFGGGLVFGL 121 Query: 123 ALSVASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEAL 182 +LSVASTVVLL+AL++R+L++T+RGRIA+GWLIVED+AMVL LVL+PAL+G LGGK + Sbjct: 122 SLSVASTVVLLRALEQRQLLDTKRGRIAIGWLIVEDLAMVLTLVLLPALAGTLGGKTDG- 180 Query: 183 SGNAVLL-TLGLTLGKVVAFVAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFA 241 G+A LL L +TLGKV AFVA+M+ GRRV+PW+LERIA +GSRELF LAVLAIALG A Sbjct: 181 -GDAGLLWPLLITLGKVAAFVALMIFGGRRVVPWVLERIAASGSRELFTLAVLAIALGIA 239 Query: 242 LGSAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSILV 301 GSA +FGVSFALGAFFAGMIL+ESELSH AAE SLPLRDAF+VLFFVSVGMLF+P IL+ Sbjct: 240 YGSAVLFGVSFALGAFFAGMILNESELSHEAAENSLPLRDAFAVLFFVSVGMLFNPMILL 299 Query: 302 REPLLVLATVLIIVVGKSLAAWLIVRAFGHPNSTALTISASLAQIGEFSFILATLGLSLD 361 +EPL VLAT L+IV GKS+AA+LIVRAFGHPNSTAL I+ASLAQIGEFSFIL LG+ L+ Sbjct: 300 QEPLTVLATFLVIVFGKSVAAYLIVRAFGHPNSTALVIAASLAQIGEFSFILIGLGIGLE 359 Query: 362 LLSPMARDLILGGAILSILLNPLLFSLLDRY----EARHAEPAD------VQDVPPTVDL 411 L+ +RDL+L GAILSIL+NPLLF +DR E R AE A QD+PP + Sbjct: 360 LVPEQSRDLVLAGAILSILVNPLLFIAIDRLQQRKEQRLAEAAGGVREMPAQDLPPPILE 419 Query: 412 QDHVVLVGYGRVGRGIGEKLRAQGKPFVVIEAQREHLDALRQDGVPALYGNAAQSELLKA 471 ++H VL+G+GRVG + +LR G PFVV+ +R+ D LR+ G + GNAA +L+ Sbjct: 420 REHAVLIGHGRVGELVSARLRTAGTPFVVVVDKRDKADELREQGYSVVLGNAASPGVLEL 479 Query: 472 AAVERARWLLVAIPEVFEAGQVIENALELNAGLKVVARAHSDAEIEHLEKHGAQRVIMGE 531 A + ARWLL+AIP FEAGQ+ +A +N L +VARAH DAE+++LE++GA VIMGE Sbjct: 480 ANIAEARWLLIAIPNGFEAGQIASHARAVNPPLDIVARAHFDAEVDYLEQNGANLVIMGE 539 Query: 532 REIARGMLESVG 543 REIA GM+ +G Sbjct: 540 REIACGMVHRLG 551 >ref|ZP_01368538.1| hypothetical protein PaerPA_01005699 [Pseudomonas aeruginosa PACS2] ref|YP_793995.1| putative potassium efflux transporter [Pseudomonas aeruginosa UCBPP-PA14] ref|YP_002443491.1| putative potassium efflux transporter [Pseudomonas aeruginosa LESB58] ref|ZP_04936390.1| hypothetical protein PA2G_03858 [Pseudomonas aeruginosa 2192] ref|ZP_06881858.1| putative potassium efflux transporter [Pseudomonas aeruginosa PAb1] gb|ABJ14904.1| putative potassium efflux protein [Pseudomonas aeruginosa UCBPP-PA14] gb|EAZ60509.1| hypothetical protein PA2G_03858 [Pseudomonas aeruginosa 2192] emb|CAW30668.1| probable potassium efflux transporter [Pseudomonas aeruginosa LESB58] gb|EGM21083.1| putative potassium efflux transporter [Pseudomonas aeruginosa 138244] gb|EGM23766.1| putative potassium efflux transporter [Pseudomonas aeruginosa 152504] gb|AEO78117.1| putative potassium efflux transporter [Pseudomonas aeruginosa M18] Length = 567 Score = 606 bits (1562), Expect = e-171, Method: Composition-based stats. Identities = 359/552 (65%), Positives = 441/552 (79%), Gaps = 13/552 (2%) Query: 3 HHTPLIATIVGGLVLAFIFGTLAHRLKMPPLIGYLVAGIAIGPFTPGFVGDADLAQELAE 62 HHTPL+ T+ G VLAF+FG LA+RL++ PL+GYL+AG+ GPFTPG+V D L+QE+AE Sbjct: 2 HHTPLLTTLAAGFVLAFLFGALANRLRISPLVGYLLAGVLAGPFTPGYVADQALSQEIAE 61 Query: 63 IGVILLMFGVGLHFSLKDLLSVKNIAIPGAVAQIAIATLLGMGLGWLLGWPFGEGFLFGL 122 +GVILLMFGVGLHFSLKDLLSVK IAIPGA+ QI +ATLLG+GL LLGW FG G +FGL Sbjct: 62 LGVILLMFGVGLHFSLKDLLSVKAIAIPGALVQIGVATLLGIGLAKLLGWSFGGGLVFGL 121 Query: 123 ALSVASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEAL 182 +LSVASTVVLL+AL++R+L++T+RGRIA+GWLIVED+AMVL LVL+PAL+G LGGK + Sbjct: 122 SLSVASTVVLLRALEQRQLLDTKRGRIAIGWLIVEDLAMVLTLVLLPALAGTLGGKTDG- 180 Query: 183 SGNAVLL-TLGLTLGKVVAFVAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFA 241 G+A LL L +TLGKV AFVA+M+ GRRV+PW+LERIA +GSRELF LAVLAIALG A Sbjct: 181 -GDAGLLWPLLITLGKVAAFVALMIFGGRRVVPWVLERIAASGSRELFTLAVLAIALGIA 239 Query: 242 LGSAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSILV 301 GSA +FGVSFALGAFFAGMIL+ESELSH AAE SLPLRDAF+VLFFVSVGMLF+P IL+ Sbjct: 240 YGSAVLFGVSFALGAFFAGMILNESELSHEAAENSLPLRDAFAVLFFVSVGMLFNPMILL 299 Query: 302 REPLLVLATVLIIVVGKSLAAWLIVRAFGHPNSTALTISASLAQIGEFSFILATLGLSLD 361 +EPL VLAT L+IV GKS+AA+LIVRAFGHPNSTAL I+ASLAQIGEFSFIL LG+ L+ Sbjct: 300 QEPLTVLATFLVIVFGKSVAAYLIVRAFGHPNSTALVIAASLAQIGEFSFILIGLGIGLE 359 Query: 362 LLSPMARDLILGGAILSILLNPLLFSLLDRY----EARHAEPAD------VQDVPPTVDL 411 L+ +RDL+L GAILSIL+NPLLF +DR E R AE A QD+PP + Sbjct: 360 LVPEQSRDLVLAGAILSILVNPLLFIAIDRLQQRKEQRLAEAAGGVREMPAQDLPPPILE 419 Query: 412 QDHVVLVGYGRVGRGIGEKLRAQGKPFVVIEAQREHLDALRQDGVPALYGNAAQSELLKA 471 ++H VL+G+GRVG + +LRA G PFVV+ +R+ D LR+ G + GNAA +L+ Sbjct: 420 REHAVLIGHGRVGGLVSARLRAAGTPFVVVVDKRDKADELREQGYSVVLGNAASPGVLEL 479 Query: 472 AAVERARWLLVAIPEVFEAGQVIENALELNAGLKVVARAHSDAEIEHLEKHGAQRVIMGE 531 A + ARWLL+AIP FEAGQ+ +A +N L +VARAH DAE+++LE++GA VIMGE Sbjct: 480 ANIAEARWLLIAIPNGFEAGQIASHARAVNPPLDIVARAHFDAEVDYLEQNGANLVIMGE 539 Query: 532 REIARGMLESVG 543 REIA GM+ +G Sbjct: 540 REIACGMVHRLG 551