BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= 0001.2243.p (437 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 15,459,386 sequences; 5,307,515,862 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|YP_003775256.1| major facilitator superfamily permease [Herb... 696 0.0 ref|YP_003280191.1| major facilitator superfamily protein [Comam... 624 e-176 ref|ZP_07047285.1| major facilitator superfamily MFS_1 [Comamona... 623 e-176 ref|YP_004490411.1| major facilitator superfamily protein [Delft... 619 e-175 ref|ZP_03541210.1| major facilitator superfamily MFS_1 [Comamona... 618 e-175 >ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] gb|ADJ63348.1| permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 696 bits (1795), Expect = 0.0, Method: Composition-based stats. Identities = 412/437 (94%), Positives = 427/437 (97%) Query: 1 MSYSPSAAATAAPPASSSASLEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 MSYSPSAAAT A AS++A LE TY KIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD Sbjct: 1 MSYSPSAAATPAAHASAAAPLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 Query: 61 LQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPI 120 LQFSEAVYGLGAGIFFIGYF+FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTP Sbjct: 61 LQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPT 120 Query: 121 SFYVLRFLLGIFEAGFFPGVVLYLTYWFPAKRRARVNGLFMTSFAIAGAVGGPIAGLIMS 180 SFY+LRFLLGIFEAGFFPGVVLYLTYWFPA+RRARVNGLFMTSFAIAGAVGGPIAGLIMS Sbjct: 121 SFYILRFLLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMS 180 Query: 181 SMEGVGHLANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSDAEKTAVTQVLAAENS 240 MEGVGHLANWQWLFI+EGIPSILAGFAVLAWLPE+PANAKWLS+AEK AVTQVLAAENS Sbjct: 181 GMEGVGHLANWQWLFILEGIPSILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENS 240 Query: 241 APGKHFSFKDACTNYRVWVCAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIP 300 APGKHFSFKDACTNYRVW+CAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIP Sbjct: 241 APGKHFSFKDACTNYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIP 300 Query: 301 FVAGTLAMVWNGFHSDKTGERRLHSAIATLIACIGLIATGMTLGNATLALCSLTLAAIGI 360 F+AGTLAM+WNGFHSDKTGERRLHSAIATLIACIGL+ATGMTLGNATLALCSLTLAAIGI Sbjct: 301 FIAGTLAMIWNGFHSDKTGERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGI 360 Query: 361 LAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA 420 LAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA Sbjct: 361 LAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA 420 Query: 421 ALEFLASFLVVVFVKKH 437 ALE +ASFLVVVF++KH Sbjct: 421 ALELVASFLVVVFIRKH 437 >ref|YP_003280191.1| major facilitator superfamily protein [Comamonas testosteroni CNB-2] gb|ACY34895.1| major facilitator superfamily MFS_1 [Comamonas testosteroni CNB-2] Length = 439 Score = 624 bits (1608), Expect = e-176, Method: Composition-based stats. Identities = 359/439 (81%), Positives = 388/439 (88%), Gaps = 2/439 (0%) Query: 1 MSYSPSAAATAAPPASSSA--SLEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQML 58 MS SA A A + A ++ATY KI WRLIPFLVFLFVLAWIDRVNVGFAKLQML Sbjct: 1 MSSPASATHPLAGHAGAHAHPDMDATYRKITWRLIPFLVFLFVLAWIDRVNVGFAKLQML 60 Query: 59 QDLQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTT 118 QDLQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWG ASMAMAYVTT Sbjct: 61 QDLQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGAASMAMAYVTT 120 Query: 119 PISFYVLRFLLGIFEAGFFPGVVLYLTYWFPAKRRARVNGLFMTSFAIAGAVGGPIAGLI 178 P FYVLRF+LG+ EAGFFPGVVLYLTYWFPA+ RAR+NGLFMTSFAIAGAVGGPIAG I Sbjct: 121 PTMFYVLRFILGVVEAGFFPGVVLYLTYWFPARHRARINGLFMTSFAIAGAVGGPIAGAI 180 Query: 179 MSSMEGVGHLANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSDAEKTAVTQVLAAE 238 M+ M+ VGHLANWQWLFI+EGIPS++AGF VLAWLPEKP NAKWLS AE+ AV+ +A E Sbjct: 181 MNGMQDVGHLANWQWLFILEGIPSVIAGFFVLAWLPEKPENAKWLSVAEQRAVSAAVAQE 240 Query: 239 NSAPGKHFSFKDACTNYRVWVCAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISA 298 + K SF DAC NYRVW+CAAVYFC+VSGNATIAFW+PSIIKEIG++ NLQIGLISA Sbjct: 241 SQQGHKQHSFADACRNYRVWLCAAVYFCIVSGNATIAFWSPSIIKEIGIQNNLQIGLISA 300 Query: 299 IPFVAGTLAMVWNGFHSDKTGERRLHSAIATLIACIGLIATGMTLGNATLALCSLTLAAI 358 IPF+AGTLAMVWNG HSDKTGERR+HSAI LIA GLI TGM L +A LALC+LTLA++ Sbjct: 301 IPFLAGTLAMVWNGMHSDKTGERRMHSAIPALIAATGLILTGMFLHDAVLALCALTLASV 360 Query: 359 GILAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYF 418 GILAAFPVFWSIP AFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYF Sbjct: 361 GILAAFPVFWSIPSAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYF 420 Query: 419 VAALEFLASFLVVVFVKKH 437 VAALEFLA+FLVV+FVKK Sbjct: 421 VAALEFLAAFLVVLFVKKQ 439 >ref|ZP_07047285.1| major facilitator superfamily MFS_1 [Comamonas testosteroni S44] gb|EFI59086.1| major facilitator superfamily MFS_1 [Comamonas testosteroni S44] Length = 439 Score = 623 bits (1606), Expect = e-176, Method: Composition-based stats. Identities = 358/439 (81%), Positives = 388/439 (88%), Gaps = 2/439 (0%) Query: 1 MSYSPSAAATAAPPASSSA--SLEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQML 58 MS SA A A + A ++ATY KI WRLIPFLVFLFVLAWIDRVNVGFAKLQML Sbjct: 1 MSSPASATHPLAGHAGAHAHPDMDATYRKITWRLIPFLVFLFVLAWIDRVNVGFAKLQML 60 Query: 59 QDLQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTT 118 QDLQFSEAVYGLGAGIFFIGYFLFEVPSNLLLE+IGARKTLARITILWG ASMAMAYVTT Sbjct: 61 QDLQFSEAVYGLGAGIFFIGYFLFEVPSNLLLERIGARKTLARITILWGAASMAMAYVTT 120 Query: 119 PISFYVLRFLLGIFEAGFFPGVVLYLTYWFPAKRRARVNGLFMTSFAIAGAVGGPIAGLI 178 P FYVLRF+LG+ EAGFFPGVVLYLTYWFPA+ RAR+NGLFMTSFAIAGAVGGPIAG I Sbjct: 121 PTMFYVLRFILGVVEAGFFPGVVLYLTYWFPARHRARINGLFMTSFAIAGAVGGPIAGAI 180 Query: 179 MSSMEGVGHLANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSDAEKTAVTQVLAAE 238 M+ M+ VGHLANWQWLFI+EGIPS++AGF VLAWLPEKP NAKWLS AE+ AV+ +A E Sbjct: 181 MNGMQDVGHLANWQWLFILEGIPSVIAGFFVLAWLPEKPENAKWLSAAEQRAVSAAVAQE 240 Query: 239 NSAPGKHFSFKDACTNYRVWVCAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISA 298 + K SF DAC NYRVW+CAAVYFC+VSGNATIAFW+PSIIKEIG++ NLQIGLISA Sbjct: 241 SQQGHKQHSFADACRNYRVWLCAAVYFCIVSGNATIAFWSPSIIKEIGIQNNLQIGLISA 300 Query: 299 IPFVAGTLAMVWNGFHSDKTGERRLHSAIATLIACIGLIATGMTLGNATLALCSLTLAAI 358 IPF+AGTLAMVWNG HSDKTGERR+HSAI LIA GLI TGM L +A LALC+LTLA++ Sbjct: 301 IPFLAGTLAMVWNGMHSDKTGERRMHSAIPALIAATGLILTGMFLHDAVLALCALTLASV 360 Query: 359 GILAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYF 418 GILAAFPVFWSIP AFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYF Sbjct: 361 GILAAFPVFWSIPSAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYF 420 Query: 419 VAALEFLASFLVVVFVKKH 437 VAALEFLA+FLVV+FVKK Sbjct: 421 VAALEFLAAFLVVLFVKKQ 439 >ref|YP_004490411.1| major facilitator superfamily protein [Delftia sp. Cs1-4] gb|AEF92056.1| major facilitator superfamily MFS_1 [Delftia sp. Cs1-4] Length = 439 Score = 619 bits (1596), Expect = e-175, Method: Composition-based stats. Identities = 354/437 (81%), Positives = 390/437 (89%), Gaps = 3/437 (0%) Query: 4 SPSAAATAAPPASSSAS---LEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 SP++AA + + AS ++ATY KIAWRLIPFLV LF+LAWIDRVNVGFAKLQML D Sbjct: 3 SPASAAHSPAHGAGQASPPDMDATYRKIAWRLIPFLVLLFILAWIDRVNVGFAKLQMLDD 62 Query: 61 LQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPI 120 LQFSEA+YGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWG ASMAMAYVTTP Sbjct: 63 LQFSEAIYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGAASMAMAYVTTPT 122 Query: 121 SFYVLRFLLGIFEAGFFPGVVLYLTYWFPAKRRARVNGLFMTSFAIAGAVGGPIAGLIMS 180 FYVLRF+LG+ EAGFFPGVVLYLTYWFPA+ RARVNGLFMTSFAIAGAVGGPIAG IM+ Sbjct: 123 MFYVLRFILGVVEAGFFPGVVLYLTYWFPARHRARVNGLFMTSFAIAGAVGGPIAGAIMN 182 Query: 181 SMEGVGHLANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSDAEKTAVTQVLAAENS 240 M+GVGHL+NWQWLFI+EGIPS++AGF VL +LPEKPANA+WLS AE+ AVT + AEN Sbjct: 183 GMQGVGHLSNWQWLFILEGIPSVIAGFFVLLYLPEKPANARWLSPAEQRAVTAAVEAENQ 242 Query: 241 APGKHFSFKDACTNYRVWVCAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIP 300 KH SF DAC NYRVW+CAAVYFC+VSGNATIAFW+PSIIKEIG++ LQIGLISAIP Sbjct: 243 GGHKHVSFLDACRNYRVWLCAAVYFCIVSGNATIAFWSPSIIKEIGIQNTLQIGLISAIP 302 Query: 301 FVAGTLAMVWNGFHSDKTGERRLHSAIATLIACIGLIATGMTLGNATLALCSLTLAAIGI 360 F+AGTLAMVWNG HSDKTGERR+H A++ LIA GLI TG+ L NA LALC+LTLA+IGI Sbjct: 303 FLAGTLAMVWNGMHSDKTGERRMHCALSALIAAAGLILTGLFLHNAVLALCALTLASIGI 362 Query: 361 LAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA 420 LAAFPVFWSIP AFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA Sbjct: 363 LAAFPVFWSIPAAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVA 422 Query: 421 ALEFLASFLVVVFVKKH 437 ALEF+A+FLVV+FVKK Sbjct: 423 ALEFMAAFLVVLFVKKQ 439 >ref|ZP_03541210.1| major facilitator superfamily MFS_1 [Comamonas testosteroni KF-1] gb|EED65496.1| major facilitator superfamily MFS_1 [Comamonas testosteroni KF-1] Length = 439 Score = 618 bits (1594), Expect = e-175, Method: Composition-based stats. Identities = 361/439 (82%), Positives = 391/439 (89%), Gaps = 2/439 (0%) Query: 1 MSYSPSAAATAAPPASSSA--SLEATYGKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQML 58 MS SA A A + A ++ATY KI WRLIPFLVFLFVLAWIDRVNVGFAKLQML Sbjct: 1 MSSPASATHPLAGHAGAHAHPDMDATYRKITWRLIPFLVFLFVLAWIDRVNVGFAKLQML 60 Query: 59 QDLQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTT 118 QDLQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWG+ASMAMAYVTT Sbjct: 61 QDLQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGVASMAMAYVTT 120 Query: 119 PISFYVLRFLLGIFEAGFFPGVVLYLTYWFPAKRRARVNGLFMTSFAIAGAVGGPIAGLI 178 P FYVLRF+LG+ EAGFFPGVVLYLTYWFPA+ RAR+NGLFMTSFAIAGAVGGPIAG I Sbjct: 121 PTMFYVLRFILGVVEAGFFPGVVLYLTYWFPARHRARINGLFMTSFAIAGAVGGPIAGAI 180 Query: 179 MSSMEGVGHLANWQWLFIIEGIPSILAGFAVLAWLPEKPANAKWLSDAEKTAVTQVLAAE 238 M+ M+ VGHLANWQWLFI+EGIPS++AGF VLAWLPEKP NAKWLS AE+ AV+ +A E Sbjct: 181 MNGMQDVGHLANWQWLFILEGIPSVIAGFFVLAWLPEKPENAKWLSAAEQRAVSAAVAEE 240 Query: 239 NSAPGKHFSFKDACTNYRVWVCAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISA 298 + K SF DAC NYRVW+CAAVYFC+VSGNATIAFW+PSIIKEIG++ NLQIGLISA Sbjct: 241 SQQGHKQHSFADACRNYRVWLCAAVYFCIVSGNATIAFWSPSIIKEIGIQNNLQIGLISA 300 Query: 299 IPFVAGTLAMVWNGFHSDKTGERRLHSAIATLIACIGLIATGMTLGNATLALCSLTLAAI 358 IPF+AGTLAMVWNG HSDKTGERR+HSAIA LIA GLI TGM L NA LALC+LTLA++ Sbjct: 301 IPFLAGTLAMVWNGMHSDKTGERRMHSAIAALIAAAGLILTGMFLHNAVLALCALTLASV 360 Query: 359 GILAAFPVFWSIPGAFLAGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYF 418 GILAAFPVFWSIP AFLAGTAAAGGIALINS+GNLAGFVAPYMIGALKTSTGSLSSGLYF Sbjct: 361 GILAAFPVFWSIPSAFLAGTAAAGGIALINSVGNLAGFVAPYMIGALKTSTGSLSSGLYF 420 Query: 419 VAALEFLASFLVVVFVKKH 437 VAALEFLA+FLVV+FVKKH Sbjct: 421 VAALEFLAAFLVVLFVKKH 439